Multiplex real-time PCR Method for the detection and quantification of DNA from the four transgenic soy traits DP-356043-5, DP-305423-1, MON 87701, and BPS-CV127-9 and lectin

According to the release of authorization, several new transgenic soy crops are expected to be present in the harvest of soy beans. In contrast to earlier times, methods for the detection of these transgenic crops are available already in advance. However, these methods are mainly still inefficient single real-time PCR methods. To increase the efficiency of product control, a multiplex quantitative real-time PCR system was developed and characterized for the four new transgenic soy traits DP-356043-5, DP-305423-1, MON 87701 and BPS-CV127-9. It showed amplification efficiency, correlation and sensitivity similar to the single PCR systems applied therein. To evaluate the robustness, an appropriate testing scheme was developed and applied for the first time on this multiplex real-time PCR system. It showed the robust amplification of all analytes also in case where conditions were varied. This system allows relative multiplex quantification and/or delta–delta Ct method quantification and proofed the applicability in routine.     

Palm oil deodorizer distillate as toughening agent in polylactide packaging films

Polylactide (PLA) is the most used biodegradable and biobased food packaging polymer for rigid containers and films. However, its low ductility is a hurdle for increasing its applications in flexible food packaging. A solution is the use of additives. Palm oil deodorizer distillate (PODC) is revealed to be an excellent additive promoting PLA ductility. PODC is a by‐product of vegetable oil refining, which is available in stable quality and in sufficient amounts. Amorphous PLA/PODC blends had an elongation at break of around 130% and that of semi‐crystalline blends was still around 55% compared to the initial 5% of neat PLA. At the same time the PLA rigidity and high glass transition temperatures were kept. PODC was also a very efficient processing aid, allowing for film blow extrusion. The blends were stable in properties during six months without exudation. They complied with legal norms of Food Contact Materials (EU 10/2011) and induced no sensorial alteration of packed food. Therefore PODC is a very interesting alternative to common plasticizers for the production of flexible PLA packaging films. © 2016 Society of Chemical Industry     

Genetic Distance and Age Affect the Cuticular Chemical Profiles of the Clonal Ant Cerapachys biroi

Although cuticular hydrocarbons (CHCs) have received much attention from biologists because of their important role in insect communication, few studies have addressed the chemical ecology of clonal species of eusocial insects. In this study we investigated whether and how differences in CHCs relate to the genetics and reproductive dynamics of the parthenogenetic ant Cerapachys biroi. We collected individuals of different ages and subcastes from several colonies belonging to four clonal lineages, and analyzed their cuticular chemical signature. CHCs varied according to colonies and clonal lineages in two independent data sets, and correlations were found between genetic and chemical distances between colonies. This supports the results of previous research showing that C. biroi workers discriminate between nestmates and non-nestmates, especially when they belong to different clonal lineages. In C. biroi, the production of individuals of a morphological subcaste specialized in reproduction is inversely proportional to colony-level fertility. As chemical signatures usually correlate with fertility and reproductive activity in social Hymenoptera, we asked whether CHCs could function as fertility-signaling primer pheromones determining larval subcaste fate in C. biroi. Interestingly, and contrary to findings for several other ant species, fertility and reproductive activity showed no correlation with chemical signatures, suggesting the absence of fertility related CHCs. This implies that other cues are responsible for subcaste differentiation in this species.     

Characterizing hydrocyclone performance for grit removal from wastewater treatment activated sludge plants

Typically, 15–45% of the mixed liquor (sludge) in biological wastewater treatment plants (WWTPs) consists of inorganic (fixed) suspended solids. A portion of these inorganic compounds is grit (sand) originating from the influent. Grit accumulation impacts WWTP design and operating costs as these unbiodegradable solids reduce the effective treatment capacity of the bioreactor and other unit operations that must be sized to carry this material.The goal of this study was to characterize the performance of a hydrocyclone to selectively separate grit from activated sludge. Laboratory experiments were conducted with a 13 mm diameter Krebs hydrocyclone treating sludge from eight WWTPs. Reduced efficiencies of 17 ± 7% on fixed suspended solids and 9 ± 6% on volatile suspended solids were obtained. Grade efficiency curves enabled the development of a modified definition for cut size useful for this application. The characterization of hydrocyclone performance for grit removal from activated sludge will enable modelling of the process for integration into wastewater treatment simulators used for process performance prediction and design.     

Tree-to-tree variation in stearic and oleic acid content in seed fat from Allanblackia floribunda from wild stands: Potential for tree breeding

We determined whether Allanblackia floribunda, a forest tree species that is valued for the fat extracted from its seeds, could be genetically improved for fruit/seed production by sampling 17–40 fruits from each of 70 trees that were distributed among four sites in wild stands. Fat was extracted from the seeds, and stearic and oleic acid content of the fat was estimated. Highly significant (P < 0.0001) between- and within-tree variation characterised fruits and seed characters. Between-site phenotypic variation was not significant. Stearic and oleic acid percentages in seed fat ranged from 44.16% to 66.12%, and from 24.95% to 48.42% per tree sample, respectively. Moderate repeatabilities were identified in fruit characters and mean seed mass. Moderate positive relationships were found between stearic and oleic acid percentages. Seed fat profiles were not found to vary with other fruit characters. Twenty “plus trees” were selected for breeding.     

Impact of oxygen transport properties on the kinetic modeling of polypropylene thermal oxidation. II. Effect of oxygen diffusivity

The kinetic model, established in a previous article (François‐Heude et al., J. Appl. Polym. Sci., in press) to predict the homogeneous oxidation in iPP films typically thinner than 100 µm, is now extended to simulate the oxidation profiles in thicker plates by coupling the oxygen diffusion and its consumption by the chemical reactions. In this perspective, oxygen transport properties (namely oxygen solubility, diffusivity, and permeability) are measured by permeametry on a reference iPP. These values are compared with an exhaustive compilation of literature data to evaluate their variability among the whole iPP family, which one has been reasonably ascribed to initial differences in polymer morphology, but also to evaluate their consistency, especially their temperature dependence between 20 and 140°C. Failing to simulate oxidation profiles, the kinetic model is then used as an inverse resolution method for estimating more satisfactory values of oxygen transport properties. It is thus evidenced that the crystallinity changes induced by thermal oxidation largely explains the dramatic decrease in oxygen penetration toward the sample core just after the induction period. A strategy aimed for introducing the relationship between the polymer crystalline morphology and oxygen transport properties into the kinetic model is given in the graphical abstract, although the effect of polymer polarity remains to be established prior to this implementation. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41562.     

Time‐space PGD for the rapid solution of 3D nonlinear parametrized problems in the many‐query context

This work deals with the question of the resolution of nonlinear problems for many different configurations in order to build a ‘virtual chart’ of solutions. The targeted problems are three‐dimensional structures driven by Chaboche‐type elastic‐viscoplastic constitutive laws. In this context, parametric analysis can lead to highly expensive computations when using a direct treatment. As an alternative, we present a technique based on the use of the time‐space proper generalized decomposition in the framework of the LATIN method. To speed up the calculations in the parametrized context, we use the fact that at each iteration of the LATIN method, an approximation over the entire time‐space domain is available. Then, a global reduced‐order basis is generated, reused and eventually enriched, by treating, one‐by‐one, all the various parameter sets. The novelty of the current paper is to develop a strategy that uses the reduced‐order basis for any new set of parameters as an initialization for the iterative procedure. The reduced‐order basis, which has been built for a set of parameters, is reused to build a first approximation of the solution for another set of parameters. An error indicator allows adding new functions to the basis only if necessary. The gain of this strategy for studying the influence of material or loading variability reaches the order of 25 in the industrial examples that are presented. Copyright © 2015 John Wiley & Sons, Ltd.     

Les glissements de terrain du quartier Bélouizdad, Constantine, Algérie

In Constantine, Belouizdad district landslides are extensive (Fig. 1). They occur along a hillside largely urbanized, and cover over 30 hectares. Dozens of houses were destroyed. The formations raised by these movements correspond to the alternating of thick Miocene clays and conglomerates. These contain several aquifers. The study of these landslides is conducted on the basis of recognition by deep boreholes equipped with piezometers and inclinometers. The inclinometric measurements have revealed failure surfaces sometimes deep (20 to 30 m) and north displacement, towards the Rhumel wadi. The use of a stability calculation software “Talren” shows on the one hand, the high sensitivity of unstable Miocene formations in the presence of water and on the other hand, a significant revival of the safety factor by reducing the pore pressure. The recommended solutions are therefore a lowering of underground water levels. Two techniques are recommended : deep drainage trench or galleries combined with radiating drainage holes.     

Effect of Thermomechanical Fatigue on Precipitation Microstructure in Two Precipitation-Hardened Cast Aluminum Alloys

Thermal loading induces modifications of the precipitation microstructure of Al–Si–Cu–Mg alloys. This study focuses on the effect of deformation on precipitation microstructure during thermomechanical loadings. Several specimens were thermomechanically cycled while others were exposed to the same thermal cycles without any mechanical loading. The nature and morphological characteristics of the precipitation microstructure of the thermomechanically cycled specimens are compared to those of the thermally aged ones, using transmission electron microscopy (TEM), in order to assess the effect of deformation on the precipitation microstructure and especially on the kinetics of precipitate growth. The absence of any significant effect of superimposed straining during thermal cycling is discussed. Implications for the prevision of yield strength degradation during service operation are briefly presented.