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排序方式: 共有345条查询结果,搜索用时 31 毫秒
101.
Alena Nprstkov Kateina Malínsk Lenka Zvesk Drbkov Elodie Billey Dagmar Nprstkov Eva Sýkorov Ccile Bousquet-Antonelli David Honys 《International journal of molecular sciences》2021,22(4)
ALBA DNA/RNA-binding proteins form an ancient family, which in eukaryotes diversified into two Rpp25-like and Rpp20-like subfamilies. In most studied model organisms, their function remains unclear, but they are usually associated with RNA metabolism, mRNA translatability and stress response. In plants, the enriched number of ALBA family members remains poorly understood. Here, we studied ALBA dynamics during reproductive development in Arabidopsis at the levels of gene expression and protein localization, both under standard conditions and following heat stress. In generative tissues, ALBA proteins showed the strongest signal in mature pollen where they localized predominantly in cytoplasmic foci, particularly in regions surrounding the vegetative nucleus and sperm cells. Finally, we demonstrated the involvement of two Rpp25-like subfamily members ALBA4 and ALBA6 in RNA metabolism in mature pollen supported by their co-localization with poly(A)-binding protein 3 (PABP3). Collectively, we demonstrated the engagement of ALBA proteins in male reproductive development and the heat stress response, highlighting the involvement of ALBA4 and ALBA6 in RNA metabolism, storage and/or translational control in pollen upon heat stress. Such dynamic re-localization of ALBA proteins in a controlled, developmentally and environmentally regulated manner, likely reflects not only their redundancy but also their possible functional diversification in plants. 相似文献
102.
Alena Pavelkova Pavel Kucharczyk Jiri Zednik Vladimir Sedlarik 《Journal of Polymer Research》2014,21(5):1-13
Combining different polymeric systems can be a useful tool to create new networks with different characteristics with respect to the starting materials. In this work, hydrogels composed of gellan gum (GG) and polyethylene glycol dimethacrylate (PEG-DMA) were realized to overcome the fragility problems of physical gels of GG, which limit their biological application as scaffold for tissue engineering. The two polymeric systems were combined using different synthetic approaches, with particular attention to the double network strategy (DN). The influence of several parameters on the mechanical properties, such as the time of diffusion and the molecular weight of PEG-DMA, were evaluated by rheological studies and compressive texture analyses. The hydrogels were also investigated for their ability to swell and release model molecules with different sterical hindrances, such as vitamin B12 and myoglobin. Finally, to estimate the biological safety of the hydrogels, their effect on mitochondrial function of human fibroblasts was investigated. 相似文献
103.
Jan Havlíček Kateřina Rubešová Vít Jakeš Romana Kučerková Alena Beitlerová Martin Nikl 《Journal of the American Ceramic Society》2022,105(6):4039-4045
A special group of luminescence materials are scintillators for thermal neutron detection. Due to the properties of neutrons (contrary to e.g. electrons or gamma photons), a different approach has to be utilized—the high content of atoms with the sufficient absorption cross-section toward neutrons and lower material density are needed. This work pursues a basic study of LiSrBO3 and LiSr4(BO3)3 doped with cerium or europium—materials promising as thermal neutron scintillators. These borates exhibit promising properties such as the high content of lithium and boron, suitable density, and proper environment for luminescent ions. Radioluminescence of these ceramic borates doped with cerium and europium was examined and promising samples were further characterized by photoluminescence spectroscopy and radioluminescence and photoluminescence kinetics measurement. The radioluminescence study of these borates is published for the first time. Cerium-doped borates, in particular, exhibit a very promising response to an X-ray excitation. 相似文献
104.
Alena Kubátová Yan Luo Jana Š?ávová S.M. Sadrameli Evguenii Kozliak Wayne Seames 《Fuel》2011,90(8):2598-2608
Triacylglycerols (TGs) are naturally occurring oils abundant in many crops. A series of batch uncatalyzed thermal decomposition experiments were performed using canola and soybean oils to explore pathways of TG cracking. A detailed gas chromatographic protocol based on mass spectrometric identification and flame ionization quantification was applied to the organic liquid product generated upon cracking. Reaction conditions were identified that resulted in a novel organic liquid product (OLP) composition compared to previously reported work. Under these conditions (temperatures within a 420-440 °C range) a new route for TG thermolysis was discovered in which cracking reactions of original TG-bound fatty acids were nearly complete and led to the formation of 15-25 wt.% C2-C10 linear saturated monocarboxylic acids and ca. 30% linear alkanes. Less than 2 wt.% C16-C18 fatty acids which were originally present in the feedstocks as glycerol triesters were found in the OLP. These reactions appear to be kinetically controlled due to abundant hydrogen formation. This route provides a significant enrichment of low-MW compounds in the OLP (65-70 wt.% being <C11) and thus may be considered as a new option for the production of replacement products for petroleum-based fuels and chemicals. 相似文献
105.
Kubátová A Herman J Steckler TS de Veij M Miller DJ Klunder EB Wai CM Hawthorne SB 《Environmental science & technology》2003,37(24):5757-5762
No disposal option exists for "mixed wastes" such as paint scrapings that are co-contaminated with polychlorinated biphenyls (PCBs) and radioactive metals. Either removal or destruction of the PCBs is required prior to disposal. Comparison of subcritical water dechlorination (350 degrees C, 1 h) of Aroclor 1254 in paint scrapings (180 ppm) and of standard Aroclor 1254 showed significantly enhanced dechlorination in the presence of paint. While no significant degradation was observed for standard Aroclor (no paint), the dechlorination of PCBs in paint was 99, 99, and 80% for the hepta-, hexa-, and pentachlorinated congeners, respectively, indicating that metals in the paint enhanced the dechlorination reactions. Adding metals to the standard Aroclor (no paint) reactions enhanced PCB dechlorination in subcritical water in descending order of activity: Pb approximately = Cu > Al > Zn > Fe. In the presence of both zerovalent and divalent lead and zerovalent copper in subcritical water (350 degrees C, 1 h), 99% of the Aroclor 1254 mixture (tetra- to heptachlorinated biphenyls) was dechlorinated. High dechlorination (ca. 95%) was also achieved with zerovalent aluminum. In contrast to other metals, lead retained its degradation ability at a lower temperature of 250 degrees C after 18 h. The high degradation efficiency achieved using metal additives in water at reasonable temperatures and pressures demonstrates the potential for subcritical water dechlorination of PCBs in paint scrapings and, potentially, in other solid and liquid wastes. 相似文献
106.
107.
A new method of impulse radio ultra-wideband (IR-UWB) pulse generation, with advantage of providing a “notch” like representation of pulse in the spectrum domain for particular control parameters values, is investigated in this paper. Low power pulse generator is composed of a glitch generator, a switched oscillator, a two-stage buffer and a pulse shaping filter. The proposed architecture, designed in UMC 0.18 µm CMOS technology, can operate in a single band from 3.3 GHz to 9.3 GHz or in a double, lower and higher UWB band (from 3 GHz to 9.15 GHz), suppressing frequencies in the WLAN band. Both spectrums fully comply with the corresponding FCC spectral mask, while the pulse generator regime and the spectrum range are determined by control signal values. Post-layout simulation results showed a pulse width of 0.5 ns, and a peak-to-peak amplitude of 211 mV for one band spectrum. The average power consumption is 0.89 mW corresponding to the energy consumption of 8.9 pJ/pulse for 100 MHz pulse repetition rate (PRF). The pulse duration is 1 ns and peak-to-peak amplitude is 202 mV in the case of the WLAN frequency band suppression. The total chip area is 0.31 mm2. The pulse generator has been evaluated for the best performance supporting the on-off keying (OOK) modulation. 相似文献
108.
Alena Y. Drobiazko Anastasia A. Kasimova Peter V. Evseev Mikhail M. Shneider Evgeniy I. Klimuk Alexander S. Shashkov Andrei S. Dmitrenok Alexander O. Chizhov Pavel V. Slukin Yuriy P. Skryabin Nikolay V. Volozhantsev Konstantin A. Miroshnikov Yuriy A. Knirel Anastasia V. Popova 《International journal of molecular sciences》2022,23(9)
In this study, several different depolymerases encoded in the prophage regions of Acinetobacter baumannii genomes have been bioinformatically predicted and recombinantly produced. The identified depolymerases possessed multi-domain structures and were identical or closely homologous to various proteins encoded in other A. baumannii genomes. This means that prophage-derived depolymerases are widespread, and different bacterial genomes can be the source of proteins with polysaccharide-degrading activities. For two depolymerases, the specificity to capsular polysaccharides (CPSs) of A. baumannii belonging to K1 and K92 capsular types (K types) was determined. The data obtained showed that the prophage-derived depolymerases were glycosidases that cleaved the A. baumannii CPSs by the hydrolytic mechanism to yield monomers and oligomers of the K units. The recombinant proteins with established enzymatic activity significantly reduced the mortality of Galleria mellonella larvae infected with A. baumannii of K1 and K92 capsular types. Therefore, these enzymes can be considered as suitable candidates for the development of new antibacterials against corresponding A. baumannii K types. 相似文献
109.
110.
Pavel Novk Alena Michalcov Jan erk Dalibor Vojtch Tom Fabin Simona Randkov Filip Pra Vítzslav Knotek Michal Novk 《Journal of Alloys and Compounds》2009,470(1-2):123-126
The isothermal section of the Dy–Co–Ti system at 500 °C has been investigated in the whole composition range by means of X-ray diffraction, thermal analysis, scanning electron microscopy and energy dispersive X-ray spectroscopy. The only ternary phase DyTixCo12−x is of ThMn12-type structure, space group I4/mmm, and shows a small homogeneity range of 1 ≤ x ≤ 1.6. The lattice parameters for DyTixCo12−x with 1 ≤ x ≤ 1.56 are a = 0.8336(4)–0.8402(1) nm and c = 0.4691(3)–0.4727(1) nm. Along a constant Dy concentration, the solid solubilities of Ti in the compounds Dy2Co17, DyCo3, DyCo2 and Dy3Co are about 2.0, 2.0, 3.0 and 4.0 at.%, respectively. The TiCo phase has a homogeneity range of 50–54 at.% Co at 500 °C and dissolves up to 2.0 at.% Dy. 相似文献