全文获取类型
收费全文 | 1202篇 |
免费 | 72篇 |
专业分类
电工技术 | 8篇 |
化学工业 | 523篇 |
金属工艺 | 9篇 |
机械仪表 | 23篇 |
建筑科学 | 31篇 |
能源动力 | 29篇 |
轻工业 | 278篇 |
水利工程 | 9篇 |
石油天然气 | 6篇 |
无线电 | 31篇 |
一般工业技术 | 146篇 |
冶金工业 | 32篇 |
原子能技术 | 3篇 |
自动化技术 | 146篇 |
出版年
2024年 | 4篇 |
2023年 | 22篇 |
2022年 | 126篇 |
2021年 | 135篇 |
2020年 | 55篇 |
2019年 | 42篇 |
2018年 | 40篇 |
2017年 | 50篇 |
2016年 | 38篇 |
2015年 | 29篇 |
2014年 | 53篇 |
2013年 | 65篇 |
2012年 | 78篇 |
2011年 | 96篇 |
2010年 | 62篇 |
2009年 | 78篇 |
2008年 | 61篇 |
2007年 | 51篇 |
2006年 | 39篇 |
2005年 | 25篇 |
2004年 | 25篇 |
2003年 | 19篇 |
2002年 | 22篇 |
2001年 | 6篇 |
2000年 | 8篇 |
1999年 | 9篇 |
1998年 | 6篇 |
1997年 | 9篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 1篇 |
排序方式: 共有1274条查询结果,搜索用时 15 毫秒
11.
Francesco Borrelli Carmen Del Vecchio Alessandra ParisioAuthor vitae 《Automatica》2009,45(12):2930-2936
Several items are produced and stored into n buffers in order to supply an external demand without interruptions. We consider the classical problem of determining control laws and smallest buffer levels guaranteeing that an unknown bounded demand is always satisfied. A simple model with n decoupled integrators and n additive bounded disturbances is employed. The coupling arises from bounds on the total production capacity and on the total demand. Invariant set theory for linear and switched linear systems is exploited to compute robust positive invariant sets and controlled robust invariant sets for two commonly adopted scheduling policies. This paper provides the explicit expression of the invariant sets for any arbitrary n. 相似文献
12.
Laura Micheli Lara Testai Andrea Angeli Donatello Carrino Alessandra Pacini Francesco Margiotta Lorenzo Flori Claudiu T. Supuran Vincenzo Calderone Carla Ghelardini Lorenzo Di Cesare Mannelli 《International journal of molecular sciences》2022,23(11)
Neuropathy development is a major dose-limiting side effect of anticancer treatments that significantly reduces patient’s quality of life. The inadequate pharmacological approaches for neuropathic pain management warrant the identification of novel therapeutic targets. Mitochondrial dysfunctions that lead to reactive oxygen species (ROS) increase, cytosolic Ca2+ imbalance, and lactate acidosis are implicated in neuropathic pain pathogenesis. It has been observed that in these deregulations, a pivotal role is played by the mitochondrial carbonic anhydrases (CA) VA and VB isoforms. Hence, preclinical studies should be conducted to assess the efficacy of two novel selenides bearing benzenesulfonamide moieties, named 5b and 5d, and able to inhibit CA VA and VB against paclitaxel-induced neurotoxicity in mice. Acute treatment with 5b and 5d (30–100 mg/kg, per os – p.o.) determined a dose-dependent and long-lasting anti-hyperalgesic effect in the Cold plate test. Further, repeated daily treatment for 15 days with 100 mg/kg of both compounds (starting the first day of paclitaxel injection) significantly prevented neuropathic pain development without the onset of tolerance to the anti-hyperalgesic effect. In both experiments, acetazolamide (AAZ, 100 mg/kg, p.o.) used as the reference drug was partially active. Moreover, ex vivo analysis demonstrated the efficacy of 5b and 5d repeated treatments in reducing the maladaptive plasticity that occurs to glia cells in the lumbar portion of the spinal cord and in improving mitochondrial functions in the brain and spinal cord that were strongly impaired by paclitaxel-repeated treatment. In this regard, 5b and 5d ameliorated the metabolic activity, as observed by the increase in citrate synthase activity, and preserved an optimal mitochondrial membrane potential (ΔΨ) value, which appeared depolarized in brains from paclitaxel-treated animals. In conclusion, 5b and 5d have therapeutic and protective effects against paclitaxel-induced neuropathy without tolerance development. Moreover, 5b and 5d reduced glial cell activation and mitochondrial dysfunction in the central nervous system, being a promising candidate for the management of neuropathic pain and neurotoxicity evoked by chemotherapeutic drugs. 相似文献
13.
Elisa Di Fabio Antonia Iazzetti Alessio Incocciati Valentina Caseli Giancarlo Fabrizi Alberto Boffi Alessandra Bonamore Alberto Macone 《International journal of molecular sciences》2022,23(12)
Amine oxidases are enzymes belonging to the class of oxidoreductases that are widespread, from bacteria to humans. The amine oxidase from Lathyrus cicera has recently appeared in the landscape of biocatalysis, showing good potential in the green synthesis of aldehydes. This enzyme catalyzes the oxidative deamination of a wide range of primary amines into the corresponding aldehydes but its use as a biocatalyst is challenging due to the possible inactivation that might occur at high product concentrations. Here, we show that the enzyme’s performance can be greatly improved by immobilization on solid supports. The best results are achieved using amino-functionalized magnetic microparticles: the immobilized enzyme retains its activity, greatly improves its thermostability (4 h at 75 °C), and can be recycled up to 8 times with a set of aromatic ethylamines. After the last reaction cycle, the overall conversion is about 90% for all tested substrates, with an aldehyde production ranging between 100 and 270 mg depending on the substrate used. As a proof concept, one of the aldehydes thus produced was successfully used for the biomimetic synthesis of a non-natural benzylisoquinoline alkaloid. 相似文献
14.
Anna Michelotti Marco de Scordilli Elisa Bertoli Elisa De Carlo Alessandro Del Conte Alessandra Bearz 《International journal of molecular sciences》2022,23(12)
Standard treatment for advanced non-small cell lung cancer (NSCLC) historically consisted of systemic cytotoxic chemotherapy until the early 2000s, when precision medicine led to a revolutionary change in the therapeutic scenario. The identification of oncogenic driver mutations in EGFR, ALK and ROS1 rearrangements identified a subset of patients who largely benefit from targeted agents. However, since the proportion of patients with druggable alterations represents a minority, the discovery of new potential driver mutations is still an urgent clinical need. We provide a comprehensive review of the emerging molecular targets in NSCLC and their applications in the advanced setting. 相似文献
15.
Annalisa Bernareggi Alessandra Bosutti Gabriele Massaria Rashid Giniatullin Tarja Malm Marina Sciancalepore Paola Lorenzon 《International journal of molecular sciences》2022,23(12)
Piezo1 channels are highly mechanically-activated cation channels that can sense and transduce the mechanical stimuli into physiological signals in different tissues including skeletal muscle. In this focused review, we summarize the emerging evidence of Piezo1 channel-mediated effects in the physiology of skeletal muscle, with a particular focus on the role of Piezo1 in controlling myogenic precursor activity and skeletal muscle regeneration and vascularization. The disclosed effects reported by pharmacological activation of Piezo1 channels with the selective agonist Yoda1 indicate a potential impact of Piezo1 channel activity in skeletal muscle regeneration, which is disrupted in various muscular pathological states. All findings reported so far agree with the idea that Piezo1 channels represent a novel, powerful molecular target to develop new therapeutic strategies for preventing or ameliorating skeletal muscle disorders characterized by an impairment of tissue regenerative potential. 相似文献
16.
Silvia Carra Germano Gaudenzi Alessandra Dicitore Maria Celeste Cantone Alice Plebani Davide Saronni Silvia Zappavigna Michele Caraglia Alessia Candeo Andrea Bassi Luca Persani Giovanni Vitale 《International journal of molecular sciences》2022,23(15)
Lung carcinoids are neuroendocrine tumors that comprise well-differentiated typical (TCs) and atypical carcinoids (ACs). Preclinical models are indispensable for cancer drug screening since current therapies for advanced carcinoids are not curative. We aimed to develop a novel in vivo model of lung carcinoids based on the xenograft of lung TC (NCI-H835, UMC-11, and NCI-H727) and AC (NCI-H720) cell lines and patient-derived cell cultures in Tg(fli1a:EGFP)y1 zebrafish embryos. We exploited this platform to test the anti-tumor activity of sulfatinib. The tumorigenic potential of TC and AC implanted cells was evaluated by the quantification of tumor-induced angiogenesis and tumor cell migration as early as 24 h post-injection (hpi). The characterization of tumor-induced angiogenesis was performed in vivo and in real time, coupling the tumor xenograft with selective plane illumination microscopy on implanted zebrafish embryos. TC-implanted cells displayed a higher pro-angiogenic potential compared to AC cells, which inversely showed a relevant migratory behavior within 48 hpi. Sulfatinib inhibited tumor-induced angiogenesis, without affecting tumor cell spread in both TC and AC implanted embryos. In conclusion, zebrafish embryos implanted with TC and AC cells faithfully recapitulate the tumor behavior of human lung carcinoids and appear to be a promising platform for drug screening. 相似文献
17.
Agnese Filippello Stefania Di Mauro Alessandra Scamporrino Sebastiano Alfio Torrisi Gian Marco Leggio Antonino Di Pino Roberto Scicali Maurizio Di Marco Roberta Malaguarnera Francesco Purrello Salvatore Piro 《International journal of molecular sciences》2022,23(14)
Intestinal cell dysfunctions involved in obesity and associated diabetes could be correlated with impaired intestinal cell development. To date, the molecular mechanisms underlying these dysfunctions have been poorly investigated because of the lack of a good model for studying obesity. The main aim of this study was to investigate the effects of lipotoxicity on intestinal cell differentiation in small intestinal organoid platforms, which are used to analyze the regulation of cell differentiation. Mouse intestinal organoids were grown in the presence/absence of high palmitate concentrations (0.5 mM) for 48 h to simulate lipotoxicity. Palmitate treatment altered the expression of markers involved in the differentiation of enterocytes and goblet cells in the early (Hes1) and late (Muc2) phases of their development, respectively, and it modified enterocytes and goblet cell numbers. Furthermore, the expression of enteroendocrine cell progenitors (Ngn3) and I cells (CCK) markers was also impaired, as well as CCK-positive cell numbers and CCK secretion. Our data indicate, for the first time, that lipotoxicity simultaneously influences the differentiation of specific intestinal cell types in the gut: enterocytes, goblet cells and CCK cells. Through this study, we identified novel targets associated with molecular mechanisms affected by lipotoxicity that could be important for obesity and diabetes therapy. 相似文献
18.
19.
20.