SPR-Based Detection of ASF Virus in Cells

African swine fever (ASF) is one of the most dangerous hemorrhagic infectious diseases that affect domestic and wild pigs. Currently, neither a vaccine nor effective treatments are available for this disease. As regards the degree of virulence, ASFV strains can be divided into high, moderate, or low virulence. The main detection methods are based on the use of the polymerase chain reaction (PCR). In order to prevent an uncontrolled spread of ASF, new on-site techniques that can enable the identification of an early-stage disease are needed. We have developed a specific immunological SPR-based assay for ASFV antigen detection directly in liquid samples. The developed assay allows us to detect the presence of ASFV at the dose of 10³ HAD₅₀/_mL.     

Cannabidiol Antiproliferative Effect in Triple-Negative Breast Cancer MDA-MB-231 Cells Is Modulated by Its Physical State and by IGF-1

Cannabidiol (CBD) is a non-psychoactive phytocannabinoid that has been discussed for its safety and efficacy in cancer treatments. For this reason, we have inquired into its use on triple-negative human breast cancer. Analyzing the biological effects of CBD on MDA-MB-231, we have demonstrated that both CBD dosage and serum concentrations in the culture medium influence its outcomes; furthermore, light scattering studies demonstrated that serum impacts the CBD aggregation state by acting as a surfactant agent. Pharmacological studies on CBD in combination with chemotherapeutic agents reveal that CBD possesses a protective action against the cytotoxic effect exerted by cisplatin on MDA-MB-231 grown in standard conditions. Furthermore, in a low serum condition (0.5%), starting from a threshold concentration (5 µM), CBD forms aggregates, exerts cytostatic antiproliferative outcomes, and promotes cell cycle arrest activating autophagy. At doses above the threshold, CBD exerts a highly cytotoxic effect inducing bubbling cell death. Finally, IGF-1 and EGF antagonize the antiproliferative effect of CBD protecting cells from harmful consequences of CBD aggregates. In conclusion, CBD effect is strongly associated with the physical state and concentration that reaches the treated cells, parameters not taken into account in most of the research papers.     

Therapeutic Potential of Allicin and Aged Garlic Extract in Alzheimer’s Disease

Garlic, Allium sativum, has long been utilized for a number of medicinal purposes around the world, and its medical benefits have been well documented. The health benefits of garlic likely arise from a wide variety of components, possibly working synergistically. Garlic and garlic extracts, especially aged garlic extracts (AGEs), are rich in bioactive compounds, with potent anti-inflammatory, antioxidant and neuroprotective activities. In light of these effects, garlic and its components have been examined in experimental models of Alzheimer’s disease (AD), the most common form of dementia without therapy, and a growing health concern in aging societies. With the aim of offering an updated overview, this paper reviews the chemical composition, metabolism and bioavailability of garlic bioactive compounds. In addition, it provides an overview of signaling mechanisms triggered by garlic derivatives, with a focus on allicin and AGE, to improve learning and memory.     

The Treatment of Advanced Melanoma: Therapeutic Update

Cutaneous melanoma is the main cause of death for skin cancer. The majority of patients with a diagnosis of melanoma have localized disease, which can be successfully treated with surgical treatment. However, the surgical approach is not curative for advanced melanoma (AM). Indeed, the management of AM is still challenging, since melanoma is the solid tumor with the highest number of mutations and cancer cells have the capacity to evade the immune system. In the past, the treatment of AM relied on chemotherapeutic agents, without showing efficacy data. Recent knowledge on melanoma pathogenesis as well as the introduction of immunotherapies, targeted therapies vaccines, small molecules, and combination therapies has revolutionized AM management, showing promising results in terms of effectiveness and safety. The aim of this review is to assess and to discuss the role of emerging therapies for AM management in order to obtain a complete overview of the currently available treatment options and future perspectives.     

Circulating U13 Small Nucleolar RNA as a Potential Biomarker in Huntington’s Disease: A Pilot Study

Plasma small RNAs have been recently explored as biomarkers in Huntington’s disease (HD). We performed an exploratory study on nine HD patients, eight healthy subjects (HS), and five psychiatric patients (PP; to control for iatrogenic confounder effects) through an Affymetrix-Gene-Chip-miRNA-Array. We validated the results in an independent population of 23 HD, 15 pre-HD, 24 PP, 28 Alzheimer’s disease (AD) patients (to control the disease-specificity) and 22 HS through real-time PCR. The microarray results showed higher levels of U13 small nucleolar RNA (SNORD13) in HD patients than controls (fold change 1.54, p = 0.003 HD vs. HS, and 1.44, p = 0.0026 HD vs. PP). In the validation population, a significant increase emerged with respect to both pre-HD and the control groups (p < 0.0001). SNORD13 correlated with the status of the mutant huntingtin carrier (r = 0.73; p < 0.001) and the disease duration (r = 0.59; p = 0.003). The receiver operating characteristic (ROC) curve analysis showed the high accuracy of SNORD13 in discriminating HD patients from other groups (AUC = 0.963). An interactome and pathway analysis on SNORD13 revealed enrichments for factors relevant to HD pathogenesis. We report the unprecedented finding of a potential disease-specific role of SNORD13 in HD. It seems to peripherally report a ‘tipping point’ in the pathogenic cascade at the neuronal level.     

Core − shell microgels with controlled structural properties

Microgels have gained great attention in the biomedical field for their wide application in diagnostic and drug delivery systems. The bulk properties as well as the surface features of these particular microparticles define their final performance. In particular, multifunctional microgels with complex architectures have been widely used in multiplex assays for their favourable capability to accommodate encoding systems and anchoring groups for probes to capture circulating targets by simply changing synthesis parameters. In this work a limited set of fluorescent encoded poly(ethyleneglycol) based microgels, of size ranging between 0.5 and 1.3 µm, with a core ? shell architecture were obtained by combining precipitation and seeded polymerizations. Here we demonstrate the possibility of tailoring and controlling the bulk and surface properties according to the synthesis by fluorescence imaging and pH titrations. Concerning the structural characterization, we adopted a method to calculate polymer fraction volumes from AFM images and combined these with equilibrium swelling theory (Peppas–Merrill equation) to determine the mesh size of the microgels. Surface composition was probed by X‐ray photoelectron spectroscopy directly on freeze‐dried microgels. In such a way we were able to describe the organizations of the different adlayers also in response to pH, highlighting the possibility of some overlap of the adlayers representing physical barriers at the boundaries of each shell. © 2016 Society of Chemical Industry     

Polymorphisms of Dopamine Receptor Genes and Parkinson’s Disease: Clinical Relevance and Future Perspectives

Parkinson’s disease (PD) is a neurodegenerative disease caused by loss of dopaminergic neurons in the midbrain. PD is clinically characterized by a variety of motor and nonmotor symptoms, and treatment relies on dopaminergic replacement. Beyond a common pathological hallmark, PD patients may present differences in both clinical progression and response to drug therapy that are partly affected by genetic factors. Despite extensive knowledge on genetic variability of dopaminergic receptors (DR), few studies have addressed their relevance as possible influencers of clinical heterogeneity in PD patients. In this review, we summarized available evidence regarding the role of genetic polymorphisms in DR as possible determinants of PD development, progression and treatment response. Moreover, we examined the role of DR in the modulation of peripheral immunity, in light of the emerging role of the peripheral immune system in PD pathophysiology. A better understanding of all these aspects represents an important step towards the development of precise and personalized disease-modifying therapies for PD.     

Experimental Analyses of the Evaporation Dynamics in Bare Soils under Natural Conditions

In spite of recent progresses in evaporation estimate through advanced models and laboratory experiments, the drying process of bare soils through its successive stages remains difficult to predict. A study which addresses evaporation modeling in natural bare soils is presented. It relies upon hydro-meteorological measurements performed in a plot with a bare silt loam soil maintained in natural conditions. The following steps are involved: estimate of daily actual evaporation, E_a, through the hydrological balance, scaling with pan evaporation measurements, E_pan, and analysis of the relation of E_a/E_pan with the soil moisture vertical profile. The results enable us (1) to check the occurrence of the first stage of the evaporation process, characterized by not limited-soil water supply and high evaporative rates, and (2) to identify the transition from the first to the second stage, with decreasing soil water-limited E_a values. The last point requires the introduction of a soil water content threshold at 5 cm depth, that is associated with the soil field capacity. The adopted procedure provides insights on the soil water dynamics at depths differently involved through the successive stages of the evaporative process. Finally, indications on the use of pan evaporation measurements in evaporative rate estimate at least during the first stage of the process are also given.     

ICT Governance: A Reference Framework

This article deals with information and communication technology (ICT) governance, a topic that has been discussed in the literature over the last 20 years. In order to offer a conceptualized view of the ICT governance models adopted by companies, a theoretical framework has been developed. A review of the available literature dealing with ICT governance, as well as a critical analysis of 27 case studies, was carried out, with the aim of highlighting the variables of the proposed framework.     

A real time algorithm for people tracking using contextual reasoning

In this paper we present a real-time tracking algorithm that is able to deal with complex occlusions involving a plurality of moving objects simultaneously. The rationale is grounded on a suitable representation and exploitation of the recent history of each single moving object being tracked. The object history is encoded using a state, and the transitions among the states are described through a Finite State Automata (FSA). In presence of complex situations the tracking is properly solved by making the FSA’s of the involved objects interact with each other. This is the way for basing the tracking decisions not only on the information present in the current frame, but also on conditions that have been observed more stably over a longer time span. The object history can be used to reliably discern the occurrence of the most common problems affecting object detection, making this method particularly robust in complex scenarios. An experimental evaluation of the proposed approach has been made on two publicly available datasets, the ISSIA Soccer Dataset and the PETS 2010 database.