全文获取类型
收费全文 | 2042篇 |
免费 | 152篇 |
国内免费 | 2篇 |
专业分类
电工技术 | 25篇 |
化学工业 | 496篇 |
金属工艺 | 52篇 |
机械仪表 | 90篇 |
建筑科学 | 31篇 |
矿业工程 | 1篇 |
能源动力 | 92篇 |
轻工业 | 241篇 |
水利工程 | 19篇 |
石油天然气 | 5篇 |
无线电 | 155篇 |
一般工业技术 | 417篇 |
冶金工业 | 119篇 |
原子能技术 | 12篇 |
自动化技术 | 441篇 |
出版年
2024年 | 3篇 |
2023年 | 29篇 |
2022年 | 75篇 |
2021年 | 95篇 |
2020年 | 69篇 |
2019年 | 71篇 |
2018年 | 88篇 |
2017年 | 71篇 |
2016年 | 114篇 |
2015年 | 84篇 |
2014年 | 107篇 |
2013年 | 186篇 |
2012年 | 176篇 |
2011年 | 183篇 |
2010年 | 122篇 |
2009年 | 90篇 |
2008年 | 104篇 |
2007年 | 92篇 |
2006年 | 72篇 |
2005年 | 49篇 |
2004年 | 53篇 |
2003年 | 29篇 |
2002年 | 50篇 |
2001年 | 25篇 |
2000年 | 11篇 |
1999年 | 8篇 |
1998年 | 26篇 |
1997年 | 16篇 |
1996年 | 22篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 8篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1988年 | 4篇 |
1987年 | 6篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1978年 | 2篇 |
1977年 | 5篇 |
1975年 | 1篇 |
1972年 | 2篇 |
1971年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有2196条查询结果,搜索用时 31 毫秒
101.
Carlo Matera Pablo Calv Vernica Casad-Anguera Rosalba Sortino Alexandre M. J. Gomila Estefanía Moreno Thomas Gener Cristina Delgado-Sallent Pau Nebot Davide Costazza Sara Conde-Berriozabal Merc Masana Jordi Hernando Vicent Casad M. Victoria Puig Pau Gorostiza 《International journal of molecular sciences》2022,23(17)
Understanding the dopaminergic system is a priority in neurobiology and neuropharmacology. Dopamine receptors are involved in the modulation of fundamental physiological functions, and dysregulation of dopaminergic transmission is associated with major neurological disorders. However, the available tools to dissect the endogenous dopaminergic circuits have limited specificity, reversibility, resolution, or require genetic manipulation. Here, we introduce azodopa, a novel photoswitchable ligand that enables reversible spatiotemporal control of dopaminergic transmission. We demonstrate that azodopa activates D1-like receptors in vitro in a light-dependent manner. Moreover, it enables reversibly photocontrolling zebrafish motility on a timescale of seconds and allows separating the retinal component of dopaminergic neurotransmission. Azodopa increases the overall neural activity in the cortex of anesthetized mice and displays illumination-dependent activity in individual cells. Azodopa is the first photoswitchable dopamine agonist with demonstrated efficacy in wild-type animals and opens the way to remotely controlling dopaminergic neurotransmission for fundamental and therapeutic purposes. 相似文献
102.
Super-resolution land cover mapping with indicator geostatistics 总被引:3,自引:0,他引:3
Many satellite images have a coarser spatial resolution than the extent of land cover patterns on the ground, leading to mixed pixels whose composite spectral response consists of responses from multiple land cover classes. Spectral unmixing procedures only determine the fractions of such classes within a coarse pixel without locating them in space. Super-resolution or sub-pixel mapping aims at providing a fine resolution map of class labels, one that displays realistic spatial structure (without artifact discontinuities) and reproduces the coarse resolution fractions. In this paper, existing approaches for super-resolution mapping are placed within an inverse problem framework, and a geostatistical method is proposed for generating alternative synthetic land cover maps at the fine (target) spatial resolution; these super-resolution realizations are consistent with all the information available.More precisely, indicator coKriging is used to approximate the probability that a pixel at the fine spatial resolution belongs to a particular class, given the coarse resolution fractions and (if available) a sparse set of class labels at some informed fine pixels. Such Kriging-derived probabilities are used in sequential indicator simulation to generate synthetic maps of class labels at the fine resolution pixels. This non-iterative and fast simulation procedure yields alternative super-resolution land cover maps that reproduce: (i) the observed coarse fractions, (ii) the fine resolution class labels that might be available, and (iii) the prior structural information encapsulated in a set of indicator variogram models at the fine resolution. A case study is provided to illustrate the proposed methodology using Landsat TM data from SE China. 相似文献
103.
Sighild Lemarchant Mlissa Sourioux Juliette Le Douce Alexandre Henriques Noëlle Callizot Sandrine Hugues Mlissa Farinelli Yann Godfrin 《International journal of molecular sciences》2022,23(16)
NX210c is a disease-modifying dodecapeptide derived from the subcommissural organ-spondin that is under preclinical and clinical development for the treatment of neurological disorders. Here, using whole-cell patch-clamp recordings, we demonstrate that NX210c increased α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)- and GluN2A-containing N-methyl-D-aspartate receptor (GluN2A-NMDAR)-mediated excitatory postsynaptic currents in the brain. Accordingly, using extracellular field excitatory postsynaptic potential recordings, an enhancement of synaptic transmission was shown in the presence of NX210c in two different neuronal circuits. Furthermore, the modulation of synaptic transmission and GluN2A-NMDAR-driven signaling by NX210c restored memory in mice chronically treated with the NMDAR antagonist phencyclidine. Overall, by promoting glutamatergic receptor-related neurotransmission and signaling, NX210c represents an innovative therapeutic opportunity for patients suffering from CNS disorders, injuries, and states with crippling synaptic dysfunctions. 相似文献
104.
105.
106.
Ferracin LM Fier CB Vieira ML Monteiro-Vitorello CB Varani Ade M Rossi MM Müller-Santos M Taniwaki MH Thie Iamanaka B Fungaro MH 《International journal of food microbiology》2012,155(3):137-145
In silico comparison of 34 putative pks genes in Aspergillus niger strain CBS 513.88 versus A. niger strain ATCC 1015 genome revealed significant nucleotide identity (>95% covering a minimum of 99% of the gene sequence) for 31 of these genes (approximately 91%). A. niger CBS 513.88 harbors three putative pks genes (An01g01130, An11g05940, and An15g07920), for which nucleotide identity was not found in A. niger ATCC 1015. To compare the results of the in silico analysis with the in vivo situation, experimental data were obtained for a large number of A. niger strains obtained from different substrates and geographical regions. Three putative pks genes that were found to be variable between the two A. niger strains using bioinformatics tools were in fact strain-specific genes based on experimental data. The PCR amplification signals for the An01g01130, An11g05940, and An15g07920 pks genes were detected in only 97%, 71%, and 26% of the strains, respectively. Southern blot analyses confirmed the PCR data. Because one of the strain-specific pks genes (An15g07920) is located in a putative ochratoxin cluster, we focused our investigation on that region. We assessed the ochratoxin production capability of the 119 A. niger strains and found a positive association between the presence of this pks gene and the capability of the respective strain to produce ochratoxin. 相似文献
107.
Pinon A Alexandre V Cupferman S Crozier A Vialette M 《International journal of cosmetic science》2007,29(2):111-119
The influence of ethanol on the behaviour of Pseudomonas aeruginosa and Staphylococcus aureus strains was evaluated throughout this study. Strains of different origin were used: collection, clinical and industrial strains were selected. Concentrations of ethanol from 0 to 20% (v/v) were evaluated by automated optical density measurements and by enumeration. When growth conditions were observed, predictive microbiology models were used to assess quantitatively for the ethanol effect. Primary modelling of kinetics was performed to determine growth rate values; secondary modelling was performed on these growth rates as influenced by ethanol, and minimum inhibitory concentrations of ethanol were determined for each strain. Staphylococcus aureus strains were more resistant to ethanol than P. aeruginosa strains, in growth conditions as well as in inactivation conditions. Furthermore, clinical S. aureus strains were more resistant than the collection strain. The method was promising for management of microbiological safety in cosmetics. 相似文献
108.
Maria Eliza de Castro Moreira Rosemary Gualberto Fonseca Alvarenga Pereira Danielle Ferreira Dias Vanessa Silva Gontijo Fabiana Cardoso Vilela Gabriel de Oliveira Isac de Moraes Alexandre Giusti-Paiva Marcelo Henrique dos Santos 《Journal of Functional Foods》2013,5(1):466-474
Green coffee contains a large quantity and variety of polyphenols and flavonoids. The roasting affects the composition of the polyphenols in coffee, due to the formation of compounds generated by Maillard reaction, which can have anti-inflammatory or antioxidant potential. The anti-inflammatory and antioxidant effects of aqueous extracts of green (AGCa) and roasted (ARCa) coffee beans were investigated in animal models and using a DPPH radical scavenging test. In the formalin test the extracts reduced licking activity only in the late phase. The inhibitory values of oedema at 3 h post-carrageenan were 53% and 74% for 100 and 300 mg/kg of the AGCa extract and 36% for ARCa (300 mg/kg). Leukocyte recruitment into the peritoneal cavity was inhibited by the extracts. The antioxidant activities of the extracts were higher than the reference antioxidants, ascorbic acid and butylated hydroxytoluene. These results indicate that coffee extracts exhibit anti-inflammatory and antioxidant properties. 相似文献
109.
Farah Perveen Mughal Ann Christina Bergmann Ha Uyen Buu Huynh Sarah Hyllekvist Jrgensen Inaam Mansha Meliha Kesmez Patrick Mark Schürch Alexandre Pierre Andr Theocharides Paul Robert Hansen Tina Friis Morten Orebo Holmstrm Evaldas Ciplys Rimantas Slibinskas Peter Hjrup Gunnar Houen Nicole Hartwig Trier 《International journal of molecular sciences》2022,23(12)
Myeloproliferative Neoplasms (MPNs) constitute a group of rare blood cancers that are characterized by mutations in bone marrow stem cells leading to the overproduction of erythrocytes, leukocytes, and thrombocytes. Mutations in calreticulin (CRT) genes may initiate MPNs, causing a novel variable polybasic stretch terminating in a common C-terminal sequence in the frameshifted CRT (CRTfs) proteins. Peptide antibodies to the mutated C-terminal are important reagents for research in the molecular mechanisms of MPNs and for the development of new diagnostic assays and therapies. In this study, eight peptide antibodies targeting the C-terminal of CRTfs were produced and characterised by modified enzyme-linked immunosorbent assays using resin-bound peptides. The antibodies reacted to two epitopes: CREACLQGWTE for SSI-HYB 385-01, 385-02, 385-03, 385-04, 385-07, 385-08, and 385-09 and CLQGWT for SSI-HYB 385-06. For the majority of antibodies, the residues Cys1, Trp9, and Glu11 were essential for reactivity. SSI-HYB 385-06, with the highest affinity, recognised recombinant CRTfs produced in yeast and the MARIMO cell line expressing CRTfs when examined in Western immunoblotting. Moreover, SSI-HYB 385-06 occasionally reacted to CRTfs from MPN patients when analysed by flow cytometry. The characterized antibodies may be used to understand the role of CRTfs in the pathogenesis of MPNs and to design and develop new diagnostic assays and therapeutic targets. 相似文献
110.
Lopez Daniel Andres Sanchez Chagas Luciana Gomes Batista Aline Domingues Guaita Maria Gabriella Detone Amorin Luís Henrique Cardozo da Silva Paulo Rogério Catarini Yamanishi Gustavo Zaia Dimas Augusto Morozin de Santana Henrique Urbano Alexandre 《Journal of Materials Science: Materials in Electronics》2021,32(13):17462-17472
Journal of Materials Science: Materials in Electronics - The increasing demand for lithium-ion batteries has stimulated the investigation of new compounds in order to reduce the costs and the... 相似文献