Anticoagulant, Antioxidant and Antitumor Activities of Heterofucans from the Seaweed Dictyopteris delicatula

In the present study, six families of sulfated polysaccharides were obtained from seaweed Dictyopteris delicatula by proteolytic digestion, followed by acetone fractionation and molecular sieving on Sephadex G-100. Chemical analyses demonstrated that all polysaccharides contain heterofucans composed mainly of fucose, xylose, glucose, galactose, uronic acid, and sulfate. The fucans F0.5v and F0.7v at 1.0 mg/mL showed high ferric chelating activity (～45%), whereas fucans F1.3v (0.5 mg/mL) showed considerable reducing power, about 53.2% of the activity of vitamin C. The fucan F1.5v presented the most prominent anticoagulant activity. The best antiproliferative activity was found with fucans F1.3v and F0.7v. However, F1.3v activity was much higher than F0.7v inhibiting almost 100% of HeLa cell proliferation. These fucans have been selected for further studies on structural characterization as well as in vivo experiments, which are already in progress.     

Experimental thermal–hydraulic evaluation of CuO nanofluids in microchannels at various concentrations with and without suspension enhancers

This experimental study focuses on the effect of two important factors on the heat transfer and flow properties of copper oxide (CuO)/water nanofluids in a parallel microchannel flow configuration. The first factor considered is the solid media (CuO) concentration. In this investigation, concentration values of 0.005%, 0.01%, and 0.1% by volume were tested. The second factor is the use of a surfactant, cetyltrimethylammonium bromide (CTAB), as a suspension enhancer. All together, these two factors led to a total of six types of nanofluids, which were tested in addition to pure water, the reference fluid. The experimental setup allowed for the determination of the hydrodynamic and thermal performance of each nanofluid. In addition, a selection of the nanofluids were characterized by the use of scanning transmission electron microscopy (STEM) and Dynamic Light Scattering (DLS) techniques. The DLS transient settling measurements showed that for a nanofluid with a concentration of 0.1% by volume, the nanoparticle dispersion and suspension is negatively affected unless a surfactant is used. Hydrodynamic losses, which were evaluated by comparing the effect of the imposed pressure drop on the mass flow rate, were not meaningfully affected by the composition of the nanofluids tested. The measurements also showed that nanofluids containing a surfactant generally provided a modest increase in heat transfer rate when compared with tests performed using pure water. The largest increase was about 17% for a fluid with a concentration of 0.01% by volume. Consequently, the gains in heat transfer do not appear to be accompanied by a significant pumping power penalty. The results of this study suggest that the use of a surfactant is essential in maintaining a proper suspension of nanoparticles in the fluid, especially at higher concentrations.     

A Simple Compressive Sensing Algorithm for Parallel Many-Core Architectures

In this paper we consider the l ¹-compressive sensing problem. We propose an algorithm specifically designed to take advantage of shared memory, vectorized, parallel and many-core microprocessors such as the Cell processor, new generation Graphics Processing Units (GPUs) and standard vectorized multi-core processors (e.g. quad-core CPUs). Besides its implementation is easy. We also give evidence of the efficiency of our approach and compare the algorithm on the three platforms, thus exhibiting pros and cons for each of them.     

On the modeling and simulation of non-hydrostatic dam break flows

The numerical simulation of three-dimensional dam break flows is discussed. A non-hydrostatic numerical model for free-surface flows is considered, which is based on the incompressible Navier–Stokes equations coupled with a volume-of-fluid approach. The numerical results obtained for a variety of benchmark problems show the validity of the numerical approach, in comparison with other numerical models, and allow to investigate numerically the non-hydrostatic three-dimensional effects, in particular for the usual test cases where hydrostatic approximations are known analytically. The numerical experiments on actual topographies, in particular the Malpasset dam break and the (hypothetical) break of the Grande-Dixence dam in Switzerland, also illustrate the capabilities of the method for large-scale simulations and real-life visualization.     

Considering complex training images with search tree partitioning

Using a complex training image (TI) for the single normal equation simulation (SNESIM) algorithm results in poor simulated realizations since that image contains trends and location specific patterns. By pooling all the TI patterns in a single search tree and not recording the relative locations of those patterns, some critical features of these complex TIs are lost.The search tree partitioning approach subdivides a large TI into imbricated, homogeneous, smaller images, called partition classes. Each of these partition classes has a corresponding search tree that can be utilized by the SNESIM algorithm. These partition classes are obtained by processing the TIs with spatial filters that are pattern sensitive. The resulting filter scores are then clustered into partition classes. All patterns within a partition class are recorded by a search tree; there is one tree per partition class. At each pixel along the simulation path, the partition class is retrieved first and used to select the appropriate search tree. That search tree contains the patterns relevant to that partition class.In practice, the partitioning approach adds flexibility in choosing a TI. TIs that were easier to obtain but traditionally too complex for simulation can now be considered as input to SNESIM. In many cases, it also significantly increases the simulation speed by searching a vector of smaller trees instead of a single large one. A plugin for the SGeMS software is provided.     

Forecast of mycotoxins levels in soft wheat, durum wheat and maize before harvesting with Qualim��tre?

Some mycotoxins are produced by several Fusarium species during cultivation and are found in wheat and maize grain. Since 2000, Syngenta has organised a large field survey. Agronomic and climatic data and grain samples have been collected for mycotoxin analysis in France and Belgium. The importance of the agroclimatic factors and their interactions on the mycotoxin levels in grain has been estimated. The climate around flowering stage is the major factor for deoxynivalenol (DON) in wheat. The main agronomic criteria are residue management and the variety sensitivity to this mycotoxin. For DON, zearalenone and fumonisins in maize, the climate from flowering stage until harvest is the major factor. Then, according to each mycotoxin, the main agronomic criteria are the harvest condition (date and grain moisture), the corn borer infestation and the variety sensitivity to these mycotoxins. Over the years, the database has been used to define models to predict the mycotoxin risk before harvesting. Grain purchasers enter the required agronomic data via the Syngenta Internet site and define their grain purchasing areas. They also define the flowering period for wheat and corn borer infestation for maize. After calculation which integrates climatic data, the purchasers receive reports with forecasts of mycotoxin levels. Prediction is based on different agro-climatic statistical models specifically configured according to the different regions of production in France and Belgium. This approach is called Qualimètre^? and was the first service in France and Belgium to forecast the grain mycotoxin level for wheat in 2004 and maize in 2006.     

Comparison of the wound-activated transformation of caulerpenyne by invasive and noninvasive Caulerpa species of the Mediterranean

The invasive green alga, Caulerpa taxifolia, that has spread rapidly after its introduction into the Mediterranean and the North American Pacific, reacts to wounding by transforming its major metabolite caulerpenyne (1). This wound-activated reaction involves the transformation of the bis-enol acetate moiety of 1, releasing reactive 1,4-dialdehydes. The ability to perform this transformation is found also in both the noninvasive Mediterranean C. prolifera and the invasive C. racemosa. Trapping experiments, as well as transformation of the model substrate geranyl acetate, suggest that all three investigated Caulerpa spp. rely on esterases that act upon wounding of the algae by subsequently removing the three acetate residues of caulerpenyne. The resulting reactive 1,4-dialdehyde oxytoxin 2 (9) can be identified by liquid chromatography–mass spectrometry and is unstable in the wounded tissue. Caulerpenyne transformation occurs rapidly, and severe tissue damage caused degradation of more than 50% of the stored caulerpenyne within 1 min in all three algae. Prevention of the enzymatic reaction before extraction, by shock freezing the tissue with liquid nitrogen, was used for the determination of the caulerpenyne content in intact algae. It gives about twofold higher values compared to an established methanol extraction protocol. The speed and mechanism of the wound-activated transformation, as well as the caulerpenyne content in intact tissue of invasive and noninvasive Caulerpa spp., are comparable. Thus, this enzymatic , transformation, despite being fast and efficient, is likely not the key for the success of the investigated invasive species.     

Stopped flow kinetic-spectrophotometric determination of Diquat in waters

Using the measures of initial reaction rate, a kinetic-photometric method is developed that uses the reaction of Diquat with H2O2 in basic medium. The reaction rate is very high and therefore the stopped-flow mixing technique is required in order to make kinetic measurements. The reaction rate is obtained in 20 s after the reactants are mixed and it allows the simple application of the method proposed to the routine analyses. The sensitivity of the method is very high being the detection limit 0.44 microgmL(-1) and the application range between 1 and 16 microgmL(-1). The time of measure per sample is 90s. The proposed kinetic-photometric method has been applied to the direct determination of this compound in river water, in the water of laundry of the olives, and in a commercial product. The results are very acceptable.     

A unified signal transition graph model for asynchronous control circuit synthesis

Characterization of the behavior of an asynchronous system depending on the delay of components and wires is a major task facing designers. Some of these delays are outside the designer's control, and in practice may have to be assumed unbounded. The existing literature offers a number of analysis and specification models, but lacks a unified framework to verify directly if the circuit specification admits a correct implementation under these hypotheses.Our aim is to fill exactly this gap, offering both low-level (analysis-oriented) and high-level (specification-oriented) models for asynchronous circuits and the environment where they operate, together with strong equivalence results between the properties at the two levels. One interesting side result is the precise characterization of classical static and dynamic hazards in terms of our model. Consequently the designer can check the specification and directly decide if the behavior of any implementation will depend, e.g., on the delays of the signals described by such specification.We also outline a design methodology based on our models, pointing out how they can be used to select appropriate high and low-level models depending on the desired characteristics of the system.     

Immune response to baculovirus expressed protein fragment amino acids 190-289 of respiratory syncytial virus (RSV) fusion protein

At least two neutralizing epitopes have been identified in the amino acid (aa) sequence 190-289 of the RSV fusion protein. The authors expressed this region in insect cells (bF190-289) and compared the immune response to bF190-289 with that induced by baculovirus expressed full-length fusion protein (bF). As with bF, mice primed with bF190-289 produced exclusively antibodies of IgG1 isotype, generated neutralizing antibodies, reduced significantly the virus titer (about a half log10 reduction) after RSV challenge and induced a Helper T (Th) 2 cell response in mediastinal lymph node cells (MLNC) restimulated in vitro. Thus, the aa sequence 190-289 represents a major immunogenic region of the RSV fusion protein.