全文获取类型
| 收费全文 | 1660篇 |
| 免费 | 20篇 |
专业分类
| 电工技术 | 19篇 |
| 化学工业 | 378篇 |
| 金属工艺 | 21篇 |
| 机械仪表 | 28篇 |
| 建筑科学 | 76篇 |
| 矿业工程 | 3篇 |
| 能源动力 | 61篇 |
| 轻工业 | 200篇 |
| 水利工程 | 8篇 |
| 石油天然气 | 4篇 |
| 无线电 | 116篇 |
| 一般工业技术 | 205篇 |
| 冶金工业 | 412篇 |
| 原子能技术 | 12篇 |
| 自动化技术 | 137篇 |
出版年
| 2024年 | 18篇 |
| 2023年 | 23篇 |
| 2022年 | 71篇 |
| 2021年 | 81篇 |
| 2020年 | 34篇 |
| 2019年 | 35篇 |
| 2018年 | 35篇 |
| 2017年 | 39篇 |
| 2016年 | 42篇 |
| 2015年 | 20篇 |
| 2014年 | 40篇 |
| 2013年 | 79篇 |
| 2012年 | 75篇 |
| 2011年 | 66篇 |
| 2010年 | 64篇 |
| 2009年 | 72篇 |
| 2008年 | 78篇 |
| 2007年 | 50篇 |
| 2006年 | 33篇 |
| 2005年 | 31篇 |
| 2004年 | 38篇 |
| 2003年 | 34篇 |
| 2002年 | 36篇 |
| 2001年 | 27篇 |
| 2000年 | 17篇 |
| 1999年 | 27篇 |
| 1998年 | 91篇 |
| 1997年 | 51篇 |
| 1996年 | 45篇 |
| 1995年 | 36篇 |
| 1994年 | 30篇 |
| 1993年 | 27篇 |
| 1992年 | 27篇 |
| 1991年 | 17篇 |
| 1990年 | 15篇 |
| 1989年 | 11篇 |
| 1988年 | 12篇 |
| 1987年 | 18篇 |
| 1986年 | 14篇 |
| 1985年 | 14篇 |
| 1984年 | 13篇 |
| 1983年 | 8篇 |
| 1982年 | 8篇 |
| 1981年 | 5篇 |
| 1980年 | 10篇 |
| 1979年 | 6篇 |
| 1978年 | 8篇 |
| 1976年 | 17篇 |
| 1974年 | 6篇 |
| 1970年 | 4篇 |
排序方式： 共有1680条查询结果，搜索用时 15 毫秒
991.
Aaron A. Shinkle Alice E. S. Sleightholme Levi T. Thompson Charles W. Monroe 《Journal of Applied Electrochemistry》2011,41(10):1191-1199
Three electrode materials (glassy carbon, gold, and platinum) were investigated for application in a non-aqueous single-metal
redox flow battery based on vanadium (III) acetylacetonate, supported by tetraethylammonium tetrafluoroborate in acetonitrile.
Redox couples associated with the one-electron disproportionation of V(acac)3 were observed in voltammetry for each metal tested. An elementary kinetic model was created and used to determine rates for
oxidation or reduction of the vanadium complex. The oxidation rates for V(acac)3 were mass-transfer limited on all electrode materials, suggesting reversible kinetics. For the V(acac)3 reduction reaction, exchange-current densities of 1.3, 3.8, and 8.4 A m−2 were observed on glassy carbon, platinum, and gold electrodes, respectively. 相似文献
992.
993.
Alice Barinotti Massimo Radin Irene Cecchi Silvia Grazietta Foddai Elena Rubini Dario Roccatello Savino Sciascia Elisa Menegatti 《International journal of molecular sciences》2020,21(24)
As in many autoimmune diseases, the pathogenesis of the antiphospholipid syndrome (APS) is the result of a complex interplay between predisposing genes and triggering environmental factors, leading to a loss of self-tolerance and immune-mediated tissue damage. While the first genetic studies in APS focused primarily on the human leukocytes antigen system (HLA) region, more recent data highlighted the role of other genes in APS susceptibility, including those involved in the immune response and in the hemostatic process. In order to join this intriguing debate, we analyzed the single-nucleotide polymorphisms (SNPs) derived from the whole exome sequencing (WES) of two siblings affected by APS and compared our findings with the available literature. We identified genes encoding proteins involved in the hemostatic process, the immune response, and the phospholipid metabolism (PLA2G6, HSPG2, BCL3, ZFAT, ATP2B2, CRTC3, and ADCY3) of potential interest when debating the pathogenesis of the syndrome. The study of the selected SNPs in a larger cohort of APS patients and the integration of WES results with the network-based approaches will help decipher the genetic risk factors involved in the diverse clinical features of APS. 相似文献
994.
995.
Alice Indini Erika Rijavec Michele Ghidini Gianluca Tomasello Monica Cattaneo Francesca Barbin Claudia Bareggi Barbara Galassi Donatella Gambini Francesco Grossi 《International journal of molecular sciences》2021,22(5)
Growing research has focused on obesity as a prognostic factor during therapy with immune-checkpoint inhibitors (ICIs). The role of body-mass index (BMI) in predicting response and toxicity to ICIs is not clear, as studies have shown inconsistent results and significant interpretation biases. We performed a systematic review to evaluate the relationship between BMI and survival outcomes during ICIs, with a side focus on the incidence of immune-related adverse events (irAEs). A total of 17 studies were included in this systematic review. Altogether, the current evidence does not support a clearly positive association of BMI with survival outcomes. Regarding toxicities, available studies confirm a superimposable rate of irAEs among obese and normal weight patients. Intrinsic limitations of the analyzed studies include the retrospective nature, the heterogeneity of patients’ cohorts, and differences in BMI categorization for obese patients across different studies. These factors might explain the heterogeneity of available results, and the subsequent absence of a well-established role of baseline BMI on the efficacy of ICIs among cancer patients. Further prospective studies are needed, in order to clarify the role of obesity in cancer patients treated with immunotherapy. 相似文献
996.
Giovanni Bacci Alice Rossi Federica Armanini Lisa Cangioli Ida De Fino Nicola Segata Alessio Mengoni Alessandra Bragonzi Annamaria Bevivino 《International journal of molecular sciences》2021,22(22)
Cystic fibrosis (CF) disease leads to altered lung and gut microbiomes compared to healthy subjects. The magnitude of this dysbiosis is influenced by organ-specific microenvironmental conditions at different stages of the disease. However, how this gut-lung dysbiosis is influenced by Pseudomonas aeruginosa chronic infection is unclear. To test the relationship between CFTR dysfunction and gut-lung microbiome under chronic infection, we established a model of P. aeruginosa infection in wild-type (WT) and gut-corrected CF mice. Using 16S ribosomal RNA gene, we compared lung, stool, and gut microbiota of C57Bl/6 Cftr tm1UNCTgN(FABPCFTR) or WT mice at the naïve state or infected with P. aeruginosa. P. aeruginosa infection influences murine health significantly changing body weight both in CF and WT mice. Both stool and gut microbiota revealed significantly higher values of alpha diversity in WT mice than in CF mice, while lung microbiota showed similar values. Infection with P. aeruginosa did not changed the diversity of the stool and gut microbiota, while a drop of diversity of the lung microbiota was observed compared to non-infected mice. However, the taxonomic composition of gut microbiota was shown to be influenced by P. aeruginosa infection in CF mice but not in WT mice. This finding indicates that P. aeruginosa chronic infection has a major impact on microbiota diversity and composition in the lung. In the gut, CFTR genotype and P. aeruginosa infection affected the overall diversity and taxonomic microbiota composition, respectively. Overall, our results suggest a cross-talk between lung and gut microbiota in relation to P. aeruginosa chronic infection and CFTR mutation. 相似文献
997.
Alice Abend Chelsie Steele Heinz-Georg Jahnke Mareike Zink 《International journal of molecular sciences》2021,22(16)
Coupling of cells to biomaterials is a prerequisite for most biomedical applications; e.g., neuroelectrodes can only stimulate brain tissue in vivo if the electric signal is transferred to neurons attached to the electrodes’ surface. Besides, cell survival in vitro also depends on the interaction of cells with the underlying substrate materials; in vitro assays such as multielectrode arrays determine cellular behavior by electrical coupling to the adherent cells. In our study, we investigated the interaction of neurons and glial cells with different electrode materials such as TiN and nanocolumnar TiN surfaces in contrast to gold and ITO substrates. Employing single-cell force spectroscopy, we quantified short-term interaction forces between neuron-like cells (SH-SY5Y cells) and glial cells (U-87 MG cells) for the different materials and contact times. Additionally, results were compared to the spreading dynamics of cells for different culture times as a function of the underlying substrate. The adhesion behavior of glial cells was almost independent of the biomaterial and the maximum growth areas were already seen after one day; however, adhesion dynamics of neurons relied on culture material and time. Neurons spread much better on TiN and nanocolumnar TiN and also formed more neurites after three days in culture. Our designed nanocolumnar TiN offers the possibility for building miniaturized microelectrode arrays for impedance spectroscopy without losing detection sensitivity due to a lowered self-impedance of the electrode. Hence, our results show that this biomaterial promotes adhesion and spreading of neurons and glial cells, which are important for many biomedical applications in vitro and in vivo. 相似文献
998.
Daniel L. Pouliquen Alice Boissard Ccile Henry Stphanie Blandin Olivier Coqueret Catherine Guette 《International journal of molecular sciences》2021,22(16)
This study aimed to identify the proteomic changes produced by curcumin treatment following stimulation of the host immune system in a rat model of malignant mesothelioma. We analyzed the proteomes of secondary lymphoid organs from four normal rats, four untreated tumor-bearing rats, and four tumor-bearing rats receiving repeated intraperitoneal administrations of curcumin. Cross-comparing proteome analyses of histological sections of the spleen from the three groups first identified a list of eighty-three biomarkers of interest, thirteen of which corresponded to proteins already reported in the literature and involved in the anticancer therapeutic effects of curcumin. In a second step, comparing these data with proteomic analyses of histological sections of mesenteric lymph nodes revealed eight common biomarkers showing a similar pattern of changes in both lymphoid organs. Additional findings included a partial reduction of the increase in spleen-circulating biomarkers, a decrease in C-reactive protein and complement C3 in the spleen and lymph nodes, and an increase in lymph node purine nucleoside phosphorylase previously associated with liver immunodeficiency. Our results suggest some protein abundance changes could be related to the systemic, distant non-target antitumor effects produced by this phytochemical. 相似文献
999.
Samantha D. Francis Stuart Alice R. Villalobos 《International journal of molecular sciences》2021,22(16)
Choroid plexus (CP) sequesters cadmium and other metals, protecting the brain from these neurotoxins. These metals can induce cellular stress and modulate homeostatic functions of CP, such as solute transport. We previously showed in primary cultured neonatal rat CP epithelial cells (CPECs) that cadmium induced cellular stress and stimulated choline uptake at the apical membrane, which interfaces with cerebrospinal fluid in situ. Here, in CPECs, we characterized the roles of glutathione (GSH) and Zinc supplementation in the adaptive stress response to cadmium. Cadmium increased GSH and decreased the reduced GSH-to-oxidized GSH (GSSG) ratio. Heat shock protein-70 (Hsp70), heme oxygenase (HO-1), and metallothionein (Mt-1) were induced along with the catalytic and modifier subunits of glutamate cysteine ligase (GCL), the rate-limiting enzyme in GSH synthesis. Inhibition of GCL by l-buthionine sulfoximine (BSO) enhanced stress protein induction and stimulation of choline uptake by cadmium. Zinc alone did not induce Hsp70, HO-1, or GCL subunits, or modulate choline uptake. Zinc supplementation during cadmium exposure attenuated stress protein induction and stimulation of choline uptake; this effect persisted despite inhibition of GSH synthesis. These data indicated up-regulation of GSH synthesis promotes adaptation to cadmium-induced cellular stress in CP, but Zinc may confer cytoprotection independent of GSH. 相似文献
1000.
Alice De Palo Dijana Draca Maria Grazia Murrali Stefano Zacchini Guido Pampaloni Sanja Mijatovic Danijela Maksimovic-Ivanic Fabio Marchetti 《International journal of molecular sciences》2021,22(14)
Piano-stool iridium complexes based on the pentamethylcyclopentadienyl ligand (Cp*) have been intensively investigated as anticancer drug candidates and hold much promise in this setting. A systematic study aimed at outlining the effect of Cp* mono-derivatization on the antiproliferative activity is presented here. Thus, the dinuclear complexes [Ir(η5-C5Me4R)Cl(μ-Cl)]2 (R = Me, 1a; R = H, 1b; R = Pr, 1c; R = 4-C6H4F, 1d; R = 4-C6H4OH, 1e), their 2-phenylpyridyl mononuclear derivatives [Ir(η5-C5Me4R)(kN,kCPhPy)Cl] (2a–d), and the dimethylsulfoxide complex [Ir{η5-C5Me4(4-C6H4OH)}Cl2(κS-Me2S=O)] (3) were synthesized, structurally characterized, and assessed for their cytotoxicity towards a panel of six human and rodent cancer cell lines (mouse melanoma, B16; rat glioma, C6; breast adenocarcinoma, MCF-7; colorectal carcinoma, SW620 and HCT116; ovarian carcinoma, A2780) and one primary, human fetal lung fibroblast cell line (MRC5). Complexes 2b (R = H) and 2d (4-C6H4F) emerged as the most active ones and were selected for further investigation. They did not affect the viability of primary mouse peritoneal cells, and their tumoricidal action arises from the combined influence on cellular proliferation, apoptosis and senescence. The latter is triggered by mitochondrial failure and production of reactive oxygen and nitrogen species. 相似文献