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21.
Nicoleta Stănciuc Loredana Dumitraşcu Alina Ardelean Silvius Stanciu Gabriela Râpeanu 《Food and Bioprocess Technology》2012,5(6):2160-2171
Whey protein concentrate (WPC) is used as food ingredients due to their commercially important functional properties. The effects of heat treatment on the components of milk are very important for the final product character, since they undergo modifications that affect sensorial and nutritional quality of milk. The heat-induced changes on dispersions of whey proteins concentrate were monitored by measurement of thiol availability, protein solubility, and turbidity at pH 6.6 and 7.5. The fractional conversion model was used to quantitatively describe the effect of different temperature–time combination on denaturation mechanism. The results demonstrate that heat-induced changes of WPC greatly influence their solubility, expressed as degree of denaturation at pH 4.6 and were related to the heating conditions. The denaturation mechanism involved a number of consecutive conformational changes in the molecules. A curvature in Arrhenius plots was observed around 75 °C, indicating changes in the reaction mechanism. The deflection of Arrhenius plot reflects the generally accepted two-step denaturation/aggregation process of whey proteins. 相似文献
22.
Jane Turner John E. Snawder Alina Handorean Kevin M. McCabe 《Aerosol science and technology》2013,47(8):599-610
Epidemiological studies have shown that exposure to airborne particulate matter (PM) can be an important risk factor for some common respiratory diseases. While many studies have shown that PM exposures are associated with inflammatory reactions, the role of specific cellular responses in the manifestation of primary hypersensitivities and the progression of respiratory diseases remains unclear. In order to better understand mechanisms by which PM can exert adverse health effects, more robust approaches to support in vitro studies are warranted. In response to this need, a group of accepted toxicology assays was adapted to create an analytical suite for screening and evaluating the effects of important, ubiquitous atmospheric pollutants on two model human lung cell lines (epithelial and immature macrophage). To demonstrate the utility of this suite, responses to intact diesel exhaust particles (DEP) and mass-based equivalent doses of their organic extracts were examined. Results suggest that extracts have the potential to induce greater biological responses than those associated with their colloidal counterpart. Additionally, macrophage cells appear to be more susceptible to the cytotoxic effects of both intact DEP and their organic extract, than epithelial cells tested in parallel. As designed, the suite provided a more robust basis for characterizing toxicity mechanisms than the analysis of any individual assay. Findings suggest that cellular responses to PM are cell line dependent, and show that the collection and preparation of PM and/or their extracts have the potential to impact cellular responses relevant to screening fundamental elements of respiratory toxicity.Copyright 2015 American Association for Aerosol Research 相似文献
23.
Andreea Groza Simona Liliana Iconaru Gabriel Jiga Patrick Chapon Sofia Gaiaschi Nicolae Verga Mircea Beuran Alina Mihaela Prodan Mihai Matei Serban Andrei Marinescu Roxana Trusca Daniela Predoi 《Polymer Engineering and Science》2019,59(12):2406-2412
The bio hydroxyapatite (HAp) was used from a long time in different medical and environmental applications. The HAp layers with a uniform surface were used for various medical applications such as orthopedic and dental metal implants. In this work, we reported on the influence of X‐ray radiation on the structural and morphological properties of composite layers based on HAp and polydimethylsiloxane (PDMS) deposited on titanium substrates. The HAp:PDMS layers were investigated by different complementary methods such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and glow discharge optical emission spectrometry (GDOES). FTIR spectral analysis showed that the molecular structure of the coatings was not changed after their irradiation even though, the depth profile analysis performed by GDOES indicated a depletion of Ca and P elements from the HAp:PDMS irradiated samples. By SEM, we showed that the morphological features of the coatings were also changed, as the irradiated layers are delaminated. The biological assays confirmed that the antibacterial activity of HAp:PDMS composite layers increased after irradiation. The results obtained in this study highlighted that the biological properties of HAp:PDMS layers could be influenced by irradiation. POLYM. ENG. SCI., 59:2406–2412, 2019. © 2019 Society of Plastics Engineers 相似文献
24.
Alexander Filippov Alina I Amirova Tatiana Kirila Elena V Belyaeva Natalia A Sheremetyeva Aziz M Muzafarov 《Polymer International》2015,64(6):780-786
A carbosilane pseudo‐dendrimer with irregular structure and degree of branching DB = 1 was synthesized and fractionated. The molar masses of the obtained fractions ranged from 5000 to 490 000 g mol?1. The hydrodynamic and conformational properties were studied by the methods of light scattering, sedimentation‐diffusion analysis and viscometry in dilute hexane and toluene solutions. The Mark ? Kuhn ? Houwink dependences were obtained. The results for the pseudo‐dendrimer are compared with the data for the initial hyperbranched poly(diallylcarbosilane) with DB = 0.5 and carbosilane dendrimer. It is shown that the solution properties of the pseudo‐dendrimer are close to those for the irregular hyperbranched polymer and differ significantly from the dendrimer behavior. Hence, it is branching regularity rather than the degree of branching that determines the conformational and hydrodynamic properties of hyperbranched polymers with a high degree of branching DB ≥ 0.5. © 2014 Society of Chemical Industry 相似文献
25.
Marta Izabela Jonas Alina Kurylowicz Zbigniew Bartoszewicz Wojciech Lisik Maurycy Jonas Zbigniew Wierzbicki Andrzej Chmura Piotr Pruszczyk Monika Puzianowska-Kuznicka 《International journal of molecular sciences》2015,16(10):25817-25830
Excess adiposity is associated with chronic inflammation, which takes part in the development of obesity-related complications. The aim of this study was to establish whether subcutaneous (SAT) or visceral (VAT) adipose tissue plays a major role in synthesis of pro-inflammatory cytokines. Concentrations of interleukins (IL): 1β, 6, 8 and 15 were measured at the protein level by an ELISA-based method and on the mRNA level by real-time PCR in VAT and SAT samples obtained from 49 obese (BMI > 40 kg/m2) and 16 normal-weight (BMI 20–24.9 kg/m2) controls. IL-6 and IL-15 protein concentrations were higher in SAT than in VAT for both obese (p = 0.003 and p < 0.0001, respectively) and control individuals (p = 0.004 and p = 0.001, respectively), while for IL-1β this was observed only in obese subjects (p = 0.047). What characterized obese individuals was the higher expression of IL-6 and IL-15 at the protein level in VAT compared to normal-weight controls (p = 0.047 and p = 0.016, respectively). Additionally, obese individuals with metabolic syndrome had higher IL-1β levels in VAT than did obese individuals without this syndrome (p = 0.003). In conclusion, concentrations of some pro-inflammatory cytokines were higher in SAT than in VAT, but it was the increased pro-inflammatory activity of VAT that was associated with obesity and metabolic syndrome. 相似文献
26.
27.
Giovanni Crupi Dominique M. M.‐P. Schreurs Alina Caddemi Antonio Raffo Giorgio Vannini 《国际射频与微波计算机辅助工程杂志》2010,20(1):87-93
The present article analyzes in detail different intrinsic small‐signal models for transistors. Particular attention is devoted to the non‐quasi‐static effects, which play a crucial role at microwave and millimeter‐wave frequencies. The advantages and disadvantages of these different equivalent circuit topologies are analyzed from both theoretical and experimental standpoints. This study clearly proves that best choice among these model representations depends on the specific device technology besides the investigated frequency range. © 2009 Wiley Periodicals, Inc. Int J RF and Microwave CAE, 2010. 相似文献
28.
Hsin-Neng Wang Janna K. Register Andrew M. Fales Naveen Gandra Eugenia H. Cho Alina Boico Gregory M. Palmer Bruce Klitzman Tuan Vo-Dinh 《Nano Research》2018,11(8):4005-4016
Although nanotechnology has led to important advances in in vitro diagnostics, the development of nanosensors for in vivo detection remains very challenging. Here, we demonstrated the proof-of-principle of in vivo detection of nucleic acid targets using a promising type of surface-enhanced Raman scattering (SERS) nanosensor implanted in the skin of a large animal model (pig). The in vivo nanosensor used in this study involves the “inverse molecular sentinel” detection scheme using plasmonics-active nanostars, which have tunable absorption bands in the near infrared region of the “tissue optical window”, rendering them efficient as an optical sensing platform for in vivo optical detection. Ex vivo measurements were also performed using human skin grafts to demonstrate the detection of SERS nanosensors through tissue. In this study, a new core–shell nanorattle probe with Raman reporters trapped between the core and shell was utilized as an internal standard system for self-calibration. These results illustrate the usefulness and translational potential of the SERS nanosensor for in vivo biosensing. 相似文献
29.
Alireza Tavakkoli Mircea Nicolescu George Bebis Monica Nicolescu 《Machine Vision and Applications》2009,20(6):395-409
Most methods for foreground region detection in videos are challenged by the presence of quasi-stationary backgrounds—flickering
monitors, waving tree branches, moving water surfaces or rain. Additional difficulties are caused by camera shake or by the
presence of moving objects in every image. The contribution of this paper is to propose a scene-independent and non-parametric
modeling technique which covers most of the above scenarios. First, an adaptive statistical method, called adaptive kernel
density estimation (AKDE), is proposed as a base-line system that addresses the scene dependence issue. After investigating
its performance we introduce a novel general statistical technique, called recursive modeling (RM). The RM overcomes the weaknesses
of the AKDE in modeling slow changes in the background. The performance of the RM is evaluated asymptotically and compared
with the base-line system (AKDE). A wide range of quantitative and qualitative experiments is performed to compare the proposed
RM with the base-line system and existing algorithms. Finally, a comparison of various background modeling systems is presented
as well as a discussion on the suitability of each technique for different scenarios. 相似文献
30.
Mariana‐Dana Damaceanu Radu‐Dan Rusu Alina Nicolescu Maria Bruma Alexander L Rusanov 《Polymer International》2011,60(8):1248-1258
This study focuses on the synthesis and characterization of new polyamides based on an aromatic asymmetric diamine‐containing phenoxy‐substituted benzophenone segment. Low‐temperature solution polycondensation reactions of this diamine with various aromatic diacid chlorides containing ether, hexafluoroisopropylidene or diphenylsilane groups resulted in polyamides with molecular weights in the range 102 900–113 200 g mol?1. The structures of these monomers and the corresponding polymers were fully confirmed using elemental analysis and infrared and NMR spectroscopy. All polyamides were easily soluble at room temperature in polar aprotic solvents and even in less polar solvents such as tetrahydrofuran. The polymers showed excellent thermal stability, up to 385 °C, and displayed glass transition temperatures in the range 225–256 °C. All the polymers presented blue florescence upon irradiation with UV light and thus show promise for applications in electroluminescent devices. Copyright © 2011 Society of Chemical Industry 相似文献