Another version of the relay feedback experiment

Process dynamic behaviour is a key for designing any control scheme. A simple method to determine a point on the frequency characteristics of the plant is the closed loop relay experiment. This paper proposes a new version for this method, presenting its possible advantages, simulation and real time experimental results.     

THEORETICAL ANALYSIS OF FATIGUE CRACK GROWTH IN A COATED SUBSTRATE

A surface crack penetrating the interface between a presstressd hard coating and a substrate is analysed in terms of linear fracture mechanics in order to assess the fatigue properties of such a composite. Assuming Paris law, fatigue crack growth rate allows the determination of safe regimes, where a crack always experiences closure.     

Simultaneous continuous,nonchromatographic monitoring and discrete chromatographic monitoring of polymerization reactions

Automatic continuous nonchromatographic monitoring and discrete chromatographic monitoring were coupled together for the first time and used to monitor free radical and controlled‐radical polymerization reactions. This was achieved by adding a multidetector Size Exclusion Chromatography (SEC) system (alternatively termed Gel Permeation Chromatography, GPC) to the ACOMP platform (Automatic Continuous Online Monitoring of Polymerization reactions). The fact that the reactor solution is already preconditioned in the ACOMP front‐end to the concentration levels used in SEC makes direct coupling possible. Kinetics from two different types of reactions, Reversible Addition Fragmentation Transfer (RAFT) and free radical polymerization of butyl acrylate were studied, including the production of a bimodal population. Complementary and contrasting features from the continuous and SEC approaches are highlighted. The main advantage of the SEC detection is to follow the evolution of full molecular weight distributions (MWD), especially in ‘living’ type reactions, where polydispersity decreases with monomer conversion, whereas the continuous detection provides a much more detailed characterization of the reaction. Interestingly, in the case where a bimodal molecular weight distribution was produced, the continuous method automatically detected the onset of the second mode in a model independent fashion, whereas SEC could only discern the bimodality by applying preconceived models. The SEC approach will have valuable niche applications, however, such as when reactions are relatively slow, monitoring narrow polydispersity is of primary importance, and also in copolymerization and terpolymerization reactions where complex mixtures of reagents (e.g., RAFT agents, copper ions, etc.) make unfractionated spectroscopic resolution of comonomers difficult. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009     

The Effect of Cucurbit[7]uril on the Antitumor and Immunomodulating Properties of Oxaliplatin and Carboplatin

Cucurbit[7]uril (CB[7]) is a molecular container that may form host–guest complexes with platinum(II) anticancer drugs and modulate their efficacy and safety. In this paper, we report our studies of the effect of CB[7]–oxaliplatin complex and the mixture of CB[7] and carboplatin (1:1) on viability and proliferation of a primary cell culture (peripheral blood mononuclear cells), two tumor cell lines (B16 and K562) and their activity in the animal model of melanoma. At the same time, we studied the impact of platinum (II) drugs with CB[7] on T cells and B cells in vitro. Although the stable CB[7]–carboplatin complex was not formed, the presence of cucurbit[7]uril affected the biological properties of carboplatin. In vivo, CB[7] increased the antitumor effect of carboplatin, but, at the same time, increased its acute toxicity. Compared to free oxaliplatin, its complex with CB[7] shows a greater cytotoxic effect on tumor cell lines B16 and K562, while in vivo, the effects of the free drug and encapsulated drug were comparable. However, in vivo studies also demonstrated that the encapsulation of oxaliplatin in CB[7] lowered the toxicity of the drug.     

The Role of TRPM2 in Endothelial Function and Dysfunction

The transient receptor potential (TRP) melastatin-like subfamily member 2 (TRPM2) is a non-selective calcium-permeable cation channel. It is expressed by many mammalian tissues, including bone marrow, spleen, lungs, heart, liver, neutrophils, and endothelial cells. The best-known mechanism of TRPM2 activation is related to the binding of ADP-ribose to the nudix-box sequence motif (NUDT9-H) in the C-terminal domain of the channel. In cells, the production of ADP-ribose is a result of increased oxidative stress. In the context of endothelial function, TRPM2-dependent calcium influx seems to be particularly interesting as it participates in the regulation of barrier function, cell death, cell migration, and angiogenesis. Any impairments of these functions may result in endothelial dysfunction observed in such conditions as atherosclerosis or hypertension. Thus, TRPM2 seems to be an attractive therapeutic target for the conditions connected with the increased production of reactive oxygen species. However, before the application of TRPM2 inhibitors will be possible, some issues need to be resolved. The main issues are the lack of specificity, poor membrane permeabilization, and low stability in in vivo conditions. The article aims to summarize the latest findings on a role of TRPM2 in endothelial cells. We also show some future perspectives for the application of TRPM2 inhibitors in cardiovascular system diseases.     

The Effect of the Ionizing Radiation on Hydroxyapatite–Polydimethylsiloxane Layers

The bio hydroxyapatite (HAp) was used from a long time in different medical and environmental applications. The HAp layers with a uniform surface were used for various medical applications such as orthopedic and dental metal implants. In this work, we reported on the influence of X‐ray radiation on the structural and morphological properties of composite layers based on HAp and polydimethylsiloxane (PDMS) deposited on titanium substrates. The HAp:PDMS layers were investigated by different complementary methods such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and glow discharge optical emission spectrometry (GDOES). FTIR spectral analysis showed that the molecular structure of the coatings was not changed after their irradiation even though, the depth profile analysis performed by GDOES indicated a depletion of Ca and P elements from the HAp:PDMS irradiated samples. By SEM, we showed that the morphological features of the coatings were also changed, as the irradiated layers are delaminated. The biological assays confirmed that the antibacterial activity of HAp:PDMS composite layers increased after irradiation. The results obtained in this study highlighted that the biological properties of HAp:PDMS layers could be influenced by irradiation. POLYM. ENG. SCI., 59:2406–2412, 2019. © 2019 Society of Plastics Engineers     

Airflow inside an open ventilated system: Influence of operator’s arms or moving conveyor

This study deals with the influence of static and dynamic objects on the airflow (generated by a unidirectional air supply) inside an “open” ventilated system used in an agro-food production line. Firstly, the presence of operator’s arms, considered here as objects, leads to the appearance of recirculating zones under each arm. The influence of different positions of the static arms protected by gloves on the airflow organization has been studied. Secondly, modifications induced by the motion of a conveyor system or of moving arms on the airflow have been explored.In an experimental approach, airflow organization has been studied by visualization and by Particle Image Velocimetry for static cases and moving conveyor case.Numerical simulations were carried out using Computational Fluids Dynamics codes. Results obtained for moving gloves cases highlight the contribution of dynamic meshes tools, implemented in the CFD codes to make simulations of moving objects.     

C60 and C60-arginine aqueous solutions: In vitro toxicity and structural study

Abstract

Small-angle X-ray and neutron scattering was applied for the structural characterization of aggregates in water dispersions of fullerene C₆₀ prepared by dialysis method and its conjugate with amino acid arginine. Two compounds are also compared with respect to their toxic properties. Experiments on the cytotoxicity of these systems on the A549, HepG2 and HeLa cells showed no toxic effects of the dispersions.     

Effect of ball milling on the thermal conductivity and viscosity of Indian coal fly ash nanofluid

The present study investigates the effect of ball milling on thermal conductivity and viscosity of stable nanofluid of fly ash from Indian coal. The particle size of fly ash decreased from micron size to 89, 55.5, and 11.5 nm with reduction by 55, 90, and 434 times, respectively, due to ball milling for 30, 40, and 60 hours. The surfactant Triton X-100 was used to attain stability of 0.1% and 0.5% volume concentration of fly ash nanofluid. The samples were characterized by using scanning electron microscopy, dynamic light scattering, and zeta potential analysis. The outcome reveals that the thermal conductivity of fly ash nanofluid increases with temperature, volume concentration, and reduction in particle size. A maximum enhancement in thermal conductivity of 11.9% with 11.5-nm nanofluid sample and 5.4% with 89-nm nanofluid sample for 0.5% concentration at 60°C is observed. The viscosity of fly ash nanofluid increases with concentration and varies inversely with particle size and temperature. A difference of 1.6% in viscosity is observed between the values obtained with 11.5 and 89 nm nanofluid samples for 0.5% concentration at 30°C.