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51.
T. D. Dudderar C. P. Burger J. A. Gilbert J. A. Smith B. R. Peters 《Journal of Nondestructive Evaluation》1987,6(3):135-146
An innovative approach to nondestructive evaluation (NDE) using noncontacting optical sensors has demonstrated. In this effort a single mode optical fiber interferometer (OFI) was used to sense the presence and form of Rayleigh waves traveling along the surface of a steel test bar at a velocity of nearly 3mm/µs. Acousto-optic time-domain data was successfully used to detect the presence and locate the position of a test flaw (a machined slot) in the bar, and spectrum analysis was used to estimate its geometry and size. This approach has many potential applications in the ultrasonic evaluation of real flaws in structures with complex geometries. Coupled with the authors' earlier work demonstrating the feasibility of generating acoustic waves in metals using laser light pulses transmitted through the fiber optic probes, this latest achievement points to the development of a fully noncontacting, fiber optic based thermal-acousto-photonic (TAP) NDE system, with potential applications to the reliability testing of many important structures where composition, scale, geometry, or restricted access preclude the use of conventional NDE techniques. 相似文献
52.
53.
Gilbert Audira Petrus Siregar Kelvin H.-C. Chen Marri Jmelou M. Roldan Jong-Chin Huang Hong-Thih Lai Chung-Der Hsiao 《International journal of molecular sciences》2021,22(11)
Recently, medaka has been used as a model organism in various research fields. However, even though it possesses several advantages over zebrafish, fewer studies were done in medaka compared to zebrafish, especially with regard to its behavior. Thus, to provide more information regarding its behavior and to demonstrate the behavioral differences between several species of medaka, we compared the behavioral performance and biomarker expression in the brain between four medaka fishes, Oryzias latipes, Oryzias dancena, Oryzias woworae, and Oryzias sinensis. We found that each medaka species explicitly exhibited different behaviors to each other, which might be related to the different basal levels of several biomarkers. Furthermore, by phenomics and genomic-based clustering, the differences between these medaka fishes were further investigated. Here, the phenomic-based clustering was based on the behavior results, while the genomic-based clustering was based on the sequence of the nd2 gene. As we expected, both clusterings showed some resemblances to each other in terms of the interspecies relationship between medaka and zebrafish. However, this similarity was not displayed by both clusterings in the medaka interspecies comparisons. Therefore, these results suggest a re-interpretation of several prior studies in comparative biology. We hope that these results contribute to the growing database of medaka fish phenotypes and provide one of the foundations for future phenomics studies of medaka fish. 相似文献
54.
The formation of a series of semiinterpenetrating network (SIPN) hydrogels made by free‐radical copolymerization of N‐isopropylacrylamide (NIPA) and 2‐acrylamido‐2‐methylpropanesulphonic acid (AMPS) with varying comonomer mole ratios, crosslinked with N,N′‐methylene‐bisacrylamide (MBAA) in the presence of poly(ethylene glycol) (PEG) with average molecular weight 6,000 g mol?1 was studied via determination of complex viscosity, η*, using plate–plate rheometry. The isothermal time dependence of η* at various temperatures or the variation of η* with temperature of pregel solutions was utilized to detect the onset of gelation. The SIPN systems were compared with the corresponding gels made under the same conditions in the absence of PEG. The copolymer mainchain composition has a major effect on the time or temperature for onset of gelation and in particular gelation appears to be inhibited to some extent by MBAA when the AMPS/NIPA mole ratio in the pregel solution exceeds 0.5. The presence or absence of PEG in pregel solutions has a lesser effect on gelation. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 94: 2083–2087, 2004 相似文献
55.
Antoine T Priem B Heyraud A Greffe L Gilbert M Wakarchuk WW Lam JS Samain E 《Chembiochem : a European journal of chemical biology》2003,4(5):406-412
Two metabolically engineered Escherichia coli strains have been constructed to produce the carbohydrate moieties of gangliosides GM2 (GalNAcbeta-4(NeuAcalpha-3)Galbeta-4Glc; Gal = galactose, Glc = glucose, Ac = acetyl) and GM1 (Galbeta-3GalNAcbeta-4(NeuAcalpha-3)Galbeta-4Glc. The GM2 oligosaccharide-producing strain TA02 was devoid of both beta-galactosidase and sialic acid aldolase activities and overexpressed the genes for CMP-NeuAc synthase (CMP = cytidine monophosphate), alpha-2,3-sialyltransferase, UDP-GlcNAc (UDP = uridine diphosphate) C4 epimerase, and beta-1,4-GalNAc transferase. When this strain was cultivated on glycerol, exogenously added lactose and sialic acid were shown to be actively internalized into the cytoplasm and converted into GM2 oligosaccharide. The in vivo synthesis of GM1 oligosaccharide was achieved by taking a similar approach but using strain TA05, which additionally overexpressed the gene for beta-1,3-galactosyltransferase. In high-cell-density cultures, the production yields for the GM2 and GM1 oligosaccharides were 1.25 g L(-1) and 0.89 g L(-1), respectively. 相似文献
56.
Cameron Allan Gunn Jennifer L. DicksonChristopher G. Pretty Jane M. AlsweilerAdrienne Lynn Geoffrey M. ShawJ. Geoffrey Chase 《Computer methods and programs in biomedicine》2014
Introduction
Hyperglycaemia is a common complication of stress and prematurity in extremely low-birth-weight infants. Model-based insulin therapy protocols have the ability to safely improve glycaemic control for this group. Estimating non-insulin-mediated brain glucose uptake by the central nervous system in these models is typically done using population-based body weight models, which may not be ideal.Method
A head circumference-based model that separately treats small-for-gestational-age (SGA) and appropriate-for-gestational-age (AGA) infants is compared to a body weight model in a retrospective analysis of 48 patients with a median birth weight of 750 g and median gestational age of 25 weeks. Estimated brain mass, model-based insulin sensitivity (SI) profiles, and projected glycaemic control outcomes are investigated. SGA infants (5) are also analyzed as a separate cohort.Results
Across the entire cohort, estimated brain mass deviated by a median 10% between models, with a per-patient median difference in SI of 3.5%. For the SGA group, brain mass deviation was 42%, and per-patient SI deviation 13.7%. In virtual trials, 87–93% of recommended insulin rates were equal or slightly reduced (Δ < 0.16 mU/h) under the head circumference method, while glycaemic control outcomes showed little change.Conclusion
The results suggest that body weight methods are not as accurate as head circumference methods. Head circumference-based estimates may offer improved modelling accuracy and a small reduction in insulin administration, particularly for SGA infants. 相似文献57.
Johannes G. A. Terlingen Gijsbert A. J. Takens Frederik J. Van Der Gaag Allan S. Hoffman Jan Feijen 《应用聚合物科学杂志》1994,52(1):39-53
Poly(acrylic acid) (PAAc) films were treated with either an argon or a tetrafluoromethane (CF4) plasma and subsequently analyzed with X-ray photoelectron spectroscopy (XPS). PAAc films were decarboxylated during both types of plasma treatments. In addition, during the CF4 plasma treatment, the PAAc films became fluorinated. The plasma phase during the argon plasma treatment of PAAc films was investigated with optical emission spectroscopy. It was shown that during this plasma treatment carbon dioxide, water, and possibly hydrogen were liberated from the PAAc surface. By covering the surface of PAAc films with different materials (lithium fluoride, UV fused silica, and glass) during the plasma treatment, it was possible to differentiate between photochemically induced and particle-induced changes of the surface. This method was used to show that decarboxylation during the argon plasma treatment was caused by vacuum UV radiation (wavelength < 150 nm) and the decarboxylation/fluorination during the CF4 plasma treatment was induced by reactive fluorine-containing species from the plasma phase. Furthermore, during both processes, etching of the PAAc surface occurred. Based on these mechanisms, kinetic models were derived that could be used to describe the measured kinetic data adequately. © 1994 John Wiley & Sons, Inc. 相似文献
58.
The hexane-soluble fractions of hydroliquefied products from three Yallourn brown coal lithotypes have been separated into five fractions by combined silica-alumina packed column chromatography. Analyses of various fractions by g.c.-m.s. permitted the identification of ≈50 components in the saturate fraction and 40 components in the diaromatic fraction, together with 30 components in the monoaromatics. The components identified were quite similar among hexane-soluble portions of all three lithotypes. A marked predominance of even carbon number alkane (C23-C29) was observed in the hydrocarbon fractions from pale lithotype over , and medium light lithotype over both pure ZnCl2 and . However, medium dark lithotype over both melt catalysts produced a saturate fraction with an odd carbon number(C22-C28) preference. Based on spectral methods, Soxhlet extracts obtained from untreated lithotypes (hexane and benzene solubles) were characterized as complex mixtures of higher molecular weight(300–1000) aliphatic hydrocarbons, which were supposed to be a precursor of the saturates produced from the corresponding lithotype in the catalytic hydroliquefaction. 相似文献
59.
Nielsen Per F.; Roepstorff Peter; Clausen Ib G.; Jensen Ejner B.; Jonassen Ib; Svendsen Allan; Balschmidt Per; Hansen Finn B. 《Protein engineering, design & selection : PEDS》1989,2(6):449-457
Californium-252 plasma desorption mass spectrometry (PDMS) hasbeen employed for the characterization of a series of humaninsulin derivatives in order to evaluate the performance ofthis technique as an analytical tool in protein engineering.Several of the characterized modifications result in a 1 a.m.u.mass change. The precision in mass determination obtainableby PDMS analysis is not sufficient for unambiguous verificationof such modifications based on the molecular weight alone. Itis, however, possible to carry out in situ enzymatic digestionof the sample. Subsequent PDMS analysis will in most cases revealif the modification has been introduced as intended. 相似文献
60.
Alper Sarıoğlan Ömer Tunç Savaşçı Ayşe Erdem-Şenatalar Vu Thu Ha Gilbert Sapaly Younès Ben Taârit 《Catalysis Letters》2007,118(1-2):123-128
MFI type inorganic carrier was used in two different cationic forms, hydrogen and calcium respectively. MFI-supported molybdenum
and rhenium catalysts were prepared. The activities of the catalysts were compared for the aromatization reaction of methane.
Higher activity values were attained with the catalysts supported on HZSM-5. Aromatics were also observed with the catalysts
supported on CaZSM-5, despite their deficiency in acid sites. Highly dispersed rhenium is expected to be formed with the use
of the inorganic carrier in calcium form. On the other hand, lower reaction rates were observed with rhenium supported on
CaZSM-5, in spite of the improved dispersion of the active rhenium species on this catalyst. This was interpreted in terms
of the critical role of the acid sites in the conversion of methane to aromatics, compared to the improved dispersion of the
active metal. 相似文献