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排序方式: 共有226条查询结果,搜索用时 8 毫秒
221.
Andrade JM Aboy AL Apel MA Raseira MC Pereira JF Henriques AT 《Journal of food science》2011,76(8):C1181-C1187
Fruits of Myrcianthes pungens Berg. Legr. (Myrtaceae), known as guabiju, are widely consumed fresh as well as dried, processed into jam, marmalade, and juices. In this study, chemical composition and antichemotactic and antioxidant activities of fruits from a wild type (GB) and 2 genotypes, PL2 and PL1, of guabiju were investigated. Total anthocyanins for the genotypes ranged from 334 to 531 mg/100 g dry weight (dwt). Total flavonoids and polyphenols ranged from 79.8 to 154 mg/100 g and 2438 to 4613 mg/100 g (dwt), respectively. A reversed phase liquid chromatography method with photodiode array detection was used to determine chemical profiles of the main anthocyanins found in the extracts. An HPLC method for the quantification of flavonoids is proposed, providing a simple procedure with rapid sample preparation. All samples contained 5 identical anthocyanidins, distributed differently, with cyanidin as the main compound. Identified flavonoids were quercitrin, hyperoside, and isoquercitrin; their relative amounts varied among the extracts. The antioxidant activity of guabiju methanolic extract was comparable to that of Trolox, and at a test concentration of 0.25 mg/mL, GB and PL2 activities were higher than those exhibited by Trolox. Total dry extracts of guabiju exhibited greater inhibition of chemotaxis at a concentration of 4 μg/mL, except for GBH (wild-type hydrolyzed extract) which already presented high values at a concentration of 2 μg/mL. These results suggest that the consumption of this fruit, rich in polyphenols, may be beneficial to human health. Practical Application: The paper is the first attempt on the improvement of this native fruit, since it is widely consumed regularly as part of the South American diet. The content of phenolic compounds demonstrates that consumption of guabiju would be beneficial to human health. Differences among samples, originating from open pollination of plants growing on the same site, lead to the conclusion that improvements can be made in the chemical composition and beneficial activity of guabiju fruits by simply selecting genotypes for these characteristics among open-pollinated seedlings. 相似文献
222.
Corradini MG Amézquita A Normand MD Peleg M 《International journal of food microbiology》2006,106(2):223-228
Published experimental isothermal growth curves of Clostridium perfringens cells in ground ham were fitted with a modified three-parameter version of the logistic equation as a primary model and the temperature dependence of the three parameters by ad hoc empirical secondary models. These were used to predict the organism's non-isothermal growth curves under three different cooling regimes. The assumption has been that the organism's instantaneous (or momentary) non-isothermal growth rate is the isothermal rate at the given temperature at a time that corresponds to its instantaneous population size. This could be translated into a differential rate model equation, whose coefficients are constructed from terms that reflect the changing growth parameters with temperature and hence with time. The continuous rate equation, however, can be solved incrementally by a numerical procedure that can be implemented in similar purpose software like Microsoft Excel(R). In all three cases, there was good agreement between the growth curves predicted by the model and those found experimentally. This demonstrated that the procedure can be used to generate growth curves under complicated thermal histories that may include regular and irregular temperature oscillations. 相似文献
223.
Seungnyeong Heo Jonghwi Baek Juhyun Bae Prof. Dr. Bo Am Seo Seongjun Kim Subin Jeong Suhyun Kim Dr. Yiseul Ryu Prof. Dr. Joong-jae Lee 《Chembiochem : a European journal of chemical biology》2023,24(11):e202200700
Horseradish peroxidase (HRP) is a pivotal biocatalyst for biosensor development and fine chemical synthesis. HRP proteins are mostly extracted and purified from the roots of horseradish because the solubility and productivity of recombinant HRP in bacteria are significantly low. In this study, we investigate the reconstitution system of split HRP fragments to improve its soluble expression levels in E. coli allowing the cost-effective production of bioactive HRPs. To promote the effective association between two HRP fragments (HRPn and HRPc), we exploit SpyTag-SpyCatcher chemistry, a versatile protein coupling method with high affinity and selectivity. Each HRP fragment was genetically fused with SpyTag and SpyCatcher, respectively, exhibiting soluble expression in the E. coli cytoplasm. The engineered split HRPs were effectively and irreversibly reconstituted into a biologically active and stable assembly that can catalyze intrinsic enzymatic reactions. Compared to the chaperone co-expression system, our approach shows that the production yield of soluble HRP is comparable, but the purity of the final product is relatively high. Therefore, our results can be applied to the high-yield production of recombinant HRP variants and other difficult-to-express proteins in bacteria without complex downstream processes. 相似文献
224.
Víctor García-López Niccolò Giaconi Lorenzo Poggini Joaquín Calbo Amélie Juhin Brunetto Cortigiani Javier Herrero-Martín Enrique Ortí Matteo Mannini Miguel Clemente-León Eugenio Coronado 《Advanced functional materials》2023,33(44):2300351
The synthesis and characterization of a new Co(II) spin-crossover (SCO) complex based on 4′-(4-carboxyphenyl)−2,2′:6′,2″-terpyridine ligand are reported. This complex can be successfully grafted on silver surface maintaining the SCO behavior. Thus, atomic force microscopy (AFM), matrix assisted laser desorption ionization – time-of-flight mass spectrometry (MALDI-TOF MS), Raman spectroscopy, and XPS measurements, upon surface deposition, evidence the formation of a monolayer of intact molecules grafted through carboxylate groups to the Ag surface. Three different techniques: Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), and X-ray absorption spectroscopy (XAS), supported by first-principles calculations, confirm that the deposited molecules undergo a gradual spin transition with temperature. This phenomenon is unprecedented for a monolayer of molecules directly grafted onto a metallic surface from solution. 相似文献
225.
226.
Dr. Mohamed Ettaoussi Amélie Laversin Brandon Vreulz Dr. Marouane Rami Dr. Nicolas Lebegue Dr. Philippe Delagrange Dr. Daniel Henri Caignard Prof. Patricia Melnyk Dr. Maxime Liberelle Dr. Saïd Yous 《ChemMedChem》2022,17(3):e202100658
In our constant search for new successors of agomelatine, we report herein a new series of compounds resulting from bioisosteric modulation of the naphthalene ring. The isoquinoline and tetrahydroisoquinoline derivatives were synthesized and pharmacologically evaluated. This isosteric replacement of the naphthalene group of agomelatine has led to potent agonist and partial agonist compounds with nanomolar melatonergic binding affinities. Overall, the presence of a nitrogen atom was accompanied with a decrease in the binding affinity toward both MT1 and MT2 and the loss of 5HT2C response, especially for tetrahydroisoquinoline in comparison with the parent compound. Interestingly, due to the presence of this nitrogen atom, a notable improvement in the pharmacokinetic properties was observed for all compounds. 相似文献