Eosinophils and serum eosinophilic cationic proteins in interleukin-2-based immunotherapy for cancer

BACKGROUND: Extramedullary hematopoiesis (EMH) occurs in many disorders, including thalassemias and other hemoglobinopathies, and commonly presents in the spleen and liver. We present a case of spinal cord compression in a patient with beta-thalassemia intermedia, and review the literature and available treatment options. PATIENT AND METHODS: A 35-year-old black female with beta-thalassemia intermedia presented with a 3-week history of back pain and lower extremity weakness. Neurologic examination was consistent with spinal cord compression, and gadolinium enhanced magnetic resonance imaging (MRI) confirmed this diagnosis. She was given intravenous steroids and radiotherapy was begun in 200 cGy fractions to a total dose of 2000 cGy. RESULTS: At the completion of radiotherapy the patient was ambulatory with mild residual weakness. MRI scans 16 months later showed smaller, but persistent masses, and she remains asymptomatic 5 years from her diagnosis. CONCLUSION: Recognition of spinal cord EMH requires prompt physical examination and MRI for accurate diagnosis. EMH can be managed with radiation, surgery, transfusions, or a combination of these therapies. Radiation in conservative doses of (750-3500 cGy) is non-invasive, avoids the surgical risks of potentially severe hemorrhage and incomplete resection, and has a high complete remission rate in the majority of patients. Relapse rates are moderate (37.5%), but retreatment provides excellent chance for second remission.     

Genetic variants of human serum albumin in Italy: point mutants and a carboxyl-terminal variant

Of the > 50 different genetic variants of human serum albumin (alloalbumins) that have been characterized by amino acid or DNA sequence analysis, almost half have been identified in Italy through a long-term electrophoretic survey of serum. Previously we have reported structural studies of 11 Italian alloalbumins with point mutations, 2 different carboxyl-terminal variants, and 1 case of analbuminemia in an Italian family. This article describes confirmation by DNA sequencing of mutations previously inferred from protein sequencing of 4 of the above alloalbumins; it also reports the mutations identified by protein and DNA sequence analysis of 4 other Italian alloalbumins not previously recorded: albumin Larino, His3-->Tyr; Tradate-2 (protein sequencing only), Lys225-->Gln; Caserta, Lys276-->Asn; and Bazzano, a carboxyl-terminal variant. The first 3 have point mutations that produce a single amino acid substitution, but a nucleotide deletion causes a frameshift and an altered and truncated carboxyl-terminal sequence in albumin Bazzano. In these 4 instances the expression of the alloalbumin is variable, ranging from 10% to 70% of the total albumiN, in contrast to the usual 50% each for the normal and mutant albumin. The distribution of point mutations in the albumin gene is nonrandom; most of the 47 reported point substitutions involve charged amino acid residues on the surface of the molecule that are not concerned with ligand-binding sites.     

Natural compounds to preserve fresh fish burgers

Natural antimicrobial compounds to control the quality decay of a fresh fish burger were studied. In particular, thymol, lemon extract and grape fruit seed extract (GFSE), at 20, 40 and 80 ppm, have been tested against the main spoilage microorganisms inoculated in fish burgers stored at 5 °C. The evolution of mesophilic and psychrotrophic bacteria was also monitored. The sensorial quality decay was determined by means of a panel test, which assessed odour, colour, texture, drip loss and general appearance during the storage period. Results show that all the active substances efficiently slowed down the growth of the spoilage microorganisms. In particular, GFSE was the most efficient against Photobacterium phosphoreum , Shewanella putrefaciens and mesophilic bacteria, whereas thymol was the most efficient against both psychrotrophic bacteria and Pseudomonas fluorescens . Microbial growth was the factor limiting fresh fish burger acceptability.     

Beam dumps design and local radiation protection at TERA synchrotron

The realisation of the National Center of Hadrontherapy was funded by the Italian Government in 2002. The Centre will be built in the area of Pavia (Italy). The synchrotron designed in the framework of this programme will accelerate protons and carbon ions up to 250 MeV and 400 MeV u(-1), respectively. Some of the main aspects which were taken into account in the design of the acceleration system are the patient's safety and the beam control. From this point of view an important role is played by the beam dumps in the synchrotron ring and upstream of the extraction system. In particular, an horizontal and a vertical beam dump will be installed in the synchrotron ring: the former will be used for lowering the beam intensity and the latter for beam abortion. The dump at the extraction will absorb the particles during the mounting and the falling ramps of the synchrotron magnetic cycle, thus extracting only the flat top of the ion spill. Beam dumps can produce intense fields of secondary radiation (neutrons, charged light-hadrons and photons) and high rates of induced activity, since they can absorb the beam completely. Usually they have to be shielded to protect the electronics during machine operation and to attenuate the radiation dose below the limits imposed by the law when the personnel access to the synchrotron hall. The part of the shielding design of the beam dumps concerning with the acceleration of protons was made using Monte Carlo simulations with the FLUKA code. Both induced activity and secondary radiation were taken into account. The shields against secondary radiation produced by carbon ions were designed, referring only to secondary neutrons, taking double-differential distributions from the literature as sources for the FLUKA simulations. The induced activity from carbon ions interactions was estimated analytically, using the data generated by the EPAX 2 code. The dose-equivalent rates from the induced radionuclides were calculated at 1 m from the shielded dumps, taking into account the contribution of activated components of the synchrotron ring.     

Shelf life of ready to use peeled shrimps as affected by thymol essential oil and modified atmosphere packaging

In this work the influence of different packaging strategies on the shelf life of ready to use peeled shrimps was investigated. First, the effectiveness of the coating (Coat) and the active coating loaded with different concentrations of thymol (Coat-500, Coat-1000, and Coat-1500) on the quality loss of the investigated food product packaged in air was addressed; afterwards, the thymol concentration that had shown the best performance was used in combination with MAP (5% O₂; 95% CO₂). Microbial cell load of main spoilage microorganisms, pH and sensorial quality were monitored during the refrigerated storage. Results of the first step suggested that the sole coating did not affect the microbial growth. A slight antimicrobial effect was obtained when the coating was loaded with thymol and a concentration dependence was also observed. Moreover, the active coating was effective in minimizing the sensory quality loss of the investigated product, it was particularly true at the lowest thymol concentration. In the second step, the thymol concentration (1000 ppm) that showed the strike balance between microbial and sensorial quality was chosen in combination with MAP. As expected, MAP significantly affected the growth of the mesophilic bacteria. In particular, a cell load reduction of about 2 log cycle for the samples under MAP respect to that in air was obtained. Moreover, the MAP packaging inhibited the growth of the Pseudomonas spp. and hydrogen sulphide-producing bacteria. The MAP alone was not able to improve the shelf life of the uncoated samples. In fact, no significant difference between the control samples packaged in air and MAP was observed. Whilst, the use of coating under MAP condition prolonged the shelf life of about 6 days with respect to the same samples packaged in air. Moreover, when the MAP was used in combination with thymol, a further shelf life prolongation with respect to the samples packaged in air was observed. In particular, a shelf life of about 14 days for the active coating under MAP compared to the same samples in air (5 days) was obtained.     

A Mobility-Prediction-Based Relay Deployment Framework for Conserving Power in MANETs

There has been a growing interest in designing mobile systems consisting of special relay nodes whose mobility can be controlled by the underlying network. In this paper, we consider the design of a heterogeneous mobile ad hoc network (MANET) consisting of two kinds of mobile nodes-traditional nodes with limited energy and a few controllable mobile relay nodes with relatively abundant energy resources. We propose a novel relay deployment framework that utilizes mobility prediction and works in tandem with the underlying MANET routing protocol to optimally define the movement of the relay nodes. We present two instances of the relay deployment problem, together with the solutions, to achieve different goals. Instance 1, termed Min-Total, aims to minimize the total energy consumed across all the traditional nodes during data transmission, while instance 2, termed Min-Max, aims to minimize the maximum energy consumed by a traditional node during data transmission. Our solutions also enable the prioritization of individual nodes in the network based on residual energy profiles and contextual significance. We perform an extensive simulation study to understand the trade-offs involved in deploying an increasing fraction of such relay nodes in the network. We also investigate the performance of the proposed framework under different mobility prediction schemes. Results indicate that even when the relay nodes constitute a small fraction of the total nodes in the network, the proposed framework results in significant energy savings. Further, we observed that while both the schemes have their potential advantages, the differences between the two optimization schemes are clearly highlighted in a sparse network.