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101.
The gene encoding the human erythrocyte form of cytochrome b5 (97 residues in length) has been prepared by mutagenesis of an expression vector encoding lipase-solubilized bovine liver microsomal cytochrome b5 (93 residues in length) (Funk et al., 1990). Efficient expression of this gene in Escherichia coli has provided the first opportunity to obtain this protein in quantities sufficient for physical and functional characterization. Comparison of the erythrocytic cytochrome with the trypsin-solubilized bovine liver cytochrome b5 by potentiometric titration indicates that the principal electrostatic difference between the two proteins results from two additional His residues present in the human erythrocytic protein. The midpoint reduction potential of this protein determined by direct electrochemistry is -9 +/- 2 mV vs SHE at pH 7.0 (mu = 0.10 M, 25.0 degrees C), and this value varies with pH in a fashion that is consistent with the presence of a single ionizable group that changes pKa from 6.0 +/- 0.1 in the ferricytochrome to 6.3 +/- 0.1 in the ferrocytochrome with delta H degrees = -3.2 +/- 0.1 kcal/mol and delta S degrees = -11.5 +/- 0.3 eu (pH 7.0, mu = 0.10). The 1D 1H NMR spectrum of the erythrocytic ferricytochrome indicates that 90% of the protein binds heme in the "major" orientation and 10% of the protein binds heme in the "minor" orientation (pH 7.0, 25 degrees C) with delta H degrees = -2.9 +/- 0.3 kcal/mol and delta S degrees = -5.4 +/- 0.9 eu for this equilibrium. 相似文献
102.
In today's highly interconnected, uncertain and dynamic business environment, team boundary spanning has become an important determinant of the performance of new product development (NPD) projects. Despite the positive evidence supporting the use of boundary spanning activities by NPD teams, little is still known about how boundary spanning teams become high-performance teams. The current study advances research on this subject by examining the mediating effect of team potency on the relationship between team boundary spanning and new product performance, as well as the moderating effects of team size and functional diversity on the relationship between team boundary spanning and team potency. Data from a time-lagged survey study of 140 NPD projects found that team boundary spanning can promote team potency that, in turn, results in greater new product quality and new product creativity. The positive effect of team boundary spanning on team potency was found to be more pronounced for NPD teams of medium size and high levels of functional diversity. 相似文献
103.
Fouto Ana R. Nunes Rita G. Pinto Joana Alves Luísa Calado Sofia Gonçalves Carina Rebolo Margarida Viana-Baptista Miguel Vilela Pedro Figueiredo Patrícia 《Magma (New York, N.Y.)》2022,35(5):779-790
Magnetic Resonance Materials in Physics, Biology and Medicine - Histogram-based metrics extracted from diffusion-tensor imaging (DTI) have been suggested as potential biomarkers for cerebral small... 相似文献
104.
Paiva Pedro Yuri Arbs Moreno Camila Castro Smith-Miles Kate Valeriano Maria Gabriela Lorena Ana Carolina 《Machine Learning》2022,111(8):3085-3123
Machine Learning - Machine Learning studies often involve a series of computational experiments in which the predictive performance of multiple models are compared across one or more datasets. The... 相似文献
105.
106.
Cyril Dedieu Anaïs Barasinski Francisco Chinesta Jean-Marc Dupillier 《International Journal of Material Forming》2017,10(5):779-792
In a context of cost reduction, in situ filament winding of thermoplastic matrix composites becomes an appealing process. As residual stresses could considerably affect the produced part, models were proposed to predict process-induced residual stresses. After developing a validated thermal model of the process, mainly three different aspects are here addressed: the continuous bonding occurring during the process, the effect of the processed layer on the structure, and the effect of the curvature of the mandrel. While stresses coming from the continuous bonding appeared to be negligible, consequent levels of stresses can be achieved due to an iterative compression of the structure by the tow (supposed to be under tension). The mandrel properties and the tow tension profile followed during winding are essential parameters that might induce several different stress states. A comparison between measured and computed end-to-end openings of split rings illustrates the accuracy of the proposed models. 相似文献
107.
108.
Sergio S��nchez Garc��a Ana G��mez Oliva Emilia P��rez Belleboni Iv��n Pau de la Cruz 《International Journal of Information Security》2011,10(6):351-372
At present, many countries allow citizens or entities to interact with the government outside the telematic environment through
a legal representative who is granted powers of representation. However, if the interaction takes place through the Internet,
only primitive mechanisms of representation are available, and these are mainly based on non-dynamic offline processes that
do not enable quick and easy identity delegation. This paper proposes a system of dynamic delegation of identity between two
generic entities that can solve the problem of delegated access to the telematic services provided by public authorities.
The solution herein is based on the generation of a delegation token created from a proxy certificate that allows the delegating
entity to delegate identity to another on the basis of a subset of its attributes as delegator, while also establishing in
the delegation token itself restrictions on the services accessible to the delegated entity and the validity period of delegation.
Further, the paper presents the mechanisms needed to either revoke a delegation token or to check whether a delegation token
has been revoked. Implications for theory and practice and suggestions for future research are discussed. 相似文献
109.
In a preordered set, or preset, consequence operators in the sense of Tarski, defined on families of subsets, are introduced. From them, the corresponding sets of conjectures, hypotheses, speculations and refutations are considered, studying the relationships between these sets and those previously defined on ortholattices. All the concepts introduced are illustrated with three particular consequence operators, whose behavior is studied in detail. The results obtained are applied to the case of fuzzy sets endowed with the usual pointwise ordering. 相似文献
110.
M. Ferrer D. Karatzas E. Valveny I. Bardaji H. Bunke 《Computer Vision and Image Understanding》2011,115(7):919-928
The median graph has been shown to be a good choice to obtain a representative of a set of graphs. However, its computation is a complex problem. Recently, graph embedding into vector spaces has been proposed to obtain approximations of the median graph. The problem with such an approach is how to go from a point in the vector space back to a graph in the graph space. The main contribution of this paper is the generalization of this previous method, proposing a generic recursive procedure that permits to recover the graph corresponding to a point in the vector space, introducing only the amount of approximation inherent to the use of graph matching algorithms. In order to evaluate the proposed method, we compare it with the set median and with the other state-of-the-art embedding-based methods for the median graph computation. The experiments are carried out using four different databases (one semi-artificial and three containing real-world data). Results show that with the proposed approach we can obtain better medians, in terms of the sum of distances to the training graphs, than with the previous existing methods. 相似文献