Gel mechanical properties of milk whey protein–dextran conjugates obtained by Maillard reaction

The effect of Maillard reaction on the mechanical properties of whey protein isolate (WPI) heat-induced gels was evaluated. WPI and dextran (15–25 kDa) conjugates were obtained by controlled dry heating during storage at 60 °C and 63% relative humidity for 2, 5 and 9 days. Changes in browning intensity and content of free amino groups were used to estimate the Maillard reaction. A decrease in free amino groups of WPI was observed when increasing polysaccharide concentration and reaction time. An increase in both a* and b* CIE Lab colour parameters indicated the development of a reddish-brown colour, typical of the Maillard reaction. Uniaxial compression and stress relaxation tests were performed to measure the mechanical properties of mixed and conjugate gels. Maillard reaction significantly modified the mechanical properties of WPI/DX gels, and even prevented fracture when conjugate gels were subjected to 80% deformation in uniaxial compression test.     

Microfluidic-Generated Seeds for Gold Nanotriangle Synthesis in Three or Two Steps

Nanoparticle synthesis has drawn great attention in the last decades. The study of crystal growth mechanisms and optimization of the existing methods lead to the increasing accessibility of nanomaterials, such as gold nanotriangles which have great potential in the fields of plasmonics and catalysis. To form such structures, a careful balance of reaction parameters has to be maintained. Herein, a novel synthesis of gold nanotriangles from seeds derived with a micromixer, which provides a highly efficient mixing and simple parameter control is reported. The impact of the implemented reactor on the primary seed characteristics is investigated. The following growth steps are studied to reveal the phenomena affecting the shape yield. The use of microfluidic seeds led to the formation of well-defined triangles with a narrower size distribution compared to the entirely conventional batch synthesis. A shortened two-step procedure for the formation of triangles directly from primary seeds, granting an express but robust synthesis is further described. Moreover, the need for a thorough study of seed crystallinity depending on the synthesis conditions, which – together with additional parameter optimization – will bring a new perspective to the use of micromixers which are promising for scaling up nanomaterial production is highlighted.     

A glucose-specific metric to assess predictors and identify models

In diabetes, the mean square error (MSE) metric is extensively used for assessing glucose prediction methods and identifying glucose models. One limitation of this metric is that, by equally treating errors in hypo-, eu-, and hyperglycemia, it is not able to weight the different clinical impact of errors in these three situations. In this paper, we propose a new cost function, which overcomes this limitation and can be used in place of MSE for several scopes, in particular for assessing the quality of glucose predictors and identifying glucose models. The new metric called glucose-specific MSE (gMSE) modifies MSE with a Clark error grid inspired penalty function, which penalizes overestimation in hypoglycemia and underestimation in hyperglycemia, i.e., the most harmful conditions on a clinical perspective. From a mathematical point of view, gMSE retains sensitivity of MSE and inherits some of its important mathematical features, in particular it has no local minima, simplifying the optimization. This makes it suitable for model identification purposes also. First, the goodness of it is demonstrated by means of three experiments, designed ad hoc to evidence its sensitivity to accuracy, precision, and distortion in glucose predictions. Second, a prediction assessment problem is presented, in which two real prediction profiles are compared. Results show that the MSE chooses the worst clinical situation, while gMSE correctly selects the situation with less clinical risk. Finally, we also demonstrate that models identified minimizing gMSE are more accurate in potentially harmful situations (hypo- and hyperglycemia) than those obtained by MSE.     

Critical water activity for the preservation of Lactobacillus bulgaricus by vacuum drying

Lactobacillus delbrueckii subsp. bulgaricus was dried under vacuum at different temperatures and its preservation evaluated analyzing the evolution of three parameters throughout the process: lag time, percentage of membrane damage and zeta potential. Microorganisms were dehydrated at 30, 45 and 70 degrees C in a vacuum centrifuge for different times. The aw achieved for each time of drying was correlated with the cell recovery at all the temperatures assayed. The recovery of microorganisms was evaluated by means of: a) kinetics of growth in milk after drying, as a measure of the global damage; b) quantification of the membrane damage using the fluorescent dyes SYTO 9 and PI; c) determination of changes in the superficial charges (zeta potential) as measured of the increase in the hydrophobic residues exposed in the bacterial surface after dehydration. These changes correlate well with the bacterial damage occurred during the dehydration process. The Page's equation allowed fitting of aw and time of drying, thus making possible the determination of the appropriate dehydration conditions (time-temperature ratios) for which no cell damage occurs. The evaluation of three parameters (lag time, percentage of membrane damage and zeta potential) allowed us to conclude that at the lowest temperature of dehydration, the first target of damage is the cell membrane. However, this damage is not decisive for the bacterial recovery after rehydration, as are the increase in the lag time and the changes in the zeta potential, as was observed for L. bulgaricus dehydrated at 45 and 70 degrees C for larger times.     

Task Switching After Cerebellar Damage.

The authors of this study investigated task switching following cerebellar damage. The study group consisted of 7 children and adolescents (M age = 13.8 years) who underwent surgical removal of a benign posterior fossa tumor. They were tested at a sufficient interval after surgery (M lag = 6.13 years) for restoration of normal cognitive skills and intelligence. Although all showed normal learning of the task compared with control participants, when rapid behavioral changes were required (short preparation time), they exhibited behavioral rigidity manifested by enhanced switching cost. These results are in line with another study on serial reaction time with the same patients (A. Berger et al., in press). They have important implications for our understanding of the cognitive sequelae of early cerebellar damage as well as the involvement of the cerebellum in task switching. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Fines Accumulation and Distribution in a Storm-Water Rain Garden Nine Years Postconstruction

Storm water control measures (SCMs), also known as best management practices (BMPs), such as rain gardens, are designed to infiltrate storm-water runoff and reduce pollutant transport to surface waters. The life span of these SCMs may be limited depending on the composition of sediments in runoff water. Settling of fine sediments may clog soil pore spaces, reducing the infiltration capacity of the soil and reducing the potential benefits of this SCM. A study was conducted on a Villanova campus rain garden that accepts runoff from an adjacent parking lot to determine if there was a relationship between the accumulation of fine sediments over time and the infiltration capacity. The soil textural profile within the rain garden was characterized prior to SCM installation (2001), after installation, after five years, and after seven years of receiving storm-water runoff. Infiltration data were collected by the single-ring infiltrometer method in 2006 and 2009. Differences in soil texture were found between locations within the infiltration basin, and accumulation of fines smaller than 0.1 mm was observed at both locations sampled in 2009. Infiltration rates were significantly different between the two locations measured within the rain garden, but infiltration rates did not change significantly over time within those regions. This SCM was designed at a 10:1 watershed to SCM area ratio, which is twice what is recommended by the PA DEP BMP Manual. The data collected over the seven years since installation indicate that while fines have accumulated in the SCM there has been no significant change in infiltration potential.     

Genomic Analysis Made It Possible to Identify Gene-Driver Alterations Covering the Time Window between Diagnosis of Neuroblastoma 4S and the Progression to Stage 4

Neuroblastoma (NB) is a tumor of the developing sympathetic nervous system. Despite recent advances in understanding the complexity of NB, the mechanisms that determine its regression or progression are still largely unknown. Stage 4S NB is characterized by a favorable course of disease and often by spontaneous regression, while progression to true stage 4 is a very rare event. Here, we focused on genomic analysis of an NB case that progressed from stage 4S to stage 4 with a very poor outcome. Array-comparative genomic hybridization (a-CGH) on tumor-tissue DNA, and whole-exome sequencing (WES) on exosomes DNA derived from plasma collected at the onset and at the tumor progression, pointed out relevant genetic changes that can explain this clinical worsening. The combination of a-CGH and WES data allowed for the identification iof somatic copy number aberrations and single-nucleotide variants in genes known to be responsible for aggressive NB. KLRB1, MAPK3 and FANCA genes, which were lost at the time of progression, were studied for their possible role in this event by analyzing in silico the impact of their expression on the outcome of 786 NB patients.     

Malignant Transformation of Giant Cell Tumour of Bone: A Review of Literature and the Experience of a Referral Centre

Giant cell tumour of bone (GCTB) is a benign, locally aggressive primary bone neoplasm that represents 5% of all bone tumours. The principal treatment approach is surgery. Although generally GCTB is considered only a locally aggressive disease, it can metastasise, and lung metastases occur in 1–9% of patients. To date, only the use of denosumab has been approved as medical treatment for GCTB. Even more rarely, GCTB undergoes sarcomatous transformation into a malignant tumour (4% of all GCTB), but history of this malignant transformation is unclear and unpredictable. Considering the rarity of the event, the data in the literature are few. In this review, we summarise published data of GCTB malignant transformation and we analyse three cases of malignant transformation of GCTB, evaluating histopathology, genetics, and radiological aspects. Despite the rarity of this event, we conclude that a strict follow up is recommended to detect early malignant transformation.