Extracellular recordings from patterned neuronal networks using planar microelectrode arrays

Neuronal cell networks have been reconstructed on planar microelectrode arrays (MEAs) from dissociated hippocampal pyramidal neurons. Microcontact printing (microCP) and a photoresist-liftoff method were used to selectively localize poly-L-lysine (PLL) on the surface of MEAs. Haptotaxis led to the organization of the neurons into networks localized adjacent to microelectrodes. Various grids of PLL with 2-25-microm-wide lines spaced by 50-200 microm with 15-25-microm nodes at intersection points were used to guide cell body attachment and neurite outgrowth. Bursting activity with spike amplitude attenuation was observed, and multichannel recordings detected instances of coincident firing activity. Finally, we present here an extracellular recording from a approximately 2 microm bundle of guided neurites.     

Myrosinase activity of cruciferous vegetables

Myrosinase activity in partially purified extracts of 12 cruciferous vegetables and an acetone powder preparation of Sinapis alba L. (white mustard) was determined by the initial rate of glucose formation from glucosinolate hydrolysis using a coupled assay. Of the species studied Raphanus sativus L. (radish, 12.8±0.7 μmol min^?1g^?1 powdered tissue) had the greatest myrosinase activity, and Brassica campestris L. ssp. rapifera (turnip) and Nasturtium officinalis R.Br. (watercress) (0.6±0.1 and 0.8±0.03 μmol min^?1g^?1 powdered tissue respectively) the least. The sub-species of Brassica oleracea studied all had similar myrosinase activity (ca 2.5±0.2μmolmin^?1g^?1 powdered tissue) except B. oleracea L. var. gemmifera D.C. (Brussels sprouts) and B. oleracea L. var. capitata L. (white cabbage) which had higher activities (7.6±0.1 and 5.2±0.2μmol min^?1g^?1 powdered tissue respectively). The effect of ascorbate concentration upon the myrosinase activity of six of the crucifers studied and the white mustard preparation, revealed that the ascorbate concentration necessary to promote maximal activity varied with species. A concentration of 0.9mM ascorbate maximally activated radish and turnip myrosinase, while red cabbage, watercress, white mustard and Brussels sprouts were maximally activated at 2.0, 3.0, 5.0 and 0.7–1.0mM ascorbate respectively. Two peaks of maximal myrosinase activity, occurring between 0.9 and 1.0mM and at 3.0mM ascorbate, were found for B. oleracea L. var. botrytis L. subvar. cauliflora D.C. (cauliflower).     

Photoclickable Surfaces for Profluorescent Covalent Polymer Coatings

The nitrile imine‐mediated tetrazole‐ene cycloaddition reaction (NITEC) is introduced as a powerful and versatile conjugation tool to covalently ligate macromolecules onto variable (bio)surfaces. The NITEC approach is initiated by UV irradiation and proceeds rapidly at ambient temperature yielding a highly fluorescent linkage. Initially, the formation of block copolymers by the NITEC methodology is studied to evidence its efficacy as a macromolecular conjugation tool. The grafting of polymers onto inorganic (silicon) and bioorganic (cellulose) surfaces is subsequently carried out employing the optimized reaction conditions obtained from the macromolecular ligation experiments and evidenced by surface characterization techniques, including X‐ray photoelectron spectroscopy and FT‐IR microscopy. In addition, the patterned immobilization of variable polymer chains onto profluorescent cellulose is achieved through a simple masking process during the irradiation.     

Tuneable Singlet Exciton Fission and Triplet–Triplet Annihilation in an Orthogonal Pentacene Dimer

Fast and highly efficient intramolecular singlet exciton fission in a pentacene dimer, consisting of two covalently attached, nearly orthogonal pentacene units is reported. Fission to triplet excitons from this ground state geometry occurs within 1 ps in isolated molecules in solution and dispersed solid matrices. The process exhibits a sensitivity to environmental polarity and competes with geometric relaxation in the singlet state, while subsequent triplet decay is strongly dependent on conformational freedom. The near orthogonal arrangement of the pentacene units is unlike any structure currently proposed for efficient singlet exciton fission and may lead to new molecular design rules.     

Neuromuscular Interfacing: Establishing an EMG-Driven Model for the Human Elbow Joint

Assistive devices aim to mitigate the effects of physical disability by aiding users to move their limbs or by rehabilitating through therapy. These devices are commonly embodied by robotic or exoskeletal systems that are still in development and use the electromyographic (EMG) signal to determine user intent. Not much focus has been placed on developing a neuromuscular interface (NI) that solely relies on the EMG signal, and does not require modifications to the end user's state to enhance the signal (such as adding weights). This paper presents the development of a flexible, physiological model for the elbow joint that is leading toward the implementation of an NI, which predicts joint motion from EMG signals for both able-bodied and less-abled users. The approach uses musculotendon models to determine muscle contraction forces, a proposed musculoskeletal model to determine total joint torque, and a kinematic model to determine joint rotational kinematics. After a sensitivity analysis and tuning using genetic algorithms, subject trials yielded an average root-mean-square error of 6.53° and 22.4° for a single cycle and random cycles of movement of the elbow joint, respectively. This helps us to validate the elbow model and paves the way toward the development of an NI.     

Novel,Robust, and Versatile Bijels of Nitromethane,Ethanediol, and Colloidal Silica: Capsules,Sub‐Ten‐Micrometer Domains,and Mechanical Properties

Bicontinuous, interfacially jammed emulsion gels (bijels) are a class of soft solid materials in which interpenetrating domains of two immiscible fluids are stabilized by an interfacial colloidal monolayer. Such structures form through the arrest of the spinodal decomposition of an initially single‐phase liquid mixture containing a colloidal suspension. With the use of hexalmethyldisilazane, the wetting character of silica colloids, ranging in size and dye content, can be modified for fabricating a novel bijel system comprising the binary liquid ethanediol–nitromethane. Unlike the preceding water‐lutidine based system, this bijel is stable at room temperature and its fabrication and resultant manipulation are comparatively straightforward. The new system has facilitated three advancements: firstly, we use sub 100 nm silica particles to stabilize the first bijel made from low molecular weight liquids that has domains smaller than ten micrometers. Secondly, our new and robust bijel permits qualitative rheological work which reveals the bijel to be significantly elastic and self healing whilst its domains are able to break, reform and locally rearrange. Thirdly, we encapsulate the ethanediol–nitromethane bijel in Pickering drops to form novel particle‐stabilized bicontinuous multiple emulsions that we christen bijel capsules. These emulsions are stimuli responsive – they liberate their contained materials in response to changes in temperature and solvency, and hence they show potential for controlled release applications.     

Role of hydrogen in the surface passivation of crystalline silicon by sputtered aluminum oxide

Aluminum oxide films can provide excellent surface passivation on both p‐type and n‐type surfaces of silicon wafers and solar cells. Even though radio frequency magnetron sputtering is capable of depositing aluminum oxide with concentrations of negative charges comparable to some of the other deposition methods, the surface passivation has not been as good. In this paper, we compare the composition and bonding of aluminum oxide deposited by thermal atomic layer deposition and sputtering, and find that the interfacial silicon oxide layer and hydrogen concentration can explain the differences in the surface passivation. Copyright © 2010 John Wiley & Sons, Ltd.     

An energy‐efficient and balanced clustering approach for improving throughput of wireless sensor networks

Maximizing the lifespan of wireless sensor networks is currently drawing a lot of attention in the research community. In order to reduce energy consumption, sensor nodes that are far from the base station avoid sending data directly. As a result, several disjoint clusters are formed, and nodes within a cluster send their data through the cluster head to avoid long transmissions. However, several parameters related to transmission cost need to be considered when selecting a cluster head. While most of the existing research work considers energy and distance as the most stringent parameters to reduce energy consumption, these approaches fail to create a fair and balanced cluster. Consequently, unbalanced clusters are formed, resulting in the degradation of overall performance. In this research work, a cluster head selection algorithm is proposed that covers all parts of the sensing area in a balanced manner, saving a significant amount of energy. Furthermore, a capture effect–based intracluster communication mechanism is proposed that efficiently utilizes the time slot under various traffic conditions. A Näive Bayes classifier is used to adapt the window size dynamically according to the traffic pattern. Finally, a simulation model using OMNeT++ is developed to compare the proposed approach with the pioneer clustering approach, LEACH, and the contemporary LEACH‐MAC protocol in terms of performance. The results of the simulation indicate that the proposed approach improves the overall performance in terms of network lifetime, energy efficiency, and throughput.     

即插即用模块设计乎唤创新DSP应用

灵活的马达控制设计既减少了元件数量也降低了生产成本 工程师们必须适应当今以即插即用设计策略为重点的市场环境，例如，大多数主力白色家电制造商越来越倾向于将电器子系统（甚至是关键子系统，如马达控制器）视为设备组件。     

A Multistage Architecture for Statistical Inference with Stochastic Signal Acquisition

We describe a statistical inference approach for designing signal acquisition interfaces and inference systems with stochastic devices. A signal is observed by an array of binary comparison sensors, such as highly scaled comparators in an analog-to-digital converter, that exhibit random offsets in their reference levels due to process variations or other uncertainties. These offsets can limit the performance of conventional measurement devices. In our approach, we build redundancy into the sensor array and use statistical estimation techniques to account for uncertainty in the observations and produce a more reliable estimate of the acquired signal. We develop an observational model and find a Cramér-Rao lower bound on the achievable square error performance of such a system. We then propose a two-stage inference architecture that uses a coarse estimate to select a subset of the sensor outputs for further processing, reducing the overall complexity of the system while achieving near-optimal performance. The performance of the architecture is demonstrated using a simulated prototype for parameter estimation and symbol detection applications. The results suggest the feasibility of using unreliable components to build reliable signal acquisition and inference systems.