Characterization of homofermentative lactobacilli isolated from kefir grains: potential use as probiotic

Considering that several health promoting properties are associated with kefir consumption and a reliable probiotic product requires a complete identification of the bacterial species, the present work evaluates several proved markers of probiotic potential of eleven isolates of homofermentative lactobacilli isolated from kefir grains and molecular identification and genotypic diversity. Using restriction analysis of amplified ribosomal DNA (ARDRA) and analysis of the 16S-23S rRNA internal spacer region we confirmed that all homofermentative lactobacilli belong to the species Lactobacillus plantarum. RAPD-PCR analysis allowed the discrimination of lactobacilli in five clusters. All isolates exhibited high resistance to bile salt. High survival after one hour of exposure to pH 2.5 was observed in Lb. plantarum CIDCA 8313, 83210, 8327 and 8338. All isolates were hydrophilic and non autoaggregative. Isolate CIDCA 8337 showed the highest percentage of adhesion among strains. All tested lactobacilli had strong inhibitory power against Salmonella typhimurium and Escherichia coli. Seven out of eleven isolates showed inhibition against Sal. enterica and five isolates were effective against Sal. gallinarum. Only CIDCA 8323 and CIDCA 8327 were able to inhibit Sal. sonnei. We did not find any correlation between the five clusters based on RAPD-PCR and the probiotic properties, suggesting that these isolates have unique characteristics.     

Polysaccharide production by kefir grains during whey fermentation

Fermentation of deproteinised whey with kefir grains CIDCA AGK1 was studied focusing on polysaccharide production from lactose. Kefir grains were able to acidify whey at different rates depending on the grain/whey ratio. During fermentation, kefir grains increased their weight and a water-soluble polysaccharide was released to the media. Exopolysaccharide concentration increased with fermentation time, reaching values of 57.2 and 103.4 mg/l after 5 days of fermentation in cultures with 10 and 100 g kefir grains/l, respectively. The polysaccharide fraction quantified after fermentation corresponded to the soluble fraction, because part of the polysaccharide became a component of the grain. Weight of kefir grains varied depending on the time of fermentation. Polysaccharide production was affected by temperature. Although the highest concentration of polysaccharide in the media was observed at 43 degrees C at both grain/whey ratios, the weight of the grains decreased in these conditions. In conclusion, kefir grains were able to acidify deproteinised whey, reducing lactose concentration, increasing their weight and producing a soluble polysaccharide.     

Status and Potential of Spate Irrigation in Ethiopia

This article discusses spate irrigation in Ethiopia and aims to take stock of the current status of spate irrigation development. It summarizes experiences so far and formulates a number of recommendations on the development of this upcoming resource management system. It argues that raised weirs are useful mainly in areas where a large head for spate flow diversion is required, but that traditional earthen structures with conical stone/gabion reinforcements are cost-effective and technically adequate for floodwater distribution and management. It contends that the practical successes of sediment settling ponds (gravel traps) are at best mixed. Even where a small basin is justified, it can only ever trap a small proportion of the incoming sediment load. It is better to allow large floods with excessive sediment load (>100,000 ppm) to by-pass upstream intakes to be diverted lower down the system, and provide additional livelihood opportunities in downstream areas in the form of rangeland and agro-forestry development. This is particularly useful in the lowlands of Ethiopia with large pastoral community where spate irrigation is yet to make in-roads. The article further explains that water rights in spate are different from the sharing and allocation of perennial flows—they are dynamic and respond to a situation that differs from year to year as well as within a year and that a certain degree of inequity among users is inevitable. Keeping the command area compact can ensure two or more irrigation turns and this can highly increase productivity as crops are no longer in the ‘stress zone’. To transform spate irrigation in Ethiopia from subsistence to a business-oriented production system, the article proposes the promotion of cash crops including pulses and oil seeds as well as encouraging investors to go for bio-fuel development and agro-forestry in the lowland areas where huge potential exist. To avoid vehement conflicts that may arise among the settled agricultural and pastoral communities due to the implementation of the business-oriented production system, the traditional rights of the pastoral communities must be respected and their rangeland and water resources be safeguarded. For maximum yield, soil moisture conservation measures such as pre-irrigation land preparation, deep ploughing and mulching are essential as is the conjunctive use of spate flow and groundwater. Last, but most important, the article emphasizes that farmers need to be placed at the heart of any spate irrigation development programme as primary beneficiaries, managers and operators as well as part of the decision making institution.     

Mathematical model to identify nitrogen variability in large rivers

The river Swale in Yorkshire, northern England has been the subject of many studies concerning water quality. This paper builds on existing data resources and previous 1D river water quality modelling applications at daily resolution (using QUESTOR) to provide a different perspective on understanding pollution, through simulation of the short‐term dynamics of nutrient transport along the river. The two main objectives are (1) building, calibration and evaluation of a detailed mathematical model (Advection‐Dispersion Model: ADModel), for nutrient transport under unsteady flow conditions and (2) the development of methods for estimating key parameters characterizing pollutant transport (velocity, dispersion coefficient and transformation rates) as functions of hydrological parameters and/or seasonality. The study of ammonium and nitrate has highlighted temporal variability in processes, with maximum nitrification and denitrification rates during autumn. Results show that ADModel is able to predict the main trend of measured concentration with reasonable accuracy and accounts for temporal changes in water flow and pollutant load along the river. Prediction accuracy could be improved through more detailed modelling of transformation processes by taking into account the variability of factors for which existing data were insufficient to allow representation. For example, modelling indicates that interactions with bed sediment may provide an additional source of nutrients during high spring flows. Copyright © 2010 John Wiley & Sons, Ltd.     

A numerical study of vortex interactions with flames developing from ignition kernels in lean methane/air mixtures

In this work, the outcomes of interactions of counter-rotating vortex pairs with developing ignition kernels are studied. The conditions are selected to represent those in a lean-burn natural-gas engine with hot-jet ignition. The evolution of flame surface area during kernel–vortex interaction is quantitatively and qualitatively examined. It is observed that flame development is accelerated and the net flame surface area growth rate, i.e. heat release rate, increased with increasing vortex velocity. In general, increasing the vortex length scale increases the surface growth rate, i.e. increases heat release rates, but for small length scales, i.e. when the ratio of vortex length scale to kernel diameter is small, high flame curvature induced during the interaction leads to flame weakening and slower growth rates. When the vortex velocity is high relative to the flame speed and the length scale is comparable to the kernel diameter, the vortex breaks through the ignition kernel carrying with it hot products of combustion. This accelerates growth of the flame surface area and heat release rates compared to a kernel with no vortex interaction. On decreasing the vortex velocity and increasing the length scale, the wrinkling of the kernel becomes important. This also results in increased surface growth rates and higher heat release rates.     

Exploratory studies of modeling approaches for hydrogen triple flames

A review of triple flame modeling is first presented, which demonstrates the need for additional work in this area. Building on previous methods described in the literature, a hybrid model that uses a weighted average of one-dimensional premixed and diffusion flamelet reaction rates has been proposed and evaluated for a hydrogen triple flame. Results indicated that some type of progress variable is needed for application of the diffusion flamelet contribution. Weighting the premixed flamelet reaction rate contribution at 100%, it is shown that peak temperatures between the model and a case employing detailed chemistry vary 7.5%, while heat release rate, flame speed, and mass fraction contours agree well.A second model, based on a library of reaction rates built from numerical studies which directly resolve the propagating triple flame has also been tested. Computational time for the baseline case is shown to be reduced by a factor of 3 ½ in comparison to use of detailed chemistry. The role of scalar dissipation rate as a necessary independent variable to the library has also been investigated using simulations with variable mixing layer thicknesses. Overall, it is found that large changes in local mixture fraction gradient cause rather small changes in propagation speed and total heat release rate of the hydrogen triple flame. This implies that such a model may be useful for CFD simulations that do not employ spatial resolution capable of resolving the triple flame itself.     

Design and optimization of a single stage centrifugal compressor for a solar dish-Brayton system

According to the requirements of a solar dish-Brayton system,a centrifugal compressor stage with a minimum total pressure ratio of 5,an adiabatic efficiency above 75% and a surge margin more than 12% needs to be designed.A single stage,which consists of impeller,radial vaned diffuser,90° crossover and two rows of axial stators,was chosen to satisfy this system.To achieve the stage performance,an impeller with a 6:1 total pressure ratio and an adiabatic efficiency of 90% was designed and its preliminary geometry came from an in-house one-dimensional program.Radial vaned diffuser was applied downstream of the impeller.Two rows of axial stators after 90° crossover were added to guide the flow into axial direction.Since jet-wake flow,shockwave and boundary layer separation coexisted in the impeller-diffuser region,optimization on the radius ratio of radial diffuser vane inlet to impeller exit,diffuser vane inlet blade angle and number of diffuser vanes was carried out at design point.Finally,an optimized centrifugal compressor stage fulfilled the high expectations and presented proper performance.Numerical simulation showed that at design point the stage adiabatic efficiency was 79.93% and the total pressure ratio was 5.6.The surge margin was 15%.The performance map including 80%,90% and 100% design speed was also presented.     

The Design of Cold Plates for the Thermal Management of Electronic Equipment

Cold plates, devices used for the thermal management of electronic equipment, consist of a fluid flow space that is bounded by metallic walls. The fluid passages are designed to optimize the heat extraction from the electronics. This paper deals with the fluid flow and heat transfer in cold plates in which both the fluid flow and heat transfer experience periodic variations in the streamwise direction. The motivation for the work was to devise a methodology for dealing with problems that are highly complex and also computationally demanding. The first goal of the work was to transform the combined problems of fluid flow and conjugate heat transfer into one in which the wall heat transfer can be solved separately. The decoupling was achieved by first focusing on the solution of the full conjugate heat transfer problem for a portion of the periodic array. From this solution, heat transfer coefficients were extracted and subsequently employed for the solution of the wall heat conduction problem for the entire cold plate. The second focus was the development of enhancements of the heat transfer performance of cold plates. Consideration was given to manufacturing as well as thermal and fluid flow issues.     

Analysis of Fin-Tube Joints in a Compact Heat Exchanger

This article deals with an analysis of fin-tube joints as functions of topological alterations of the joint fillet size. Based on numerical predictions of a joint topology formed by the surface tension driven reactive flow of molten metal, and subsequently verified by empirical evidence gathered through both laboratory and industrial testing, the topology alterations were identified for thermal integrity studies. Subsequently, thermal characteristics of corresponding fin-tube joints were determined in terms of two models of the thermal contact resistance. Model predictions of the fin efficiency with an altered topology of the joint zone were compared with the simulation results from a computational fluid dynamics study, and the results fit well. Numerical predictions of joint topology were devised using an in-house-developed finite-element code, and verified by the Surface Evolver code. Such prediction provided quantitative joint topology information that was needed in assessments of the joint thermal performance. Experimental data were obtained using a computer-controlled transparent hot zone with an ultra-high-purity nitrogen background atmosphere under tightly controlled conditions, and also by an analysis of the state-of-the-art manufacturing process data obtained from an industrial setting. It is demonstrated that a value of fin efficiency, assumed as recommended by traditional sizing design procedures, may drastically differ from actual values.     

THERMAL CONDUCTIVITY OF INDIUM PHOSPHIDE-BASED SUPERLATTICES

Semiconductor superlattice structures have shown promise as thermoelectric materials for their high power factor and low thermal conductivity. While the power factor of a superlattice can be controlled through band gap engineering and doping, prediction and control of thermal conductivity has remained a challenge. The thermal conductivity of three different InP/InGaAs superlattices was measured to be between 4 and 9 W/m-K from 77-320 K using the 3! method. Although the thermal conductivity of InP is an order of magnitude higher than that of InGaAs, we report the intriguing observation that as the fraction of InP is increased in InP/InGaAs superlattices, the thermal conductivity decreases. For one superlattice, the thermal conductivity was even below that of InGaAs. These observations are contrary to predictions of effective thermal conductivity by the Fourier law.