全文获取类型
收费全文 | 6393篇 |
免费 | 488篇 |
国内免费 | 11篇 |
专业分类
电工技术 | 58篇 |
综合类 | 10篇 |
化学工业 | 2827篇 |
金属工艺 | 78篇 |
机械仪表 | 86篇 |
建筑科学 | 263篇 |
矿业工程 | 35篇 |
能源动力 | 161篇 |
轻工业 | 1130篇 |
水利工程 | 41篇 |
石油天然气 | 35篇 |
无线电 | 261篇 |
一般工业技术 | 939篇 |
冶金工业 | 322篇 |
原子能技术 | 15篇 |
自动化技术 | 631篇 |
出版年
2024年 | 16篇 |
2023年 | 112篇 |
2022年 | 668篇 |
2021年 | 774篇 |
2020年 | 231篇 |
2019年 | 212篇 |
2018年 | 255篇 |
2017年 | 208篇 |
2016年 | 264篇 |
2015年 | 224篇 |
2014年 | 271篇 |
2013年 | 450篇 |
2012年 | 374篇 |
2011年 | 412篇 |
2010年 | 276篇 |
2009年 | 274篇 |
2008年 | 276篇 |
2007年 | 243篇 |
2006年 | 214篇 |
2005年 | 153篇 |
2004年 | 139篇 |
2003年 | 113篇 |
2002年 | 99篇 |
2001年 | 55篇 |
2000年 | 47篇 |
1999年 | 55篇 |
1998年 | 35篇 |
1997年 | 42篇 |
1996年 | 43篇 |
1995年 | 36篇 |
1994年 | 36篇 |
1993年 | 31篇 |
1992年 | 27篇 |
1991年 | 16篇 |
1990年 | 23篇 |
1989年 | 11篇 |
1988年 | 18篇 |
1987年 | 14篇 |
1986年 | 7篇 |
1985年 | 18篇 |
1984年 | 16篇 |
1983年 | 17篇 |
1982年 | 6篇 |
1981年 | 11篇 |
1980年 | 11篇 |
1979年 | 9篇 |
1977年 | 7篇 |
1976年 | 5篇 |
1974年 | 4篇 |
1966年 | 5篇 |
排序方式: 共有6892条查询结果,搜索用时 15 毫秒
991.
Anna Zita Mehira Kamptner Christoph-Erik Mayer Hedwig Sutterlüty 《International journal of molecular sciences》2021,22(21)
Sprouty proteins are widely accepted modulators of receptor tyrosine kinase-associated pathways and fulfill diversified roles in cancerogenesis dependent on the originating cells. In this study we detected a high expression of Sprouty3 in osteosarcoma-derived cells and addressed the question of whether Sprouty3 and Sprouty1 influence the malignant phenotype of this bone tumor entity. By using adenoviruses, the Sprouty proteins were expressed in two different cell lines and their influence on cellular behavior was assessed. Growth curve analyses and Scratch assays revealed that Sprouty3 accelerates cell proliferation and migration. Additionally, more colonies were grown in Soft agar if the cells express Sprouty3. In parallel, Sprouty1 had no significant effect on the measured endpoints of the study in osteosarcoma-derived cells. The promotion of the tumorigenic capacities in the presence of Sprouty3 coincided with an increased activation of signaling as measured by evaluating the phosphorylation of extracellular signal-regulated kinases (ERKs). Ectopic expression of a mutated Sprouty3 protein, in which the tyrosine necessary for its activation was substituted, resulted in inhibited migration of the treated cells. Our findings identify Sprouty3 as a candidate for a tumor promoter in osteosarcoma. 相似文献
992.
Karen Trchounian Anna Poladyan Armen Trchounian 《International Journal of Hydrogen Energy》2017,42(10):6590-6597
Escherichia coli growth and H2 production were followed in the presence of heavy metal ions and their mixtures during glycerol or glucose fermentation at pH 5.5–7.5. Ni2+ (50 μM) with Fe2+ (50 μM) but not sole metals stimulated bacterial biomass during glycerol fermentation at pH 6.5. Ni2++Fe3+ (50 μM), Ni2 +Fe3++Mo6+ (20 μM) and Fe3++Mo6+ (20 μM) but not sole metals enhanced up to 3-fold H2 yield but Cu+ or Cu2+ (100 μM) inhibited it. At pH 7.5 stimulating effect on biomass was observed by Ni2++Fe2++Mo6+. H2 production was enhanced 2.7 fold particularly by Ni2++Fe3++Mo6+ at the late stationary growth phase. Whereas at pH 5.5 increased biomass was when Fe2++Mo6+ or Mo6+ were added. H2 yield was decreased compared with that at pH 6.5, but metal ions again enhanced it. During glucose fermentation at pH 6.5 biomass was increased by the mixtures of metal ions, and 1.2 fold increased H2 yield was observed. At pH 7.5 Ni2++Fe2+ increased biomass but Cu+ or Cu2+ had suppressing effect; Fe3++Mo6+ stimulated H2 production. At pH 5.5 biomass also was raised by Ni2++Fe2++Mo6+; H2 yield was increased upon Mo6+ and Mo6++Fe2+ or Mo6++Fe3+ additions. The results point out the importance of Ni2+, Fe2+, Fe3+ and Mo6+ and some of their combinations for E. coli bacterial growth and H2 production mostly during glycerol but not glucose fermentation and at acidic conditions (pH 5.5 and 6.5). They can be used for optimizing fermentation processes on glycerol, controlling bacterial biomass and developing H2 production biotechnology. 相似文献
993.
Karen Trchounian Satenik Mirzoyan Anna Poladyan Armen Trchounian 《International Journal of Hydrogen Energy》2017,42(38):24026-24034
The Escherichia coli BW25113 or MC4100 wild type parental strains growth and H2 production kinetics was studied in batch cultures of minimal salt medium (MSM) and peptone medium (PM) at pH of 5.5–7.5 upon glycerol (10 g L?1) fermentation and formate (0.68 g L?1) supplementation. The role of formate alone or with glycerol on growth and H2 production via hydrogenases (Hyd) was investigated in double hyaB hybC (lacking large subunits of Hyd 1 and 2), triple hyaB hybC hycE (lacking large subunits of Hyds 1-3) and sole selC (lacking formate dehydrogenase H) mutants during 24 h bacterial growth. H2 production was delayed and observed after 24 h bacterial wild type strains growth on MSM. Moreover, it reached the maximal values after 72 h growth at the pH 6.5 and pH 7.5. Biomass formation of the mutants used was inhibited ~3.5 fold compared with wild type, and H2 production was absent in hyaB hybC hycE and selC mutants upon glycerol utilization on MSM at pHs of 5.5–7.5. Formate inhibited bacterial growth on MSM with glycerol, but enhanced and recovered H2 production by hybC mutant at pH 7.5. H2 evolution was delayed at pH 7.5 in PM, but observed and stimulated at pH 6.5 upon glycerol and formate utilization in hyaB hybC mutant. H2 production was absent in hyaB hybC hycE and selC mutants upon glycerol, formate alone or with glycerol fermentation at pH 6.5 and pH 7.5; formate supplementation had no effect. The results point out E. coli ability to grow and utilize glycerol in MSM with comparably high H2 yield: as well as they suggest the key role of Hyd-3 at both pH 6.5 and pH 7.5 and the role of Hyd-2 and Hyd-4 at pH 7.5 in H2 production by E. coli during glycerol fermentation with formate supplementation. The results obtained are novel and might be useful in H2 production biotechnology development using different nutrient media and glycerol and formate as feedstock. 相似文献
994.
Four different p‐PDA–based polyimide thin films were prepared from their respective poly(amic acid)s through thermal imidization at 400°C: poly(p‐phenylene pyromellitimide) (PMDA‐PDA); poly(p‐phenylene biphenyltetra carboximide) (BPDA‐PDA); poly(p‐phenylene 3,3′,4,4′‐oxydiphthalimide) (ODPA‐PDA); and poly(p‐phenylene 4,4′‐hexafluoroisopropylidene diphthalimide) (6FDA‐PDA). Water‐sorption behaviors of polyimide films were gravimetrically investigated at 25°C and 22–100% relative humidity by using the modified electromicrobalance (Thin Film Diffusion Analyzer). The diffusion coefficients of water for the polyimides varies in the range of 1.6 to 10.5 × 10−10 cm2/s, and are in the increasing order: BPDA‐PDA < PMDA‐PDA ∼ ODPA‐PDA < 6FDA‐PDA. The water uptakes of polyimides vary from 1.46 to 5.80 wt %, and are in the increasing order: BPDA‐PDA < ODPA‐PDA < 6FDA‐PDA < PMDA‐PDA. The water‐sorption behaviors for the p‐PDA–based polyimides are closely related to the morphological structure; specifically, the diffusion coefficients in p‐PDA–based polyimide thin films are closely related to the in‐plane orientation and mean intermolecular distance, whereas the water uptakes are affected by the packing order. © 2000 John Wiley & Sons, Inc. J Appl Polym Sci 76: 1315–1323, 2000 相似文献
995.
Bernard Martel Philippe Le Thuaut Gregorio Crini Michel Morcellet Anna‐Maria Naggi Ulrich Maschke Sabrina Bertini Carmen Vecchi Xavier Coqueret Giangiacomo Torri 《应用聚合物科学杂志》2000,78(12):2166-2173
A novel method for the preparation of immobilized α, β, or γ‐cyclodextrins on polypropylene nonwoven supports has been previously presented. The obtained new materials were prepared by graft‐polymerization of glycidyl methacrylate onto polypropylene after activation of the support by the electron beam technique, followed by the coupling of cyclodextrins with the epoxide groups. The structure of the resulting materials is characterized in detail using Fourier transform infrared spectroscopy, solid state nuclear magnetic resonance analysis, differential scanning calorimetry, thermogravimetric analysis, and optical microscopy. © 2000 John Wiley & Sons, Inc. J Appl Polym Sci 78: 2166–2173, 2000 相似文献
996.
Tomasz Jan Kolanowski Weronika Wargocka-Matuszewska Agnieszka Zimna Lukasz Cheda Joanna Zyprych-Walczak Anna Rugowska Monika Drabik Micha Fiedorowicz Seweryn Krajewski ukasz Steczek Cezary Kozanecki Zbigniew Rogulski Natalia Rozwadowska Maciej Kurpisz 《International journal of molecular sciences》2021,22(22)
997.
Anna Pierzchliska Marek Dro
dzik Monika Biaecka 《International journal of molecular sciences》2021,22(22)
Parkinson’s disease (PD) is the second most common neurodegenerative disease characterised by both motor- and non-motor symptoms, including cognitive impairment. The aetiopathogenesis of PD, as well as its protective and susceptibility factors, are still elusive. Neuroprotective effects of 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) reductase inhibitors—statins—via both cholesterol-dependent and independent mechanisms have been shown in animal and cell culture models. However, the available data provide conflicting results on the role of statin treatment in PD patients. Moreover, cholesterol is a vital component for brain functions and may be considered as protective against PD. We present possible statin effects on PD under the hypothesis that they may depend on the HMG-CoA reductase gene (HMGCR) variability, such as haplotype 7, which was shown to affect cholesterol synthesis and statin treatment outcome, diminishing possible neuroprotection associated with HMG-CoA reductase inhibitors administration. Statins are among the most prescribed groups of drugs. Thus, it seems important to review the available data in the context of their possible neuroprotective effects in PD, and the HMG-CoA reductase gene’s genetic variability. 相似文献
998.
Sara Nava Daniela Lisini Simona Frigerio Anna Bersano 《International journal of molecular sciences》2021,22(22)
Dendritic cells (DCs) are immune specialized cells playing a critical role in promoting immune response against antigens, and may represent important targets for therapeutic interventions in cancer. DCs can be stimulated ex vivo with pro-inflammatory molecules and loaded with tumor-specific antigen(s). Protocols describing the specific details of DCs vaccination manufacturing vary widely, but regardless of the employed protocol, the DCs vaccination safety and its ability to induce antitumor responses is clearly established. Many years of studies have focused on the ability of DCs to provide overall survival benefits at least for a selection of cancer patients. Lessons learned from early trials lead to the hypothesis that, to improve the efficacy of DCs-based immunotherapy, this should be combined with other treatments. Thus, the vaccine’s ultimate role may lie in the combinatorial approaches of DCs-based immunotherapy with chemotherapy and radiotherapy, more than in monotherapy. In this review, we address some key questions regarding the integration of DCs vaccination with multimodality therapy approaches for cancer treatment paradigms. 相似文献
999.
Valentina Latina Giacomo Giacovazzo Pietro Calissano Anna Atlante Federico La Regina Francesca Malerba Marco DellAquila Egidio Stigliano Bijorn Omar Balzamino Alessandra Micera Roberto Coccurello Giuseppina Amadoro 《International journal of molecular sciences》2021,22(22)
Tau cleavage plays a crucial role in the onset and progression of Alzheimer’s Disease (AD), a widespread neurodegenerative disease whose incidence is expected to increase in the next years. While genetic and familial forms of AD (fAD) occurring early in life represent less than 1%, the sporadic and late-onset ones (sAD) are the most common, with ageing being an important risk factor. Intracerebroventricular (ICV) infusion of streptozotocin (STZ)—a compound used in the systemic induction of diabetes due to its ability to damage the pancreatic β cells and to induce insulin resistance—mimics in rodents several behavioral, molecular and histopathological hallmarks of sAD, including memory/learning disturbance, amyloid-β (Aβ) accumulation, tau hyperphosphorylation, oxidative stress and brain glucose hypometabolism. We have demonstrated that pathological truncation of tau at its N-terminal domain occurs into hippocampi from two well-established transgenic lines of fAD animal models, such as Tg2576 and 3xTg mice, and that it’s in vivo neutralization via intravenous (i.v.) administration of the cleavage-specific anti-tau 12A12 monoclonal antibody (mAb) is strongly neuroprotective. Here, we report the therapeutic efficacy of 12A12mAb in STZ-infused mice after 14 days (short-term immunization, STIR) and 21 days (long-term immunization regimen, LTIR) of i.v. delivery. A virtually complete recovery was detected after three weeks of 12A12mAb immunization in both novel object recognition test (NORT) and object place recognition task (OPRT). Consistently, three weeks of this immunization regimen relieved in hippocampi from ICV-STZ mice the AD-like up-regulation of amyloid precursor protein (APP), the tau hyperphosphorylation and neuroinflammation, likely due to modulation of the PI3K/AKT/GSK3-β axis and the AMP-activated protein kinase (AMPK) activities. Cerebral oxidative stress, mitochondrial impairment, synaptic and histological alterations occurring in STZ-infused mice were also strongly attenuated by 12A12mAb delivery. These results further strengthen the causal role of N-terminal tau cleavage in AD pathogenesis and indicate that its specific neutralization by non-invasive administration of 12A12mAb can be a therapeutic option for both fAD and sAD patients, as well as for those showing type 2 diabetes as a comorbidity. 相似文献