Degradative enzymatic activities in fresh-cut blood-orange slices during chilled-storage

Blood-orange fruits are suitable to fresh-cut fruit production because of their chemical compositions. Nevertheless, the main limitation of using freshly cut oranges is their susceptibility to juiciness loss and ascorbic acid degradation because of enzymatic alterations. The aim of this work is: to identify some of the enzymes causing the qualitative decay in blood-orange slices during 15 days of chilled storage (at 4 ± 0.5 °C and 85% RH); to investigate the susceptibility to the previous alterations of five blood-orange clones ( Moro nucellare, Sanguinello nucellare , Tarocco arcimusa, Tarocco gallo and Tarocco meli ) to select the most suitable one for fresh-cut production. The enzymes studied were: pectinmethylesterase (PME) as index of juiciness loss, ascorbate oxidase (AAO) as index of ascorbic acid's degradation and polyphenol oxidase (PPO) as browning index. As far as we know, the changes of AAO activity during chilled storage of blood-orange fresh-cut slices has not previously reported and studied. Different clones showed different enzymatic activities and quality changes during chilled-storage. In particular a low juiciness loss in orange slices was correlated with a lower PME activity, as described in T. meli clone, while a high degradation of ascorbic acid was correlated with an higher AAO activity, as described in T. gallo clone; PPO activity seemed to have no significant action in quality degradation. Tarocco meli was the most suitable clone to the fresh-cut blood-orange production because it has the lowest enzymatic activity (PME, PPO and AAO) and the highest sensorial quality.     

Langmuir-blodgett films of stilbazole complexes of iridium(I) and rhodium(I)

Complexes of 4-alkoxystilbazoles with iridium and rhodium form stable Langmuir layers at the air-water interface even when the alkoxy chain is relatively short (C₅–C₁₂). The surface pressure-area isotherms indicate that condensed molecular monolayers are obtained. The area per molecule of each compound in its monolayer form is typically 0.60 nm², which agrees well with the cross-sectional area of the [Ir(CO)₂CI] or [Rh(CO)₂CI] head group predicated using molecular models. This suggests that the molecules are oriented with the metal moiety close to the water surface and their alkoxystilbazole ‘rod’ protruding from the plane of the water surface. Such floating monolayers have been transferred on to solid substrates such as glass, aluminium (AI₂O₃/AI/Glass) and silicon (SiO₂/Si) at relatively high speed (10 mm min ^?1) to form Y-type LB assemblies. The UV–Visible absorption properties of these materials in solution and LB film form have been studied. LB films of these complexes yield bathochromically shifted spectra relative to the LB film spectrum of the uncomplexed stilbazole. Additionally, these spectra are often broader and hypsochromically shifted relative to their corresponding solution spectra as a result of the close molecular packing within the LB film and the associated dipole–dipole interactions. The electrically polar nature of the molecules described in this paper suggest that they may be suitable candidates for new pyroelectric materials. Thus the pyroelectric coefficient (the rate of change of electric polarisation with respect to temperature) has been measured for a polar multilayer LB film containing an iridium complex. A pyroelectric coefficient of 3.5 μCm^?2K^?1 (at 30 °C) has been measured, which is one of the highest reported valued for an LB film. Additionally, a low dielectric loss of around 0.01 has been found over the frequency range 50 Hz–1 kHz, indicating that such LB films may be usfeul materials for pyroelectric sensors.     

Introduction to the special issue on personality and psychopathology.

The goal of this Special Issue is to enhance this reintegration of normal-range personality and abnormal psychology, 30 years after they were split apart. The articles in the Issue examine the topic from a variety of approaches, but each of them addresses the central problem of how normal-range individual differences are related to abnormal behavior. Seven of these articles examine personality in relation to specific types of psychopathology: personality disorders, alcoholism and antisocial personality, mood and anxiety disorders, dissociative disorders, somatoform disorders, eating disorders, and schizophrenia. It is impossible to summarize the wealth of findings that are contained in these articles; each deserves to be examined carefully. However, we can note some general conclusions here. First, it is abundantly clear that personality traits and psychopathological disorders are, in fact, empirically related. Second, although the observed relations tend to be orderly and psychologically meaningful, they also are relatively nonspecific. That is, one does not see a one-to-one correspondence between a given trait and a specific disorder; rather, each of the extensively studied traits is associated with several diagnostic categories. Third, due to a paucity of relevant data, the nature of these observed relations is still largely unclear. Several different explanatory models have been proposed, and each has received at least suggestive support in the literature. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

"Training group members to set session agendas: Effects on in-session behavior and member outcome": Correction to Kivlighan, Jauquet, Hardie, Francis, and Hershberger.

Reports an error in "Training group members to set session agendas: Effects on in-session behavior and member outcome" by Dennis M. Kivlighan, Carol A. Jauquet, Anne W. Hardie, Anna Maria Francis and Bernard Hershberger (Journal of Counseling Psychology, 1993[Apr], Vol 40[2], 182-187). In Table 4 (p. 186) the values for the means and standard deviations in the second, third, and fourth rows of the "Agenda setting" column were transposed with those in the "No contact" column. The corrected table is presented in this erratum. (The following abstract of the original article appeared in record 1993-26598-001.) Addressed I. D. Yalom's (1983) hypothesis that group members who set session agendas would participate more effectively in group sessions and have enhanced outcomes. The independent variable, agenda, was manipulated by assigning the 24 members of established personal growth groups to 1 of 3 conditions: (1) training in agenda setting, (2) stabilizing interviews, and (3) no-contact control. Group members filled out pre- and posttest measures of enactments of intimate behaviors and attitudes toward these enactments. They also filled out self- and other ratings of in-group enactments of intimate behaviors, and group leaders rated group members' intimate behavior at the end of each group session. Group members who set here-and-now session agendas enacted more in-group intimate behaviors and had better outcomes. Implications of these results and suggestions for group counseling are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Homozygous deletions at chromosome 9p21 and mutation analysis of p16 and p15 in microdissected primary non-small cell lung cancers

Loss of heterozygosity on chromosome 9p has been detected in many primary human tumors and cell lines, suggesting that this chromosomal arm harbors one or more tumor suppressor genes. The recently cloned p16 and p15 genes, mapped to 9p21, are likely candidates for such tumor suppressors. To map the deletion at chromosome 9p21 in non-small cell lung tumors, we analyzed DNA from 25 tumors and matching normal DNAs at six microsatellite markers that flank the region occupied by the p16 and p15 genes. Loss of heterozygosity of at least one microsatellite marker on chromosome 9p21 was detected in 13 (52%) of 25 tumors, including one tumor that exhibited homozygous deletion of both human IFNalpha and D9S171. Six tumors analyzed by a comparative multiplex PCR technique showed homozygous deletions of the sequence tag site marker c5.1 (within p16). Screening for mutations in p16 and p15 revealed one tumor with a non-sense mutation in exon 2 of p16, but no mutations were detected in p15 in any of the tumors. Thus, in these analyses approximately one-half of the non-small cell lung tumors had loss of heterozygosity at chromosome 9p21, and of these tumors, one-half had homozygous deletions of the region that includes p16. This appears to confirm the importance of a locus in this region critical to growth control in lung. The apparent lack of other mutations in p16 and p15 in the tumors with loss of heterozygosity leaves open the possibility of an unidentified gene in this region that may function as a tumor suppressor.     

Reading of lexically stressed words by Italian aphasic patients: A retrospective study.

This retrospective study investigated whether there is evidence of a 3rd reading mechanism in a transparent orthography such as Italian, where (nearly) all words can be read through the sublexical route but stress cannot always be assigned by orthography-to-phonology rules. The presence and frequency of stress errors in lexically stressed words in 16 aphasic patients with impaired reading comprehension of those same words was checked. Nine patients were reexamined months later. Notwithstanding impaired reading comprehension, none of the patients made stress errors at first examination. At follow-up, all patients showed improvement of reading comprehension and only 2 patients still had better preserved oral reading. The authors concluded that even in transparent orthographies such as Italian, the noninteractive dual-route model is inadequate for explaining all patterns of reading performances. In nonprogressive aphasias, reading comprehension can recover in a large number of patients, reducing the amplitude of the dissociation between reading aloud and reading comprehension and reducing the number of patients showing this dissociation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Memory for spatial layouts in relation to age and schema typicality.

The hypothesis that prior experience, specifically a knowledge-based schema for typical house layouts, can compensate for age declines in spatial memory was evaluated in 4 experiments. Old and young adults explored and subsequently recalled house layouts presented 1 room at a time on a computer screen. The findings failed to support the compensation hypothesis in that schema-relevant layouts facilitated recall equivalently for the 2 age groups. Violation of a typical house schema had a more negative effect on recall of the older group. Individual differences in spatial visualization ability explained much of the age difference in performance but not the effects of schema manipulations. It was concluded that there is age invariance in the facilitatory effects of relevant prior knowledge on spatial memory but an age-related decrease in the ability to inhibit irrelevant prior knowledge. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

THE EMBRYO OF BARLEY IN RELATION TO MODIFICATION OF THE ENDOSPERM

Differential dissection of the embryo of barley grains, followed by observation of the extent of modification and assessment of α-amylase production in the endosperm, has shown that the various organs of the embryo make the following different contributions to modification. The isolated scutellum has a very limited ability to produce α-amylase and its powers of enzyme secretion are largely restricted to the peripheral region where aleurone cells are present. The stimulus which initiates enzyme formation in the aleurone is largely derived from the nodal region of the embryo, especially from the base of the node where subsidiary rootlets are formed; the scutellum apparently lacks the means of producing this stimulus. Translocation of the enzyme-inducing hormone from embryo to aleurone takes place through the apical half of the scutellum in which vascular tissue develops; this preferred route of translocation partially accounts for the asymmetric pattern of modification normally observed. Removal of the first foliar leaf from within the coleoptile allows increased production of α-amylase in the endosperm: this leaf can be regarded as a natural consumer of gibberellin.