Activated α,β‐Unsaturated Aldehydes as Substrate of Dihydroxyacetone Phosphate (DHAP)‐Dependent Aldolases in the Context of a Multienzyme System

The utility for carbon‐carbon bond formation of a multienzyme system composed of recombinant dihydroxyacetone kinase (DHAK) from Citrobacter freundii, the fructose bisphosphate aldolase from rabbit muscle (RAMA) and acetate kinase (AK) for adenosine triphosphate (ATP) regeneration has been studied. Several aldehydes with great structural diversity, including three α,β‐unsaturated aldehydes, have been analysed as acceptor substrates. It was found that α,β‐unsaturated aldehydes bearing an electron‐withdrawing group in the β position to the double bond with a trans configuration are good acceptors for RAMA in this multienzyme system. The aldol reaction proceeds with excellent D ‐threo enantioselectivity and the aldol adduct is obtained in good overall yield. The L ‐threo and D ‐erythro enantiomers are also accessible from rhamnulose 1‐phosphate aldolase (Rha‐1PA) and fuculose 1‐phosphate aldolase (Fuc‐1PA) catalysed reactions, respectively.     

Treatment of petroleum refinery sourwater by advanced oxidation processes

The performance of several oxidation processes to remove organic pollutants from sourwater was investigated. Sourwater is a specific stream of petroleum refineries, which contains slowly biodegradable compounds and toxic substances that impair the industrial biological wastewater treatment system. Preliminary experiments were conducted, using the following processes: H2O2, H2O2/UV, UV, photocatalysis, ozonation, Fenton and photo-Fenton. All processes, except Fenton and photo-Fenton, did not lead to satisfactory results, reducing at most 35% of the sourwater dissolved organic carbon (DOC). Thus, further experiments were performed with these two techniques to evaluate process conditions and organic matter removal kinetics. Batch experiments revealed that the Fenton reaction is very fast and reaches, in a few minutes, an ultimate DOC removal of 13-27%, due to the formation of iron complexes. Radiation for an additional period of 60 min can increase DOC removal up to 87%. Experiments were also conducted in a continuous mode, operating one 0.4L Fenton stirred reactor and one 1.6L photo-Fenton reactor in series. DOC removals above 75% were reached, when the reaction system was operated with hydraulic retention times (HRT) higher than 85 min. An empirical mathematical model was proposed to represent the DOC removal kinetics, allowing predicting process performance quite satisfactorily.     

A 0.8 μm CMOS testable switched-capacitor filter for video frequency applications

This paper presents the design of a fifth-order low-pass elliptic filter that employs a parallel connection of two all-pass sections to satisfy specifications commonly used in video frequency applications. Operating with a sampling frequency of 16 MHz, the IC prototype was implemented in a standard double-poly CMOS 0.8 μm process. The experimental verification showed a passband frequency deviation smaller than 0.08 dB up to the passband edge frequency of 3.4 MHz, and an output noise power of 0.97 ${\mu {\rm V}_{\rm RMS}}/{\sqrt {Hz}}This paper presents the design of a fifth-order low-pass elliptic filter that employs a parallel connection of two all-pass sections to satisfy specifications commonly used in video frequency applications. Operating with a sampling frequency of 16 MHz, the IC prototype was implemented in a standard double-poly CMOS 0.8 μm process. The experimental verification showed a passband frequency deviation smaller than 0.08 dB up to the passband edge frequency of 3.4 MHz, and an output noise power of 0.97 , resulting in a dynamic range of 49.1 dB. The filter structure enables multiple fault detection and suits modern automated testing configurations to allow accurate estimation of the actually implemented transfer function parameters, an issue of increasing importance in VLSI circuit design. The relative area required for testing the fifth-order filter is only 8% of the total filter area, and decreases as the filter order increases. Jorge Morales Ca?ive was born in Cienfuegos, Cuba, in 1963. He received the B.Sc. and M.Sc. degrees from the Technical University of San Petersburg, Russia, in 1986 and 1988, respectively, and the D.Sc. degree from the Federal University of Rio de Janeiro, Brazil, in 1991, all in electrical engineering. From 1988 to 1994, he worked at CEADEN, in Havana, Cuba, on the development of nuclear equipments. From 1994 to 1997, he worked at INOR, in Havana, Cuba, on the research and development of acquisition systems and image processing for nuclear medicine. His research interests are in the areas of analog and digital signal processing. Antonio Petraglia (S’89-M’91-SM’99) received the Engineer and M.Sc. degrees from the Federal University of Rio de Janeiro (UFRJ), Brazil, in 1977 and 1982, respectively, and the Ph.D. degree from the University of California, Santa Barbara (UCSB), in 1991, all in electrical engineering. In 1979, he joined the Faculty of UFRJ as an Associate Professor of electrical engineering, where he served as a Co-Chair in the Department of Electronic Engineering from 1982 to 1984. During the second semester of 1991, he was a post-Doctoral researcher with the Department of Electrical and Computer Engineering at UCSB. Since 1992 he has been on the faculty of the Program for Post-Graduate Engineering at UFRJ, where in 1997 he established the Laboratory for the Processing of Analog and Digital Signals. From March 2001 through March 2002 he was a Visiting Scholar with the Electrical Engineering Department at the University of California, Los Angeles. He has been involved in teaching and research activities in the areas of analog and digital signal processing, and in mixed analog-digital integrated circuit design. He is a distinguished member of the Brazilian Millenium Group in Nanoelectronics and Microelectronics in 2006-2008. Dr. Petraglia served as an Associate Editor for the IEEE Transactions on Circuits and Systems-II: Analog and Digital Signal Processing in 2002–2003 Mariane Rembold Petraglia (M’97) received the B.Sc. degree in electronic engineering from the Federal University of Rio de Janeiro, Brazil, in 1985, and the M.Sc. and Ph.D. degrees in electrical engineering from the University of California, Santa Barbara, in 1988 and 1991, respectively. From 1992 to 1993, she was with the Department of Electrical Engineering, Catholic University of Rio de Janeiro, Brazil. Since 1993, she has been with the Department of Electronic Engineering and with the Program of Electrical Engineering, COPPE, at the Federal University of Rio de Janeiro, where she is presently an Associate Professor. From March 2001 to February 2002, she was a Visiting Researcher with the Adaptive Systems Laboratory, at the University of California, Los Angeles. Her research interests are in adaptive signal processing, multirate systems, and image processing. Dr. Petraglia is a member of Tau Beta Pi, and a distinguished member of the Brazilian Millenium Group in Nanoelectronics and Microelectronics in 2006–2008. She is serving as an Associate Editor for the IEEE Transactions on Signal Processing since Nov. 2004.     

Detection of FITC-cortisol via modulated supraparticle lighthouses

Hormones are important bioactive compounds in blood and tissue that vary in concentration in response to stress and certain disease states. Establishing the changes in physiological hormone concentrations over time can lead to more effective diagnoses and perhaps a better understanding of the evolution of stress and disease. To monitor concentration over time, the sampling must be rapid and noninvasive; specimens such as saliva that require little effort to collect are preferred. However, more sensitive assay techniques are needed when compared to blood analysis since free hormone concentration in saliva is only a small fraction of the concentration in circulating blood. In this work, magnetic field-induced structures of paramagnetic particles are used as a solid substrate to demonstrate improved detection limits for a separation-free assay of cortisol. Once formed, the structures are subjected to a rotating magnetic field and this leads to two important features. First is the ability to utilize frequency and phase filtering (lock-in amplification) for the signal generated from surface-bound labeled species. Second is the improved mass transport of the antigen to the surface of the rotating structures. These two unique capabilities result in a quantifiable signal at a relatively low target antigen concentration. This method has been demonstrated with the detection of fluorescein isothiocyanate-labeled cortisol (FITC-cortisol) at a concentration of 300 pM.     

Comments on “A new heuristic approach for optimal reconfiguration in distribution systems” in 81 (2011) 282-289

This short communication is a discussion of the paper entitled “A new heuristic approach for optimal reconfiguration in distribution systems” introduced a new approach for optimal reconfiguration of radial distribution systems. Besides, a load flow algorithm based on graph theory was presented in order to give precise branch currents, node voltages and system power losses. In the abstract and in the conclusion section of the above mentioned study, the author stated that the results show that the performance of the proposed method is better than that of several methods available in other published articles, and that the proposed technique represents an improved, more efficient method which can easily solve the distribution network reconfiguration problem compared with those other methods. However, this conclusion can not be deduced from the results given by the author because with the optimal radial configuration given for the test system used, the power losses obtained are higher than those obtained by some of the other methods. So, the proposed final solution is worse.     

Power‐efficient analog design based on the class AB super source follower

A class AB version of the conventional super source follower (SSF) is described. The circuit greatly increases slew rate (SR) and current efficiency, maintaining the low distortion and low output resistance of the SSF. Class AB operation is achieved without extra power dissipation or supply requirements, and without bandwidth or noise degradation. The circuit can advantageously replace the SSF in a wide variety of analog systems, opening a new research line in analog design. To illustrate the widespread application of this cell, a class AB differential unity‐gain buffer, a class AB differential current mirror and two class AB differential transconductors are designed, fabricated in a 0.5µm CMOS technology and tested. Measurement results using a dual supply of ±1.65V show that the proposed class AB version of the SSF improves SR by a factor 21.5 and increases bandwidth by 10%, keeping noise level, input range, power consumption, and supply requirements unaltered. The fabricated class AB current mirror features a THD at 100 kHz of ? 62dB for signal currents 20 times larger than the bias current. The fabricated transconductors feature an IM3 at 1 MHz of ? 56.6dB for output currents more than 13 times larger than the bias currents. Copyright © 2011 John Wiley & Sons, Ltd.     

Soft Nanopatterning on Light‐Emitting Inorganic–Organic Composites

In this work we demonstrate the nanopatterning of nanocomposites made by luminescent zinc oxide nanoparticles and light‐emitting conjugated polymers by means of soft molding lithography. Vertical nanofluidics is exploited to overcome the polymer transport difficulties intrinsic in materials incorporating nanocrystals, and the rheology, fluorescence, absolute quantum yield, and emission directionality of the nanostructured composites are investigated. We study the effect of patterned gratings on the directionality of light emitted from the nanocomposites, finding evidence of the enhancement of forward emitted light, due to the printed wavelength‐scale periodicity. These results open new possibilities for the realization of nanopatterned devices based on hybrid organic‐inorganic systems.     

A radius and ulna TW3 bone age assessment system

An end-to-end system to automate the well-known Tanner--Whitehouse (TW3) clinical procedure to estimate the skeletal age in childhood is proposed. The system comprises the detailed analysis of the two most important bones in TW3: the radius and ulna wrist bones. First, a modified version of an adaptive clustering segmentation algorithm is presented to properly semi-automatically segment the contour of the bones. Second, up to 89 features are defined and extracted from bone contours and gray scale information inside the contour, followed by some well-founded feature selection mathematical criteria, based on the ideas of maximizing the classes' separability. Third, bone age is estimated with the help of a Generalized Softmax Perceptron (GSP) neural network (NN) that, after supervised learning and optimal complexity estimation via the application of the recently developed Posterior Probability Model Selection (PPMS) algorithm, is able to accurately predict the different development stages in both radius and ulna from which and with the help of the TW3 methodology, we are able to conveniently score and estimate the bone age of a patient in years, in what can be understood as a multiple-class (multiple stages) pattern recognition approach with posterior probability estimation. Finally, numerical results are presented to evaluate the system performance in predicting the bone stages and the final patient bone age over a private hand image database, with the help of the pediatricians and the radiologists expert diagnoses.     

Multicast deflector

Technological evolution is leading telecommunications toward all-IP scenarios, where multiple services are transported as IP packets. Among these services is the broadcast of video. A possible mechanism for broadcasting multiple video channels over IP is to use IP multicast, and let each client decide about the reception of a channel. The secure IP multicast specified by the IETF MSEC working group is a candidate solution for securing these broadcast services. In this paper we propose a new solution for supporting the broadcast of multiple video channels which can be accessed only by authorized users; besides, when a video channel is not visualized in the last mile its transmission is temporarily suspended, so that the cable can be used for other services such as standard Internet access.