Intrinsic Images by Clustering

Decomposing an input image into its intrinsic shading and reflectance components is a long‐standing ill‐posed problem. We present a novel algorithm that requires no user strokes and works on a single image. Based on simple assumptions about its reflectance and luminance, we first find clusters of similar reflectance in the image, and build a linear system describing the connections and relations between them. Our assumptions are less restrictive than widely‐adopted Retinex‐based approaches, and can be further relaxed in conflicting situations. The resulting system is robust even in the presence of areas where our assumptions do not hold. We show a wide variety of results, including natural images, objects from the MIT dataset and texture images, along with several applications, proving the versatility of our method.     

Combustion synthesis and electrochemical characterisation of Pt–Ru–Ni anode electrocatalyst for PEMFC

The present work studies the synthesis by the combustion method of an anode catalyst for protonic exchange membrane fuel cell (PEMFC) employing two different fuels, that is, urea and sucrose. The unsupported pure solid solution Pt_0.6Ru_0.3Ni_0.1 was selected from a calculated and empirical ternary phase diagram, which was previously studied. Theoretically, this particular composition exhibited single-phase features without the presence of secondary phases as RuO₃ and NiO, regarding the oxygen partial pressure conditions generated during the combustion synthesis. In the X-ray diffraction (XRD) analysis of the nanoparticles synthesized by using two different fuels, a single-phase Pt_0.6Ru_0.3Ni_0.1 alloy was detected. However, the X-ray photoelectron spectroscopy (XPS) studies showed that the nanoparticles prepared could present an onion-shell structure, in the case of the sample synthesized with sucrose as fuel, the external layers are partially constituted by Ni hydroxides, which can exhibit an active role in the hydrogen oxidation reaction. The electrochemical behaviour of this unsupported catalyst was performed by preparing MEAs, which were evaluated using a I–V polarisation curve test. The results obtained indicated that the nanoparticles prepared by sucrose have better performance, 260 mW/cm², than those prepared using urea, 170 mW/cm². These results are discussed in relation with the hydrogen oxidation mechanism. The results obtained reveal combustion synthesis as an appropriate method for preparing PEMFC electrocatalysts, due to its versatility, simplicity and fastness.     

A Narrowing-based Instantiation Rule for Rewriting-based Fold/Unfold Transformations

In this paper we show how to transfer some developments done in the field of functionallogic programming (FLP) to a pure functional setting (FP). More exactly, we propose a complete fold/unfold based transformation system for optimizing lazy functional programs. Our main contribution is the definition of a safe instantiation rule which is used to enable effective unfolding steps based on rewriting. Since instantiation has been traditionally considered problematic in FP, we take advantage of previous experiences in the more general setting of FLP where instantiation is naturally embedded into an unfolding rule based on narrowing. Inspired by the so called needed narrowing strategy, our instantiation rule inherits the best properties of this refinement of narrowing. Our proposal optimizes previous approaches (that require more transformation effort) defined in the specialized literature of pure FP by anticipating bindings on unifiers used to instantiate a given program rule and by generating redexes at different positions on instantiated rules in order to enable subsequent unfolding steps. As a consequence, our correct/complete technique avoids redundant rules and preserves the natural structure of programs.     

Nonlinear properties of tapered laser cavities

The nonlinear phenomena accompanying the process of light generation in high-power tapered semiconductor lasers are studied using a combination of simulation and experiment. Optical pumping, electrical overpumping, filamentation, and spatial hole burning are shown to be the key nonlinear phenomena influencing the operation of tapered lasers at high output powers. In the particular tapered laser studied, the optical pumping effect is found to have the largest impact on the output beam quality. The simulation model used in this study employs the wide-angle finite-difference beam propagation method for the analysis of the optical propagation within the cavity. Quasi-three-dimensional (3-D) thermal and electrical models are used for the calculation of the 3-D distributions of the temperature, electrons, holes, and electrical potential. The simulation results reproduce key features and the experimental trends.     

Analysis of inorganic anions in drainage water and soil solution by single-column ion chromatography

Single-column ion chromatography (SCIC) for anion determination in drainage water and soil solution was tested. The SCIC minimum detection limits (100-microliter sample loop) were 0.75 mg l-1 for Cl-, 0.2 mg l-1 for NO2-N, 0.02 mg l-1 for NO3-N, 1.25 mg l-1 for HPO4-P, and 0.5 mg l-1 for SO4-S. The results showed a high reproducibility. Results for Cl-, NO3-N and SO4-S obtained by the SCIC method were compared with those obtained by traditional methods; Student's t-test and regression analysis showed that the methods agree closely.     

Modafinil Administration to Preadolescent Rat Impairs Non-Selective Attention,Frontal Cortex D2 Expression and Mesolimbic GABA Levels

The misuse of psychostimulants is an increasing behavior among young people, highlighting in some countries the abuse of modafinil (MOD) as a neuropotentiator. However, several clinical trials are investigating MOD as an alternative pharmacological treatment for attentional deficit and hyperactivity disorder (ADHD) in children and adolescents. On the other hand, the early use of psychostimulants and the misdiagnosis rates in ADHD make it crucial to investigate the brain effects of this type of drug in young healthy individuals. The aim of this work was to evaluate the effects of chronic MOD treatment on neurochemicals (γ-aminobutyric acid and glutamate), dopamine receptor 2 (D₂) expression and behavior (non-selective attention “NSA”) in the mesocorticolimbic system of young healthy Sprague–Dawley rats. Preadolescent male rats were injected with MOD (75 mg/kg, i.p.) or a vehicle for 14 days (from postnatal day 22 to 35). At postnatal day 36, we measured the GLU and GABA contents and their extracellular levels in the nucleus accumbens (NAc). In addition, the GLU and GABA contents were measured in the ventral tegmental area (VTA) and D₂ protein levels in the prefrontal cortex (PFC). Chronic use of MOD during adolescence induces behavioral and neurochemical changes associated with the mesocorticolimbic system, such as a reduction in PFC D₂ expression, VTA GABA levels and NSA. These results contribute to the understanding of the neurological effects of chronic MOD use on a young healthy brain.     

Production of human growth hormone by Lactococcus lactis

A synthetic gene coding for human growth hormone was expressed in Lactococcus lactis. The presence of the recombinant protein was assayed and quantified using ELISA tests. Human growth hormone was detected at high concentrations and displayed a biological activity similar to the one shown by commercial human growth hormone.     

Secretome from Human Mesenchymal Stem Cells-Derived Endothelial Cells Promotes Wound Healing in a Type-2 Diabetes Mouse Model

Tissue regeneration is often impaired in patients with metabolic disorders such as diabetes mellitus and obesity, exhibiting reduced wound repair and limited regeneration capacity. We and others have demonstrated that wound healing under normal metabolic conditions is potentiated by the secretome of human endothelial cell-differentiated mesenchymal stem cells (hMSC-EC). However, it is unknown whether this effect is sustained under hyperglycemic conditions. In this study, the wound healing effect of secretomes from undifferentiated human mesenchymal stem cells (hMSC) and hMSC-EC in a type-2 diabetes mouse model was analyzed. hMSC were isolated from human Wharton’s jelly and differentiated into hMSC-EC. hMSC and hMSC-EC secretomes were analyzed and their wound healing capacity in C57Bl/6J mice fed with control (CD) or high fat diet (HFD) was evaluated. Our results showed that hMSC-EC secretome enhanced endothelial cell proliferation and wound healing in vivo when compared with hMSC secretome. Five soluble proteins (angiopoietin-1, angiopoietin-2, Factor de crecimiento fibroblástico, Matrix metallopeptidase 9, and Vascular Endothelial Growth Factor) were enriched in hMSC-EC secretome in comparison to hMSC secretome. Thus, the five recombinant proteins were mixed, and their pro-healing property was evaluated in vitro and in vivo. Functional analysis demonstrated that a cocktail of these proteins enhanced the wound healing process similar to hMSC-EC secretome in HFD mice. Overall, our results show that hMSC-EC secretome or a combination of specific proteins enriched in the hMSC-EC secretome enhanced wound healing process under hyperglycemic conditions.     

Interrogating the Behaviour of a Styryl Dye Interacting with a Mesoscopic 2D-MOF and Its Luminescent Vapochromic Sensing

In this contribution, we report on the solid-state-photodynamical properties and further applications of a low dimensional composite material composed by the luminescent trans-4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran (DCM) dye interacting with a two-dimensional-metal organic framework (2D-MOF), Al-ITQ-HB. Three different samples with increasing concentration of DCM are synthesized and characterized. The broad UV-visible absorption spectra of the DCM/Al-ITQ-HB composites reflect the presence of different species of DCM molecules (monomers and aggregates). In contrast, the emission spectra are narrower and exhibit a bathochromic shift upon increasing the DCM concentration, in agreeance with the formation of adsorbed aggregates. Time-resolved picosecond (ps)-experiments reveal multi-exponential behaviors of the excited composites, further confirming the heterogeneous nature of the samples. Remarkably, DCM/Al-ITQ-HB fluorescence is sensitive to vapors of electron donor aromatic amine compounds like aniline, methylaniline, and benzylamine due to a H-bonding-induced electron transfer (ET) process from the analyte to the surface-adsorbed DCM. These findings bring new insights on the photobehavior of a well-known dye when interacting with a 2D-MOF and its possible application in sensing aniline derivatives.