An inductive characterization of matching in binding bigraphs

We analyze the matching problem for bigraphs. In particular, we present a sound and complete inductive characterization of matching in bigraphs with binding. Our results yield a specification for a provably correct matching algorithm, as needed by our prototype tool implementing bigraphical reactive systems.     

Fault‐Tolerant Control Allocation for Overactuated Nonlinear Systems

This paper addresses the problem of fault‐tolerant control allocation for input affine nonlinear systems. The proposed scheme is divided in three main tasks: fault detection and estimation using a nonlinear observer, fault isolation through a bank of unknown input observers with a resetting policy to reduce the effects of nonlinearities and control reconfiguration based on reduced order allocation. Analytical results regarding the isolability and reconfigurability of actuator faults are derived and a simulation example is used to illustrate the the proposed fault tolerant control methodology.     

Effects of transferring glacier-fed water to a clear-water mountain river on the production and food organisms of brown trout (Salmo trutta L.) in Southern Norway

The effects of transferring glacier-fed water to a clear-water river on production and food organisms of brown trout (Salmo trutta L.) were investigated in a mountain river, in southern Norway. Production in unregulated fluvial habitat was 271.5 g 100 m^?2 yr^?1 compared with 103.1 g 100 m^?2 yr^?1 in a glacier-fed reach of the river. This difference was due to low fish density and recruitment rate. The growth rate, at least for younger fish (2+ to 4+ age groups), was significantly higher in the regulated reach of the river. The main difference in food composition was the low abundance of crustaceans Eurycercus lamellatus and Gammarus lacustris in the regulated reach. Trichopterans were the main diet component in both sites.     

Nanoporous Gold Catalyst for the Oxidative N-Dealkylation of Drug Molecules: A Method for Synthesis of N-Dealkylated Metabolites

A novel method for the selective catalytic N-dealkylation of drug molecules on a nanoporous gold (NPG) catalyst producing valuable N-dealkylated metabolites and intermediates is described. Drug metabolites are important chemical entities at every stage of drug discovery and development, from exploratory discovery to clinical development, providing the safety profiles and the ADME (adsorption, distribution, metabolism, and elimination) of new drug candidates. Synthesis was carried out in aqueous solution at 80 °C using air (oxygen source) as oxidant, in single step with good isolated yields. Different examples examined in this study showed that aerobic catalytic N-dealkylation of drug molecules on NPG has a broad scope supporting N-deethylation, N-deisopropylation and N-demethylation, converting either 3° amines to 2° amines, or 2° amines to 1° amines.     

New Insights to Clathrin and Adaptor Protein 2 for the Design and Development of Therapeutic Strategies

The Amyloid Precursor Protein (APP) has been extensively studied for its role as the precursor of the β-amyloid protein (Aβ) in Alzheimer’s disease (AD). However, our understanding of the normal function of APP is still patchy. Emerging evidence indicates that a dysfunction in APP trafficking and degradation can be responsible for neuronal deficits and progressive degeneration in humans. We recently reported that the Y₆₈₂ mutation in the ₆₈₂YENPTY₆₈₇ domain of APP affects its binding to specific adaptor proteins and leads to its anomalous trafficking, to defects in the autophagy machinery and to neuronal degeneration. In order to identify adaptors that influence APP function, we performed pull-down experiments followed by quantitative mass spectrometry (MS) on hippocampal tissue extracts of three month-old mice incubated with either the ₆₈₂YENPTY₆₈₇ peptide, its mutated form, ₆₈₂GENPTY₆₈₇ or its phosphorylated form, ₆₈₂pYENPTY₆₈₇. Our experiments resulted in the identification of two proteins involved in APP internalization and trafficking: Clathrin heavy chain (hc) and its Adaptor Protein 2 (AP-2). Overall our results consolidate and refine the importance of Y₆₈₂ in APP normal functions from an animal model of premature aging and dementia. Additionally, they open the perspective to consider Clathrin hc and AP-2 as potential targets for the design and development of new therapeutic strategies.     

The Electrical Response of the Human Eye to Sinusoidal Light Stimulation

In order to extract maximum information from electroretingraphic waveforms obtained under clinical conditions, it is necessary to have reliable quantitative methods to characterize both the amplitude and shape of these waveforms. When sinusoidally modulated light is used to stimulate the retina, the resulting ERG potentials are, in general, not sinusoidal due to the nonlinearities in the system. However, the responses are very reproducible and can easily be characterized by a few parameters based on a Fourier analysis. The more conventional flash ERG, although usually of higher amplitude, is much less reproducible in shape and needs many more parameters to characterize completely. Amplitude and phase characteristics can be understood on the basis of a simple model for the scotopic B-wave system and additive interaction by the photopic system. Changes in amplitude and phase characteristics with various experimental conditions could be predicted and were confirmed by subsequent experiments. The ultimate goal of this work is to improve the quantitative basis for clinical electroretinography and to provide the clinician with additional data which can be useful in the diagnosis of retinal diseases.     

Conversion gain of m.e.s.f.e.t. drain mixers

A theoretical analysis of the gain properties of m.e.s.f.e.t. drain mixers is presented. The m.e.s.f.e.t. model includes the nonlinearity of both the transconductance and the drain resistance. For a special case, a simple analytical expression for the gain is given. Numerical results for a typical example are briefly presented as an illustration.