Automated parameter estimation of the Hodgkin-Huxley model using the differential evolution algorithm: application to neuromimetic analog integrated circuits

We propose a new estimation method for the characterization of the Hodgkin-Huxley formalism. This method is an alternative technique to the classical estimation methods associated with voltage clamp measurements. It uses voltage clamp type recordings, but is based on the differential evolution algorithm. The parameters of an ionic channel are estimated simultaneously, such that the usual approximations of classical methods are avoided and all the parameters of the model, including the time constant, can be correctly optimized. In a second step, this new estimation technique is applied to the automated tuning of neuromimetic analog integrated circuits designed by our research group. We present a tuning example of a fast spiking neuron, which reproduces the frequency-current characteristics of the reference data, as well as the membrane voltage behavior. The final goal of this tuning is to interconnect neuromimetic chips as neural networks, with specific cellular properties, for future theoretical studies in neuroscience.     

A comparative study of manganese and lead levels in human umbilical cords and maternal blood from two urban centers exposed to different gasoline additives

Manganese (Mn) and lead (Pb) are two neurotoxic chemicals and experimental evidence suggests that they can cross the placental barrier. Tetraethyl lead was still in use as an antiknock agent in Paris during the sampling period of the study, while it has been replaced by methylcyclopentadienyl manganese tricarbonyl (MMT) in Canada since 1977. By 1990, MMT was in 100% of gasoline in Canada. In a study of 160 pairs of mothers-neonates in Montreal and 206 pairs in Paris, we compared levels of Mn and Pb in the umbilical cord and in maternal blood. Neonates and mothers had significantly higher Pb levels in Paris where lead additives were still used in gasoline. Geometric mean maternal blood Pb levels were 5.4 microg/dl compared to 2.1 microg/dl in Montreal and cord blood Pb levels were 3.2 microg/dl in Parisian mothers compared to 1.7 microg/dl in Montreal. The prevalence of Paris Pb values superior to the 95th percentile of the Montreal distribution was highly elevated in all media studied. The prevalence of high Mn levels in umbilical cord blood was also significantly higher in Montreal. Surveillance programs are important to limit Pb overexposure and associated neurological effects in neonates where tetraethyl Pb is still in use as a gasoline additive. Since Mn is an essential element and dietary Mn intake may differ between Montreal and Paris, the difference observed with regard to high Mn values between Montreal and Paris cannot, at this time, be attributed to MMT in Montreal's gasoline. Further studies are needed to infer an association between Mn emissions from MMT and prenatal exposure to Mn.     

Information visualization for selection in Design by Shopping

In Design by Shopping, designers explore the design space to gain an insight into trades, and feasible and impractical solutions, as well as to learn about alternatives before optimization and selection. The design space consists of multidimensional sets of data and, in order to select the best design from among numerous alternatives, designers may use several different graphs. In this study, we test to find the most appropriate graph to indicate the best solution corresponding to a set of objectives represented by a design scenario (1). A further constraint is that this must be done in the shortest possible time (2). Three graph types are tested in three different design scenarios where one car has to be chosen from a total of 40. A response quality index is proposed which computes the quality of a designer’s choice for any given scenario. In total, 90 tests with 30 participants were performed. The parallel coordinates plot proved to be the best graph for selection in Design by Shopping.     

Formation Control of Agricultural Mobile Robots: A Bidirectional Weighted Constraints Approach

This paper focuses on the formation control of several mobile robots in off‐road conditions. A control strategy based on bidirectional referencing is proposed, where each robot combines a velocity control w.r.t. the immediate preceding neighbor with a control w.r.t. the immediate following neighbor. Two virtual leaders, respectively, at the head and at the tail of the fleet, are propelling the fleet at the desired velocity. The fleet stability is investigated using Lyapunov techniques, pending on combination coefficients. The stability is theoretically proven for constant coefficients as weights of the respective velocity controls and tested through simulations and full‐scale experiments. It is shown that this control strategy permits us to reduce the error propagation problem often encountered in formation control, while limiting the communication problems of the global strategies. As a result, a stable formation control architecture is defined, which requires each vehicle to be refereed only to two other robots. This limited communication need is particularly interesting for agricultural operations. The proposed approach is implemented in this paper on agricultural tractors.     

Influence of hydrothermally-synthesized LaPO4:Tb nanorods on the physical and physico-chemical properties of photo-structured acrylate material

This paper presents some encouraging features on Tb³⁺-doped LaPO₄, used for the first time as nanoparticles (NPs) for photo-structured composite material. First, LaPO₄:Tb³⁺ nanorods are hydrothermally synthesized. TEM analysis point out that their size distribution and their morphology are well defined. Then, a small amount of NPs (2 or 3.9 wt%) is dispersed in acrylate monomers mixture and the photo-patterning is performed by using holography, a technique among the others. It is interesting to note that even if the loading of the NPs is weak, these last ones significantly influence the physical properties of the gratings. Indeed, the observed increase in the refractive index modulation depth is ascribed to both phenomena: the sequestration of LaPO₄:Tb³⁺ nanorods within the periodic structure and the change in the kinetics of photopolymerization. The localization of NPs in the non-illuminated zones, evidenced by fluorescence microscopy, is favoured by the decrease in the rate of polymerization, revealed by infrared spectroscopy.     

Comparison of Age-dependent Quantitative Changes in the Male Labial Gland Secretion of <Emphasis Type="Italic">Bombus Terrestris</Emphasis> and <Emphasis Type="Italic">Bombus Lucorum</Emphasis>

Age-related changes of antennal-active components of male labial gland extracts were studied in two closely related bumblebee species, Bombus terrestris and B. lucorum. In B. terrestris, compounds eliciting electroantennogram (EAG) responses of virgin queens were ethyl dodecanoate, 2,3-dihydrofarnesal, 2,3-dihydrofarnesol, hexadecan-1-ol, octadeca-9,12,15-trien-1-ol, and geranylcitronellol. Compounds that elicited EAG responses from queens of B. lucorum were ethyl dodecanoate, ethyl tetradec-7-enoate, ethyl tetradec-9-enoate, ethyl hexadec-9-enoate, hexadecan-1-ol, hexadec-7-enal, octadeca-9,12-dien-1-ol, octadeca-9,12,15-trien-1-ol, and octadecan-1-ol. Quantities of these compounds in the labial glands changed significantly over the lifetime of the respective males of the two species. In both species, concentrations of the respective compounds reached their maximum within seven days after eclosion. Subsequently, a rapid decrease in the amount of EAG-active compounds occurred in B. terrestris, whereas in B. lucorum the amount of active compounds stayed approximately constant or decreased at a slow rate. Microscopy showed that in B. terrestris secretory cells of the labial glands undergo apoptosis from the fifth to the tenth day of life, whilst in B. lucorum labial gland cells remain unchanged throughout the life of the males.     

The built environment and health: Impacts of pedestrian-friendly designs on air pollution exposure

In the wake of the growing popularity of pedestrian-oriented community designs, it is timely to assess potential risk trade-offs of such urban planning strategies. Pedestrian-friendly designs are currently being called for and implemented in the US to tackle in particular problems associated with insufficient physical activity in the population. Unintended consequences may emerge, however, especially due to potential increases in the inhalation of pollutants as the population walking or cycling in polluted environments increases. A risk assessment of such built environment transformations was undertaken to evaluate quantitatively the competing risks and benefits of community design changes in active travel. A simulation model, built incorporating research from the fields of transportation, environmental sciences and exposure analysis, is applied to a case study area that undergoes hypothetical urban transformations. We find that the simulated population experiences roughly the same number of days in a year with decreases as number of days with increases in energy expenditure or inhalation of pollutants. In the 5% of days with greatest shifts, PM₁₀ inhalation was shown to increase by 175% or more, while the 5% of days of greatest decreases exhibited reductions of 45% or more (with similar results for ozone). Of particular concern, some individuals are shown to double their intake of the pollutants on high pollution days. However, uncertainty in the estimates is high. In particular, interpretations are very different according to the approach used to characterize year-long activity patterns. This innovative risk assessment uncovers critical gaps in the literature that must be further researched to allow essential comprehensive analyses of planning decisions.     

Evaluation of the reliability and validity of GraphClick as a data extraction program

Data extraction programs are used to obtain approximations of raw data from published graphs. Quantitative syntheses of single-subject data require that researchers have access to valid and reliable data extraction programs. We analyzed the reliability and validity of a data extraction program, GraphClick. Three independent coders extracted data from 191 graphs from the Journal of Applied Behavior Analysis (JABA) and 15 graphs of hypothetical data. Results showed that GraphClick is both reliable and valid with nearly perfect correlations for all analyses. Suggestions are included for researchers and authors to improve the accuracy of data extraction techniques.     

Synthesis and Biological Activity of 10‐Aza‐narciclasine

The first active aza analogue of narciclasine was synthesized from a pentasubstituted derivative of nicotinic acid. The key features of the synthesis include a halogen dance of bromopyridine and an intramolecular Heck reaction with a conduramine derived chemoenzymatically from bromobenzene. 10‐Aza‐narciclasine was found to have reasonable activity against several cancer cell lines.