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排序方式: 共有283条查询结果,搜索用时 0 毫秒
281.
282.
Salber J Gräter S Harwardt M Hofmann M Klee D Dujic J Jinghuan H Ding J Kippenberger S Bernd A Groll J Spatz JP Möller M 《Small (Weinheim an der Bergstrasse, Germany)》2007,3(6):1023-1031
Mechanical stress is a decisive factor for the differentiation, proliferation, and general behavior of cells. However, the specific signaling of mechanotransduction is not fully understood. One basic problem is the clear distinction between the different extracellular matrix (ECM) constituents that participate in cellular adhesion and their corresponding signaling pathways. Here, a system is proposed that enables mechanical stimulation of human-skin-derived keratinocytes and human dermal fibroblasts that specifically interact with peptide sequences immobilized on a non-interacting but deformable substrate. The peptide sequences mimic fibronectin, laminin, and collagen type IV, three major components of the ECM. To achieve this, PDMS is activated using ammonia plasma and coated with star-shaped isocyanate-terminated poly(ethylene glycol)-based prepolymers, which results in a functional coating that prevents unspecific cell adhesion. Specific cell adhesion is achieved by functionalization of the layers with the peptide sequences in different combinations. Moreover, a method that enables the decoration of deformable substrates with cell-adhesion peptides in extremely defined nanostructures is presented. The distance and clustering of cell adhesion molecules below 100 nm has been demonstrated to be of utmost importance for cell adhesion. Thus we present a new toolbox that allows for the detailed analysis of the adhesion of human-skin-derived cells on structurally and biochemically decorated deformable substrates. 相似文献
283.
Martin Reder Jana Holland-Cunz Paula Lorson Anastasia August Britta Nestler 《Advanced Engineering Materials》2023,25(19):2300340
Metal foams constitute a promising and emerging material class in the context of lightweight construction. There exists a variety of different foam topologies, on which resulting mechanical properties depend. To maximize the potential of foams in material use under mechanical load, the present work addresses the question how different geometrical parameters influence the material behaviour. Therefore, an algorithm for digital generation and design of open pore foam structures is presented, that allows to regulate the geometry precisely. A method for retrieving effective mechanical properties from numerical simulations of compression tests in the elastic regime is introduced. Additionally, the representativeness of foam volumes considered for simulations is investigated. This yields a fully digital workflow, which enables the investigation of geometry influence on mechanical properties. This approach is used to conduct simulation studies on generated foam structures with a systematic variation of geometrical parameters. Herein, a range of effective Young's moduli varying by up to a factor of 1.3 for different foam structures at the same porosity is found. This shows a significant impact of the foam geometry on the elastic properties of metal foams. The presented methodology yields insights, which can guide design and optimization of materials for specific applications. 相似文献