Copolymer 1 acts against the immunodominant epitope 82-100 of myelin basic protein by T cell receptor antagonism in addition to major histocompatibility complex blocking

The synthetic random amino acid copolymer Copolymer 1 (Cop 1, Copaxone, glatiramer acetate) suppresses experimental autoimmune encephalomyelitis, slows the progression of disability, and reduces relapse rate in multiple sclerosis (MS). Cop 1 binds to various class II major histocompatibility complex (MHC) molecules and inhibits the T cell responses to several myelin antigens. In this study we attempted to find out whether, in addition to MHC blocking, Cop 1, which is immunologically cross-reactive with myelin basic protein (MBP), inhibits the response to this autoantigen by T cell receptor (TCR) antagonism. Two experimental systems, "prepulse assay" and "split APC assay," were used to discriminate between competition for MHC molecules and TCR antagonism. The results in both systems using T cell lines/clones from mouse and human origin indicated that Cop 1 is a TCR antagonist of the 82-100 epitope of MBP. In contrast to the broad specificity of the MHC blocking induced by Cop 1, its TCR antagonistic activity was restricted to the 82-100 determinant of MBP and could not be demonstrated for proteolipid protein peptide or even for other MBP epitopes. Yet, it was shown for all the MBP 82-100-specific T cell lines/clones tested that were derived from mice as well as from an MS patient. The ability of Cop 1 to act as altered peptide and induce TCR antagonistic effect on the MBP p82-100 immunodominant determinant response elucidates further the mechanism of Cop 1 therapeutic activity in experimental autoimmune encephalomyelitis and MS.     

Design Alternatives of Multithreaded Architecture

This paper compares two possible implementations of multithreaded architecture and proposes a new architecture combining the flexibility of the first with the low hardware complexity of the second. We present performance and step-by-step complexity analysis of two design alternatives of multithreaded architecture: dynamic inter-thread resource scheduling and static resource allocation. We then introduce a new multithreaded architecture based on a new scheduling mechanism called the semi-static. We show that with two concurrent threads the dynamic scheduling processor achieves from 5 to 45 % higher performance at the cost of much more complicated design. This paper indicates that for a relatively high number of execution resources the complexity of the dynamic scheduling logic will inevitably require design compromises. Moreover, high chip-wide communication time and an incomplete bypassing network will limit the dynamic scheduling and reduce its performance advantage. On the other hand, static scheduling architecture achieves low resource utilization. The semi-static architecture utilizes compiler techniques to exploit patterns of program parallelism and introduces a new hardware mechanism, in order to achieve performance close to dynamic scheduling without significantly increasing the static hardware complexity. The semi-static architecture statically assigns part of the functional units but dynamically schedules the most performance-critical functional units on a medium-grain basis.     

Estimation of energy expenditure from heart rate measurements in cattle maintained under different conditions

We examined whether heart rate (HR) could be used to estimate energy expenditure (EE) in cattle. Six Hereford heifers (345 +/- 10.8 kg BW) 12 mo of age were implanted with HR radio transmitters and maintained in individual pens under the following treatments: 1) shade or sun exposure, 2) high- or low-energy diet, and 3) feeding in morning or afternoon. The HR of animals was measured every .5 h during 3 mo; measurements of oxygen consumption and HR were made simultaneously in the morning and in the afternoon while animals were resting and exercising. Average daily HR (52 +/- 4 beats/min) and average daily EE (380 +/- 9 kJ/kg(.75)) in animals on the low-energy diet were less than values in animals on the high-energy diet (94 +/- 4 beats/min and 653 +/- 9 kJ/ kg(.75), respectively). For each animal and within each diet, linear regressions best described the relationship between HR and EE in resting animals, whereas quadratic regressions best described this relationship for exercising animals. The quadratic equation for the exercising animals could also be used for resting animals. In addition, a constant value of EE per heart beat (EE pulse) for each individual resting animal was found and gave accurate estimations. This method was convenient because 1) no exercise equipment was needed to generate the regression equations and 2) EE pulse was less affected by diet than was EE estimated by regression equations. We conclude that HR, a relatively easy measurement, can be useful and accurate in estimating EE. To increase the accuracy of the estimation of EE by HR, the relationship of HR to EE should be established for each animal. In addition, the nutritional regimen for the animal in which EE is estimated should be used for the animal in establishing the relationship.     

Effect of the synchronization of the degradation of dietary crude protein and organic matter and feeding frequency on ruminal fermentation and flow of digesta in the abomasum of dairy cows

Four Holstein cows in midlactation were equipped with ruminal and abomasal cannulas and used to study the effect of synchronized degradation of crude protein (CP) and organic matter (OM) and feeding frequency on digestion and outflow of nutrients. A 4 x 4 Latin square design was used. Diets were arranged in a 2 x 2 factorial design; the four diets contained high ruminally degradable OM and high ruminally degradable CP, high ruminally degradable OM and low ruminally degradable CP, low ruminally degradable OM and high ruminally degradable CP, and low ruminally degradable OM and low ruminally degradable CP. In each period, cows were fed four times daily from d 1 to 14 and two times daily from d 15 to 28. Mean daily ruminal ammonia N concentration was reduced by high ruminally degradable OM, low ruminally degradable CP, and twice daily feeding. Fluctuation in ruminal ammonia N was lower when cows were fed four times daily than when cows were fed twice daily. Plasma urea N concentrations were lower for cows fed diets that were high in ruminally degradable CP. Higher CP flow in the abomasum was found for cows fed the diet containing high ruminally degradable OM and low ruminally degradable CP. Microbial dry matter and CP flow to the abomasum were higher for cows fed twice daily than for cows fed four times daily. Flow of OM in the abomasum was not altered by concentrations of ruminally degradable OM or CP. These results suggest that the available energy in the rumen (ruminally degradable OM) is the most limiting factor for ruminal N utilization under our experimental conditions. Use of these data may improve the prediction of plasma urea N.     

An empirical analysis of the computerization of interactive software evaluation systems

This research studies factor analysis of traditional survey- questionnaires for interactive software evaluation in order to construct a Principal Factor Conversion Function (PFCF). Such a PFCF may be used in several ways: first, for exploratory purposes, to discover the principal factors of the traditional questionnaire; second to test the potential of data reduction within significant loss of information; and third to compare the traditional manual evaluation to a condensed computerized interactive software evaluation. A CAI system was used as the software to be evaluated in this research.     

Ductile and brittle behavior of amorphous polymers. Relationship with activation energy for glass transition and mechanical fracture

An equation correlating the activation energy for the glass transition with T_R, a characteristic reference temperature, the fractional free volume, and the rate of change of the fractional free volume was developed. The resultant activation energies for about 30 polymers are given and favorably compare with the literature. The relationship between the activation energy and the bond-rupture energy indicates whether a polymer will fail in a ductile or brittle fashion. More accurate results are shown to be dependent on the stress, the stress concentration, molecular orientation, frequency of load application, and temperature. Equations correlating all these with the activation energies are given. These results are in agreement with the molecular domain model. Experimental observations from the literature seem to corroborate the suggestion that the molecular domain model holds in the amorphous solid, too.     

Crystallinity of pure and nucleated PET

Pure and Al(OH)₃-containing PET films were prepared, quenched, and subsequently annealed under identical conditions. The level of crystallinity of the films was determined by three methods: density measurements, X-ray crystallinity determination, and measurements by IR of the relative concentration of trans ? O? C? C? O? conformation in the polymer. For pure PET it was found that the percentage crystallinity measured in annealed samples by X-ray and IR is about the same. The density measurements agree with these techniques only when the amorphous density is taken as 1.348 g/cm³. In the case of quenched pure PET, a 7% correction to the concentration of trans conformation must be introduced (in agreement with recent literature) to fit the IR results to the X-ray and density data. Annealed PET containing Al(OH)₃ crystallizes to about the same level as annealed pure PET. The agreement between X-ray and IR data is reasonably good. In the quenched PET/Al(OH)₃ there exists a higher level of trans conformation (enhanced order), probably resulting from adsorption of relatively extended PET chain segments on the surface of the hydroxide particles. These extended units may possibly serve as nucleation sites for PET crystallization upon cooling from the melt.     

Operating systems,compilers, assemblers and application programs: audit trails of user satisfaction

The influence of manufacturer's software on overall computer user satisfaction, as determined by multiple regression, is analysed. Operating systems, number of systems and number of users are found to be the most significant variables affecting overall user satisfaction. The average life of a computer system (in months) and the microcomputer (as the type of installation) have the least significant effect in the user satisfaction equation.An interactive expert system for manufacturer's software is designed, developed and demonstrated. The application of such an expert system is explained along with the use of artificial intelligence techniques. The implications for improvements in manufacturer's software by updates to an artificial-intelligence-based expert system database are discussed and analysed.     

Constructing a Complex Precast Tilt-Up-Panel Structure Utilizing an Optimization Model, 3D CAD, and Animation

This paper presents the concept used to construct a complex residential tilt-up-panel structure utilizing three-dimensional (3D) modeling and animations. The residence comprises of 108 precast concrete panels of varying rectangular shapes with “dog legs” and window and door “cutouts” that look like an assembled jigsaw puzzle. The erection and installation procedure called for a maximum panel-to-panel joint tolerance of 1.27?cm (0.5?in.), often in 90° joints between panels. 3D animations were used to experiment with the construction process on the computer screen prior to construction in order to avoid potential costly on-site errors. In addition, the 3D animations were also used as a training tool for the contractors. This paper focuses on describing the methodology used to integrate a crane selection algorithm and optimization model with 3D modeling and animation for the selection, utilization, and location of cranes on construction sites. Analytical optimization processes were used to decrease the traveling time and distance of the selected crane, to improve the crane lifting sequence and to minimize the use of panel casting slabs.