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91.
92.
Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio
Swapnil Gorakh Waghmare Azin Mohagheghi Samarin Azadeh Mohagheghi Samarin Marianne Danielsen Hanne Sndergrd Mller Tom Policar Otomar Linhart Trine Kastrup Dalsgaard 《International journal of molecular sciences》2021,22(11)
Aging is the most critical factor that influences the quality of post-ovulatory oocytes. Age-related molecular pathways remain poorly understood in fish oocytes. In this study, we examined the effect of oocyte aging on specific histone acetylation in common carp Cyprinus carpio. The capacity to progress to the larval stage in oocytes that were aged for 28 h in vivo and in vitro was evaluated. Global histone modifications and specific histone acetylation (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac) were investigated during oocyte aging. Furthermore, the activity of histone acetyltransferase (HAT) was assessed in fresh and aged oocytes. Global histone modifications did not exhibit significant alterations during 8 h of oocyte aging. Among the selected modifications, H4K12ac increased significantly at 28 h post-stripping (HPS). Although not significantly different, HAT activity exhibited an upward trend during oocyte aging. Results of our current study indicate that aging of common carp oocytes for 12 h results in complete loss of egg viability rates without any consequence in global and specific histone modifications. However, aging oocytes for 28 h led to increased H4K12ac. Thus, histone acetylation modification as a crucial epigenetic mediator may be associated with age-related defects, particularly in oocytes of a more advanced age. 相似文献
93.
Azadeh Ejlali Arash Ejlali Kamel Hooman Hal Gurgenci 《International Communications in Heat and Mass Transfer》2009
A numerical study has been conducted to investigate the fluid flow and heat transfer of an air-cooled metal foam heat exchanger under the high speed laminar jet confined by two parallel walls for which the range of the Reynolds number is 600–1000. Two independent numerical solvers were used and cross-validated being a FORTRAN code and the commercially available software CFD-ACE. The effects of local thermal non-equilibrium, thermal dispersion, porosity, and pore density on the heat transfer augmentation are examined for different Reynolds numbers. Application of energy flux vectors, for convection visualization, is also illustrated for a more comprehensive analysis of the problem. Finally, the performance of the metal foam heat exchanger is compared to that of conventional finned design. It is observed that the heat removal rate can be greatly improved at almost no excess cost. 相似文献
94.
In this study,FLUENT software was employed to simulate the flow pattern and water depth changes in a 120° sharp bend at four discharge rates.To verify the numerical model,a 90° sharp bend was first modeled with a three-dimensional numerical model,and the results were compared with available experimental results.Based on the numerical model validation,a 120° bend was simulated.The results show that the rate of increase of the water depth at the cross-section located 40 cm before the bend,compared with the cross-sections located 40 cm and 80 cm after the bend,decreases with the increase of the normal water depth in the 120° curved channel.Moreover,with increasing normal water depth,the dimensionless water depth change decreases at all cross-sections.At the interior cross-sections of the bend,the transverse water depth slope of the inner half-width is always greater than that of the outer half-width of the channel.Hence,the water depth slope is nonlinear at each crosssection in sharp bends.Two equations reflecting the relationships between the maximum and minimum dimensionless water depths and the normal water depth throughout the channel were obtained. 相似文献
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96.
A. Azadeh M. Hosseinabadi FarahaniS. Torabzadeh M. Baghersad 《Computer methods and programs in biomedicine》2014
This research focuses on scheduling patients in emergency department laboratories according to the priority of patients’ treatments, determined by the triage factor. The objective is to minimize the total waiting time of patients in the emergency department laboratories with emphasis on patients with severe conditions. The problem is formulated as a flexible open shop scheduling problem and a mixed integer linear programming model is proposed. A genetic algorithm (GA) is developed for solving the problem. Then, the response surface methodology is applied for tuning the GA parameters. The algorithm is tested on a set of real data from an emergency department. Simulation results show that the proposed algorithm can significantly improve the efficiency of the emergency department by reducing the total waiting time of prioritized patients. 相似文献
97.
98.
Energy consumption in greenhouses and selection of an optimized heating system with minimum energy consumption 总被引:1,自引:0,他引:1
In this paper, heat loss is precisely computed by a proposed code considering different climates. Estimating the costs of different central heating system, unit heaters were selected as the most feasible system. Finally, considering the heating capacity and unit heater's dimensions a computational fluid dynamics model was developed to find the optimized configuration of unit heaters in a typical greenhouse. Using this model, the required thermal load for a greenhouse based on the daily temperature of Arak city in 2017 was computed with a smart control system. It was found that savings in energy consumption were approximately 5447 m3 of natural gas each year. 相似文献
99.
Buoyant gravity‐based foundations (GBFs) have been proposed as an alternative to conventional lifted GBFs, with the objective to negate the need for costly transportation vessels and thereby provide a more cost‐effective foundation option. However, in order for the foundation to remain stable during float‐out, towing and ballasting, the floatability and hydrodynamic stability of the foundation has to be proven. Maintaining hydrodynamic stability depends on many parameters, most importantly the foundation configuration, geometry and total weight, as well as water depth and ballasting sequence among other factors. This study investigates the performance of GBFs with various geometrical attributes during float‐out and ballasting operations, using a parametric study with water depths ranging from 30 to 50 m. The impact of each parameter on the variation of metacentric height of the foundation during ballasting and the initial drafts at float‐out was studied. The most suitable geometries for each water depth were investigated based on a preliminary analysis of the hydrodynamic stability. One limitation of this work is that the hydrodynamic wave forces acting on the foundation have not been considered—this is the subject of an ongoing experimental programme, which will be published in a follow‐on paper. However, this paper does consider the geotechnical stability of gravity‐based foundations and the potential modes of failure because of soil‐structure interaction. Upper and lower ballast limits were quantified in order to satisfy the geotechnical stability requirements of the foundation for various combinations of water depth/ base diameter. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
100.