基于双CPU技术的泵和马达控制系统的研究与设计

为获取泵和马达的静态与动态特性,设计了基于ADμC812和TMS320F206的双CPU结构的多传感器融合与控制系统。介绍了该系统的体系结构、硬件配置、接口电路、工作原理和工作流程。实现了传感器信息融合的高速高精度采集、复杂算法的大数据量实时计算以及模拟与开关量并有的多通道控制等功能,系统硬件结构简单。该系统作为泵、马达综合试验台的子系统,用于泵、马达性能试验。应用表明该系统能够满足试验要求,具有很好的可靠性和实时性。     

一种精确采集汽轮机蒸汽压力的实现方法

针对共振机理低频振荡问题,考虑汽轮机热力系统和电力系统的相互影响,对汽轮机蒸汽压力的精确采集提出了一种实现方法。该研究成果对电力系统减小低频振荡具有一定的经济价值。     

中风化花岗岩中嵌岩桩承载性能原位试验与极限承载力预测

为深入探究中风化花岗岩中嵌岩桩的竖向抗压承载特性,对12根嵌岩桩进行了单桩竖向抗压静载原位试验与ABAQUS有限元数值模拟,通过多种方法对嵌岩单桩极限承载力进行评价,明确中风化花岗岩中嵌岩桩竖向抗压承载性状。研究表明:12根中风化花岗岩中嵌岩桩并非表现出完全端承桩,而是呈摩擦型桩或摩擦端承桩的性状;中风化花岗岩地基中的嵌岩桩竖向抗压极限承载力较高,桩顶沉降小,满足工程对基础的承载要求;有限元模拟荷载-沉降曲线与实测荷载-沉降曲线走势吻合度较高,桩顶沉降误差较小;本试验条件下,桩端阻力占桩顶荷载的56.9%,桩侧摩阻力占比为43.1%,桩侧摩阻力在荷载传递过程中发挥较充分;有限元模拟得到的单桩极限承载力与指数函数模型的预测结果较为吻合,可用于嵌岩桩单桩竖向抗压极限承载力的预测,以及嵌岩桩承载性状和荷载传递规律的分析。     

基于有限状态机的电网多源时空数据的区块链追溯方法研究

电网基础数据类型多、变化关系复杂,地理空间、时间周期跨度大,数据追溯困难,该文提出基于区块链技术的电网多源数据追溯方法。根据电网基础数据时空分布及数据流变动性特点,建立了基于区块链的多源数据追溯架构,研究了数据追溯依赖关系及溯源路径结构。提出了基于有限状态机模型的数据溯源的路径结构分析图,及路径构建算法,研究了多源时空数据区块的平行追溯方式。构建了基于有限状态机溯源路径的电网多源时空数据追溯优化流程,研究了多层次溯源数据区块时空序列关联性。工程实例表明,基于区块链、有限状态机可高效灵活追溯电网基础多源时空数据的完整可信分布序列。     

Study on destructions and reinforcement measures of mined constructions

Ground movement and deformation caused by underground mining would destroy the constructions located within subsidence area. Constructions would suffer different destructions with different location. The effect of ground deformation caused by underground mining to constructions was analyzed. Analyzed the destructive types of constructions in different ground movement and deformation. Taking a two-storey building for example, the walls were reinforced by computation before mining. To protect the construction the reinforcement measures to the construction before mining were studied.     

Generation and Identification of the Number of Copies of Exogenous Genes and the T-DNA Insertion Site in SCN-Resistance Transformation Event ZHs1-2

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) causes an estimated economic loss of about USD 3 billion each year in soybean (Glycine max L.) production worldwide. Overexpression of resistance genes against SCN provides a powerful approach to develop SCN resistance cultivars in soybean. The clarification of molecular characterization in transformation events is a prerequisite for ecological risk assessment, food safety, and commercial release of genetically modified crops. Here, we generated transgenic events harboring the BCN (beet cyst nematode) resistance Hs1^pro−1 gene using the Agrobacterium-mediated method in soybean, evaluated their resistance to SCN infection, and clarified the molecular characterization of one of the transformation events. Five independent and stable inheritable transformation events were generated by an Agrobacterium-mediated transformation method. SCN resistance tests showed the average number of developed females per plant and female index (FI) in T4 ZHs1-1, ZHs1-2, ZHs1-3, ZHs1-4, and ZHs1-5 transformation events were significantly lower than that in the nontransgenic control. Among these, the ZHs1-2 transformation event had the lowest number of developed females per plant and FI. Southern hybridization showed the exogenous target Hs1^pro−1 gene was inserted in one copy and the Bar gene was inserted two copies in the ZHs1-2 transformation event. The exogenous T-DNA fragment was integrated in the reverse position of Chr02: 5351566–5231578 (mainly the Bar gene expression cassette) and in the forward position of Chr03: 17083358–17083400 (intact T-DNA, including Hs1^pro−1 and Bar gene expression cassette) using a whole genome sequencing method (WGS). The results of WGS method and Southern hybridization were consistent. All the functional elements of exogenous T-DNA fragments were verified by PCR using specific primer pairs in the T5 and T6 ZHs1-2 transformation events. These results demonstrated that the overexpression of Hs1^pro−1 gene enhanced SCN resistance, and provide an important reference for the biosafety assessment and the labeling detection in transformation event ZHs1-2.     

关井阶段CO2作储气库垫层气的动态影响规律

目的为应对地下储气库中的垫层气损失,采用经济气体CO₂作为垫层气,目前,CO₂作垫层气在实际应用中存在各种条件限制,且对其在关井阶段的应用研究较少,有必要对在关井阶段CO₂作垫层气对天然气储气库的运行影响因素进行研究。 方法利用有限元模拟CO₂作储气库垫层气时,研究关井阶段储气库动态参数(注气压力、注气速率和CO₂垫层气比例)对混气带的影响规律。 结果注气压力对混气带的影响不大,将其控制在12 MPa左右最为合理,此时混气带占储气面积的比例为23.731 5%;混气带面积占比随注气速率的增大而减小,但是在注气口附近会出现混合区域,导致回采天然气时出现大量的混合气体,所以注气速率控制在0.7×10⁸ m³/d时最为合理,这时混气带面积占比为18.324 6%;CO₂作垫层气的比例对天然气-CO₂之间的混合影响明显,当CO₂垫层气比例为20%时,混气带面积占比为7.236 5%。 结论根据实验结果设计针对混气带的控制措施,当注气压力控制为12 MPa时,注气速率为0.7×10⁸ m³/d,当CO₂垫层气比例为20%时,能让储气库的运作更为经济,实验结果可为实际储气库的建设提供参考。      

Development of a Multi-Epitope Vaccine for Mycoplasma hyopneumoniae and Evaluation of Its Immune Responses in Mice and Piglets

Mycoplasma hyopneumoniae (Mhp), the primary pathogen causing Mycoplasma pneumonia of swine (MPS), brings massive economic losses worldwide. Genomic variability and post-translational protein modification can enhance the immune evasion of Mhp, which makes MPS prone to recurrent outbreaks on farms, even with vaccination or other treatments. The reverse vaccinology pipeline has been developed as an attractive potential method for vaccine development due to its high efficiency and applicability. In this study, a multi-epitope vaccine for Mhp was developed, and its immune responses were evaluated in mice and piglets. Genomic core proteins of Mhp were retrieved through pan-genome analysis, and four immunodominant antigens were screened by host homologous protein removal, membrane protein screening, and virulence factor identification. One immunodominant antigen, {"type":"entrez-protein","attrs":{"text":"AAV27984.1","term_id":"53987783","term_text":"AAV27984.1"}}AAV27984.1 (membrane nuclease), was expressed by E. coli and named rMhp597. For epitope prioritization, 35 B-cell-derived epitopes were identified from the four immunodominant antigens, and 10 MHC-I and 6 MHC-II binding epitopes were further identified. The MHC-I/II binding epitopes were merged and combined to produce recombinant proteins MhpMEV and MhpMEVC6His, which were used for animal immunization and structural analysis, respectively. Immunization of mice and piglets demonstrated that MhpMEV could induce humoral and cellular immune responses. The mouse serum antibodies could detect all 11 synthetic epitopes, and the piglet antiserum suppressed the nuclease activity of rMhp597. Moreover, piglet serum antibodies could also detect cultured Mhp strain 168. In summary, this study provides immunoassay results for a multi-epitope vaccine derived from the reverse vaccinology pipeline, and offers an alternative vaccine for MPS.     

Spatial distribution of laser energy and its influence on the stability of extreme narrow keyholes during ultra-high power laser welding

The instability of extremely narrow keyholes is the main challenge in high-power laser welding.In this work,the spatial energy distribution and its influence on the stability of extremely narrow keyholes during ultra-high power laser welding are studied.A multiphase flow model that considers the vapor plume impact and the multiple scattering of laser radiation is built to simulate the topology and downstream wave flow on the keyhole wall.Drastic keyhole fluctuation is due to excessive energy acc...     

亭台楼阁类建筑的文学价值

亭台楼阁作为中国古代的传统建筑,其特殊的体式、功能及其与自然环境所构成的独特意境,成为历代文人登高临远的重要场所,形成了大量诗文,这些作品往往寄寓了游子怀乡、壮士悲歌、文士不遇等情感,许多名篇对后人产生了强大的精神感召力,成为中华民族精神文化遗产的一部分。