What is the role of fresh groundwater and recirculated seawater in conveying nutrients to the coastal ocean?

Submarine groundwater discharge (SGD) is a major process operating at the land-sea interface. Quantifying the SGD nutrient loads and the marine/terrestrial controls of this transport is of high importance, especially in oligotrophic seas such as the eastern Mediterranean. The fluxes of nutrients in groundwater discharging from the seafloor at Dor Bay (southeastern Mediterranean) were studied in detail using seepage meters. Our main finding is that the terrestrial, fresh groundwater is the main conveyor of DIN and silica to the coastal water, with loads of 500 and 560 mol/yr, respectively, per 1 m shoreline. Conversely, recirculated seawater is nutrient-poor, and its role is mainly as a dilution agent. The nutrient loads regenerated in the subterranean estuary (sub-bay sediment) are relatively small, consisting mostly of ammonium (24 mol/yr). On the other hand, the subterranean estuary at Dor Bay sequesters as much as 100 mol N/yr per 1 m shoreline, mainly via denitrification processes. These, and observations from other SGD sites, imply that the subterranean estuary at some coastal systems may function more as a sink for nitrogen than a source. This further questions the extent of nutrient contributions to the coastal water by some subterranean estuaries and warrants systematic evaluation of this process in various hydrological and marine trophic conditions.     

Residual porosity and optical properties of spark plasma sintered transparent polycrystalline cerium-doped YAG

Transparent cerium-doped yttrium aluminum garnet (Ce:YAG) phosphors are promising candidates for high-power white light emitting diode applications. In the present study, Ce:YAG powder was synthesized by a co-precipitation method and highly transparent ceramics were fabricated by spark plasma sintering. The effects of temperature and pressure, as well as post-sintering treatments (annealing or hot isostatic pressing), on residual porosity were studied by electron and confocal laser microscopy. Correlation between residual porosity characteristics (pore size and volume fraction) and optical properties (in-line transmittance and photoluminescence intensity) of the luminescent transparent ceramics was established.     

Secure Computation Without Authentication

Research on secure multiparty computation has mainly concentrated on the case where the parties can authenticate each other and the communication between them. This work addresses the question of what security can be guaranteed when authentication is not available. We consider a completely unauthenticated setting, where all messages sent by the parties may be tampered with and modified by the adversary without the uncorrupted parties being able to detect this fact. In this model, it is not possible to achieve the same level of security as in the authenticated-channel setting. Nevertheless, we show that meaningful security guarantees can be provided: Essentially, all the adversary can do is to partition the network into disjoint sets, where in each set the computation is secure in of itself, and also independent of the computation in the other sets. In this setting we provide, for the first time, nontrivial security guarantees in a model with no setup assumptions whatsoever. We also obtain similar results while guaranteeing universal composability, in some variants of the common reference string model. Finally, our protocols can be used to provide conceptually simple and unified solutions to a number of problems that were studied separately in the past, including password-based authenticated key exchange and nonmalleable commitments. As an application of our results, we study the question of constructing secure protocols in partially authenticated networks, where some of the links are authenticated, and some are not (as is the case in most networks today).     

Resilient gossip algorithms for collecting online management information in exascale clusters

Management of forthcoming exascale clusters requires frequent collection of run‐time information about the nodes and the running applications. This paper presents a new paradigm for providing online information to the management system of scalable clusters, consisting of a large number of nodes and one or more masters that manage these nodes. We describe the details of resilient gossip algorithms for sharing local information within subsets of nodes and for sending global information to a master, which holds information on all the nodes. The presented algorithms are decentralized, scalable and resilient, working well even when some nodes fail, without needing any recovery protocol. The paper gives formal expressions for approximating the average ages of the local information at each node and the information collected by the master. It then shows that these results closely match the results of simulations and measurements on a real cluster. The paper also investigates the resilience of the algorithms and the impact on the average age when nodes or masters fail. The main outcome of this paper is that partitioning of large clusters can improve the quality of information available to the management system without increasing the number of messages per node. Copyright © 2015 John Wiley & Sons, Ltd.     

Anti-mutagenic activity of DNA damage-binding proteins mediated by direct inhibition of translesion replication

DNA lesions that block replication can be bypassed in Escherichia coli by a special DNA synthesis process termed translesion replication. This process is mutagenic due to the miscoding nature of the DNA lesions. We report that the repair enzyme formamido-pyrimidine DNA glycosylase and the general DNA damage recognition protein UvrA each inhibit specifically translesion replication through an abasic site analog by purified DNA polymerases I and II, and DNA polymerase III (alpha subunit) from E. coli. In vivo experiments suggest that a similar inhibitory mechanism prevents at least 70% of the mutations caused by ultraviolet light DNA lesions in E. coli. These results suggest that DNA damage-binding proteins regulate mutagenesis by a novel mechanism that involves direct inhibition of translesion replication. This mechanism provides anti-mutagenic defense against DNA lesions that have escaped DNA repair.     

Bounds on achievable rates of LDPC codes used over the binary erasure channel

We derive upper bounds on the maximum achievable rate of low-density parity-check (LDPC) codes used over the binary erasure channel (BEC) under Gallager's decoding algorithm, given their right-degree distribution. We demonstrate the bounds on the ensemble of right-regular LDPC codes and compare them with an explicit left-degree distribution constructed from the given right degree.     

Germination, growth, and sporulation of Bacillus thuringiensis subsp. israelensis in excreted food vacuoles of the protozoan Tetrahymena pyriformis

Spores of Bacillus thuringiensis subsp. israelensis and their toxic crystals are bioencapsulated in the protozoan Tetrahymena pyriformis, in which the toxin remains stable. Each T. pyriformis cell concentrates the spores and crystals in its food vacuoles, thus delivering them to mosquito larvae, which rapidly die. Vacuoles containing undigested material are later excreted from the cells. The fate of spores and toxin inside the food vacuoles was determined at various times after excretion by phase-contrast and electron microscopy as well as by viable-cell counting. Excreted food vacuoles gradually aggregated, and vegetative growth of B. thuringiensis subsp. israelensis was observed after 7 h as filaments that stemmed from the aggregates. The outgrown cells sporulated between 27 and 42 h. The spore multiplication values in this system are low compared to those obtained in carcasses of B. thuringiensis subsp. israelensis-killed larvae and pupae, but this bioencapsulation represents a new possible mode of B. thuringiensis subsp. israelensis recycling in nontarget organisms.     

Lipoamide dehydrogenase deficiency: a newly discovered cause of acute hepatitis in adults

Lipoamide dehydrogenase deficiency is a rare disease, manifested in early childhood by lactic acidemia, progressive neurological damage and death in most cases. We report a case of lipoamide dehydrogenase deficiency in a 34-year-old Ashkenazi-Jewish woman. The deficiency manifested as acute hepatitis without cognitive impairment or acidosis. The patient's brother also had lipoamide dehydrogenase deficiency, diagnosed at the age of 20, and manifested as hepatocellular damage, lactic acidemia and myoglobinuria. We assume that the trigger for this hepatocellular damage was prolonged fasting, and that otherwise the patient might have gone undiagnosed. Other cases in Ashkenazi Jews of mild lipoamide dehydrogenase deficiency with hepatocellular injury but without central nervous system involvement are reviewed.     

The G protein-coupled receptor kinase 2 is a microtubule-associated protein kinase that phosphorylates tubulin

The G protein-coupled receptor kinase 2 (GRK2) is a serine/threonine kinase that phosphorylates and desensitizes agonist-occupied G protein-coupled receptors (GPCRs). Here we demonstrate that GRK2 is a microtubule-associated protein and identify tubulin as a novel GRK2 substrate. GRK2 is associated with microtubules purified from bovine brain, forms a complex with tubulin in cell extracts, and colocalizes with tubulin in living cells. Furthermore, an endogenous tubulin kinase activity that copurifies with microtubules has properties similar to GRK2 and is inhibited by anti-GRK2 monoclonal antibodies. Indeed, GRK2 phosphorylates tubulin in vitro with kinetic parameters very similar to those for phosphorylation of the agonist-occupied beta2-adrenergic receptor, suggesting a functionally relevant role for this phosphorylation event. In a cellular environment, agonist occupancy of GPCRs, which leads to recruitment of GRK2 to the plasma membrane and its subsequent activation, promotes GRK2-tubulin complex formation and tubulin phosphorylation. These findings suggest a novel role for GRK2 as a GPCR signal transducer mediating the effects of GPCR activation on the cytoskeleton.