Effect of triterpene glycosides on RNA biosynthesis in a yeast cell culture of Saccharomyces carlsbergensis

The effect of triterpen glycosides of cauloside C from Caulophyllum robustum, theasaponine from Thea sinensis ahd stichoposide A from Stichopus japonicus on multiplication and biosynthesis of RNA in the cells of a 7-hour culture of Saccharomyces carlsbergensis was studied. It was shown that cauloside C, theasaponine and stichoposide A in concentrations of 7.5 gamma/ml inhibited multiplication of the yeast cells by 65, 10 and 90 per cent respectively. The summation RNA of the yeast cells is divided into 3 zones on Sephadex G-100. The glycosides induced no pronounced changes in the chromotographic profile of RNA. Biosynthesis of the transport and ribosomal RNA were inhibited to the same extent. Triterpen glycosides inhibited the biosynthesis of RNA at the stage of 14C-uridine in corporation into the nucleotide pool of the yeast cells.     

Detection of trace quantities of10B in biosamples using track detectors

A new variant of a solid-state detector adapted for estimating the¹⁰B content in biological tissue was developed. A new polymeric composition based on oligoester acrylates was used as the detector. The number of tracks is a linear function of the boron content in the concentration range 0.5–200 μg¹⁰B/g inbiological sample samples with mass 100–500 μg. The detector can be recommended for deterning¹⁰B in biological tissues, mineralogy, and agrochemistry, 3 figures, 2 references. Translated from Atomnaya énergiya, Vol. 88, No. 3, pp. 194–197, March, 2000.