A headspace solid-phase microextraction method development and its application in the determination of volatiles in honeys by gas chromatography

The first part of the paper presents the detailed optimisation of all steps of the determination procedure of volatiles from Polish honeys by headspace solid-phase microextraction (HS-SPME) as the sample preparation method and gas chromatography/mass spectrometry (GC–MS) as the method of final determination. In the result, the following conditions were applied: CAR/PDMS/DVB SPME fibre, the sample composition (5:1 w/w honey to water ratio), 3 g sample size. The other procedure parameters, like the temperatures of individual stages of the process, were also included.     

Simplified transient space-charge-limited current measurements of mobility using transimpedance amplifier

Transient space-charge-limited current measurement (also called time resolved dark injection) is a well known technique for mobility and contact ohmicity characterization in organic thin films. We demonstrate the feasibility of performing space-charge-limited transient current measurements using a transimpedance amplifier instead of the typically used bridge circuit. The use of a transimpedance amplifier significantly simplifies the experiment, particularly because it removes the requirements for bridge symmetry and per sample adjustment. Furthermore the experimental bandwidth and current sensitivity are increased. We detail the experiment design and verify the compatibility of measurements on a [6,6]-phenyl-C60-butyric acid methyl ester (60PCBM) blend thin film.     

Pharmacogenetics of Drug-Resistant Epilepsy (Review of Literature)

Pharmacogenomic studies in epilepsy are justified by the high prevalence rate of this disease and the high cost of its treatment, frequent drug resistance, different response to the drug, the possibility of using reliable methods to assess the control of seizures and side effects of antiepileptic drugs. Candidate genes encode proteins involved in pharmacokinetic processes (drug transporters, metabolizing enzymes), pharmacodynamic processes (receptors, ion channels, enzymes, regulatory proteins, secondary messengers) and drug hypersensitivity (immune factors). This article provides an overview of the literature on the influence of genetic factors on treatment in epilepsy.     

Measurement of nuclear DNA content in fission yeast by flow cytometry

Cell division cycle (cdc) mutants of Schizosaccharomyces pombe are arrested at specific points in the cell cycle when grown at restrictive temperature. Flow cytometry of such cells reveals an anomalous increase in the DNA fluorescence signal, which represents a problem in experiments designed to determine the cell cycle arrest point. The increased fluorescence signal is due to cytoplasmic constituents and has been attributed to mitochondrial DNA synthesis (S. Sazer and S. W. Sherwood, J. Cell Sci.97: 509–516, 1990). Here we have studied the cdc10 mutant by flow cytometry using different DNA-binding fluorochromes and found no evidence that the increased fluorescence signal was caused by mitochondrial DNA synthesis. To determine more accurately the nuclear DNA content we have developed a novel method to remove most of the cytoplasmic material by exposing the cells to Triton X-100 and hypotonic conditions after cell wall digestion. The DNA fluorescence from cells treated in this way was more constant with time of incubation at restrictive temperature in spite of a considerable increase in cell size. With this method we could determine that the recently isolated temperature sensitive orp1 mutant is arrested with a 1C DNA content. Premature and abnormal mitosis (‘cut’) could be observed for the orp1 mutant after only 4 h at restrictive temperature. © 1997 John Wiley & Sons, Ltd.     

Secretome of Adipose Tissue as the Key to Understanding the Endocrine Function of Adipose Tissue

The prevalence of obesity has reached pandemic levels and is becoming a serious health problem in developed and developing countries. Obesity is associated with an increased prevalence of comorbidities that include type II diabetes, cardiovascular diseases and some cancers. The recognition of adipose tissue as an endocrine organ capable of secreting adipokines that influence whole-body energy homeostasis was a breakthrough leading to a better molecular understanding of obesity. Of the adipokines known to be involved in the regulation of energy metabolism, very few are considered central regulators of insulin sensitivity, metabolism and energy homeostasis, and the discovery and characterization of new adipocyte-derived factors are still ongoing. Proteomics techniques, such as liquid chromatography-mass spectrometry or gas chromatography-mass spectrometry, have proven to be useful tools for analyzing the secretory function of adipose tissue (the secretome), providing insights into molecular events that influence body weight. Apart from the identification of novel proteins, the considerable advantage of this approach is the ability to detect post-translational modifications that cannot be predicted in genomic studies. In this review, we summarize recent efforts to identify novel bioactive secretory factors through proteomics.     

The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes

In view of the possible medical applications of saponins, the molecular structure of a GOTCAB saponin from the roots of Gypsophila paniculata L. was determined by NMR. The biological activity of saponins may depend on the interaction with cell membranes. To obtain more insight in the mechanism of membrane-related saponin function, an experimental and theoretical study was conducted. Ternary lipid systems composed of sphingomyelin, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, and cholesterol were used as models of mammalian cell membranes. The membrane–saponin interaction was studied experimentally by monitoring surface pressure in the monomolecular films formed at the air–aqueous subphase interface. The behavior of GOTCAB saponin in a water box and model monolayer systems was characterized by molecular dynamics simulations. The results obtained showed that, in the systems used, cholesterol had a decisive effect on the interaction between GOTCAB and phosphocholine or sphingomyelin as well as on its location within the lipid film.     

Carbon black modified with 4-hydroxymethylbenzenediazonium salt as filler for phenol-formaldehyde resins and abrasive tools

Although phenol-formaldehyde (PF) resins possess excellent properties, they emit toxic substances during the decomposition of the crosslinking agent. Therefore, studies are being carried out on the fillers, which can reduce the amount of crosslinking agent. In this article, the synthesis of diazonium-modified carbon black was conducted. Diazonium modification allows the effective incorporation of hydroxymethyl groups, which are responsible for the crosslinking of PF resins. Modified materials were used as active fillers in the PF composites and abrasive tools. Due to carbon modification, flexural strength increases 26% for PF resin composite and 1100% for abrasive tools. The increase is related to the resulting covalent bonds between the resin and the modified filler. Therefore, it can be stated that application of active filler improves the properties of novolac composites and abrasive tools. The use of this type of fillers may affect the reduction of the amount of crosslinking agent in the future. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137, 48160.     

Recent Advances in Metallic Nanoparticle Assemblies for Surface-Enhanced Spectroscopy

Robust and versatile strategies for the development of functional nanostructured materials often focus on assemblies of metallic nanoparticles. Research interest in such assemblies arises due to their potential applications in the fields of photonics and sensing. Metallic nanoparticles have received considerable recent attention due to their connection to the widely studied phenomenon of localized surface plasmon resonance. For instance, plasmonic hot spots can be observed within their assemblies. A useful form of spectroscopy is based on surface-enhanced Raman scattering (SERS). This phenomenon is a commonly used in sensing techniques, and it works using the principle that scattered inelastic light can be greatly enhanced at a surface. However, further research is required to enable improvements to the SERS techniques. For example, one question that remains open is how to design uniform, highly reproducible, and efficiently enhancing substrates of metallic nanoparticles with high structural precision. In this review, a general overview on nanoparticle functionalization and the impact on nanoparticle assembly is provided, alongside an examination of their applications in surface-enhanced Raman spectroscopy.     

Development of an Autochthonous Microbial Consortium for Enhanced Bioremediation of PAH-Contaminated Soil

The main objectives of this study were to isolate bacteria from soil chronically contaminated with polycyclic aromatic hydrocarbons (PAHs), develop an autochthonous microbial consortium, and evaluate its ability to degrade PAHs in their native contaminated soil. Strains with the best bioremediation potential were selected during the multi-stage isolation process. Moreover, to choose bacteria with the highest bioremediation potential, the presence of PAH-degrading genes (pahE) was confirmed and the following tests were performed: tolerance to heavy metals, antagonistic behavior, phytotoxicity, and antimicrobial susceptibility. In vitro degradation of hydrocarbons led to the reduction of the total PAH content by 93.5% after the first day of incubation and by 99.22% after the eighth day. Bioremediation experiment conducted in situ in the contaminated area resulted in the average reduction of the total PAH concentration by 33.3% after 5 months and by over 72% after 13 months, compared to the concentration recorded before the intervention. Therefore, this study implicates that the development of an autochthonous microbial consortium isolated from long-term PAH-contaminated soil has the potential to enhance the bioremediation process.