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1.
A novel assay based on a nested PCR and restriction enzyme analysis of the PCR products was developed for the rapid detection and identification of Mycobacterium bovis and M. avium-M. intracellulare species in formalin-fixed, paraffin-embedded tissue (PET) specimens. On the basis of the nucleotide sequence data obtained in the present study, general nested primers were constructed to amplify a 424-bp segment of the gene encoding the 65-kDa surface antigen of mycobacteria. The nested PCR assay proved to be highly sensitive, since as little as 5 to 10 fg of extracted mycobacterial DNA was detected. The safety of the assay as a routine method for the diagnosis of M. bovis and M. avium-M. intracellulare in PET specimens was provided by taking various precautions. In order to prevent false positivity, specific tools and procedures were applied. To detect false-negative results and assess the efficiency of the PCR, an internal standard molecule of amplification was constructed. The digestion of the amplicons with the restriction endonuclease Sau96-I allowed the identification of M. bovis and M. avium-M. intracellulare in a large number of clinical specimens. The present results indicate that PCR combined with an internal control of amplification and restriction enzyme analysis of the amplicons provides a rapid, sensitive, and reliable method for routine diagnostic laboratories to detect and identify M. bovis and M. avium-M. intracellulare in PET specimens. 相似文献
2.
3.
A nonlinear model for holographic recording materials is used to evaluate the signal-to-noise ratio in diffuse-object holograms. A comparison of this model with the linear model proposed by Upatnieks and Leonard [J. Opt. Soc. Am. 60, 297 (1970)] shows that our model justifies the experimental results obtained for dieletric holograms at high density before bleaching. 相似文献
4.
Collision avoidance is essential for safe robot manipulation. Especially with humans around, robots should work only when safety can be robustly guaranteed. In this paper, we propose using virtual impedance control for reactive, smooth, and consistent collision avoidance that interferes minimally with the original task. The virtual impedance control operates in the risk space, a vector space describing the possibilities of all forthcoming collisions, and is designed to elude all risks in a consistent response in order to create assuring human-robot interaction experiences. The proposed scheme intrinsically handles kinematic singularity and the activation of avoidance using a boundary layer defined on the spectrum of Jacobian. In cooperation with the original controller, the proposed avoidance scheme provides a proof of convergence if the original controller is stable with and without projection. In simulations and experiments, we verified the characteristics of the proposed control scheme and integrated the system with Microsoft Kinect to monitor the workspace for real-time collision detection and avoidance. The results show that the proposed approach is suitable for robot operation with humans nearby. 相似文献
5.
Vallati Carlo Mingozzi Enzo Tanganelli Giacomo Buonaccorsi Novella Valdambrini Nicola Zonidis Nikolaos Martínez Belén Mamelli Alessandro Sommacampagna Davide Anggorojati Bayu Kyriazakos Sofoklis Prasad Neeli Nieto Francisco Javier Rodriguez Oliver Barreto 《Wireless Personal Communications》2016,87(3):1071-1091
Wireless Personal Communications - The integration of everyday objects into the Internet represents the foundation of the forthcoming Internet of Things (IoT). Smart objects will be the building... 相似文献
6.
Rafael Asorey-Cacheda Belén Pedrero-López Francisco J. González-Castaño 《Multimedia Tools and Applications》2013,64(3):627-647
Implicit Error Correction is a near Video-on-Demand (nVoD) schema that trades bandwidth utilization for initial playback delay while potentially allowing an infinite number of users. Additionally, it provides error protection without further bandwidth increase by exploiting the implicit redundancy of nVoD protocols, using linear combinations of the segments transmitted in a given time slot. This paper introduces a new implementation that outperforms the original schema by adding two new mechanisms: redundancy channels and feedback error correction. 相似文献
7.
Jéssica Gil-Serna Covadonga Vázquez Noelia Sardiñas Ma Teresa González-Jaén Belén Patiño 《Food Control》2011,22(2):343-345
Ochratoxin A (OTA) is a highly toxigenic mycotoxin commonly present in a number of diverse agroproducts. Aspergillus Section Circumdati includes some of the most important OTA-producing species: Aspergillus ochraceus, Aspergillus elegans and the recently described Aspergillus steynii and Aspergillus westerdijkiae. In this work, OTA production by several strains of these four species from diverse origins and food matrices was examined. Identification of all the strains was carried out by specific PCR assays. The strains were cultivated in CYA solid medium and OTA was measured by HLPC. This study demonstrated for the first time the high capacity of A. steynii strains to produce OTA at higher levels than A. westerdijkiae,A. ochraceus and A. elegans strains showed low levels or no production at all. In conclusion, the results of this study suggest that the occurrence of A. steynii and A. westerdijkiae might represent the major potential risk for OTA contamination due to their high production and the diversity of commodities that these species may contaminate. 相似文献
8.
González-Fonteboa Belén Martínez-Abella Fernando Carro López Diego Seara-Paz Sindy 《Construction and Building Materials》2011,25(5):2335-2342
In this work the stress–strain curve of the recycled concretes was determined by replacing different percentages of the natural coarse aggregate with recycled coarse aggregate (20%, 50% and 100%). The results made it possible to establish the differences between the stress–strain relationship of a conventional concrete and this relationship for a recycled concrete depending on the percentage of replacement. In this way, it was found that the longitudinal strain of the recycled concretes increases with the percentage of recycled coarse aggregate used.Finally, using the experimental results, an analytical expression of the stress–strain curve accounting for the percentage of replacement was developed. The verification of the proposed model equation was done by comparing it to the experimental data. The results show that the proposed model equation satisfactorily describes the effect of the recycled aggregate on the stress–strain curve. 相似文献
9.
Polysaccharides,oligosaccharides and nitrogenous compounds change during the ageing of Tempranillo and Verdejo sparkling wines 下载免费PDF全文
10.
Sardiñas N Vázquez C Gil-Serna J González-Jaén MT Patiño B 《International journal of food microbiology》2011,145(1):121-125
Aflatoxins are important mycotoxins that represent a serious risk for human and animal health. These mycotoxins are mainly produced by Aspergillus flavus and Aspergillus parasiticus, two closely related species with different array of aflatoxins. In this work, two specific quantitative PCR (qPCR) assays were developed to detect and quantify both species in wheat flour using primers based on the multicopy ITS2 rDNA target sequence. The species specificity of the assays was tested in a wide range of strains of these species and others colonizing the same commodities. The sensitivity of the assay was estimated in 2.5 pg/reaction in both species. Discrimination capacity for detection and relative quantification of A. flavus and A. parasiticus DNA were analyzed using samples with DNA mixtures containing also other fungal species at different ratios. Both qPCR assays could detect spore concentrations equal or higher than 106 spores/g in flour samples without prior incubation. These assays are valuable tools to improve diagnosis at an early stage and in all critical control points of food chain integrated in HACCP strategies. 相似文献