Enzymatic Addition of Alcohols to Terpenes by Squalene Hopene Cyclase Variants

Squalene–hopene cyclases (SHCs) catalyze the polycyclization of squalene into a mixture of hopene and hopanol. Recently, amino-acid residues lining the catalytic cavity of the SHC from Alicyclobacillus acidocaldarius were replaced by small and large hydrophobic amino acids. The alteration of leucine 607 to phenylalanine resulted in increased enzymatic activity towards the formation of an intermolecular farnesyl–farnesyl ether product from farnesol. Furthermore, the addition of small-chain alcohols acting as nucleophiles led to the formation of non-natural ether-linked terpenoids and, thus, to significant alteration of the product pattern relative to that obtained with the wild type. It is proposed that the mutation of leucine at position 607 may facilitate premature quenching of the intermediate by small alcohol nucleophiles. This mutagenesis-based study opens the field for further intermolecular bond-forming reactions and the generation of non-natural products.     

Anonymous attestation with user-controlled linkability

This paper is motivated by the observation that existing security models for direct anonymous attestation (DAA) have problems to the extent that insecure protocols may be deemed secure when analysed under these models. This is particularly disturbing as DAA is one of the few complex cryptographic protocols resulting from recent theoretical advances actually deployed in real life. Moreover, standardization bodies are currently looking into designing the next generation of such protocols. Our first contribution is to identify issues in existing models for DAA and explain how these errors allow for proving security of insecure protocols. These issues are exhibited in all deployed and proposed DAA protocols (although they can often be easily fixed). Our second contribution is a new security model for a class of “pre-DAA scheme”, that is, DAA schemes where the computation on the user side takes place entirely on the trusted platform. Our model captures more accurately than any previous model the security properties demanded from DAA by the trusted computing group (TCG), the group that maintains the DAA standard. Extending the model from pre-DAA to full DAA is only a matter of refining the trust models on the parties involved. Finally, we present a generic construction of a DAA protocol from new building blocks tailored for anonymous attestation. Some of them are new variations on established ideas and may be of independent interest. We give instantiations for these building blocks that yield a DAA scheme more efficient than the one currently deployed, and as efficient as the one about to be standardized by the TCG which has no valid security proof.     

A socially aware caching mechanism for encounter networks

Managing community content in mobile communities is challenging, since informal groups may emerge spontaneously whenever opportunities exist. To deal with group dynamics, encounter based community support systems can be a solution. In encounter networks, peers exchange content whenever they physically meet, i.e., whenever peers are near to each other. To assure content availability within the community network, content needs to be replicated (cached) and continuously updated. A?well known drawback of this scheme is the fact that the resource usage can be quite high. In this paper we present new caching strategies based on sociological knowledge, aiming at improving the overall content quality. We derive mobility and connectivity aware cache policies and simulate their benefit for knowledge building with urban life simulations. Results show that context precision and recall can be greatly improved in comparison to traditional cache strategies applied in the majority of encounter networks.     

Privacy protection vs. utility in visual data

Multimedia Tools and Applications - Ubiquitous and networked sensors impose a huge challenge for privacy protection which has become an emerging problem of modern society. Protecting the privacy of...     

[2.2]Paracyclophane-Based TCN-201 Analogs as GluN2A-Selective NMDA Receptor Antagonists

Recent studies have shown the involvement of GluN2A subunit-containing NMDA receptors in various neurological and pathological disorders. In the X-ray crystal structure, TCN-201 ( 1 ) and analogous pyrazine derivatives 2 and 3 adopt a U-shape (hairpin) conformation within the binding site formed by the ligand binding domains of the GluN1 and GluN2A subunits. In order to mimic the resulting π/π-interactions of two aromatic rings in the binding site, a [2.2]paracyclophane system was designed to lock these aromatic rings in a parallel orientation. Acylation of [2.2]paracyclophane ( 5 ) with oxalyl chloride and chloroacetyl chloride and subsequent transformations led to the oxalamide 7 , triazole 10 and benzamides 12 . The GluN2A inhibitory activities of the paracyclophane derivatives were tested with two-electrode voltage clamp electrophysiology using Xenopus laevis oocytes expressing selectively functional NMDA receptors with GluN2A subunit. The o-iodobenzamide 12 b with the highest similarity to TCN-201 showed the highest GuN2A inhibitory activity of this series of compounds. At a concentration of 10 μM, 12 b reached 36 % of the inhibitory activity of TCN-201 ( 1 ). This result indicates that the [2.2]paracyclophane system is well accepted by the TCN-201 binding site.     

BioPACE

While developing the specifications for electronically enabled Machine Readable Travel Documents, the ICAO paid significant attention to security aspects related to the digital information stored in this type of electronic document. This has resulted in Passive and Active Authentication, Basic Access Control and Extended Access Control. The goal of Basic Access Control (BAC) is preventing unauthorized access to the chip data stored on eMRTDs. The PACE protocol has been developed as a future-proof method to overcome the limitations of BAC and to replace BAC in the future. In this paper the BioPACE protocol is proposed as a supplement to the PACE protocol. Besides verifying if the document holder is indeed the rightful owner, the BioPACE protocol provides a higher convenience for the bearer of the document.