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901.
Christine A Butts Kerry A
C James John P Koolaard Christine L Booth Helen E Donaldson Margaret F Scott Paul J Moughan 《Journal of the science of food and agriculture》2002,82(4):343-350
The effect of time that digesta were collected on the quantity and representativeness of ileal digesta and on the determination of apparent and true ileal nitrogen (N) digestibility for growing rats was investigated. Semi‐synthetic diets containing chromic oxide as an indigestible marker were given to 200 g live weight rats for 8 days as a single daily meal. The experimental treatments comprised six diets (lactalbumin, soy protein isolate, wheat gluten, fish, protein‐free (PF), enzymically hydrolysed casein (EHC)) and four ileal digesta sampling times (3, 4, 5 and 6 h after the start of feeding). On the eighth day the rats were fed and slaughtered according to treatment, and digesta were sampled from the terminal ileum. Endogenous ileal nitrogen excretion was determined using both the protein‐free and peptide alimentation methods. There was a significant (P = 0.027) effect of experimental diet averaged across sampling times for chromium recovery (CrRec). The CrRec was higher for the rats fed the PF diet than for the other diets. Responses to sampling time varied significantly (P < 0.05) among diets for ileal digesta weight (IDW), nitrogen/chromium (N/Cr) ratio, ileal digesta weight relative to dry matter intake (IDW/DMI), CrRec, N flow, apparent N digestibility and true N digestibility (determined using the protein‐free method). The optimal digesta sampling times for each of the diets were 3 h for PF, EHC, lactalbumin and fish and 4 h for soy protein isolate and wheat gluten. Consequently, 3 or 4 h after the start of feeding is recommended as the optimum ileal digesta sampling time for most purified protein sources when fed to the growing rat as a single daily meal. © 2002 Society of Chemical Industry 相似文献
902.
Jessica Andrews George Bullock Cheryl A. Miller Jonathan Booth Hong Ren Nicole L. Kelly John V. Hanna Ian M. Reaney 《Journal of the American Ceramic Society》2023,106(6):3396-3409
Bioactive glasses are widely utilized to regenerate bone tissue and aid bonding of orthopedic implants. Forming composites of bioglass with bioactive polymers allow the mechanical properties and biological response to be tailored. Although several methods for creating bioglass–polymer composites exist, they require dissolution of the polymer, controlled phase separation, and appear to have an upper limit of ∼30 vol.% bioglass. Cold sintering is a novel technique for the densification of ceramics and glasses which utilizes a liquid phase and pressure to allow the production of components at reduced temperatures. We demonstrate that cold sintering (100°C) of Bioglass 45S5 powder produced via flame spray pyrolysis and the fabrication of Bioglass 45S5–polymer composites. Assessment of the in vitro response revealed that composites were not cytotoxic. Solid-state 31P and 29Si MAS NMR studies of the silicon and phosphorus speciation in the glass powder, as-received, wetted, and sintered samples show similarities to reactions expected when bioglass is implanted in the body which along with Raman spectroscopy data gave insight into the cold sintering densification mechanism. 相似文献