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91.
Vlatka Patar Mirela Lozi Nela Kelam Natalija Filipovi Branka Bernard Yu Katsuyama Katarina Vukojevi 《International journal of molecular sciences》2021,22(19)
Disabled-1 (Dab1) protein is an intracellular adaptor of reelin signaling required for prenatal neuronal migration, as well as postnatal neurotransmission, memory formation and synaptic plasticity. Yotari, an autosomal recessive mutant of the mouse Dab1 gene is recognizable by its premature death, unstable gait and tremor. Previous findings are mostly based on neuronal abnormalities caused by Dab1 deficiency, but the role of the reelin signaling pathway in nonneuronal tissues and organs has not been studied until recently. Hepatocytes, the most abundant cells in the liver, communicate via gap junctions (GJ) are composed of connexins. Cell communication disruption in yotari mice was examined by analyzing the expression of connexins (Cxs): Cx26, Cx32, Cx37, Cx40, Cx43 and Cx45 during liver development at 13.5 and 15.5 gestation days (E13.5 and E15.5). Analyses were performed using immunohistochemistry and fluorescent microscopy, followed by quantification of area percentage covered by positive signal. Data are expressed as a mean ± SD and analyzed by one-way ANOVA. All Cxs examined displayed a significant decrease in yotari compared to wild type (wt) individuals at E13.5. Looking at E15.5 we have similar results with exception of Cx37 showing negligible expression in wt. Channels formation triggered by pathological stimuli, as well as propensity to apoptosis, was studied by measuring the expression of Pannexin1 (Panx1) and Apoptosis-inducing factor (AIF) through developmental stages mentioned above. An increase in Panx1 expression of E15.5 yotari mice, as well as a strong jump of AIF in both phases suggesting that yotari mice are more prone to apoptosis. Our results emphasize the importance of gap junction intercellular communication (GJIC) during liver development and their possible involvement in liver pathology and diagnostics where they can serve as potential biomarkers and drug targets. 相似文献
92.
Polyphenol,antioxidant and antimicrobial potential of six different white and red wine grape processing leftovers
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93.
Dinko Novosel Vladimir Brajkovi Mojca Sim
i
Minja Zorc Tanja Svara Karmen Branovic Cakanic Andreja Jungi Betka Logar Vlatka Cubric-Curik Peter Dovc Ino Curik 《International journal of molecular sciences》2022,23(11)
While mitogenome mutations leading to pathological manifestations are rare, more than 200 such mutations have been described in humans. In contrast, pathogenic mitogenome mutations are rare in domestic animals and have not been described at all in cattle. In the small local Slovenian cattle breed Cika, we identified (next-generation sequencing) two cows with the T10432C mitogenome mutation in the ND4L gene, which corresponds to the human T10663C mutation known to cause Leber’s hereditary optic neuropathy (LHON). Pedigree analysis revealed that the cows in which the mutation was identified belong to two different maternal lineages with 217 individual cows born between 1997 and 2020. The identified mutation and its maternal inheritance were confirmed by Sanger sequencing across multiple generations, whereas no single analysis revealed evidence of heteroplasmy. A closer clinical examination of one cow with the T10432C mutation revealed exophthalmos, whereas histopathological examination revealed retinal ablations, subretinal oedema, and haemorrhage. The results of these analyses confirm the presence of mitochondrial mutation T10432C with homoplasmic maternal inheritance as well as clinical and histopathological signs similar to LHON in humans. Live animals with the mutation could be used as a suitable animal model that can improve our understanding of the pathogenesis of LHON and other mitochondriopathies. 相似文献
94.
Dunja Šamec Branimir Urlić Branka Salopek-Sondi 《Critical reviews in food science and nutrition》2019,59(15):2411-2422
ABSTRACTKale (Brassica oleracea var. acephala) is a cruciferous vegetable, characterized by leaves along the stem, which, in recent years, have gained a great popularity as a ´superfood´. Consequently, in a popular culture it is listed in many ´lists of the healthiest vegetables´. Without the doubt, a scientific evidences support the fact that cruciferous vegetables included in human diet can positively affect health and well-being, but remains unclear why kale is declared superior in comparison with other cruciferous. It is questionable if this statement about kale is triggered by scientific evidence or by some other factors. Our review aims to bring an overview of kale's botanical characteristics, agronomic requirements, contemporary and traditional use, macronutrient and phytochemical content and biological activity, in order to point out the reasons for tremendous kale popularity. 相似文献
95.
This paper presents a novel method of PID controller tuning suitable for higher-order aperiodic processes and aimed at step response-based auto-tuning applications. The PID controller tuning is based on the identification of so-called n-th order lag (PTn) process model and application of damping optimum criterion, thus facilitating straightforward algebraic rules for the adjustment of both the closed-loop response speed and damping. The PTn model identification is based on the process step response, wherein the PTn model parameters are evaluated in a novel manner from the process step response equivalent dead-time and lag time constant. The effectiveness of the proposed PTn model parameter estimation procedure and the related damping optimum-based PID controller auto-tuning have been verified by means of extensive computer simulations. 相似文献
96.
Chryssostomos Chatgilialoglu Carla Ferreri Ioannis N. Lykakis Branka Mihaljević 《Israel journal of chemistry》2014,54(3):242-247
Different types of lipid aggregations, such as micelles and liposomes, can be used as biomimetic models. The uses of γ-irradiation as a valid methodology for simulating the biological generation of thiyl radicals in these models are summarized and, in particular, thiyl radical catalyzed cis trans isomerization of unsaturated lipids is underlined. The efficiency of antioxidants against lipid isomerization assayed by biomimetic models and the importance of trans lipids as novel biomarkers of radical stress are also described. 相似文献
97.
Branka B. Radanov Srbislav B. Genić Branislav M. Jaćimović 《American Institute of Chemical Engineers》2016,62(7):2579-2584
This article presents the research results of direct contact condensation of steam on freely formed falling liquid jets. After the comparison of experimental data and open literature correlations it was concluded that published correlations does not provide accurate coverage of experimental data. A new correlation was established in the following form © 2016 American Institute of Chemical Engineers AIChE J, 62: 2579–2584, 2016 相似文献
98.
Nina ?aban Vi?nja Stepani? Sr?an Vu?ini? Anita Horvati? Mario Cindri? Ivana Perkovi? Branka Zorc Nada Or?oli? Mladen Mintas Kre?imir Paveli? Sandra Kraljevi? Paveli? 《International journal of molecular sciences》2013,14(12):23654-23671
The paper presents a detailed study of the biological effects of two amino acid hydroxyurea derivatives that showed selective antiproliferative effects in vitro on the growth of human tumor cell line SW620. Tested compounds induced cell cycle perturbations and apoptosis. Proteins were identified by proteomics analyses using two-dimensional gel electrophoresis coupled to mass spectrometry, which provided a complete insight into the most probable mechanism of action on the protein level. Molecular targets for tested compounds were analyzed by cheminformatics tools. Zinc-dependent histone deacetylases were identified as potential targets responsible for the observed antiproliferative effect. 相似文献
99.
Branka Mihaljević Ivana Tartaro Nada Filipović Vinceković 《European Journal of Lipid Science and Technology》2012,114(11):1292-1303
The effect of molecular environment on the peroxidation of linoleic acid (LA), a polyunsaturated fatty acid (PUFA), initiated by ferrous ions was investigated in acidic and neutral pH conditions. Mixed nonionic surfactants TWEEN®‐20/LA micelles were established as a model system to obtain a surfactant‐in‐lipid aqueous system at high acidity level. The peroxidation of LA was induced by ferrous ions and the kinetics of the produced conjugated dienes was followed by UV measurements and the ferric thiocynate method. Ferrous ions were oxidized only by the preformed LA hydroperoxides, which under established conditions produced lipid alkoxyl and peroxyl radicals as the sole initiators of propagation. The results revealed the LA peroxidation process remained mainly unaffected within the 2.5<pH<5.5 range, while highly pH sensitive around pH 7. The propagation process prevailed at optimal concentrations of 500 µM of LA and 280 µM TWEEN®‐20, and at the ferrous ion concentration up to 75 µM, irrespective of the buffer used. Practical applications: A simple model system in water, suitable for the selective study of the lipid peroxidation propagation phase induced by ferrous ion is presented here. Fatty acids serve as model compounds susceptible to processes associated with oxidative radical initiated‐modifications of lipids. The obtained results contribute to a better understanding of the oxidative behavior of lipids, particularly those soluble in nonionic surfactant micelles in acidic medium. The oxidative stability of the PUFA in model systems containing TWEEN®‐20 and ferrous ion at low pH could be predicted and controlled by measuring the lipid hydroperoxide formation. The experimental conditions presented may also provide a suitable system for the study of the termination phase of lipid peroxidation. 相似文献
100.
Branka Mušič Miha Drofenik Peter Venturini Andrej Žnidaršič 《Ceramics International》2012,38(4):2693-2699
We have investigated an organic resin solution designed for EM wave absorption based on a magnetic filler, composed of phases within the Mn0.66Zn0.27Fe2.07O4 system, embedded in an absorber composite with concentration ratios of 50:50, 75:25 and 90:10 by weight. The formation of the manganese zinc ferrite particles, as the principal magnetic phases, was achieved via the conventional ceramic method. The electromagnetic parameters of the composites were measured with a vector network analyser at 100 MHz to 10 GHz. The subject of the paper was a study of the electromagnetic absorber properties and the rheological properties of the resin composite based on ferrite particles with respect to using the materials in architectural coatings. 相似文献