Path-breaking books in regional science

This article presents a collection of regional science books that longstanding members of the Regional Science Association International (RSAI) identified as path-breaking books. The most frequently nominated books include the classics by Isard, the seminal books in urban economics by Alonso, Muth and Mills, methods books by Miernyk, Wilson, Anselin, and Cliff and Ord, textbooks by Beckmann and Richardson, as well as the recent contribution by Fujita, Krugman and Venables. Reviews of these books, written by leading scholars from different continents, make up the major contribution of this article and are a testimony to the far-reaching influence of regional science in the academic literature.JEL Classification: A2, B2, B3, C00, R00With contributions by Michael Batty, Manas Chatterji, Kieran Donaghy, Bernard Fingleton, Arthur Getis, Michael J. Greenwood, Daniel P. McMillen, Gordon F. Mulligan, Jan Oosterhaven,Peter V. Schaeffer, Daniel Shefer. See the Appendix for contributors affiliations and contact information.     

Effect of ethanol on the growth of Clostridium botulinum

Model broth studies were carried out to investigate the effect of ethanol on the growth of proteolytic (group I) strains of Clostridium botulinum. Ethanol extended the pathogen's lag phase, decreased its exponential growth rate, and decreased its final level of growth in the stationary phase. In all cases, botulinum neurotoxin production was associated with growth. Micrographs of C. botulinum cultures grown at 37 degrees C in trypticase peptone glucose yeast extract (TPGY) broths containing 2 and 4% ethanol showed elongation of vegetative cells and interference with cell division. The inhibition of growth and toxin production at the ethanol level predicted (5.5%, wt/wt) was confirmed by microscopy and by the mouse bioassay. A subsequent study was carried out to determine the combined effect of ethanol (0 to 8% [wt/wt]), water activity (aw; 0.953 to 0.997), and pH (6.2 to 8.2) on the probability of the growth of and neurotoxin production by proteolytic strains of C. botulinum (10(3) spores per ml). Growth and neurotoxin production occurred in 1 to 3 days in TPGY broths without ethanol (0%) and in 2 to 4 days in broths containing 2% ethanol regardless of the aw or pH levels (P < 0.005). Growth and neurotoxin production were delayed by an ethanol concentration of 4% ethanol and completely inhibited by a concentration of 6%. At an ethanol concentration of 4%, the probability of growth and toxin production over 365 days (Pt) was influenced by aw and pH. After 365 days, the maximum probability of growth and toxin production (Pmax) was 1 for all but one combination. However, tau, the time it took for 50% of all eventually positive replicates for any given combination of barriers to show growth and/or turbidity, ranged from <3 to 229 days. All tubes of TPGY broths that showed no growth after 365 days were subcultured in fresh TPGY broths. In all cases, growth and toxin production occurred within 24 h at 37 degrees C, indicating the reversible (sporostatic and/or bacteriostatic) effect of ethanol on C. botulinum.     

Capsosomes with Multilayered Subcompartments: Assembly and Loading with Hydrophobic Cargo

Therapeutic artificial cells or organelles are nanoengineered vehicles that are expected to substitute for missing or lost cellular function. The creation of capsosomes, polymer carrier capsules containing liposomal subcompartments, is a promising approach towards constructing such therapeutic devices using the layer‐by‐layer assembly method. Herein, the assembly of intact, nonaggregated capsosomes containing multiple liposome layers is reported. It is also further demonstrated that thiocoraline, a hydrophobic model peptide with antitumor activity, can be efficiently loaded into the membrane of the liposomal subcompartments of the capsosomes. Cell viability assays verify the activity of the trapped antitumor cargo. It is also shown that pristine capsosomes do not display inherent cytotoxic effects. The ability to tune the number of liposome layers and hence the drug loading in capsosomes as well as their noncytotoxicity provide new opportunities for the creation of therapeutic artificial cells and organelles.     

A diagnosis-based approach for tire-road forces and maximum friction estimation

A new approach to estimate vehicle tire forces and road maximum adherence is presented. Contrarily to most of the previous works on this subject, it is not an asymptotic observer-based estimation, but a combination of elementary diagnosis tools and new algebraic techniques for filtering and estimating derivatives of noisy signals. In a first step, instantaneous friction and lateral forces will be computed within this framework. Then, extended braking stiffness concept is exploited to detect which braking efforts allow to distinguish a road type from another. A weighted Dugoff model is used during these ‘distinguishable’ intervals to estimate the maximum friction coefficient. Very promising results have been obtained in noisy simulations and real experimentations for most of the driving situations.     

Algorithms for the maximum satisfiability problem

Old and new algorithms for the Maximum Satisfiability problem are studied. We first summarize the different heuristics previously proposed, i.e., the approximation algorithms of Johnson and of Lieberherr for the general Maximum Satisfiability problem, and the heuristics of Lieberherr and Specker, Poljak and Turzik for the Maximum 2-Satisfiability problem. We then consider two recent local search algorithmic schemes, the Simulated Annealing method of Kirkpatrick, Gelatt and Vecchi and the Steepest Ascent Mildest Descent method, and adapt them to the Maximum Satisfiability problem. The resulting algorithms, which avoid being blocked as soon as a local optimum has been found, are shown empirically to be more efficient than the heuristics previously proposed in the literature.     

Initiation of cationic polymerization by tetramethylene zwitterions from tetracyanocyclobutanes

The initiating ability of tetramethylene zwitterions formed from cyclobutane adducts of donor olefins with TCNE was investigated. Polar solvents increased the ability of vinyl ether-TCNE cyclobutane adducts to initiate the cationic polymerization of N-vinylcarbazole. The concept of charge separation in the tetramethylene zwitterions was also investigated.     

The Tyrosine Gate of the Bacterial Lectin FimH: A Conformational Analysis by NMR Spectroscopy and X‐ray Crystallography

Urinary tract infections caused by uropathogenic E. coli are among the most prevalent infectious diseases. The mannose‐specific lectin FimH mediates the adhesion of the bacteria to the urothelium, thus enabling host cell invasion and recurrent infections. An attractive alternative to antibiotic treatment is the development of FimH antagonists that mimic the physiological ligand. A large variety of candidate drugs have been developed and characterized by means of in vitro studies and animal models. Here we present the X‐ray co‐crystal structures of FimH with members of four antagonist classes. In three of these cases no structural data had previously been available. We used NMR spectroscopy to characterize FimH–antagonist interactions further by chemical shift perturbation. The analysis allowed a clear determination of the conformation of the tyrosine gate motif that is crucial for the interaction with aglycone moieties and was not obvious from X‐ray structural data alone. Finally, ITC experiments provided insight into the thermodynamics of antagonist binding. In conjunction with the structural information from X‐ray and NMR experiments the results provide a mechanism for the often‐observed enthalpy–entropy compensation of FimH antagonists that plays a role in fine‐tuning of the interaction.     

Design of survivable WDM networks using pre-configured protection structures with unrestricted shapes

We propose a novel protection approach for the design of link-protection schemes in survivable Wavelength Division Multiplexing mesh networks by merging the well-known p-cycle- and p-tree-protection structures. So doing, we aim at gathering the advantages of p-cycles in terms of protection capabilities, and of p-trees in terms of protection flexibilities (local re-routing, scalability) in a single protection scheme. As opposed to existing protection schemes based on protection structures with a pre-defined shape, the building blocks of the new scheme are protection structures with unrestricted shapes. Thus, they allow more flexibility in provisioning spare capacity, and provide higher capacity efficiency when compared to the shaped-protection schemes that have been proposed so far. In order to cope with the size of the solution space which includes all the possible protection structures, we propose an efficient and scalable optimization technique in large-scale systems named column generation (CG). In our CG-based optimization approach, the shape of a candidate protection structure is dynamically decided during the optimization process according to a link spare capacity budget. Experimental results on different network instances show that the protection plan resulting from the merging of p-cycle and p-tree structures is, on average, ~15% less capacity redundant and ~15% more reliable than the pure p-cycle one. It also requires, on average, ~30% less protection structures. In addition, those structures provide backup paths ~30% smaller than those of the p-cycle-based scheme.     

Use of volatile compound metabolic signatures in poultry liver to back-trace dietary exposure to rapidly metabolized xenobiotics

The study investigated the feasibility of using volatile compound signatures of liver tissues in poultry to detect previous dietary exposure to different types of xenobiotic. Six groups of broiler chickens were fed a similar diet either noncontaminated or contaminated with polychlorinated dibenzo-p-dioxins/-furans (PCDD/Fs; 3.14 pg WHO-TEQ/g feed, 12% moisture), polychlorinated biphenyls (PCBs; 0.08 pg WHO-TEQ/g feed, 12% moisture), polybrominated diphenyl ethers (PBDEs; 1.63 ng/g feed, 12% moisture), polycyclic aromatic hydrocarbons (PAHs; 0.72 μg/g fresh matter), or coccidiostats (0.5 mg/g feed, fresh matter). Each chicken liver was analyzed by solid-phase microextraction - mass spectrometry (SPME-MS) for volatile compound metabolic signature and by gas chromatography - high resolution mass spectrometry (GC-HRMS), gas chromatography - tandem mass spectrometry (GC-MS/MS), and liquid chromatography - tandem mass spectrometry (LC-MS/MS) to quantify xenobiotic residues. Volatile compound signature evidenced a liver metabolic response to PAH although these rapidly metabolized xenobiotics are undetectable in this organ by the reference methods. Similarly, the volatile compound metabolic signature enabled to differentiate the noncontaminated chickens from those contaminated with PBDEs or coccidiostats. In contrast, no clear signature was pointed out for slowly metabolized compounds such as PCDD/Fs and PCBs although their residues were found in liver at 50.93 (±6.71) and 0.67 (±0.1) pg WHO-TEQ/g fat, respectively.