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排序方式: 共有235条查询结果,搜索用时 78 毫秒
31.
Immunological properties of human decidual macrophages--a possible role in intrauterine immunity 总被引:2,自引:0,他引:2
Singh U Nicholson G Urban BC Sargent IL Kishore U Bernal AL 《Reproduction (Cambridge, England)》2005,129(5):631-637
Our aim was to investigate the contribution of decidual macrophages, which constitute an important immune component of the decidua in late gestation, to intrauterine defence mechanisms. Using flow cytometry we examined the ability of decidual macrophages, isolated from term decidua, to bind and phagocytose fluorescence-labelled bacterial and yeast bioparticles. We also assessed their ability to generate superoxide radicals and tumour necrosis factor-alpha following lipopolysaccharide challenge. Decidual macrophages bound bacterial and yeast particles in a dose-dependent manner, which subsequently led to phagocytosis. These macrophages also produced superoxide radicals and the pro-inflammatory cytokine TNF-alpha when challenged with bacterial lipopolysaccharides. These results suggest a role for decidual macrophages in pathogen recognition and clearance during pregnancy, and, therefore, they are likely to protect the fetus against intrauterine infections which might otherwise lead to preterm labour. 相似文献
32.
Validation of the performance of a GMO multiplex screening assay based on microarray detection 总被引:1,自引:1,他引:0
Serge Leimanis Sandrine Hamels Florence Nazé Guillaume Mbongolo Mbella Myriam Sneyers Rupert Hochegger Hermann Broll Lillian Roth Klára Dallmann Adrienn Micsinai José Luis La Paz Maria Pla Claudia Brünen-Nieweler Nina Papazova Isabel Taverniers Norbert Hess Britta Kirschneit Yves Bertheau Colette Audeon Valérie Laval Ulrich Busch Sven Pecoraro Katrin Neumann Sibylle Rösel Jeroen van Dijk Esther Kok Gianni Bellocchi Nicoletta Foti Marco Mazzara William Moens José Remacle Guy Van Den Eede 《European Food Research and Technology》2008,227(6):1621-1632
A new screening method for the detection and identification of GMO, based on the use of multiplex PCR followed by microarray, has been developed and is presented. The technology is based on the identification of quite ubiquitous GMO genetic target elements first amplified by PCR, followed by direct hybridisation of the amplicons on a predefined microarray (DualChip® GMO, Eppendorf, Germany). The validation was performed within the framework of a European project (Co-Extra, contract no 007158) and in collaboration with 12 laboratories specialised in GMO detection. The present study reports the strategy and the results of an ISO complying validation of the method carried out through an inter-laboratory study. Sets of blind samples were provided consisting of DNA reference materials covering all the elements detectable by specific probes present on the array. The GMO concentrations varied from 1% down to 0.045%. In addition, a mixture of two GMO events (0.1% RRS diluted in 100% TOPAS19/2) was incorporated in the study to test the robustness of the assay in extreme conditions. Data were processed according to ISO 5725 standard. The method was evaluated with predefined performance criteria with respect to the EC CRL method acceptance criteria. The overall method performance met the acceptance criteria; in particular, the results showed that the method is suitable for the detection of the different target elements at 0.1% concentration of GMO with a 95% accuracy rate. This collaborative trial showed that the method can be considered as fit for the purpose of screening with respect to its intra- and inter-laboratory accuracy. The results demonstrated the validity of combining multiplex PCR with array detection as provided by the DualChip® GMO (Eppendorf, Germany) for the screening of GMO. The results showed that the technology is robust, practical and suitable as a screening tool. 相似文献
33.
Fängström B Athanassiadis I Odsjö T Norén K Bergman A 《Molecular nutrition & food research》2008,52(2):187-193
Environmental and human exposures to brominated flame retardants (BFR) have been of emerging concern since some BFR are persistent and bioaccumulative compounds. Among those, polybrominated diphenyl ethers (PBDE) have frequently been reported in low to high ng/g concentrations in human blood around the world while hexabromocyclododecane (HBCDD) only occasionally has been reported and then in the low ppb concentrations in human blood. The present study concerns PBDE congener and HBCDD concentrations in human milk from Stockholm from 1980 to 2004. HBCDD concentrations has increased four to five times since 1980 until 2002 but seems to have stabilized at this concentration in the last years (2003/04). Similarly, BDE-153 has continued to increase at least to 2001, after which it has stabilized in the mother's milk. Other PBDE congeners with four to five bromine substituents peaked 5 years earlier (1995) and are all decreasing. DecaBDE (BDE-209) is not a suitable biomarker for time trend studies according to the present results, showing no changes over time. This is likely due to its short apparent half-life in humans and poor transfer from blood to milk. 相似文献
34.
Fisher JC Wallschläger D Planer-Friedrich B Hollibaugh JT 《Environmental science & technology》2008,42(1):81-85
Sulfur and arsenic often coexist in the environment and share similar microbial redox transformations. We examined the effects of sulfide on aerobic arsenite oxidation in alkaline lake water samples and in laboratory enrichment cultures. Significant arsenite oxidation occurred only in treatments with bacteria present, and production of arsenate was greatly enhanced by the addition of sulfide or thiosulfate. IC-ICP-MS analysis of samples showed that mono- and dithioarsenate formed in arsenite + sulfide amended lake water. Our data indicate that these two thioarsenic compounds are fairly stable in sterile alkaline solutions, but are transformed predominantly to arsenate when bacteria are present. Enrichment culture experiments suggest that sulfur-oxidizing bacteria use free or arsenic-bound sulfur as a growth substrate and directly or indirectly transform arsenite and thioarsenates to arsenate during growth. Increases in cell density resulted in more rapid conversion of arsenite and thioarsenates. The rate and extent of these processes appearto be controlled bythe concentration of bacteria and the ratio of reduced sulfur to arsenite present. Sulfur-driven arsenite oxidation and microbial thioarsenate transformation may be important biogeochemical processes in the arsenic cycle of our study site (Mono Lake, CA, USA) and other alkaline environments as well. 相似文献
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von der Lühe Barbara Pauli Laura Greenshields Britta Hughes Harold J. Tjoa Aiyen Sauer Daniela 《SILICON》2021,13(12):4345-4353
Silicon - The effects of land use and fire on ecosystem silicon (Si) cycling has been largely disregarded so far. We investigated the impacts of land use and fire on Si release from topsoils and... 相似文献
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Dargahi Noubary Kaveh Kellner Michael Hötzer Johannes Seiz Marco Seifert Hans J. Nestler Britta 《Journal of Materials Science》2021,56(20):11932-11952
Journal of Materials Science - In order to approximate Gibbs energy functions, a semi-automated framework is introduced for binary and ternary material systems, using Calphad databases. To generate... 相似文献
40.
Patterning: Patterning of Soft Matter across Multiple Length Scales (Adv. Funct. Mater. 16/2016)
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