全文获取类型
收费全文 | 83446篇 |
免费 | 15817篇 |
国内免费 | 156篇 |
专业分类
电工技术 | 1416篇 |
综合类 | 69篇 |
化学工业 | 26602篇 |
金属工艺 | 2468篇 |
机械仪表 | 4001篇 |
建筑科学 | 2713篇 |
矿业工程 | 26篇 |
能源动力 | 2889篇 |
轻工业 | 10397篇 |
水利工程 | 545篇 |
石油天然气 | 140篇 |
武器工业 | 2篇 |
无线电 | 13323篇 |
一般工业技术 | 20601篇 |
冶金工业 | 4393篇 |
原子能技术 | 674篇 |
自动化技术 | 9160篇 |
出版年
2024年 | 50篇 |
2023年 | 574篇 |
2022年 | 882篇 |
2021年 | 1683篇 |
2020年 | 2340篇 |
2019年 | 3965篇 |
2018年 | 4200篇 |
2017年 | 4436篇 |
2016年 | 5192篇 |
2015年 | 4828篇 |
2014年 | 5522篇 |
2013年 | 7620篇 |
2012年 | 5717篇 |
2011年 | 6032篇 |
2010年 | 5227篇 |
2009年 | 5227篇 |
2008年 | 4684篇 |
2007年 | 3913篇 |
2006年 | 3548篇 |
2005年 | 2985篇 |
2004年 | 2818篇 |
2003年 | 2717篇 |
2002年 | 2499篇 |
2001年 | 2153篇 |
2000年 | 2004篇 |
1999年 | 1359篇 |
1998年 | 1601篇 |
1997年 | 1040篇 |
1996年 | 823篇 |
1995年 | 573篇 |
1994年 | 487篇 |
1993年 | 421篇 |
1992年 | 306篇 |
1991年 | 289篇 |
1990年 | 271篇 |
1989年 | 249篇 |
1988年 | 209篇 |
1987年 | 176篇 |
1986年 | 128篇 |
1985年 | 119篇 |
1984年 | 95篇 |
1983年 | 66篇 |
1982年 | 38篇 |
1981年 | 40篇 |
1980年 | 31篇 |
1979年 | 31篇 |
1978年 | 33篇 |
1977年 | 40篇 |
1976年 | 61篇 |
1973年 | 20篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
31.
Jeongchan Lee Dr. Joonwon Kim Hyun Kim Eun-Jung Kim Prof. Dr. Hee-Jin Jeong Prof. Dr. Kwon-Young Choi Prof. Dr. Byung-Gee Kim 《Chembiochem : a European journal of chemical biology》2020,21(10):1446-1452
Tryptophan halogenases are found in diverse organisms and catalyze regiospecific halogenation. They play an important role in the biosynthesis of halogenated indole alkaloids, which are biologically active and of therapeutic importance. Here, a tryptophan 6-halogenase (SatH) from Streptomyces albus was characterized by using a whole-cell reaction system in Escherichia coli. SatH showed substrate specificity for chloride and bromide ions, leading to regiospecific halogenation at the C6-position of l -tryptophan. In addition, SatH exhibited higher performance in bromination than that of previously reported tryptophan halogenases in the whole-cell reaction system. Through structure-based protein mutagenesis, it has been revealed that two consecutive residues, A78/V79 in SatH and G77/I78 in PyrH, are key determinants in the regioselectivity difference between tryptophan 6- and 5-halogenases. Substituting the AV with GI residues switched the regioselectivity of SatH by moving the orientation of tryptophan. These data contribute to an understanding of the key residues that determine the regioselectivity of tryptophan halogenases. 相似文献
32.
Towards a Better Prediction of Cell Settling on Nanostructure Arrays—Simple Means to Complicated Ends
下载免费PDF全文
![点击此处可从《Advanced functional materials》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Nina Buch‐Månson Sara Bonde Jessica Bolinsson Trine Berthing Jesper Nygård Karen L. Martinez 《Advanced functional materials》2015,25(21):3246-3255
Vertical arrays of nanostructures (NSs) are emerging as promising platforms for probing and manipulating live mammalian cells. The broad range of applications requires different types of interfaces, but cell settling on NS arrays is not yet fully controlled and understood. Cells are both seen to deform completely into NS arrays and to stay suspended like tiny fakirs, which have hitherto been explained with differences in NS spacing or density. Here, a better understanding of this phenomenon is provided by using a model that takes into account the extreme membrane deformation needed for a cell to settle into a NS array. It is shown that, in addition to the NS density, cell settling depends strongly on the dimensions of the single NS, and that the settling can be predicted for a given NS array geometry. The predictive power of the model is confirmed by experiments and good agreement with cases from the literature. Furthermore, the influence of cell‐related parameters is evaluated theoretically and a generic method of tuning cell settling through surface coating is demonstrated experimentally. These findings allow a more rational design of NS arrays for the numerous exciting biological applications where the mode of cell settling is crucial. 相似文献
33.
34.
Fundamental Evaluation of Power Supply and Rectifiers for Wireless Power Transfer Using Magnetic Resonant Coupling
下载免费PDF全文
![点击此处可从《Electrical Engineering in Japan》网站下载免费的PDF全文](/ch/ext_images/free.gif)
This paper provides a fundamental analysis of a power supply and rectifiers for wireless power transfer using magnetic resonant coupling (MRC). MRC enables efficient wireless power transfer over middle‐range transfer distances. MRC for wireless power transfer should operate at a high frequency in the industry science medical band, such as 13.56 MHz, because the size of the transfer device decreases at higher transfer frequencies. Therefore, the output frequency of the power supply on the transmitting side should be 13.56 MHz. In addition, the rectifier on the receiving side is operated at a high frequency. This paper focuses on the reflected power in the power supply and rectifiers. Thus, the parametric design method is clarified for the power supply, including a low‐pass filter to match the output, the impedance of the power supply with the characteristic impedance of the transmission line. In addition, the effects on the rectifiers of silicon carbide and gallium nitride diodes are confirmed by performing an experiment and a loss analysis. 相似文献
35.
Choi Paul Santos Christian Ariane Kim Min-Kun Jung Hyunsook Hong Do-Young Koo Junemo 《Journal of Mechanical Science and Technology》2021,35(11):5007-5022
Journal of Mechanical Science and Technology - This study delivers equations useful for low-height pleated fibrous filter design: two pressure drop equations and one set of optimum design equations... 相似文献
36.
37.
Kazuo Tsubota Stephen C. Pflugfelder Zuguo Liu Christophe Baudouin Hyo Myung Kim Elisabeth M. Messmer Friedrich Kruse Lingyi Liang Jimena Tatiana Carreno-Galeano Maurizio Rolando Norihiko Yokoi Shigeru Kinoshita Reza Dana 《International journal of molecular sciences》2020,21(23)
Over the past decades, the number of patients with dry eye disease (DED) has increased dramatically. The incidence of DED is higher in Asia than in Europe and North America, suggesting the involvement of cultural or racial factors in DED etiology. Although many definitions of DED have been used, discrepancies exist between the various definitions of dry eye disease (DED) used across the globe. This article presents a clinical consensus on the definition of DED, as formulated in four meetings with global DED experts. The proposed new definition is as follows: “Dry eye is a multifactorial disease characterized by a persistently unstable and/or deficient tear film (TF) causing discomfort and/or visual impairment, accompanied by variable degrees of ocular surface epitheliopathy, inflammation and neurosensory abnormalities.” The key criteria for the diagnosis of DED are unstable TF, inflammation, ocular discomfort and visual impairment. This definition also recommends the assessment of ocular surface epitheliopathy and neurosensory abnormalities in each patient with suspected DED. It is easily applicable in clinical practice and should help practitioners diagnose DED consistently. This consensus definition of DED should also help to guide research and clinical trials that, to date, have been hampered by the lack of an established surrogate endpoint. 相似文献
38.
Nazir Kizzie‐Hayford Doris Jaros Yvonne Schneider Harald Rohm 《International Journal of Food Science & Technology》2015,50(2):381-388
A standard method for the extraction of tiger nut milk has been introduced. It has been shown that, although milling duration improves the yield of tiger nut milk solids and its nutrient composition, there is a quantifiable loss of nutrient in the pressing residue during milk extraction. Milling duration improved the colloidal stability of the milk against creaming during 16 h of storage. A higher milling intensity resulted in the aggregation of biological polymers which resulted in colloidal destabilisation. Milling improved the lightness and stability and reduced browning rate of the tiger nut milk during storage. This report is important for the production of tiger nut milk of consistent and comparable characteristics. Milling has been introduced as a processing method for the qualitative and quantitative modulation of the properties of tiger nut milk. It is recommended to develop further strategies to improve the colloidal stability of tiger nut milk as a beverage. 相似文献
39.
40.