Nature‐Inspired Design and Application of Lipidic Lyotropic Liquid Crystals

Amphiphilic lipids aggregate in aqueous solution into a variety of structural arrangements. Among the plethora of ordered structures that have been reported, many have also been observed in nature. In addition, due to their unique morphologies, the hydrophilic and hydrophobic domains, very high internal interfacial surface area, and the multitude of possible order?order transitions depending on environmental changes, very promising applications have been developed for these systems in recent years. These include crystallization in inverse bicontinuous cubic phases for membrane protein structure determination, generation of advanced materials, sustained release of bioactive molecules, and control of chemical reactions. The outstanding diverse functionalities of lyotropic liquid crystalline phases found in nature and industry are closely related to the topology, including how their nanoscopic domains are organized. This leads to notable examples of correlation between structure and macroscopic properties, which is itself central to the performance of materials in general. The physical origin of the formation of the known classes of lipidic lyotropic liquid crystalline phases, their structure, and their occurrence in nature are described, and their application in materials science and engineering, biology, medical, and pharmaceutical products, and food science and technology are exemplified.     

Intersatellite range monitoring using optical interferometry

We report on an interferometer designed to provide 1-10 nm/square root(Hz) displacement measurement resolution, in the range 0.01 Hz to 1 Hz, while in low Earth orbit. The interferometer comprises two units, each with its own laser and in separate satellites, which would be in the same orbit separated by approximately 50 km. We discuss the requirements on the interferometer subsystem and describe the optical transponder distance measurement, including a phase locking method to generate a heterodyne beat signal between the two lasers. Design, fabrication, and testing of a "flightlike" engineering model interferometer is outlined, and results from environmental and performance tests are reported.     

Lump localisation through a deformation-based tactile feedback system using a biologically inspired finger sensor

The ability to localise harder areas in soft tissues is often desired during robot-assisted surgical operations. A deformation-based tactile feedback system was tested for the detection of objects within soft tissues, after being chosen over common pressure-based designs. This system uses a biologically inspired sensor that offers a new finger-like approach to tactile sensing. A tactile shape display developed from previous successful designs was used to output the sensed tactile information. Using the tactile feedback system on a mechanical teleoperated device, test subjects palpated a number of artificial tissue models to locate objects of varying stiffness. The addition of the tactile feedback system improved the detection of the objects from 64% to 98%, reduced the localisation error from 18 to 11 mm, and also decreased the time the users spent palpating the tissue from 55 to 37 s. This demonstrates that a deformation-based tactile feedback system can be used to successfully locate hard embedded objects within soft tissue, with a significant improvement over force and visual feedback alone. During testing, it was found that the users were able to more accurately locate the softest embedded objects compared to stiffer ones. Reasons for this observation are discussed.     

Limit Dextrinase — Does Its Malt Activity Relate to Its Activity During Brewing?

Limit dextrinase (EC 3.2.1.142) hydrolyses α‐1,6 glucosidic bonds in amylopectin and branched dextrins. Measurement of limit dextrinase activity during fermentation of unboiled wort at the pH of the wort has shown that its activity increases almost 10 fold during the first 10–15 h of fermentation and this increase in activity is unaffected by the presence of leupeptin, a cysteine protease inhibitor. The increase in activity seen when assays were carried out at pH 5.5 was much smaller and was reduced by leupeptin. The activity of limit dextrinase declined slowly during the latter part of the fermentation. It was established that the optimum pH for rapid extraction and assay of malt limit dextrinase in the absence of a reducing agent is approximately 4.5, but in the presence of dithiothreitol, at pH 5.5, activities 2–3 times higher can be obtained after 5 h extraction (600–700 mU/g dry weight). Limit dextrinase activities after 1 h extraction at mashing temperatures were below 20 mU/g dry weight if the mash pH was below 5.0. It is concluded that at pHs below 5.0, where limit dextrinase activity is below its optimum for activity, limit dextrinase activity increases due to dissociation of the inhibitor/enzyme complex. The protection from mashing temperatures of 65°C afforded by the inhibitor is lost at these lower pHs.     

Impedance spectra analysis to characterize interdigitated electrodes as electrochemical sensors

Interdigitated electrode (IDE) arrays have been used widely for electrochemical impedance spectroscopy (EIS). Here, we present an in-depth analysis of interdigitated electrode impedance spectra. Such an analysis is necessary for the identification of the contribution of each interdigitated electrode circuit element to the impedance change. We also discuss the importance of the solution conductivity in impedance spectroscopy showing that the use of low conductivity solutions is advantageous when inter-digit solution resistance and geometric capacitance need to be monitored.     

Sugar fatty acid ester surfactants: Biodegradation pathways

In previous work, we found that the presence of a sulfonyl or alkyl group adjacent to the ester bond of sugar ester surfactants is associated with a dramatic reduction in the rate of biodegradation relative to that of unsubstituted esters. In this study, we investigated the pathways followed during the biodegradation of sucrose laurate, sucrose α-sulfonyl laurate, and sucrose α-ethyl laurate to determine the reasons for their different biodegradation rates. Through the use of high-performance liquid chromatography and proton nuclear magnetic resonance spectroscopy, the nature of the intermediates formed during the biodegradation of these three key sugar esters was determined. It was found that sucrose laurate biodegradation occurs via initial ester hydrolysis. In contrast, sucrose α-sulfonyl laurate degrades by initial alkyl chain oxidation. This indicates that the ester hydrolysis pathway is blocked by the sulfonyl group adjacent to the ester bond so that biodegradation is forced to proceed via the slower alkyl chain oxidation pathway. Sucrose α-ethyl laurate was degraded at least in part by alkyl chain oxidation, indicating that ester hydrolysis was also inhibited by the presence of an ethyl group. It is therefore concluded that previously observed relationships between structure and biodegradability arise because of the influence that different structural elements have on the pathways followed during biodegradation.     

Mitogen and Stress-Activated Kinases 1 and 2 Mediate Endothelial Dysfunction

Inflammation promotes endothelial dysfunction, but the underlying mechanisms remain poorly defined in vivo. Using translational vascular function testing in myocardial infarction patients, a situation where inflammation is prevalent, and knock-out (KO) mouse models we demonstrate a role for mitogen-activated-protein-kinases (MAPKs) in endothelial dysfunction. Myocardial infarction significantly lowers mitogen and stress kinase 1/2 (MSK1/2) expression in peripheral blood mononuclear cells and diminished endothelial function. To further understand the role of MSK1/2 in vascular function we developed in vivo animal models to assess vascular responses to vasoactive drugs using laser Doppler imaging. Genetic deficiency of MSK1/2 in mice increased plasma levels of pro-inflammatory cytokines and promoted endothelial dysfunction, through attenuated production of nitric oxide (NO), which were further exacerbated by cholesterol feeding. MSK1/2 are activated by toll-like receptors through MyD88. MyD88 KO mice showed preserved endothelial function and reduced plasma cytokine expression, despite significant hypercholesterolemia. MSK1/2 kinases interact with MAPK-activated proteins 2/3 (MAPKAP2/3), which limit cytokine synthesis. Cholesterol-fed MAPKAP2/3 KO mice showed reduced plasma cytokine expression and preservation of endothelial function. MSK1/2 plays a significant role in the development of endothelial dysfunction and may provide a novel target for intervention to reduce vascular inflammation. Activation of MSK1/2 could reduce pro-inflammatory responses and preserve endothelial vasodilator function before development of significant vascular disease.     

Effect of organic loading rate on the biological performance of the thermophilic anaerobic membrane bioreactor treating pulp and paper primary sludge

Clean Technologies and Environmental Policy - Waste-to-energy or value-added products have been increasingly considered in many pulp and paper mills (PPMs) worldwide. However, developing...     

South Pole Telescope optics

The South Pole Telescope is a 10 m diameter, wide-field, offset Gregorian telescope with a 966-pixel, millimeter-wave, bolometer array receiver. The telescope has an unusual optical system with a cold stop around the secondary. The design emphasizes low scattering and low background loading. All the optical components except the primary are cold, and the entire beam from prime focus to the detectors is surrounded by cold absorber.     

Direct Assembly of Vertically Oriented,Gold Nanorod Arrays

Although many nanoscale materials such as quantum dots and metallic nanocrystals exhibit size dependent optical properties, it has been difficult to incorporate them into optical or electronic devices because there are currently no methods for precise, large-scale deposition of single nanocrystals. Of particular interest is the need to control the orientation of single nanocrystals since the optical properties are usually strongly anisotropic. Here a method based on electrophoretic deposition (EPD) is reported to precisely assemble vertically oriented, single gold nanorods. It is demonstrated that the orientation of gold nanorods during deposition is controlled by the electric dipole moment induced along the rod by the electric field. Dissipative particle dynamics simulations indicate that the magnitude of this dipole moment is dominated by the polarizability of the solution phase electric double layer around the nanorod. The resulting vertical gold nanorod arrays exhibit reflected colors due to selective excitation of the transverse surface plasmon mode. The EPD method allows assembly of arrays with a density of over one million, visually resolvable, vertical nanorods per square millimeter.