Col4a3-/- Mice on Balb/C Background Have Less Severe Cardiorespiratory Phenotype and SGLT2 Over-Expression Compared to 129x1/SvJ and C57Bl/6 Backgrounds

Alport syndrome (AS) is a hereditary renal disorder with no etiological therapy. In the preclinical Col4a3^-/- model of AS, disease progression and severity vary depending on mouse strain. The sodium-glucose cotransporter 2 (SGLT2) is emerging as an attractive therapeutic target in cardiac/renal pathologies, but its application to AS remains untested. This study investigates cardiorespiratory function and SGLT2 renal expression in Col4a3^-/- mice from three different genetic backgrounds, 129x1/SvJ, C57Bl/6 and Balb/C. male Col4a3^-/- 129x1/SvJ mice displayed alterations consistent with heart failure with preserved ejection fraction (HFpEF). Female, but not male, C57Bl/6 and Balb/C Col4a3^-/- mice exhibited mild changes in systolic and diastolic function of the heart by echocardiography. Male C57Bl/6 Col4a3^-/- mice presented systolic dysfunction by invasive hemodynamic analysis. All strains except Balb/C males demonstrated alterations in respiratory function. SGLT2 expression was significantly increased in AS compared to WT mice from all strains. However, cardiorespiratory abnormalities and SGLT2 over-expression were significantly less in AS Balb/C mice compared to the other two strains. Systolic blood pressure was significantly elevated only in mutant 129x1/SvJ mice. The results provide further evidence for strain-dependent cardiorespiratory and hypertensive phenotype variations in mouse AS models, corroborated by renal SGLT2 expression, and support ongoing initiatives to develop SGLT2 inhibitors for the treatment of AS.     

Circadian Rhythm Dysregulation and Leukemia Development: The Role of Clock Genes as Promising Biomarkers

The circadian clock (CC) is a daily system that regulates the oscillations of physiological processes and can respond to the external environment in order to maintain internal homeostasis. For the functioning of the CC, the clock genes (CG) act in different metabolic pathways through the clock-controlled genes (CCG), providing cellular regulation. The CC’s interruption can result in the development of different diseases, such as neurodegenerative and metabolic disorders, as well as cancer. Leukemias correspond to a group of malignancies of the blood and bone marrow that occur when alterations in normal cellular regulatory processes cause the uncontrolled proliferation of hematopoietic stem cells. This review aimed to associate a deregulated CC with the manifestation of leukemia, looking for possible pathways involving CG and their possible role as leukemic biomarkers.     

Intra- and interspecific mineral composition variability of commercial instant coffees and coffee substitutes: Contribution to mineral intake

The present paper reports the amount and estimated daily mineral intake of nine elements (Ca, Mg, K, Na, P, Fe, Mn, Cr and Ni) in commercial instant coffees and coffee substitutes (n = 49). Elements were quantified by high-resolution continuum source flame (HR-CS-FAAS) and graphite furnace (HR-CS-GFAAS) atomic absorption spectrometry, while phosphorous was evaluated by a standard vanadomolybdophosphoric acid colorimetric method.Instant coffees and coffee substitutes are rich in K, Mg and P (>100 mg/100 g dw), contain Na, Ca and Fe in moderate amounts (>1 mg/100 g), and trace levels of Cr and Ni. Among the samples analysed, plain instant coffees are richer in minerals (p < 0.001), except for Na and Cr. Blends of coffee substitutes (barley, malt, chicory and rye) with coffee (20–66%) present intermediate amounts, while lower quantities are found in substitutes without coffee, particularly in barley.From a nutritional point of view the results indicate that the mean ingestion of two instant beverages per day (total of 4 g instant powder), either with or without coffee, cannot be regarded as important sources of minerals to the human diet, although providing a supplementation of some minerals, particularly Mg and Mn in instant coffees. Additionally, and for authentication purposes, the correlations observed between some elements and the coffee percentage in the blends, with particular significance for Mg amounts, provides a potential tool for the estimation of coffee in substitute blends.     

An approach to calibrate incentives for continuity of supply in the Spanish electricity distribution system

This paper proposes an approach to calibrate incentives for continuity of supply in electricity distribution in Spain. This approach consists on the estimation of the impact of continuity of supply improvements on distribution network costs. For this purpose, distribution costs resulting from different continuity of supply requirements are computed by a reference network model (RNM). The results obtained from the RNM are used to estimate a cost-function that considers continuity of supply a cost driver for distribution costs. This methodology is applied to three Spanish areas of service (an urban one, a semi-urban one and a rural one) in such a way that differences in distribution costs caused by the characteristics of the area type can be taken into account. The analysis indicated that distribution costs are more sensitive to continuity of supply improvements in rural areas of service. It also demonstrated that the incentive for reducing number of service interruptions should be higher than the incentive for reducing duration of service interruptions. Finally, the current Spanish incentive scheme for continuity of supply was analyzed under the perspective of the proposed approach.     

Geotextile filtration opening size under tension and confinement

Nonwoven geotextiles have been used as filters in geotechnical and geoenvironmental works for half a century. They are easy to install and can be specified to meet the requirements for proper filter performance. There are situations where a geotextile filter may be subjected to tensile loads, which may alter relevant filter properties, such as its filtration opening size. Examples of such situations are silty fence applications, geotextile separators, geotextile tubes and geotextiles under embankments on soft soils. This paper investigates the effects of tensile strains on geotextile pore dimensions. A special equipment and testing technique allowed tests to be carried out on geotextile specimens subjected to tension and confinement. The results obtained showed that the variation in filtration opening size depends on the type of strain state the geotextile is subjected, under which the geotextile pore diameter may remain rather constant or increase significantly. However, confinement reduces the geotextile filtration opening size independent on the strain mobilised. An upper bound for the filtration opening size of strained nonwoven geotextiles is introduced and was satisfactory for the geotextile products tested.     

Use of Ozonated Water for Disinfecting Gastrointestinal Endoscopes

The reprocessing of endoscopes is a complex procedure due to their structural design. In the constant search for new antimicrobial substances, recent studies with ozone have yielded great benefits. The present study evaluated the effects of ozonated water used to disinfect endoscopes comparing its efficacy with the conventional technique (2% glutaraldehyde). According to the results obtained, when ozonated water was used (330 mg.min.L^?1), induced a 2 log reduction of the viable microorganisms under the conditions tested. Ozonated water was a potent gastrointestinal endoscopic sanitizer, suggesting it is a feasible alternative for disinfection.     

Improving the performance of fuzzy rules-based forecasters through application of FCM algorithm

Prediction models based on artificial intelligence techniques have been widely used in Time Series Forecasting in several areas. They are often fuzzy models or neural networks. This paper describes the development of neural and fuzzy models for forecasting time series of practical examples, and shows the comparisons of results between models, including the results of statistical modeling. The use of data clustering algorithms like Fuzzy C-Means is considered in fuzzy models.     

Shelf life of powdered Campomanesia adamantium pulp in controlled environments

The objective of this study was to evaluate the shelf life of powdered guavira pulp obtained by a foam mat drying process. The dehydrated guavira pulp was packed into low density polyethylene (LDPE) bags and stored under two controlled conditions: environmental (25 °C, RH 75%) and accelerated (35 °C, RH 90%) for 90 days. The shelf life was accompanied by carrying out the following analyses every 10 days: moisture content, water activity, vitamin C content, pH and titratable acidity. Vitamin C was the quality attribute used to determine the shelf life of the product, by determining its degradation kinetics as a function of storage time. The linear regression data showed that the vitamin C degradation reaction fitted the zero and first order kinetic models. The shelf life of the powdered guavira pulp under environmental conditions was approximately 49 days, and under accelerated conditions (35 °C) 45 days. The Q10 was equal to 1.09, predicting a shelf life similar to that found under environmental conditions. The moisture content for these conditions was 10.0% e 5.4% for 35 °C and 25 °C, respectively. The above demonstrate the efficiency of the accelerated test in predicting the shelf life of the product.