全文获取类型
收费全文 | 1089篇 |
免费 | 120篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 8篇 |
综合类 | 2篇 |
化学工业 | 399篇 |
金属工艺 | 9篇 |
机械仪表 | 44篇 |
建筑科学 | 44篇 |
矿业工程 | 1篇 |
能源动力 | 36篇 |
轻工业 | 332篇 |
水利工程 | 15篇 |
石油天然气 | 8篇 |
无线电 | 28篇 |
一般工业技术 | 144篇 |
冶金工业 | 59篇 |
原子能技术 | 1篇 |
自动化技术 | 80篇 |
出版年
2024年 | 5篇 |
2023年 | 22篇 |
2022年 | 55篇 |
2021年 | 101篇 |
2020年 | 53篇 |
2019年 | 65篇 |
2018年 | 65篇 |
2017年 | 67篇 |
2016年 | 54篇 |
2015年 | 39篇 |
2014年 | 72篇 |
2013年 | 69篇 |
2012年 | 82篇 |
2011年 | 93篇 |
2010年 | 68篇 |
2009年 | 53篇 |
2008年 | 42篇 |
2007年 | 43篇 |
2006年 | 31篇 |
2005年 | 19篇 |
2004年 | 19篇 |
2003年 | 16篇 |
2002年 | 11篇 |
2001年 | 6篇 |
2000年 | 5篇 |
1999年 | 6篇 |
1998年 | 20篇 |
1997年 | 10篇 |
1996年 | 6篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1977年 | 1篇 |
1976年 | 2篇 |
排序方式: 共有1210条查询结果,搜索用时 46 毫秒
61.
The effect of probiotic cultures over Listeria monocytogenes and Escherichia coli O157:H7 during yogurt storage was evaluated. Two different yogurt brands, one with additional probiotic cultures (Lactobacillus casei and L. acidophilus) were inoculated with known populations (106 UFC/g) of either L. monocytogenes or E. coli O157:H7 in three different times and stored for 32 days at 5 degrees C. Every four days the count of lactic bacteria, the added pathogens and pH was evaluated, according to the methodology described in the Bacteriological Analytical Manual. The pH and lactic bacteria population were constant during the testing period. Yogurt with additional probiotic cultures reduced the population of L. monocytogenes in 8 days, the population of E. coli O157:H7 in 16; yogurt with no additional probiotics took 20 days to reduce L. monocytogenes to non-detectable levels and even after 28 days of storage, E. coli O157:H7 was cultured. In this work, the beneficial effects of additional probiotic cultures in yogurt is confirmed again. 相似文献
62.
Luis Gonzlez-Osuna Alfredo Sierra-Cristancho Emilio A. Cafferata Samanta Melgar-Rodríguez Carolina Rojas Paola Carvajal Cristian Cortez Rolando Vernal 《International journal of molecular sciences》2022,23(5)
Senescent cells express a senescence-associated secretory phenotype (SASP) with a pro-inflammatory bias, which contributes to the chronicity of inflammation. During chronic inflammatory diseases, infiltrating CD4+ T lymphocytes can undergo cellular senescence and arrest the surface expression of CD28, have a response biased towards T-helper type-17 (Th17) of immunity, and show a remarkable ability to induce osteoclastogenesis. As a cellular counterpart, T regulatory lymphocytes (Tregs) can also undergo cellular senescence, and CD28− Tregs are able to express an SASP secretome, thus severely altering their immunosuppressive capacities. During periodontitis, the persistent microbial challenge and chronic inflammation favor the induction of cellular senescence. Therefore, senescence of Th17 and Treg lymphocytes could contribute to Th17/Treg imbalance and favor the tooth-supporting alveolar bone loss characteristic of the disease. In the present review, we describe the concept of cellular senescence; particularly, the one produced during chronic inflammation and persistent microbial antigen challenge. In addition, we detail the different markers used to identify senescent cells, proposing those specific to senescent T lymphocytes that can be used for periodontal research purposes. Finally, we discuss the existing literature that allows us to suggest the potential pathogenic role of senescent CD4+CD28− T lymphocytes in periodontitis. 相似文献
63.
Manuel Gacitúa Alexander Carreo Rosaly Morales-Guevara Dayn Pez-Hernndez Jorge I. Martínez-Araya Eyleen Araya Marcelo Preite Carolina Otero María Macarena Rivera-Zaldívar Andrs Silva Juan A. Fuentes 《International journal of molecular sciences》2022,23(5)
Searching for adequate and effective compounds displaying antimicrobial activities, especially against Gram-positive bacteria, is an important research area due to the high hospitalization and mortality rates of these bacterial infections in both the human and veterinary fields. In this work, we explored (E)-4-amino-3-((3,5-di-tert-butyl-2-hydroxybenzylidene)amino) benzoic acid (SB-1, harboring an intramolecular hydrogen bond) and (E)-2-((4-nitrobenzilidene)amino)aniline (SB-2), two Schiff bases derivatives. Results demonstrated that SB-1 showed an antibacterial activity determined by the minimal inhibitory concentration (MIC) against Staphylococcus aureus, Enterococcus faecalis, and Bacillus cereus (Gram-positive bacteria involved in human and animal diseases such as skin infections, pneumonia, diarrheal syndrome, and urinary tract infections, among others), which was similar to that shown by the classical antibiotic chloramphenicol. By contrast, this compound showed no effect against Gram-negative bacteria (Klebsiella pneumoniae, Escherichia coli, and Salmonella enterica). Furthermore, we provide a comprehensive physicochemical and theoretical characterization of SB-1 (as well as several analyses for SB-2), including elemental analysis, ESMS, 1H and 13C NMR (assigned by 1D and 2D techniques), DEPT, UV-Vis, FTIR, and cyclic voltammetry. We also performed a computational study through the DFT theory level, including geometry optimization, TD-DFT, NBO, and global and local reactivity analyses. 相似文献
64.
65.
Carolina Tallon Dorji Chavara Andrew Gillen Daniel Riley Lyndon Edwards Sam Moricca George V. Franks 《Journal of the American Ceramic Society》2013,96(8):2374-2381
Colloidal processing of the Ultra‐High Temperature Ceramic (UHTC) zirconium diboride (ZrB2) to develop near?net‐shaping techniques has been investigated. The use of the colloidal processing technique produces higher particle packing that ultimately enables achieving greater densification at lower temperatures and pressures, even pressureless sintering. ZrB2 suspension formulations have been optimized in terms of rheological behavior. Suspensions were shaped into green bodies (63% relative density) using slip casting. The densification was carried out at 1900°C, 2000°C, and 2100°C, using both hot pressing at 40 MPa and pressureless sintering. The colloidally processed materials were compared with materials prepared by a conventional dry processing route (cold pressed at 50 MPa) and subjected to the same densification procedures. Sintered densities for samples produced by the colloidal route are higher than produced by the dry route (up to 99.5% relative density by hot pressing), even when pressureless sintering is performed (more than 90% relative density). The promising results are considered as a starting point for the fabrication of complex‐shaped components that can be densified at lower sintering temperatures without pressure. 相似文献
66.
Alexander D’ Avignon Flávia Azevedo Carloni Emilio Lèbre La Rovere Carolina Burle Schmidt Dubeux 《Energy Policy》2010
Global concern with climate change has led to the development of a variety of solutions to monitor and reduce emissions on both local and global scales. Under the United Nations Framework Convention on Climate Change (UNFCCC), both developed and emerging countries have assumed responsibility for developing and updating national inventories of greenhouse gas emissions from anthropic sources. This creates opportunities and incentives for cities to carry out their own local inventories and, thereby, develop air quality management plans including both essential key players and stakeholders at the local level. The aim of this paper is to discuss the role of local inventories as an urban public policy instrument and how this type of local instrument may bring advantages countrywide in enhancing the global position of a country. Local inventories have been carried out in many cities of the world and the main advantage of this is that it allows an overview of emissions produced by different municipal activities, thereby, helps decision makers in the elaboration of efficient air quality management plans. In that way, measures aimed at the reduction of fossil fuel consumption to lower local atmospheric pollution levels can also, in some ways, reduce GHG emissions. 相似文献
67.
Antonio Barba Carolina ClausellJuan Carlos Jarque María Monzó 《Journal of the European Ceramic Society》2011,31(12):2119-2128
This paper presents a study of the precipitation of ZnO and CuO crystals during the sintering of Cu-doped Ni-Zn ferrites. The nature of the Zn- and Cu-containing crystal precipitates was determined by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) analysis, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The influence of the dry relative density of the body and the cooling rate on the occurring precipitates was also studied. Two consecutive chemical reactions are put forward, which explain the bulk precipitation of the zinc and copper oxides in the specimen during sintering, and their re-dissolution during cooling. 相似文献
68.
Carolina Merheb-Dini Graziele Aparecida Chiuchi Garcia Ana Lúcia Barretto Penna Eleni Gomes Roberto da Silva 《Food chemistry》2012
Prato cheeses were manufactured using coagulant from Thermomucor indicae-seudaticae N31 or a commercial coagulant. Cheeses were characterised using the following analysis: yield; fat; acidity; moisture; ash; salt; pH; total nitrogen; total protein; NS-pH 4.6/NT*100; NS-TCA 12%/NT*100; casein electrophoresis; and RP-HPLC. The results were statistically analysed and revealed that the proteolytic indices were not significantly different throughout the 60 days of ripening of cheeses made with either coagulant. Even though there were some quantitative differences in the peptide profile of cheeses, the enzyme from T. indicae-seudaticae N31 was used in the production of good quality Prato cheese without having to change the established technological parameters of the process. 相似文献
69.
Tamara Carolina; Timberlake William; Leffel Joseph 《Canadian Metallurgical Quarterly》2010,124(3):302
In two experiments we investigated the extent to which rats (Rattus norvegicus) use an egocentric trajectory and landmarks to locate a goal. In Experiment 1 we trained groups to locate the hidden platform in a water maze with either 1 of 3 or 3 of 3 predictive landmarks, and with either a random or fixed egocentric trajectory. A choice test revealed that regardless of the landmark configuration, rats relied on a directional, egocentric trajectory, when it was available, to locate the platform. In Experiment 2 we found that adding four predictive landmarks following training with a constant egocentric trajectory did not alter rats' initial attention to the trajectory. We conclude that the presence of nonpredictive landmarks in a predictive array did not affect the use of landmarks. With a blocking design, rats used initially an egocentric path, then landmarks. These results add to the notion that animals use available spatial cues sequentially. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
70.
Isabel Comino Ana Real Maria de Lourdes Moreno Raquel Montes Ángel Cebolla Carolina Sousa 《Journal of the science of food and agriculture》2013,93(4):933-943
BACKGROUND: Cereals used for beer manufacturing contain gluten, which is immunotoxic for celiac patients. The gluten remaining after processes of malting and brewing is mostly hydrolyzed, which makes practical evaluation of the immunotoxicity of the gluten pools challenging. RESULTS: We analyzed the presence of gluten peptides equivalent to the major immunotoxic protease‐resistant gliadin 33‐mer in 100 Belgium beers, using monoclonal antibodies (G12/A1). Immunochromatographic strips and enzyme‐linked immonosorbent assay G12/A1 methods estimated at least 20 ppm gluten equivalents in 90 beers and gluten‐free in 10 beers. The G12/A1 reactivity of beer high‐performance liquid chromatographic fractions correlated to the presence of T‐cell‐reactive epitopes identified by peptide sequencing. CONCLUSION: The determination of equivalent gliadin 33‐mer epitopes in beers has been shown to be practical, specific, and sensitive for the measurement of potential immunotoxicity for celiac patients. Copyright © 2012 Society of Chemical Industry 相似文献