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81.
Laser-induced damage in bare glass substrates and thin films has long been widely acknowledged as a localized phenomenon associated with the presence of micrometer and submicrometer scale defects. The scanning of both optical absorption and scattering allows us to discriminate between absorbing and nonabsorbing defects and can give specific information about the origin of the defects. We investigate the spectral properties of defects in thin films and fused-silica surfaces. Absorbing and scattering defects are studied at different wavelengths in the ultraviolet, visible, and infrared ranges. Absorbing defects are shown to be highly wavelength dependent, whereas we have observed significant correlation between scattering defects. 相似文献
82.
High-throughput method for the production and analysis of large natural product libraries for drug discovery 总被引:6,自引:0,他引:6
Eldridge GR Vervoort HC Lee CM Cremin PA Williams CT Hart SM Goering MG O'Neil-Johnson M Zeng L 《Analytical chemistry》2002,74(16):3963-3971
High-throughput methods were applied to the production, analysis, and characterization of libraries of natural products in order to accelerate the drug discovery process for high-throughput screening in the pharmaceutical and biotechnology industries. Library production integrates automated flash chromatography, solid-phase extraction, filtration, and high-throughput parallel four-channel preparative high-performance liquid chromatography to obtain the libraries in 96- or 384-well plates. Libraries consist of purified fractions with approximately one to five compounds per well. Libraries are analyzed prior to biological screening by a high-throughput parallel eight-channel liquid chromatography-evaporative light scattering detection-mass spectrometry system to determine the molecular weight, number, and quantity of compounds in a fraction. After biological screening, active fractions are rapidly purified at the microgram level and individual compounds are rescreened for confirmation of activity. Structures of active compounds are elucidated by NMR spectroscopy and mass spectrometry. Utilization of a novel microcoil probe allows NMR data to be gathered on 50 microg. As a demonstration, a library was made from the stem bark of Taxus brevifolia. Biological screening in the National Cancer Institute's in vitro panel of three cancer cell lines demonstrates that the process enables the discovery of active anticancer compounds not detected in the flash fractions from which the library originates. 相似文献
83.
Caroline Seck Langill 《AI & Society》2006,20(1):49-62
Over the past decade artists have increasingly turned to science in order to investigate technology’s effect. The move from
hardware-based technologies to live organisms as media, raises ethical issues that the broader art community is addressing.
This paper tracks the history of instrumental disengagement to determine when and how the gradual codification of life contributed
to the eventual use of live organisms in art practice. 相似文献
84.
R Penagini G Hebbard M Horowitz J Dent H Bermingham K Jones RH Holloway 《Canadian Metallurgical Quarterly》1998,42(2):251-257
Oxytocin (OT) is present in the mammalian testis and has been shown to play a role in the modulation of seminiferous tubule contractility and steroidogenesis. However, stage-specific effects of the peptide have not been previously investigated. In this study, computer-assisted analysis and time-lapse videomicrography were used to investigate basal contractility and the response to OT of seminiferous tubules at specific stages of the spermatogenic cycle. Adult rat testes were placed in fresh oxygenated DMEM F12 medium, decapsulated, and the tubules gently teased apart. Stages were identified by transillumination and a 10 mm section of tubule at each of stages IV-V, VII-VIII and XIII-I was placed in a microslide chamber and perifused with medium. After a control period of 3 h, OT (2 nM) was given for 1 h, followed by another control period of 1 h. The experiment was repeated using tubules from different rats and data were analysed to give arbitrary units of tubule contractility. Contractility was observed in all the tubules studied and the contractile activity was shown to vary depending on the stage of the spermatogenic cycle. Mean basal contractility at stages VII-VIII, the time when sperm are shed from the epithelium, was significantly lower than that at stages IV-V and XIII-I. The response of the tubules to OT was also stage-dependent, with the peptide producing the largest increases in contractile activity at stages VII-VIII and having no effect at stages IV-V. We postulate that these stage-specific differences in basal and OT-stimulated contractility may be important in co-ordinating the movement of developing germ cells towards the lumen of the seminiferous epithelium and in the process of spermiation. 相似文献
85.
86.
Le Roux B Conan JM Kulcsár C Raynaud HF Mugnier LM Fusco T 《Journal of the Optical Society of America. A, Optics, image science, and vision》2004,21(7):1261-1276
Classical adaptive optics (AO) is now a widespread technique for high-resolution imaging with astronomical ground-based telescopes. It generally uses simple and efficient control algorithms. Multiconjugate adaptive optics (MCAO) is a more recent and very promising technique that should extend the corrected field of view. This technique has not yet been experimentally validated, but simulations already show its high potential. The importance for MCAO of an optimal reconstruction using turbulence spatial statistics has already been demonstrated through open-loop simulations. We propose an optimal closed-loop control law that accounts for both spatial and temporal statistics. The prior information on the turbulence, as well as on the wave-front sensing noise, is expressed in a state-space model. The optimal phase estimation is then given by a Kalman filter. The equations describing the system are given and the underlying assumptions explained. The control law is then derived. The gain brought by this approach is demonstrated through MCAO numerical simulations representative of astronomical observation on a 8-m-class telescope in the near infrared. We also discuss the application of this control approach to classical AO. Even in classical AO, the technique could be relevant especially for future extreme AO systems. 相似文献
87.
88.
Lisa Stead Petar Goulev Caroline Evans Ebrahim Mamdani 《Personal and Ubiquitous Computing》2004,8(3-4):282-290
Since the industrial revolution, fashion and technology have been linked through the textile and manufacturing industries, a relationship that has propelled technical innovation and aesthetic and social change. Today, a new alliance is emerging through the integration of electronic technology and smart materials on the body. This study addresses the integration of technology with clothing from a fashion perspective, and examines its expressive and interactive potential. It proposes the concept of The Emotional Wardrobe: clothing that represents and stimulates emotional response through the interface of technology. It asks if fashion can offer a more personal and provocative definition of self, which actively involves the wearer in a mutable aesthetic identity. A multi-disciplinary framework combines fashion, material science and the real-time, affective computing platform, called AffectiveWare. By merging technology and fashion, The Emotional Wardrobe becomes a poetic interface, shifting emphasis from human–computer interaction to computer-aided, human–human communication. 相似文献
89.
90.