全文获取类型
收费全文 | 358篇 |
免费 | 24篇 |
专业分类
电工技术 | 3篇 |
化学工业 | 166篇 |
金属工艺 | 1篇 |
机械仪表 | 9篇 |
建筑科学 | 13篇 |
能源动力 | 6篇 |
轻工业 | 53篇 |
水利工程 | 2篇 |
石油天然气 | 1篇 |
无线电 | 13篇 |
一般工业技术 | 66篇 |
冶金工业 | 19篇 |
自动化技术 | 30篇 |
出版年
2024年 | 1篇 |
2023年 | 9篇 |
2022年 | 53篇 |
2021年 | 45篇 |
2020年 | 10篇 |
2019年 | 13篇 |
2018年 | 11篇 |
2017年 | 15篇 |
2016年 | 11篇 |
2015年 | 7篇 |
2014年 | 20篇 |
2013年 | 9篇 |
2012年 | 24篇 |
2011年 | 21篇 |
2010年 | 20篇 |
2009年 | 17篇 |
2008年 | 14篇 |
2007年 | 12篇 |
2006年 | 13篇 |
2005年 | 7篇 |
2004年 | 7篇 |
2003年 | 4篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1999年 | 4篇 |
1998年 | 6篇 |
1997年 | 4篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1991年 | 2篇 |
1989年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1980年 | 1篇 |
排序方式: 共有382条查询结果,搜索用时 15 毫秒
41.
Andrea Angius Antonio Mario Scanu Caterina Arru Maria Rosaria Muroni Vincenzo Rallo Giulia Deiana Maria Chiara Ninniri Ciriaco Carru Alberto Porcu Giovanna Pira Paolo Uva Paolo Cossu-Rocca Maria Rosaria De Miglio 《International journal of molecular sciences》2021,22(4)
Colorectal cancer (CRC) is a leading cause of cancer death worldwide, and about 20% is metastatic at diagnosis and untreatable. Increasing evidence suggests that the heterogeneous nature of CRC is related to colorectal cancer stem cells (CCSCs), a small cells population with stemness behaviors and responsible for tumor progression, recurrence, and therapy resistance. Growing knowledge of stem cells (SCs) biology has rapidly improved uncovering the molecular mechanisms and possible crosstalk/feedback loops between signaling pathways that directly influence intestinal homeostasis and tumorigenesis. The generation of CCSCs is probably connected to genetic changes in members of signaling pathways, which control self-renewal and pluripotency in SCs and then establish function and phenotype of CCSCs. Particularly, various deregulated CCSC-related miRNAs have been reported to modulate stemness features, controlling CCSCs functions such as regulation of cell cycle genes expression, epithelial-mesenchymal transition, metastasization, and drug-resistance mechanisms. Primarily, CCSC-related miRNAs work by regulating mainly signal pathways known to be involved in CCSCs biology. This review intends to summarize the epigenetic findings linked to miRNAome in the maintenance and regulation of CCSCs, including their relationships with different signaling pathways, which should help to identify specific diagnostic, prognostic, and predictive biomarkers for CRC, but also develop innovative CCSCs-targeted therapies. 相似文献
42.
Carina A. Lämmle Adam Varady Thorsten G. Müller Dr. Caterina Sturtzel Michael Riepl Bettina Mathes Jenny Eichhorst Dr. Anje Sporbert Dr. Martin Lehmann Prof. Dr. Hans-Georg Kräusslich Dr. Martin Distel Dr. Johannes Broichhagen 《Chembiochem : a European journal of chemical biology》2021,22(3):548-556
Selective targeting of DNA by means of fluorescent labeling has become a mainstay in the life sciences. While genetic engineering serves as a powerful technique and allows the visualization of nucleic acid by using DNA-targeting fluorescent fusion proteins in a cell-type- and subcellular-specific manner, it relies on the introduction of foreign genes. On the other hand, DNA-binding small fluorescent molecules can be used without genetic engineering, but they are not spatially restricted. Herein, we report a photocaged version of the DNA dye Hoechst33342 (pcHoechst), which can be uncaged by using UV to blue light for the selective staining of chromosomal DNA in subnuclear regions of live cells. Expanding its application to a vertebrate model organism, we demonstrate uncaging in epithelial cells and short-term cell tracking in vivo in zebrafish. We envision pcHoechst as a valuable tool for targeting and interrogating DNA with precise spatiotemporal resolution in living cells and wild-type organisms. 相似文献
43.
Alessandro Caputo Angela DArdia Francesco Sabbatino Caterina Picariello Chiara Ciaparrone Pio Zeppa Antonio DAntonio 《International journal of molecular sciences》2021,22(9)
The current standard of care for advanced non-small-cell lung cancer is based on detecting actionable mutations that can benefit from targeted therapy. Comprehensive genetic tests can have long turn-around times, and because EGFR mutations are the most prevalent actionable mutation, a quick detection would enable a prompt initiation of targeted therapy. Furthermore, the scarcity of diagnostic material means that sometimes only cytologic material is available. The Idylla™ EGFR assay is a real-time PCR–based method able to detect 51 EGFR mutations in 2.5 h. Idylla is validated for use only on FFPE sections, but some researchers described their experiences with cytological material. We reviewed the relevant literature, finding four articles describing 471 cases and many types of cytological input material: smears, cell-block sections, suspensions, and extracted DNA. The sensitivity, specificity, and limit of detection appear comparable to those obtained with histological input material, with one exception: the usage of scraped stained smears as input may reduce the accuracy of the test. In conclusion, usage of cytological material as input to the Idylla EGFR test is possible. A workflow where common mutations are tested first and fast, leaving rarer mutations for subsequent comprehensive profiling, seems the most effective approach. 相似文献
44.
Buzzi Maria Claudia Buzzi Marina Perrone Erico Senette Caterina 《Universal Access in the Information Society》2019,18(4):891-907
Universal Access in the Information Society - Today, ICT technology offers mobile, customized user-friendly environments to support learning and stimulate an individual’s potential. However,... 相似文献
45.
46.
Carone Simone Moramarco Vincenzo Pappalettera Giovanni Barbieri Giuseppe Casavola Caterina 《Journal of Materials Science》2022,57(1):671-686
Journal of Materials Science - Residual stress assessment is a key factor in engineering design owing to its impact on engineering properties of materials, structural components and welded joints.... 相似文献
47.
Fan Fei Parth Kotak Li He Xiaofeng Li Cyan Vanderhoef Caterina Lamuta Xuan Song 《Advanced functional materials》2021,31(46):2105528
Cutaneous muscles drive the texture-modulation behavior of cephalopods by protruding several millimeters out of the skin. Inspired by cephalopods, a self-morphing, stretchable smart skin containing embedded-printed electrodes and actuated by Twisted Spiral Artificial Muscles (TSAMs) is proposed. Electrothermally actuated TSAMs are manufactured from inexpensive polymer fibers to mimic the papillae muscles of cephalopods. These spirals can produce strains of nearly 2000% using a voltage of only 0.02 V mm−1. Stretchable and low-resistance liquid metal electrodes are embedded-printed inside the self-morphing skin to facilitate the electrothermal actuation of TSAMs. Theoretical and numerical models are proposed to describe the embedded printing of low-viscosity Newtonian liquid metals as conductive electrodes in a soft elastomeric substrate. Experimental mechanical tests are performed to demonstrate the robustness and electrical stability of the electrodes. Two smart skin prototypes are fabricated to highlight the capabilities of the proposed self-morphing system, including a texture-modulating wearable soft glove and a waterproof skin that emulates the texture-modulation behavior of octopi underwater. The proposed self-morphing stretchable smart skin can find use in a wide range of applications, such as refreshable Braille displays, haptic feedback devices, turbulence tripping, and antifouling devices for underwater vehicles. 相似文献
48.
We explore the use of radial basis functions (RBF) in the weighted essentially non-oscillatory (WENO) reconstruction process used to solve hyperbolic conservation laws, resulting in a numerical method of arbitrarily high order to solve problems with discontinuous solutions. Thanks to the mesh-less property of the RBFs, the method is suitable for non-uniform grids and mesh adaptation. We focus on multiquadric radial basis functions and propose a simple strategy to choose the shape parameter to control the balance between achievable accuracy and the numerical stability. We also develop an original smoothness indicator which is independent of the RBF for the WENO reconstruction step. Moreover, we introduce type I and type II RBF-WENO methods by computing specific linear weights. The RBF-WENO method is used to solve linear and nonlinear problems for both scalar and systems of conservation laws, including Burgers equation, the Buckley–Leverett equation, and the Euler equations. Numerical results confirm the performance of the proposed method. We finally consider an effective conservative adaptive algorithm that captures moving shocks and rapidly varying solutions well. Numerical results on moving grids are presented for both Burgers equation and the more complex Euler equations. 相似文献
49.
Darton NJ Sederman AJ Ionescu A Ducati C Darton RC Gladden LF Slater NK 《Nanotechnology》2008,19(39):395102
The use of magnetic fields in magnetic resonance imaging (MRI) for the tracking and delivery of chemotherapeutics bound to superparamagnetic nanoparticles offers a promising method for the non-invasive treatment of inoperable tumours. Here we demonstrate that superparamagnetic magnetite nanoparticles fabricated by an easily scalable method can be driven and tracked in real time at high velocities in vitro using MRI hardware. Force balance calculations are consistent with the magnetic properties of individual 10 nm diameter particles that move collectively as micron sized agglomerates with hydrodynamic diameter similar to that inferred from zero-magnetic-field dynamic light scattering measurements. 相似文献
50.
Li‐S‐Batteries: Advanced Lithium–Sulfur Batteries Enabled by a Bio‐Inspired Polysulfide Adsorptive Brush (Adv. Funct. Mater. 46/2016)
下载免费PDF全文
![点击此处可从《Advanced functional materials》网站下载免费的PDF全文](/ch/ext_images/free.gif)