Microscopic diagnosis of the leaf and stem of Piper solmsianum C.DC

Piper solmsianum C.DC., which is popularly known as pariparoba, is a shrub that measures 1–3 m in height and it inhabits areas with wet tropical soils. The objective of this study was to analyze the leaf and stem anatomy using light microscopy, scanning electron micrographs, and energy‐dispersive X‐ray spectroscopy in order to provide information for species identification. The anatomical profile showed the following main microscopic markers: hypostomatic leaf; hypodermis layer on both sides; pearl glands; biconvex midrib shape; five collateral vascular bundles in open arc with the central bundle larger than the others; circular stem shape; collateral vascular bundles arranged in two rings; sinuous sclerenchymatic sheath in the pith; secretory idioblasts; and starch grains in the mesophyll, in the ground parenchyma of the midrib, petiole, and in the stem; and six morphotypes of calcium oxalate crystals (styloids, cuneiform, tabular crystal rosettes, cuneiform crystal rosettes, elongated square dipyramids, as well as very elongated square dipyramids).     

Novel Tacrine‐Grafted Ugi Adducts as Multipotent Anti‐Alzheimer Drugs: A Synthetic Renewal in Tacrine–Ferulic Acid Hybrids

Herein we describe the design, multicomponent synthesis, and biological, molecular modeling and ADMET studies, as well as in vitro PAMPA‐blood–brain barrier (BBB) analysis of new tacrine–ferulic acid hybrids (TFAHs). We identified (E)‐3‐(hydroxy‐3‐methoxyphenyl)‐N‐{8[(7‐methoxy‐1,2,3,4‐tetrahydroacridin‐9‐yl)amino]octyl}‐N‐[2‐(naphthalen‐2‐ylamino)2‐oxoethyl]acrylamide (TFAH 10 n ) as a particularly interesting multipotent compound that shows moderate and completely selective inhibition of human butyrylcholinesterase (IC₅₀=68.2 nM ), strong antioxidant activity (4.29 equiv trolox in an oxygen radical absorbance capacity (ORAC) assay), and good β‐amyloid (Aβ) anti‐aggregation properties (65.6 % at 1:1 ratio); moreover, it is able to permeate central nervous system (CNS) tissues, as determined by PAMPA‐BBB assay. Notably, even when tested at very high concentrations, TFAH 10 n easily surpasses the other TFAHs in hepatotoxicity profiling (59.4 % cell viability at 1000 μM ), affording good neuroprotection against toxic insults such as Aβ_1–40, Aβ_1–42, H₂O₂, and oligomycin A/rotenone on SH‐SY5Y cells, at 1 μM . The results reported herein support the development of new multipotent TFAH derivatives as potential drugs for the treatment of Alzheimer′s disease.     

Potent,Metabolically Stable 2‐Alkyl‐8‐(2H‐1,2,3‐triazol‐2‐yl)‐9H‐adenines as Adenosine A2A Receptor Ligands

Inhibition of adenosine A_2A receptors has been shown to elicit a therapeutic response in preclinical animal models of Parkinson’s disease (PD). We previously identified the triazolo‐9H‐purine, ST1535, as a potent A_2AR antagonist. Studies revealed that ST1535 is extensively hydroxylated at the ω‐1 position of the butyl side chain. Here, we describe the synthesis and evaluation of derivatives in which the ω‐1 position has been substituted (F, Me, OH) in order to block metabolism. The stability of the compounds was evaluated in human liver microsomes (HLM), and the affinity for A_2AR was determined. Two compounds, (2‐(3,3‐dimethylbutyl)‐9‐methyl‐8‐(2H‐1,2,3‐triazol‐2‐yl)‐9H‐purin‐6‐amine ( 3 b ) and 4‐(6‐amino‐9‐methyl‐8‐(2H‐1,2,3‐triazol‐2‐yl)‐9H‐purin‐2‐yl)‐2‐methylbutan‐2‐ol ( 3 c ), exhibited good affinity against A_2AR (K_i=0.4 nM and 2 nM , respectively) and high in vitro metabolic stability (89.5 % and 95.3 % recovery, respectively, after incubation with HLM for two hours).     

Neutronic characterization of the MEGAPIE target

The MEGAPIE project aimed to design, build and operate a liquid metal spallation neutron target of about 1 MW beam power in the SINQ facility at the Paul Scherrer Institut (Villigen, Switzerland). This project is an important step in the roadmap towards the demonstration of the accelerator driven system (ADS) concept and high power liquid metal targets in general. Following the design phase, an experimental program was defined to provide a complete characterization of the facility by performing a “mapping” of the neutron flux at different points, from the center of the target to the beam lines. The neutronic performance of the target was studied using different experimental techniques with the goals of validating the Monte Carlo codes used in the design of the target; additionally, the performance was compared with the solid lead targets used before and after the MEGAPIE experiment.     

Effect of finishing and ageing time on quality attributes of loin from the meat of Holstein–Fresian cull cows

The effects of finishing time, (T0 = 0, T1 = 30 and T2 = 60 days), on Holstein–Friesian cull cows (n = 18) and post-mortem ageing, (1, 7, 14, 21, 35 and 42 days), under vacuum conditions of Longissimus thoracis (LT) muscles were investigated. The objective of this research was to study how finishing feeding (based on a commercial concentrate and corn silage), following a pasture period of 90 days, affected carcass and meat quality. Ageing time effect was also evaluated on the main quality attribute of added value pieces, such as “striploin of ox” from cull cows. Finishing treatment affected intramuscular fat content (IMF), moisture percentage, water-holding capacity (WHC), colour parameters and shear force of meat at 24 h post-mortem, whereas ageing time enhanced meat tenderness, when this was measured by two textural tests, Warner–Braztler (WB) and textural profile analysis (TPA). A minimum shear force was achieved at 7 and 14 days of ageing for T1 and T2, respectively. No differences (P > 0.05) could be found in colour parameters from 7 to 42 days. The results show that a finishing time of two months is very beneficial, due to the increase in meat fatness, improved overall carcass quality and luminosity (L*). Furthermore, 14 ageing days were sufficient to improved tenderness. Ageing time did not have an effect on lipid oxidation (P > 0.05) and this leads us to conclude that meat shelf life exceeded 42 days under vacuum conditions’.     

Lc/Esi/Ms/Ms determination of postharvest fungicide residues in citrus juices

An LC-ESI-MS/MS method was developed for the quantitative detection of postharvest fungicide residues in citrus juices and reported in this paper. The analyses of thiabendazole (TBZ), carbendazim (MBC), thiophanate methyl (TPM), imazalil (IMZ) and prochloraz (PCZ) residues were performed by using a gradient elution in conjunction with positive ionization mode electrospray ionization tandem mass spectrometry. Fungicides were extracted from citrus juices with recoveries ranging from 79.8 to 101.2% and relative standard deviation better than 15%. The quantification limits ranged from 0.01 μg/kg IMZ to 0.06 μg/kg for MBC. The LC-MS-MS method was applied to commercial citrus juices, detecting MBC, TBZ and IMZ residues in the 90% of the samples. Prochloraz residues were detected only in one of the multifruit juice (orange, lemon and carrot) samples.     

The influence of nitrogen incorporation on the optical properties of anodic Ta2O5

Anodic oxides were grown on sputter-deposited Ta in different aqueous solutions. A photoelectrochemical investigation was performed in order to estimate the band gap of the films as a function of the anodizing bath composition and formation voltage, i.e. thickness. Photoelectrochemical results provided evidence of sub-band gap photocurrent for films formed in a bath containing ammonium ions at pH 9. Elemental depth profiles obtained by glow discharge optical emission spectroscopy revealed the presence of nitrogen species in the outer part of the anodic films, which is bonded to Ta according to XPS analysis. A mechanism of nitrogen incorporation is proposed in order to account for the pH dependence of film composition.     

Studies on the Susceptibility of Granular Cassava and Corn Starches to Enzymatic Attack. Part 2: Study of the Granular Structure of Starch

The starch granules remaining after enzymatic hydrolysis were analyzed. Enzymatic corrosion efficiency was related to granule diameter. The intrinsic viscosity of enzymatically treated cassava and corn starches was gradually lowered by hydrolysis. X-ray analysis and observation under a polarizing microscope suggested that enzymatic corrosion must occur in amorphous areas of the granules. Scanning electron microscopy showed that, for cassava granules, enzymatic corrosion occured mainly at the surface. Corn starch granules displayed considerable surface corrosion, as well as deep radial corrosion channels.     

Silicon planar technology for single-photon optical detectors

Design and fabrication of single photon avalanche detector (SPAD) in planar technology is reported. Device design and critical issues in the technology are discussed. Experimental test procedures are described for dark-counting rate, afterpulsing probability, photon timing resolution, and quantum detection efficiency. Low-noise detectors are obtained, with dark counting rates down to 10 c/s for devices with 10 /spl mu/m diameter, down to 1 kc/s for 50 /spl mu/m diameter. The technology is suitable for monolithic integration of SPAD detectors and associated circuits.     

Pipeline of Successive Approximation Converters with Optimum Power Merit Factor

In this paper, we present a low power 12 bit 5 MSPS, successive approximation converter architecture using pipeline technique. The converter consumes 4 mW at the Nyquist rate input with 1.8 V power supply. By combination of pipeline and successive architecture, the entire circuit, simulated at the transistor level in a 0.18 μ CMOS process, achieves a FoM (Figure of Merit) of 0.19 pJ/conversion. Jinghua Li was born in 1973. He received the MSEE and BSEE Degree from College of Electronics and information, Shanghai Jiaotong University and Harbin Engineering University in 1997 and 1994 respectively. He is currently pursuing Ph.D degree in Department of Electrical Engineering, Texas A&M University, College Station, TX, USA. In 1997, he joined Bell Laboratory (China), Lucent Technologies as a member of technical staff. He worked on single-chip HDTV decoder IC and Sonet/SDH SoC for various projects in Murray Hill, NJ, USA and Shanghai China. He also finished projects on hardware implementation of Video conference/Phone based on H.263 standard as his master thesis. Since 2000, he has been a research assistant in Analog Mixed Signal center, TAMU. Most currently his research interests are focused on low power analog to digital conversion IC design, CMOS implementation of 10 G/2.5 G clock data recovery IC for high speed serial communications. Franco Maloberti received the Laurea Degree in Physics (Summa cum Laude) from the University of Parma, Parma Italy, in 1968 and the Dr. Honoris Causa degree in electronics from the Instituto Nacional de Astrofisica, Optica y Electronica (Inaoe), Puebla, Mexico in 1996. In 1993 he was a Visiting Professor at ETH-PEL, Zurich. He was Professor of Microelectronics and Head of the Micro Integrated Systems Group University of Pavia, Pavia, Italy and the TI/J.Kilby Analog Engineering Chair Professor at the Texas A&M University. He is currently the Distinguished Microelectronic Chair Professor at University of Texas at Dallas and part-time Professor at the University of Pavia, Italy. His professional expertise is in the design, analysis and characterization of integrated circuits and analogue digital applications, mainly in the areas of switched capacitor circuits, data converters, interfaces for telecommunication and sensor systems, and CAD for analogue and mixed A-D design. He has written more than 250 published papers, three books and holds 15 patents. He was in 1992 recipient of the XII Pedriali Prize for his technical and scientific contributions to national industrial production. He was co-recipient of the 1996 Institute of Electrical Engineers (U.K.) Fleming Premium for the paper “CMOS Triode Transistor Transconductor for high-frequency continuous time filters.” He has been responsible at both technical and management levels for many research programs including ten ESPRIT projects and has served the European Commission as ESPRIT Projects' Evaluator, Reviewer and as European Union expert in many European Initiatives. He served the Academy of Finland on the assessment of electronic research in Academic institutions and on the research programs' evaluations. Dr. Maloberti was Vice-President, Region 8, of the IEEE Circuit and Systems Society from 1995 to 1997 and an Associate Editor of IEEE-Transaction on Circuit and System-II. He received the 1999 IEEE CAS Society Meritorious Service Award, the 2000 CAS Society Golden Jubilee Medal, and the IEEE Millenium Medal. He is the President of the IEEE Sensor Council and member of the Board of Governors of the IEEE CAS Society. He is a member of the Italian Electrothecnical and Electronic Society (AEI), the Editorial Board of Analog Integrated Circuits and Signal Processing, and Fellow of IEEE.