Repetitive Sequence Barcode Probe for Karyotype Analysis in Tripidium arundinaceum

The barcode probe is a convenient and efficient tool for molecular cytogenetics. Tripidium arundinaceum, as a polyploid wild allied genus of Saccharum, is a useful genetic resource that confers biotic and abiotic stress resistance for sugarcane breeding. Unfortunately, the basic cytogenetic information is still unclear due to the complex genome. We constructed the Cot-20 library for screening moderately and highly repetitive sequences from T. arundinaceum, and the chromosomal distribution of these repetitive sequences was explored. We used the barcode of repetitive sequence probes to distinguish the ten chromosome types of T. arundinaceum by fluorescence in situ hybridization (FISH) with Ea-0907, Ea-0098, and 45S rDNA. Furthermore, the distinction among homology chromosomes based on repetitive sequences was constructed in T. arundinaceum by the repeated FISH using the barcode probes including Ea-0663, Ea-0267, EaCent, 5S rDNA, Ea-0265, Ea-0070, and 45S rDNA. We combined these probes to distinguish 37 different chromosome types, suggesting that the repetitive sequences may have different distributions on homologous chromosomes of T. arundinaceum. In summary, this method provide a basis for the development of similar applications for cytogenetic analysis in other species.     

Maternal Prenatal Inflammation Increases Brain Damage Susceptibility of Lipopolysaccharide in Adult Rat Offspring via COX-2/PGD-2/DPs Pathway Activation

A growing body of research suggests that inflammatory insult contributes to the etiology of central nervous system diseases, such as depression, Alzheimer’s disease, and so forth. However, the effect of prenatal systemic inflammation exposure on offspring brain development and cerebral susceptibility to inflammatory insult remains unknown. In this study, we utilized the prenatal inflammatory insult model in vivo and the neuronal damage model in vitro. The results obtained show that prenatal maternal inflammation exacerbates LPS-induced memory impairment, neuronal necrosis, brain inflammatory response, and significantly increases protein expressions of COX-2, DP2, APP, and Aβ, while obviously decreasing that of DP1 and the exploratory behaviors of offspring rats. Meloxicam significantly inhibited memory impairment, neuronal necrosis, oxidative stress, and inflammatory response, and down-regulated the expressions of APP, Aβ, COX-2, and DP2, whereas significantly increased exploring behaviors and the expression of DP1 in vivo. Collectively, these findings suggested that maternal inflammation could cause offspring suffering from inflammatory and behavioral disorders and increase the susceptibility of offspring to cerebral pathological factors, accompanied by COX-2/PGD-2/DPs pathway activation, which could be ameliorated significantly by COX-2 inhibitor meloxicam treatment.     

Peptide Assembly of Al/CuO Nanothermite for Enhanced Reactivity of Nanoaluminum Particles

Biological self-assembly procedures, which are generally carried out in an aqueous solution, have been found to be the most promising method for directing the fabrication of diverse nanothermites, including Al/CuO nanothermite. However, the aqueous environment in which Al nanoparticles self-assemble has an impact on their stability. We show that using a peptide to self-assemble Al or CuO nanoparticles considerably improves their durability in phosphate buffer aqueous solution, with Al and CuO nanoparticles remaining intact in aqueous solution for over 2 weeks with minimal changes in the structure. When peptide-assembled Al/CuO nanothermite was compared with a physically mixed sample in phosphate buffer for 30 min, the energy release of the former was higher by 26%. Furthermore, the energy release of peptide-assembled Al/CuO nanocomposite in phosphate buffer showed a 6% reduction by Day 7, while that of the peptide-assembled Al/CuO nanocomposite in ultrapure water was reduced by 75%. Taken together, our study provides an easy method for keeping the thermal activity of Al/CuO nanothermite assembled in aqueous solution.     

一种空空导弹发射装置故障诊断仪设计

导弹发射装置是目前我国某型战斗机所装备的主要作战武器之一,它的性能直接影响到战斗机的战斗能力,由于目前缺乏有效的故障诊断系统和仪器对导弹发射装置进行测试,所以设计了一种空空导弹发射装置故障诊断仪器;该仪器以工业控制计算机为核心,采用数据库编程技术编程,综合运用标准接口及总线技术、故障诊断技术,FIFO技术,有效地提高了仪器的通用性、可靠性;研究结果表明,该仪器能够对某型空空导弹发射装置的各种故障进行快速准确的诊断,提高部队的保障能力,具有很好的推广应用前景和重大的经济效益.     

Strengthening and toughening mechanism of a Cu-bearing high-strength low-alloy steel with refined tempered martensite/bainite (M/B) matrix and minor inter-critical ferrite

The microstructure–mechanical property relationship of a Cu-bearing low-carbon high-strength low-alloy steel, subjected to a novel multistage heat treatment inc...     

光子晶体光纤飞秒激光技术研究进展

光子晶体光纤自诞生至今的十几年来得到了快速发展,不同结构和各具特色的光子晶体光纤层出不穷。以应用于飞秒激光技术的各种光子晶体光纤为主线,介绍了目前基于光子晶体光纤飞秒激光技术的实验研究进展,尤其是高功率、高能量飞秒激光系统的研究现状和发展方向。     

Linear Study of a Large-orbit Coaxial-waveguide Cyclotron Autoresonance Maser Amplifier

A linear theory based on Maxwell-Vlasov equations and the Laplace transform is presented to describe the large-orbit coaxial-waveguide cyclotron autoresonance maser (CARM) amplifier. A 35-GHz, TE_5,1-mode large-orbit coaxial-waveguide CARM amplifier is specifically analyzed. Good agreement between the linear theory and the nonlinear simulations is found in the exponentially growing region of the wave. Results show that the operating magnetic field and the electron-beam energy and average radius substantially affect the power, and especially, a small beam current may be unsuitable to a large-orbit CARM amplifier operation.     

小波变换的基本原理及其在混凝土检测中的应用

小波分析在实域和频域同时具有良好的局部化特性,将其引入到桥梁混凝土结构的无损检测中,对超声接收信号进行多分辨率分析,是结构损伤识别的有效方法。     

带闪蒸器补气的R134a准二级压缩制冷/热泵系统实验研究

为增加空气源热泵运行的稳定性及提高其性能系数,本文提出了以R134a为工质的涡旋压缩机闪蒸器补气制冷/热泵系统。搭建了实验台对压缩机排气温度、功耗、制冷量、制热量及制冷、制热性能系数进行研究。结果表明:当冷凝温度为45℃,蒸发温度为-20~0℃时,与采用相同工质的单级系统相比,补气系统的排气温度降低了6.2℃,功耗增加1.4%~2.8%,制冷量和制冷COPc分别提高19.8%和17.6%,制热量和制热COPh分别提高15.3%和13.2%。     

麦秸秆加筋盐渍土的尺寸效应与制样问题处置

制备均匀且整体性较好的试样是保证室内实验结果可靠的前提条件。提出了两端压实的静力制样法,围绕制样中出现的几个问题,制定了适宜的应对措施。同时完成了不同直径的盐渍土和麦秸秆加筋土试样的抗压强度实验,分析了试样抗压强度的尺寸效应,以及试样直径与加筋条件间的比例关系。结果显示:采用两端压实的静力制样方法制备加筋土试样是可行的;两种试样均存在明显的尺寸效应;由于麦秸秆的加筋作用,使得麦秸秆加筋土不同尺寸试样的抗压强度尺寸效应大于盐渍土的,且其抗压强度的尺寸效应随试样尺寸的增大而越来越明显;适宜加筋长度为0.316倍试样直径,适宜加筋率0.25%。研究结果可为制备合格的加筋土试样提供参考,也有益于在未来的研究中选择更合理的麦秸秆加筋条件。