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91.
Bovine pancreatic lipase was isolated in pure form by lyophilization of fresh bovine pancreas, extraction of the enzyme with sucrose solution, fractional precipitation with ammonium sulfate and acetone, followed by chromatography on Sephadex G-100. The specific activity of the purest lipase fraction was 1750 micromoles fatty acid, liberated in 30 min per milligram of protein, indicating a purification of approximately 473-fold, with an overall yield of about 42%. Homogeneity of the enzyme was confirmed by rechromatography on Sephadex G-100 as well as with the gel electrophoretic and ultracentrifugal techniques. The purified enzyme gave a typical protein ultraviolet absorption spectrum with maximum absorption at 276 nm and minimum at 252 nm. The purified enzyme exhibited a single pH optimum of 8.8 and an isoelectric point near pH 5.5. Its optimum temperature was 37 C, and its optimum substrate concentration was 10%. These properties resembled those of milk lipase.  相似文献   
92.
The authors examined whether a form of implicit memory that has been unambiguously dissociated from conscious awareness--learning of spatial context on the contextual cuing task introduced by M. M. Chun and Y. Jiang (1998)--is mature in childhood as predicted by an evolutionary view of cognition. School-aged children did not show reliable learning relative to adults who performed the same version of the task or another version that slowed responses to match those of children. Thus, unreliable learning in childhood was mediated by immature implicit representations of spatial context rather than by slower baseline response speed. The present finding is inconsistent with the prediction of the evolutionary view of cognition but consistent with incomplete maturation of medial temporal lobes known to mediate contextual learning. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
93.
In this paper, we propose two information-theoretic techniques for efficiently trading off the location update and paging costs associated with mobility management in wireless cellular networks. Previous approaches always attempt to accurately convey a mobile's movement sequence and hence cannot reduce the signaling cost below the entropy bound. Our proposed techniques, however, exploit the rate distortion theory to arbitrarily reduce the update cost at the expense of an increase in the corresponding paging overhead. To this end, we describe two location tracking algorithms based on spatial quantization and temporal quantization, which first quantize the movement sequence into a smaller set of codewords and then report a compressed representation of the codeword sequence. Although the spatial quantization algorithm clusters individual cells into registration areas, the more powerful temporal quantization algorithm groups sets of consecutive movement patterns. The quantizers themselves are adaptive and periodically reconfigure to accommodate changes in the mobile's movement pattern. Simulation study with synthetic and real movement traces for both single-system and multisystem cellular networks demonstrate that the proposed algorithms can reduce the mobile's update frequency to 3-4 updates/day with reasonable paging cost, low computational complexity, storage overhead, and codebook updates.  相似文献   
94.
Software operational profile (SOP) is used in software reliability prediction,software quality assessment,performance analysis of software,test case allocation,determination of"when to stop testing,"etc.Due to the limited data resources and large efforts required to collect and convert the gathered data into point estimates,reluctance is observed by the software professionals to develop the SOP.A framework is proposed to develop SOP using fuzzy logic,which requires usage data in the form of linguistics.The resulting profile is named fuzzy software operational profile (FSOP).Based on this work,this paper proposes a generalized approach for the allocation of test cases,in which occurrence probability of operations obtained from FSOP are combined with the criticality of the operations using fuzzy inference system (FIS).Traditional methods for the allocation of test cases do not consider the application in which software operates.This is intuitively incorrect.To solve this problem,allocation of test cases with respect to software application using the FIS model is also proposed in this paper.  相似文献   
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Asterionella glacialis Castracane, a diatom species of marine phytoplankton, bloomed in the coastal waters of the northwestern Bay of Bengal during 24 March to 4 April 2004. This species dominated by 99% in cell concentration on 26 March 2004. During the bloom period, phytoplankton population density increased by three orders of magnitude, while the chlorophyll a concentration increased by 70 to 80 times relative to the pre‐bloom situation. The blooming was associated with coastal upwelling and influenced water quality. Corresponding to the blooming period, satellite sensor‐derived products of sea surface temperature (SST) and chlorophyll a data were analysed to understand the coverage and dynamics of phytoplankton in the area.  相似文献   
98.
Multiwalled carbon nanotubes were irradiated with ultra-low energy (few eV) nitrogen and hydrogen ions using a microwave discharge. These ultra-low energy plasma-ions remain confined to the nanotube walls, transferring their maximum energy to the carbon atoms, and produce extraordinary structural changes to the carbon nanotube pillars as well as within the carbon nanotubes. Conical shaped emitters and nanotube structures with nano-defects are produced that exhibit remarkable field emission with ultra-low turn-on electric field (∼0.16 V/μm) and a >300-fold increase in the maximum emission current density compared to non-irradiated nanotubes. Doping of nitrogen is also identified due to such irradiation processes.  相似文献   
99.
This paper presents a new method for incorporating imperfect FC (fault coverage) into a combinatorial model. Imperfect FC, the probability that a single malicious fault can thwart automatic recovery mechanisms, is important to accurate reliability assessment of fault-tolerant computer systems. Until recently, it was thought that the consideration of this probability necessitated a Markov model rather than the simpler (and usually faster) combinatorial model. SEA, the new approach, separates the modeling of FC failures into two terms that are multiplied to compute the system reliability. The first term, a simple product, represents the probability that no uncovered fault occurs. The second term comes from a combinatorial model which includes the covered faults that can lead to system failure. This second term can be computed from any common approach (e.g. fault tree, block diagram, digraph) which ignores the FC concept by slightly altering the component-failure probabilities. The result of this work is that reliability engineers can use their favorite software package (which ignores the FC concept) for computing reliability, and then adjust the input and output of that program slightly to produce a result which includes FC. This method applies to any system for which: the FC probabilities are constant and state-independent; the hazard rates are state-independent; and an FC failure leads to immediate system failure  相似文献   
100.
Summary  By utilising the Michael addition reaction, a wide range of acrylate functional silane coupling agents have been developed. These can be used to confer functionality on the surface of nanocomposite colloidal oxides, particularly silica which can then be incorporated into acrylic UV-curable matrices. Coatings developed from these systems provide greatly enhanced abrasion resistance, and can approach the performance of thermally-cured silicone hardcoats. The wide range of coupling agents by this method allows considerable flexibility in the design of coatings suitable for different substrates and a wide range of applications. Acknowledgements: M Sollberger, T Washer, J Blizzard and J Tonge, Dow Corning Corp.  相似文献   
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