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991.
The different steps of development of chemically induced brain tumors were investigated in rats by MRI using a superparamagnetic contrast agent, magnetite-dextran nanoparticles (MD3). Sprague-Dawley strain pregnant female rats were injected intravenously with ethynitrosourea solution at the end of pregnancy. Offspring whelped by the inoculated mother were followed. MRI examinations were performed at 0.5 T. MD3 nanoparticles were injected intravenously at a dose of 5 mg Fe kg-1 body weight 30 min before rat sacrifice. After sacrifice, histological slices were stained with hematoxylin-eosin. Relaxation times were measured at 40 MHz and 37°. MD3 nanoparticles act differently according to the step of the tumor development. Before tumor appearance, at a step characterized by the presence of abnormal cell clusters, relaxation time T2 increased significantly. The T2-weighted image showed a small increase in signal intensity in the lesion. Image contrast was improved by MD3 nanoparticles injection because of the decrease in healthy tissue signal intensity. The Tl-weighted image did not provide any additional information. In presence of a minute tumor, relaxation times decreased in tumor but increased in surrounding tissue. The Tl-weighted image showed a hypersignal on the border of an hyposignal. T2-weighted image showed a hypersignal in the same area. Signal intensity was not modified after MD3 nanoparticles injection. When new vascular capillaries developed in the tumor, MD3 nanoparticles cross into the cerebral parenchyma. Transmission electron microscopy showed magnetite crystals in this specific area on cytoplasm vesicles of glial cells and in tumor-specific membrane arrangements. On T2-weighted image, the hypersignal consisted of a well defined part and a second more fuzzy part, its signal being extinguished after MD3 nanoparticles injection. Necrotic areas and edema can be discriminated. The use of such a superparamagnetic contrast agent would be helpful in early detection of tumor development and in improving distinction of tumor mass from its vascular environment in patients. © 1998 Elsevier Science B.V. All rights reserved. 相似文献
992.
The cathodic polarization characteristics of CuO and YBa2Cu3O7- electrodes were studied in the temperature range 600 to 800°C and at oxygen partial pressures ranging from 10–4 to 0.21 atm. The activity of oxygen reduction on a CuO electrode is closely related to the electronic conductivity and the oxygen ion vacancy density in the surface layer of the electrode. The oxygen ion vacancies created in CuO by doping with Li and the modification of the electronic conductivity by adding Ag provide a new way of enhancing the activity of an oxide electrode for oxygen reduction. It is demonstrated that the rate limiting steps for oxygen reduction at high overpotential and low overpotential are oxygen adsorption and charge transfer on CuO, respectively.List of symbols
F
Faraday constant
-
f
F/RT
-
i
current
- i0
exchange current
-
k
0
intrinsic rate constant of charge transfer
-
N()
electron density with an energy level E
-
n
number of electrons
-
R
gas constant T temperature
Greek letters
transfer coefficient
-
conductivity
-
overpotential
-
energy level 相似文献
993.
Comparison of Sagnac and Mach-Zehnder ultrafast all-optical interferometric switches based on a semiconductor resonant optical nonlinearity 总被引:1,自引:0,他引:1
We present a theoretical analysis of recently demonstrated ultrafast all-optical interferometric switching devices (based on Sagnac and Mach-Zehnder interferometers) that use a large optical nonlinearity in a resonant regime. These devices achieve ~10-ps switching windows and do not require high-energy optical control pulses. We theoretically analyze and compare one Sagnac and two Mach-Zehnder switching configurations. 相似文献
994.
A shearing interferometer with a Kitty-type self-pumped phase-conjugate mirror is developed. The measurement of the focal length of a lens is demonstrated with a standard deviation of 1.5%. In addition, we measured the microdisplacement in the range of tens of micrometers with an error less than 2% by using the interferometer. 相似文献
995.
Nachman P Chen G Pinnick RG Hill SC Chang RK Mayo MW Fernandez GL 《Applied optics》1996,35(7):1069-1076
We report the design and operation of a prototype conditional-sampling spectrograph detection system that can record the fluorescence spectra of individual, micrometer-sized aerosols as they traverse an intense 488-nm intracavity laser beam. The instrument's image-intensified CCD detector is gated by elastic scattering or by undispersed fluorescence from particles that enter the spectrograph's field of view. It records spectra only from particles with preselected scattering-fluorescence levels (a fiber-optic-photomultiplier subsystem provides the gating signal). This conditional-sampling procedure reduces data-handling rates and increases the signal-to-noise ratio by restricting the system's exposures to brief periods when aerosols traverse the beam. We demonstrate these advantages by reliably capturing spectra from individual fluorescent microspheres dispersed in an airstream. The conditional-sampling procedure also permits some discrimination among different types of particles, so that spectra may be recorded from the few interesting particles present in a cloud of background aerosol. We demonstrate such discrimination by measuring spectra from selected fluorescent microspheres in a mixture of two types of microspheres, and from bacterial spores in a mixture of spores and nonfluorescent kaolin particles. 相似文献
996.
We discuss the design of a complete, compact holographic storage demonstrator based on a combination of spatial and angular multiplexing and using acousto-optic deflectors for rapid nonmechanical access. We also describe the implementation of this design and preliminary results of both the analog storage and the retrieval of 20,000 holograms in twenty 1-mm-thick layers of a lithium niobate crystal and of the digital storage and error-free retrieval of color images by using error-correcting techniques. 相似文献
997.
The effect of a carbon containing atmosphere on the microstructure and mechanical properties of Fe-32Mn-9.4Al-1C-1.27Si alloy was investigated in this work. Surface oxide nodules and grain boundary oxides were found to form on this alloy when it was annealed in carbon-containing air at 1050 °C for 1 h. The oxidation reaction was thought to be the result of the green rot attack process. This alloy was embrittled severely by the carbon-containing air through the formation of surface oxide nodules and grain boundary oxide. The carbon-containing air enhanced the oxidation rate of this alloy at 1050 °C. The structure of the oxide nodules formed on this alloy in the carbon-containing air was similar to that observed on a FeMnAl alloy heated in 1000 °C air for 24 h. 相似文献
998.
W. J. Lin T. Y. Tseng Y. Z. Wu S. P. Lin S. L. Tu H. Chang S. J. Yang I. N. Lin 《Journal of Materials Science: Materials in Electronics》1996,7(6):409-417
Growth of [0 0 /] preferentially oriented Pb1 - x
La
x
(Zr
y
Ti
z
) O3 (PLZT) thin films was carried out by using targets of either tetragonal or rhombohedral structure. The tetragonal films grew in a similar manner to the rhombohedral films. Both the substrate temperature (500 or 550 C) and oxygen pressure (0.1 mbar, 10 Pa) required stringent control in order to deposit [0 0 /]-textured PLZT thin films. The ferroelectric and fatigue properties were examined.The films deposited on YBa2Cu3O7 - x
and CeO2 coated silicon (YBCO/CeO2/Si) substrates possessed substantially lower remanent polarization than those grown on YBCO coated SrTiO3 (YBCO/STO) substrates; this is ascribed to inferior crystallinity of the PLZT/YBCO/CeO2/Si films. The remanent polarization of tetragonal PLZT films was degraded insignificantly up to 108 polarization switching cycles, whereas that of rhombohedral PLZT films was already reduced to 80% of the initial value after 108 cycles. Low endurance of rhombohedral films was ascribed to the periodic stress induced when the inclined spontaneous polarization vector (P = [111]) switched. On the other hand, high endurance of tetragonal films was explained by the fact that the spontaneous polarization vector (P = [001]) lies along the film's normal such that switch cycles cause no lateral stress. 相似文献
999.
CW Ponton M Don JJ Eggermont MD Waring B Kwong A Masuda 《Canadian Metallurgical Quarterly》1996,8(1):61-65
Separation and identification of proteins by two-dimensional (2-D) electrophoresis can be used for protein-based gene expression analysis. In this report single protein spots, from polyvinylidene difluoride blots of micropreparative E. coli 2-D gels, were rapidly and economically identified by matching their amino acid composition, estimated pI and molecular weight against all E. coli entries in the SWISS-PROT database. Thirty proteins from an E. coli 2-D map were analyzed and identities assigned. Three of the proteins were unknown. By protein sequencing analysis, 20 of the 27 proteins were correctly identified. Importantly, correct identifications showed unambiguous "correct" score patterns. While incorrect protein identifications also showed distinctive score patterns, indicating that protein must be identified by other means. These techniques allow large-scale screening of the protein complement of simple organisms, or tissues in normal and disease states. The computer program described here is accessible via the World Wide Web at URL address (http:@expasy.hcuge.ch/). 相似文献
1000.
F Fu Y Li S Qian L Lu F Chambers TE Starzl JJ Fung AW Thomson 《Canadian Metallurgical Quarterly》1996,62(5):659-665
We have shown previously that granulocyte-macrophage colony-stimulating factor-stimulated mouse bone marrow-derived MHC class II+ dendritic cell (DC) progenitors that are deficient in cell surface expression of the costimulatory molecules B7-1 (CD80) and B7-2 (CD86) can induce alloantigen-specific T-cell anergy in vitro. To test the in vivo relevance of these findings, 2 x 10(6) B10 (H2b) mouse bone marrow-derived DC progenitors (NLDC 145+, MHC class II+, B7-1dim, B7-2-/dim) that induced T-cell hyporesponsiveness in vitro were injected systemically into normal C3H (H2k) recipients. Seven days later, the mice received heterotopic heart transplants from B10 donors. No immunosuppressive treatment was given. Median graft survival time was prolonged significantly from 9.5 to 22 days. Median graft survival time was also increased, although to a lesser extent (16.5 days), in mice that received third-party (BALB/c; H2d) DC progenitors. Ex vivo analysis of host T-cell responses to donor and third-party alloantigens 7 days after the injection of DC progenitors (the time of heart transplant) revealed minimal anti-donor mixed leukocyte reaction and cytotoxic T lymphocyte reactivity. These responses were reduced substantially compared with those of spleen cells from animals pretreated with "mature" granulocyte-macrophage colony-stimulating factor + interleukin-4-stimulated DC (MHC class IIbright, B7-1+, B7-2bright), many of which rejected their heart grafts in an accelerated fashion. Among the injected donor MHC class II+ DC progenitors that migrated to recipient secondary lymphoid tissue were cells that appeared to have up-regulated cell surface B7-1 and B7-2 molecule expression. This observation may explain, at least in part, the temporary or unstable nature of the hyporesponsiveness induced by the DC progenitors in nonimmunosuppressed recipients. 相似文献