Beneficial Effects of Silicon (Si) on Sea Barley (Hordeum marinum Huds.) under Salt Stress

Silicon - Silicon (Si) plays an important role in providing beneficial effects on plant growth and yield, especially under stressful environments such as salinity. The objective of this work is to...     

Genome Editing Using CRISPR-Cas9 and Autoimmune Diseases: A Comprehensive Review

Autoimmune diseases are disorders that destruct or disrupt the body’s own tissues by its own immune system. Several studies have revealed that polymorphisms of multiple genes are involved in autoimmune diseases. Meanwhile, gene therapy has become a promising approach in autoimmune diseases, and clustered regularly interspaced palindromic repeats and CRISPR-associated protein 9 (CRISPR-Cas9) has become one of the most prominent methods. It has been shown that CRISPR-Cas9 can be applied to knock out proprotein convertase subtilisin/kexin type 9 (PCSK9) or block PCSK9, resulting in lowering low-density lipoprotein cholesterol. In other studies, it can be used to treat rare diseases such as ornithine transcarbamylase (OTC) deficiency and hereditary tyrosinemia. However, few studies on the treatment of autoimmune disease using CRISPR-Cas9 have been reported so far. In this review, we highlight the current and potential use of CRISPR-Cas9 in the management of autoimmune diseases. We summarize the potential target genes for immunomodulation using CRISPR-Cas9 in autoimmune diseases including rheumatoid arthritis (RA), inflammatory bowel diseases (IBD), systemic lupus erythematosus (SLE), multiple sclerosis (MS), type 1 diabetes mellitus (DM), psoriasis, and type 1 coeliac disease. This article will give a new perspective on understanding the use of CRISPR-Cas9 in autoimmune diseases not only through animal models but also in human models. Emerging approaches to investigate the potential target genes for CRISPR-Cas9 treatment may be promising for the tailored immunomodulation of some autoimmune diseases in the near future.     

Degradation of the antimicrobial triclocarban (TCC) with ozone

Triclocarban (TCC) has been used as an antimicrobial compound for many decades due to its sanitising properties. Recent studies showed that TCC is toxic and causes disruption to the endocrine system. In this work, ozone oxidation of TCC was studied in 70% acetonitrile:30% water solutions. Ozone degraded TCC effectively and the reaction rates increased substantially with ozone gas concentration, pH and temperature. Second-order-reaction kinetics was suitable to describe the chemical reaction. The effect of temperature was described by the Arrhenius equation and the activation energy was found equal to 31.0 kJ/mol. The stoichiometry of the reaction was one and the value of the rate constant at pH 7 was 5 × 10³ M⁻¹ s⁻¹.     

Multiwalled Carbon Nanotubes for Heterogeneous Nanocatalytic Ozonation

Multiwalled carbon nanotubes functionalized by plasma oxygen (CNTs) have been used as heterogeneous catalysts for the ozonation of methyl orange (MO) dye (CI 13025) in aqueous solutions. It was found that the addition of CNTs significantly enhanced the dye decolorization as compared to ozone alone or when activated carbon was used at the same dose as CNTs. Both the initial ozone concentration and catalyst dosage enhanced the removal of MO. However, ozone gas concentrations higher than 6 g/m³ NTP did not further improve the decolorization rates. The removal efficiency of MO increased with pH in the range 2 to 3, while a reverse trend was observed when the pH increased from 3 to 9. The addition of a radical scavenger resulted in only a limited change in the decolorization rates suggesting that molecular ozone was the main pathway by which MO decolorization occurred in solution. However, under favorable conditions for MO attraction to CNT surface (pH = 3), the decolorization rate has significantly increased. At higher pH than the pKa value of MO (3.47) and the point of zero charge of CNT (3.87), a condition that favors the electrostatic repulsion of MO from CNT, the rates were reduced in the presence of CNT as compared to ozone alone possibly due to loss of part of the supplied ozone in un-useful parallel reactions.     

Artificial Intelligence for Predicting Microsatellite Instability Based on Tumor Histomorphology: A Systematic Review

Microsatellite instability (MSI)/defective DNA mismatch repair (dMMR) is receiving more attention as a biomarker for eligibility for immune checkpoint inhibitors in advanced diseases. However, due to high costs and resource limitations, MSI/dMMR testing is not widely performed. Some attempts are in progress to predict MSI/dMMR status through histomorphological features on H&E slides using artificial intelligence (AI) technology. In this study, the potential predictive role of this new methodology was reviewed through a systematic review. Studies up to September 2021 were searched through PubMed and Embase database searches. The design and results of each study were summarized, and the risk of bias for each study was evaluated. For colorectal cancer, AI-based systems showed excellent performance with the highest standard of 0.972; for gastric and endometrial cancers they showed a relatively low but satisfactory performance, with the highest standard of 0.81 and 0.82, respectively. However, analyzing the risk of bias, most studies were evaluated at high-risk. AI-based systems showed a high potential in predicting the MSI/dMMR status of different cancer types, and particularly of colorectal cancers. Therefore, a confirmation test should be required only for the results that are positive in the AI test.     

Microstructure characterization and tensile properties of direct squeeze cast and gravity die cast 2017A wrought Al alloy

Squeeze casting is a pressurized solidification process wherein finished components can be produced in a single process from molten metal to solid utilizing re-useable die tools. This one activates different physical processes which have metallurgical repercussions on the cast material structure. Desirable features of both casting and forging are combined in this hybrid method. 2017A aluminium alloy, conventionally used for wrought products, has been successfully cast using direct squeeze casting. Squeeze casting with an applied pressure removes the defects observed in gravity die cast samples. Tensile properties and microstructures are investigated. The results show that the finer microstructure was achieved through the squeeze casting. Furthermore, higher pressures improved the fracture properties and decreased the percentage of porosity of the cast alloy. The ultimate tensile strength, the yield strength and the elongation of the squeezed cast samples improved when the squeeze pressure increased.     

Silicon Seed Priming Enhances Salt Tolerance of Barley Seedlings through Early ROS Detoxification and Stimulation of Antioxidant Defence

Silicon - Seed priming has recently gained considerable attention to induce salt tolerance in several crop plants. In the present study, we evaluated the effect of seed priming with silicon (Si)...     

Fatigue life improvements of the AISI 304 stainless steel ground surfaces by wire brushing

The surface and subsurface integrity of metallic ground components is usually characterized by an induced tensile residual stress, which has a detrimental effect on the fatigue life of these components. In particular, it tends to accelerate the initiation and growth of the fatigue cracks. In this investigation, to deliberately generate compressive residual stresses into the ground surfaces of the AISI 304 stainless steel (SS), wire brushing was applied. It was found that under the experimental conditions selected in this investigation, while the surface roughness was slightly improved by the brushing process, the surface residual stress shifted from a tensile stress (σ_‖=+450 MPa) to a compressive stress (σ_‖=−435 MPa). On the other hand, the work-hardened deformation layer was almost two times deeper after wire brushing. Concerning the fatigue life, an improvement of 26% in terms of endurance limit at 2×10⁶ cycles was realized. Scanning electron microscope (SEM) observations of the fatigue fracture location and size were carried out to explain the fatigue life improvement. It was found that the enhancement of the fatigue strength could be correlated with the distribution and location of the fatigue fracture nucleation sites. Concerning the ground surfaces, it was seen that the fatigue cracks initiated at the bottom of the grinding grooves and were particularly long (150–200 μm). However, the fatigue cracks at the brushed surfaces were shorter (20–40 μm) and appeared to initiate sideways to the plowed material caused by the wire brushing. The results of the wire-brushed surface characterization have shown that significant advantages can be realized regarding surface integrity by the application of this low-cost process compared to shot peening.     

Experimental design to enhance the surface appearance,the internal structure,and the shear stresses of injected and metallized polycarbonate/acrylonitrile–butadiene-styrene parts

Polycarbonate (PC) and acrylonitrile–butadiene–styrene (ABS) possess thermal and mechanical properties, which make them materials of choice for automotive components. These properties have presented PC/ABS as an eligible replacement for metals in automotive industry. The aim of this study was to explore a new approach to determine the optimum conditions for obtaining quality and shear stresses of the injected and metalized PC/ABS parts. For this purpose, the following six injection-molding parameters such as material temperature (137 _ma ), injection pressure (P _inj ), holding pressure (P _h ), mold temperature (T _mo ), holding time (t _h ), and cooling time (t _c ) were considered at four different values. The effect of the injection parameters studied has been analyzed through the quality and the shear stresses values of the injected and metallized PC/ABS specimens. Optical microscopic and confocal laser scanning microscope (CLSM) results demonstrate that the occurrence of some defects such as weld line, blister, poor adhesion, metal residue, sand scratch. Moreover, scanning electron microscope (SEM) findings show the presence of core defects and solidified pellets. Shear stresses vary between 3.78 and 5.8 MPa for the injected specimens and 4.5 and 6.4 MPa for the metallized specimens. The optimum injection parameter combinations were conditioned by producing flawless injected and metallized PC/ABS parts having a significant shear stress value. Thus, the optimum combinations consists of: a T_ma of 260 °C, a P_inj ranging between 30 and 50 bar, a P_h ranging between 20 and 25 bar, a T_mo of 40 °C, a t_h ranging between 8 and 14 sec. and a t_c of 25 sec. These findings may have interesting applications in automotive part industry. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137, 48384.     

Corrosion in SO2 of pure and preoxidized copper at high temperature

A kinetics and morphological study of the reaction of pure SO₂ with copper over the temperature range 500–950°C showed that only Cu₂S formed despite the fact that thermodynamic ally its formation is not expected. Alternatively, the formation of Cu₂O, expected from the Cu-O-S diagram did not occur during sulfidation; however, its evaporation was observed in an atmosphere of pure SO₂ at high temperature. Thus copper differs from others metals such as nickel or cobalt by its low reactivity with SO₂ compared to the oxidation reaction; therefore, it was possible to follow the beginning of sulfidation.