Identification and Functional Analysis of Key Autophosphorylation Residues of Arabidopsis Senescence Associated Receptor-like Kinase

Reversible protein phosphorylation mediated by protein kinases and phosphatases plays important roles in the regulation of leaf senescence. We previously reported that the senescence-associated leucine-rich repeat receptor-like kinase AtSARK autophosphorylates on both serine/threonine and tyrosine residues and functions as a positive regulator of Arabidopsis leaf senescence; the senescence-suppressed protein phosphatase SSPP interacts with and dephosphorylates the cytoplasmic domain of AtSARK, thereby negatively regulating leaf senescence. Here, 27 autophosphorylation residues of AtSARK were revealed by mass spectrometry analysis, and six of them, including two Ser, two Thr, and two Tyr residues, were further found to be important for the biological functions of AtSARK. All site-directed mutations of these six residues that resulted in decreased autophosphorylation level of AtSARK could significantly inhibit AtSARK-induced leaf senescence. In addition, mutations mimicking the dephosphorylation form of Ser384 (S384A) or the phosphorylation form of Tyr413 (Y413E) substantially reduced the interaction between AtSARK and SSPP. All results suggest that autophosphorylation of AtSARK is essential for its functions in promoting leaf senescence. The possible roles of S384 and Y413 residues in fine-tuning the interaction between AtSARK and SSPP are discussed herein.     

不同人群卫生科普需求及传播方式研究

采用查阅资料、现场观察、定量及定性调查等研究方法,以辽宁省农村居民、沈阳市社区居民及北京市农民建筑工为目标人群,了解到农村居民、城市居民、农民建筑工的卫生科普需求以及卫生科普传播方式的评价与选择存在差异,提出调整卫生科普内容、改进卫生科普传播方式及管理工作的建议.     

塔河油田AT1区块T2a3凝析气藏综合治理研究

砂岩凝析气藏见水后开发效果迅速变差,见水后治理成本高、难度大.塔河油田AT1区块T₂a³凝析气藏存在井网分布不均导致局部采速过高、储层非均质性严重、开发初期采气强度偏大、前期认识存在局限性等问题,通过捕捉生产动态中的异常信号,认为气藏存在底油,并据此形成了以油基堵剂堵水等为代表的改善开发效果的针对性技术手段.通过利用隔夹层和平面非均质性进行剩余油气挖潜,取得了较好的治理效果.     

塔河油田碳酸盐岩油藏定量化注水提高水驱效率现场应用与研究

塔河油田碳酸盐岩油藏具有埋藏深、非均质性强的特点,主要储集空间以溶孔、溶洞和裂缝为主,裂缝为主要渗流通道。随着油田能量的递减,注水做为补充能量最经济有效的方式得到大力推广。根据物质平衡原理,依托酸压曲线、注水指示曲线、能量指示曲线,对注水进行定量化计算,精细注水,明确注采关系,优化注采参数,推动油田提高水驱效率和采收率。     

The Main Progress of Perovskite Solar Cells in 2020–2021

Perovskite solar cells (PSCs) emerging as a promising photovoltaic technology with high efficiency and low manufacturing cost have attracted the attention from ...     

Direct Ink Writing of Highly Conductive MXene Frames for Tunable Electromagnetic Interference Shielding and Electromagnetic Wave?Induced Thermochromism

The highly integrated and miniaturized next-generation electronic products call for high-performance electromagnetic interfer?ence (EMI) shielding materials to ...     

Electrolyte/Structure?Dependent Cocktail Mediation Enabling High?Rate/Low?Plateau Metal Sulfide Anodes for Sodium Storage

As promising anodes for sodium-ion batteries, metal sulfides ubiquitously suffer from low-rate and high-plateau issues, greatly hindering their application in f...     

天车调度在优化钢厂物流管制中的重要作用

运用物流学中装卸搬运的知识,归纳了现代制造流程中运输环节的功能和作用,分析了炼钢厂物流系统中天车作业的特点,并对钢厂时间因素和生产工艺进行了研究与分析,从中可知天车调度在优化钢厂物流管制中的重要作用。     

热机械疲劳寿命的应变唯象描述

通过比较热机械疲劳寿命的3种不同唯象描述方法,讨论了相位差对疲劳寿命的影响,提出对疲劳寿命的唯象描述应采用总应变范围Δε_t或非弹性应变范围Δε_in,才符合工况模拟试验和疲劳寿命预测的基本条件。     

带圆孔的薄壁管多轴向低周疲劳性能的判据

在40Cr钢制成的带圆孔的薄壁管上进行拉-扭双向的低周疲劳试验,并用弹塑性有限元方法分析试件圆孔区域应力和应变分布。通过对以前的多轴向疲劳准则的分析研究,根据薄壁管拉-扭双向疲劳的试验和理论分析的结果,提出了一个新的多轴向应力疲劳的判据。它可以比较好地预测疲劳起裂寿命和裂纹位置。