Tetrandrine Inhibits Skeletal Muscle Differentiation by Blocking Autophagic Flux

Tetrandrine is well known to act as a calcium channel blocker. It is a potential candidate for a tumor chemotherapy drug without toxicity. Tetrandrine inhibits cancer cell proliferation and induces cell death through apoptosis and autophagy. As cancer patients usually experience complications with sarcopenia or muscle injury, we thus assessed the effects of tetrandrine on skeletal muscle cells. We report in this study that a low dose of tetrandrine (less than 5 μM) does not affect the proliferation of C2C12 myoblasts, but significantly inhibits myogenic differentiation. Consistently, tetrandrine inhibited muscle regeneration after BaCl₂-induced injury. Mechanistic experiments showed that tetrandrine decreased the p-mTOR level and increased the levels of LC3 and SQSTM1/p62 during differentiation. Ad-mRFP-GFP-LC3B transfection experiments revealed that the lysosomal quenching of GFP signals was suppressed by tetrandrine. Furthermore, the levels of DNM1L/Drp1, PPARGA1 and cytochrome C (Cyto C), as well as caspase 3 activation and ROS production, were decreased following tetrandrine administration, indicating that the mitochondrial network signaling was inhibited. Our results indicate that tetrandrine has dual effects on autophagic flux in myoblasts during differentiation, activation in the early stage and blockade in the late stage. The ultimate blocking of autophagic flux by tetrandrine led to the disruption of mitochondria remodeling and inhibition of myogenic differentiation. The inhibitory effects of tetrandrine on skeletal muscle differentiation may limit its application in advanced cancer patients. Thus, great attention should be paid to the clinical use of tetrandrine for cancer therapy.     

Cryogenic Exfoliation of 2D Stanene Nanosheets for Cancer Theranostics

Stanene(Sn)-based materials have been extensively applied in industrial production and daily life,but their potential biomedi-cal application remains largely un...     

负载氯喹的骨靶向纳米颗粒打破肿瘤细胞增殖与骨吸收之间的恶性循环

肿瘤细胞增殖与骨吸收之间的恶性循环加剧了骨肿瘤的进展和转移风险.为此,我们设计并制备了聚乙二醇-阿仑膦酸钠修饰的聚多巴胺(PPA)纳米粒子,并在其表面负载自噬抑制剂氯喹(CQ),期望利用该治疗载体(PPA/CQ)打破肿瘤细胞增殖与骨吸收之间的恶性循环,从而有效地治疗骨肿瘤.实验证明,PPA/CQ可以有效地富集到骨组织,...     

预钙化加速类骨磷灰石在镍钛合金表面生长的电化学阻抗谱

通过酸碱处理活化NiTi合金表面,在模拟体液中仿生生长类骨磷灰石层以改善其生物相容性.采用电化学阻抗谱研究了预钙化对加速磷灰石沉积的影响,并基于双层模型建立了电子等效电路.结果表明:随着在模拟体液中浸泡时间的延长,化学处理的NiTi合金表面类骨磷灰石不断生长,并且添加预钙化试样浸泡3 d,即可在合金表面生长出均匀完整的类骨磷灰石层,而未预钙化试样表面沉积物稀少.对应电子等效电路中,预钙化试样电阻值明显大于未预钙化试样的,显示预钙化促进了活化NiTi合金表面类骨磷灰石的生长.     

Superhydrophobic Paper-Based Microfluidic Field-Effect Transistor Biosensor Functionalized with Semiconducting Single-Walled Carbon Nanotube and DNAzyme for Hypocalcemia Diagnosis

Hypocalcemia is caused by a sharp decline in blood calcium concentration after dairy cow calving, which can lead to various diseases or even death. It is necessary to develop an inexpensive, easy-to-operate, reliable sensor to diagnose hypocalcemia. The cellulose-paper-based microfluidic field-effect biosensor is promising for point-of-care, but it has poor mechanical strength and a short service life after exposure to an aqueous solution. Octadecyltrichlorosilane (OTS), as a popular organosilane derivative, can improve the hydrophobicity of cellulose paper to overcome the shortage of cellulose paper. In this work, OTS was used to produce the superhydrophobic cellulose paper that enhances the mechanical strength and short service life of MFB, and a microfluidic field-effect biosensor (MFB) with semiconducting single-walled carbon nanotubes (SWNTs) and DNAzyme was then developed for the Ca²⁺ determination. Pyrene carboxylic acid (PCA) attached to SWNTs through a non-covalent π-π stacking interaction provided a carboxyl group that can bond with an amino group of DNAzyme. Two DNAzymes with different sensitivities were designed by changing the sequence length and cleavage site, which were functionalized with SPFET/SWNTs-PCA to form Dual-MFB, decreasing the interference of impurities in cow blood. After optimizing the detecting parameters, Dual-MFB could determine the Ca²⁺ concentration in the range of 25 μM to 5 mM, with a detection limit of 10.7 μM. The proposed Dual-MFB was applied to measure Ca²⁺ concentration in cow blood, which provided a new method to diagnose hypocalcemia after dairy cow calving.     

共轭多孔聚合物应用于近红外光热转换材料

近红外光热转换材料在光热治疗、光驱动智能器件等医学和能源领域受到广泛重视.本文以商业化芳香小分子为单体,通过一步简单的交联聚合方法制得了四种共轭多孔聚合物,并首次系统研究了它们的光热转换性能.结果表明,它们均具有灵敏的近红外光热响应性,且材料的光热转换效率与单体结构中共轭苯环数有很大关系,其中两种聚合物的光热转换效率可...     

广州市西江引水工程大直径输水钢管的吊装与焊接

广州市西江引水工程线路长,输水钢管直径大,吊装难度大,焊接质量要求高。施工过程中采用轮胎式汽车起重机吊装,现场沟槽内焊接钢管采用管道手工电弧焊接工艺及半自动焊接工艺,取得了成功。详细介绍了管道焊接工艺流程及其应注意的要点。     

株溪口电站土石围堰控制性灌浆施工工艺

在水利水电工程中,围堰的防渗效果对工程主体的施工进度和经济效益影响相当大．控制性灌浆是围堰堵漏防渗的重要方法之一。文章就株溪口水电站一期围堰控制性灌浆的施工工艺进行分析,其施工方法可供相同施工条件的围堰进行参考。     

克拉美丽气田石炭系火山岩压裂改造规律探索

准噶尔盆地克拉美丽气田石炭系火山岩储层的压裂改造是其“增储上产”和产能评价的重要手段,然而,储层发育部分连通性不佳的微细裂缝在压裂后易于形成复杂裂缝,包括转向裂缝、鱼刺状小裂缝、不同方位的多裂缝、同方位的多裂缝及纵向上的多裂缝等。为此,利用诊断复杂裂缝的近井筒效应分析技术、压裂施工过程分析技术、压后压力分析技术,进行了10井次的对比分析。结果表明：10口井压裂没有产生大的分支裂缝,但是全都产生了裂缝弯曲,其中有50%为轻微弯曲,20%为中等弯曲,30%为严重弯曲。进而得出了克拉美丽气田石炭系火山岩压裂一般不会产生较大的分支裂缝,但是总会产生不同程度的裂缝弯曲的初步认识。