The spi gene of Streptococcus pneumoniae was cloned and its nucleotide sequence was determined. It encodes a protein of 204 amino acids that is homologous to bacterial signal peptidase I proteins. The S. pneumoniae protein contains all of the conserved amino acid sequence motifs previously identified in this enzyme from both prokaryotic and eukaryotic sources. Sequence comparisons revealed several additional motifs characteristic of the enzyme. The cloned S. pneumoniae gene complemented an Escherichia coli mutant defective in its leader peptidase gene. Expression of the spi gene in S. pneumoniae appeared to be essential for viability. The cloned gene was shown to produce a polypeptide of approximately 20 kDa. Overproduction of the S. pneumoniae spi gene in an E. coli expression system gave a native protein product, soluble in the presence of a non-ionic detergent, which should be amenable to structural determination. 相似文献
The work presented here is an experimental investigation of the critical flashing flow of initially subcooled water through circumferential slits in pipes. The study provides first hand information about the prediction of leak flow rates in piping and pressure vessels retaining high temperature and high pressure. The dedicated experimental facility loop simulates the thermal hydraulic condition of Pressurized Heavy Water Reactors (PHWR). The critical flow characteristics found for varying leakage cross sections at different stagnation pressure and different degree of subcooling has been demonstrated in this paper. A marked decrease in mass flux has been found as subcooling decreases for a fixed stagnation pressure. More observation has revealed that the tighter slits or openings with very short duct as small as 0.8 cm flow length have different flow behavior than greater opening dimensions or with longer flow channels or that for nozzles. The critical flow has been seen to occur at higher pressure differentials along the flaws and prominent changes in the flow rate is reported to occur with varying dimensional parameters of the slit or cracks. 相似文献
The objective of this study was to validate retrospective caregiver interviews for diagnosing major causes of severe neonatal illness and death. A convenience sample of 149 infants aged < 28 days with one or more suspected diagnoses of interest (low birthweight/severe malnutrition, preterm birth, birth asphyxia, birth trauma, neonatal tetanus, pneumonia, meningitis, septicaemia, diarrhoea, congenital malformation or injury) was taken from patients admitted to two hospitals in Dhaka, Bangladesh. Study paediatricians performed a standardised history and physical examination and ordered laboratory and radiographic tests according to study criteria. With a median interval of 64.5 days after death or hospital discharge, caregivers of 118 (79%) infants were interviewed about their child's illness. Using reference diagnoses based on predefined clinical and laboratory criteria, the sensitivity and specificity of particular combinations of signs (algorithms) reported by the caregivers were ascertained. Sufficient numbers of children with five reference standard diagnoses were studied to validate caregiver reports. Algorithms with sensitivity and specificity > 80% were identified for neonatal tetanus, low birthweight/severe malnutrition and preterm delivery. Algorithms with specificities > 80% for birth asphyxia and pneumonia had sensitivities < 70%, or alternatively had high sensitivity with lower specificity. In settings with limited access to medical care, retrospective caregiver interviews provide a valid means of diagnosing several of the most common causes of severe neonatal illness and death. 相似文献
In the paper the fuzzy logic based multi-criteria protective relay for a three-phase power transformer is presented. Twelve criteria used to stabilize the relay are introduced and integrated by means of multi-objective decision-making methods. The introduced protection scheme contains several internal functions and coefficients which are subject to off-line pre-installation self-setting. Three unique procedures are delivered for self-adjusting the relay, and thus, making it self-organized. The results of testing show significant gains in both sensitivity and selectivity of the self-organized relay compared with traditional approaches 相似文献
Sixty-seven meniscal allografts were transplanted in the knees of 63 patients between 1988 and 1994. Before surgery, all patients experienced refractory disabling knee pain secondary to a prior total meniscectomy with advanced unicompartmental osteoarthritic changes as verified by arthroscopy. At a mean followup of 31 months (range, 1.0-5.5 years), 58 knees (86.6%) attained a good to excellent results-Twenty-one knees received isolated meniscal allografts, with 19 achieving good to excellent results (90.5%). Five knees received a medial or lateral meniscal allograft with an anterior cruciate ligament reconstruction, and 4 (80.0%) obtained good to excellent results. Thirty-four knees received a meniscal allograft in combination with either a valgus high tibial osteotomy, varus high tibial osteotomy, or varus distal femoral osteotomy to correct for preoperative varus or valgus deformities, with 29 (85.3%) attaining good to excellent results. The remaining 7 knees underwent a combined medial meniscal allograft, valgus high tibial osteotomy, and anterior cruciate ligament reconstruction with 6 (85.7%) attaining good to excellent results. The most frequent complication was a traumatic posterior horn tear in 6 knees at a mean of 21 months after surgery (range, 9-43 months), most likely the consequence of unsuccessful healing of the posterior horn of the graft. 相似文献
Blackcurrant (BC) fruits are a rich source of biologically active anthocyanins but little is known of the anthocyanin content of commonly consumed BC products or how processing affects the absorption. We report that whereas fresh and frozen whole BC were a rich source of anthocyanins, containing 897 and 642 mg (100 g FW)−1 of total anthocyanins the levels in all other products were substantially lower (0.05–10.3% of the levels in fresh fruit). Further, when the absorption and excretion of BC was assessed in volunteers consuming a portion (100 g) of frozen whole BC (642 mg total anthocyanins) and, 300 g of a BC drink made by diluting concentrated syrup (33.6 mg total anthocyanins), only small quantities of BC anthocyanins were excreted in urine (fruit, 0.053 ± 0.022%; drink, 0.036 ± 0.043%; mean percent urinary yield ± SD) and they were not detected in plasma. These data indicate that fresh and frozen BC, but not processed products, are rich sources of anthocyanins but, regardless of the food source, these anthocyanins are poorly bioavailable. 相似文献
This communication reports new results on the enthalpy of formation of pseudo-amorphous ceramic compounds constituted from silicon, carbon, oxygen, and nitrogen (SiCNO), made from the polymer route. Again, like the SiCO materials, although with one exception, the enthalpy of formation from crystalline components (SiO2 cristobalite, β-Si3N4, SiC, and excess C) is negative. Some of the alloyed oxygen–nitrogen compositions yield enthalpies that are much more negative (∼100 kJ/g·atom) in comparison with compositions that contain mainly oxygen or nitrogen (∼20 kJ/g·atom). The exception, having a N/O ratio near 2, has a positive value for the enthalpy. This may reflect the presence of nanoclusters of stoichiometric Si2N2O instead of the pseudo-amorphous nanodomain structure seen for the other samples. 相似文献
A simple N‐heterocyclic carbene (NHC) derived from 1‐methyl‐3‐ethylimidazolium tetrafluoroborate was found to be an efficient ligand for a range of copper‐catalyzed cross‐coupling reactions, leading to the formation of aromatic ethers and thioethers.