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991.
Dirk Weuster-Botz Holger Paschold Bernd Striegel Holger Gieren Maria-Regina Kula Christian Wandrey 《化学工程与技术》1994,17(2):131-137
A continuous production process has been developed up to pilot scale (300 l) for FDH production with the methylotrophic yeast Candida boidinii. A high cell mass specific FDH activity (50 U/g) is achieved by process computer controlled supply of pure methanol to operate the reactor at an optimum methanol concentration of 10 g/l. The maximum FDH spacetime yield achievable with this process control involves a residence time of 7 h. The FDH space-time yield (STY) and FDH concentration are a function of the oxygen transfer rate (OTR) of the fermenter (maximum STY = 255 U/(l h) at kLa = 870 l/h). For a reasonable compromise between high FDH space-time yield and high FDH concentration, an optimum residence time is adjustable by regulating the supply of nutrient salt solution in relation to the OTR of the fermenter. On a pilot scale (200 l continuously stirred tank reactor) roughly 4 million U of FDH were produced within 10 days at a residence time of 14.3 h. Isolation of intracellular FDH enzyme was performed using extraction with an aqueous two-phase system (PEG/K2HPO4). A technical product quality of 1.2 U/mg FDH was achieved without any chromatographic purification step. 相似文献
992.
Fracture coefficients, together with the exact origin of the crack, were extracted from data sets produced an overdeterministic
system solved by an iterative least squares method. Power series type williams equations were used in the analyses. The accuracy
evaluation indicated that the first four terms of williams equations are sufficient to describe the stress field in the vicinity
of the crack tip for both mode I and mixed mode cases. Experimental study showed that the first two terms of williams equations,
which are the same as the modified westergaard equations, cannot be used to extract mixed mode fracture parameters accurately
within the data collection region of 0.07 <r/a <0.30, where r and a are radial coordinate and crack length for an edge crack,
respectively. 相似文献
993.
By smooth catalytic hydrogenation phenylated phosphine oxides are reduced to the corresponding cyclohexyl derivatives using a Rh/Pt catalyst. In chiral phosphines the optical activity is restored. 相似文献
994.
995.
996.
Christian Cros Roselyne Feurer Michel Pouchard Paul Hagenmuller 《Materials Research Bulletin》1977,12(7):745-751
The magnetic properties of the α-MxVF3 fluorides have been investigated from 4.2 to 550 K. An antiferromagnetic behavior has been observed, the Néel temperature being in each case close to 18 K for x = 0.25. Below the ordering point a weak ferromagnetic component appears, which decreases sharply from potassium to cesium. This behavior is compared with that of the VF3 fluoride and of the homologous α-MxFeF3 phases (M = K, Rb, Cs). 相似文献
997.
998.
The polycondensation reaction between telechelic poly(dimethylsiloxanes) and α,ω-difunctional polystyrenes (or substituted polystyrenes) in toluene solution is very fast, in spite of the heterogeneous nature of the reaction medium. In the first part of the article is reported a qualitative approach of the condensation reaction in relation to the microscopic structure of the reaction medium. The second part is devoted to an investigation of the mechanical and thermomechanical behaviour of the multiblock copolymers which results from intramolecular phase separation. 相似文献
999.
1000.
H Christian 《Mikroskopie》1977,33(11-12):301-306